Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2270 and Pf1N1B4_2271 are separated by 135 nucleotides Pf1N1B4_2271 and Pf1N1B4_2272 are separated by 63 nucleotides Pf1N1B4_2272 and Pf1N1B4_2273 are separated by 27 nucleotides Pf1N1B4_2273 and Pf1N1B4_2274 are separated by 63 nucleotides
Pf1N1B4_2270 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 2,587,031 to 2,587,618
_2270
Pf1N1B4_2271 - ATPases of the AAA+ class, at 2,587,754 to 2,588,179
_2271
Pf1N1B4_2272 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 2,588,243 to 2,589,472
_2272
Pf1N1B4_2273 - hypothetical protein, at 2,589,500 to 2,589,613
_2273
Pf1N1B4_2274 - D-2-hydroxyglutarate dehydrogenase, at 2,589,677 to 2,591,071
_2274
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2587.284 kb on + strand, within Pf1N1B4_2270 at 2587.284 kb on + strand, within Pf1N1B4_2270 at 2587.284 kb on + strand, within Pf1N1B4_2270 at 2587.285 kb on - strand, within Pf1N1B4_2270 at 2587.285 kb on - strand, within Pf1N1B4_2270 at 2587.332 kb on - strand, within Pf1N1B4_2270 at 2587.353 kb on + strand, within Pf1N1B4_2270 at 2587.354 kb on - strand, within Pf1N1B4_2270 at 2587.354 kb on - strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.502 kb on - strand, within Pf1N1B4_2270 at 2587.542 kb on - strand, within Pf1N1B4_2270 at 2587.542 kb on - strand, within Pf1N1B4_2270 at 2587.640 kb on + strand at 2587.640 kb on + strand at 2587.766 kb on - strand at 2587.825 kb on + strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2588.070 kb on + strand, within Pf1N1B4_2271 at 2588.070 kb on + strand, within Pf1N1B4_2271 at 2588.070 kb on + strand, within Pf1N1B4_2271 at 2588.070 kb on + strand, within Pf1N1B4_2271 at 2588.071 kb on - strand, within Pf1N1B4_2271 at 2588.071 kb on - strand, within Pf1N1B4_2271 at 2588.071 kb on - strand, within Pf1N1B4_2271 at 2588.173 kb on + strand at 2588.174 kb on - strand at 2588.174 kb on - strand at 2588.174 kb on - strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.178 kb on - strand at 2588.178 kb on - strand at 2588.178 kb on - strand at 2588.178 kb on - strand at 2588.178 kb on - strand at 2588.238 kb on - strand at 2588.515 kb on + strand, within Pf1N1B4_2272 at 2588.530 kb on - strand, within Pf1N1B4_2272 at 2588.678 kb on + strand, within Pf1N1B4_2272 at 2588.678 kb on + strand, within Pf1N1B4_2272 at 2588.679 kb on - strand, within Pf1N1B4_2272 at 2588.679 kb on - strand, within Pf1N1B4_2272 at 2588.934 kb on + strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand, within Pf1N1B4_2272 at 2588.935 kb on - strand at 2588.940 kb on + strand, within Pf1N1B4_2272 at 2588.940 kb on + strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2588.941 kb on - strand, within Pf1N1B4_2272 at 2589.363 kb on + strand at 2589.363 kb on + strand at 2589.363 kb on + strand at 2589.451 kb on + strand at 2589.479 kb on - strand at 2589.573 kb on + strand, within Pf1N1B4_2273 at 2589.574 kb on - strand, within Pf1N1B4_2273 at 2589.594 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.599 kb on + strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.600 kb on - strand, within Pf1N1B4_2273 at 2589.761 kb on + strand at 2589.762 kb on - strand at 2589.766 kb on + strand at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.953 kb on + strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2589.954 kb on - strand, within Pf1N1B4_2274 at 2590.059 kb on - strand, within Pf1N1B4_2274 at 2590.059 kb on - strand, within Pf1N1B4_2274 at 2590.216 kb on + strand, within Pf1N1B4_2274
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,587,284 + Pf1N1B4_2270 0.43 -0.0 2,587,284 + Pf1N1B4_2270 0.43 -0.0 2,587,284 + Pf1N1B4_2270 0.43 -0.0 2,587,285 - Pf1N1B4_2270 0.43 -0.0 2,587,285 - Pf1N1B4_2270 0.43 -1.8 2,587,332 - Pf1N1B4_2270 0.51 -0.0 2,587,353 + Pf1N1B4_2270 0.55 -0.0 2,587,354 - Pf1N1B4_2270 0.55 -0.0 2,587,354 - Pf1N1B4_2270 0.55 -0.0 2,587,501 + Pf1N1B4_2270 0.80 -1.5 2,587,501 + Pf1N1B4_2270 0.80 -0.0 2,587,501 + Pf1N1B4_2270 0.80 -3.4 2,587,501 + Pf1N1B4_2270 0.80 -1.9 2,587,501 + Pf1N1B4_2270 0.80 -0.0 2,587,501 + Pf1N1B4_2270 0.80 -0.0 2,587,502 - Pf1N1B4_2270 0.80 -0.0 2,587,542 - Pf1N1B4_2270 0.87 -0.0 2,587,542 - Pf1N1B4_2270 0.87 -0.0 2,587,640 + -0.0 2,587,640 + -0.0 2,587,766 - -0.0 2,587,825 + Pf1N1B4_2271 0.17 -0.0 2,587,826 - Pf1N1B4_2271 0.17 -0.0 2,587,826 - Pf1N1B4_2271 0.17 -2.4 2,587,826 - Pf1N1B4_2271 0.17 -2.0 2,587,826 - Pf1N1B4_2271 0.17 -2.4 2,587,826 - Pf1N1B4_2271 0.17 -0.0 2,588,070 + Pf1N1B4_2271 0.74 -0.0 2,588,070 + Pf1N1B4_2271 0.74 -0.0 2,588,070 + Pf1N1B4_2271 0.74 -2.3 2,588,070 + Pf1N1B4_2271 0.74 -0.0 2,588,071 - Pf1N1B4_2271 0.74 -0.0 2,588,071 - Pf1N1B4_2271 0.74 -2.0 2,588,071 - Pf1N1B4_2271 0.74 -0.0 2,588,173 + -0.0 2,588,174 - -0.0 2,588,174 - -0.0 2,588,174 - -0.0 2,588,177 + -0.0 2,588,177 + -0.0 2,588,177 + -2.2 2,588,177 + -0.0 2,588,177 + -3.9 2,588,177 + -0.0 2,588,178 - -0.0 2,588,178 - -1.9 2,588,178 - -1.5 2,588,178 - -0.0 2,588,178 - -0.0 2,588,238 - -0.1 2,588,515 + Pf1N1B4_2272 0.22 -5.2 2,588,530 - Pf1N1B4_2272 0.23 -0.0 2,588,678 + Pf1N1B4_2272 0.35 -2.7 2,588,678 + Pf1N1B4_2272 0.35 -0.0 2,588,679 - Pf1N1B4_2272 0.35 -0.0 2,588,679 - Pf1N1B4_2272 0.35 -3.4 2,588,934 + Pf1N1B4_2272 0.56 -5.3 2,588,935 - Pf1N1B4_2272 0.56 -0.0 2,588,935 - Pf1N1B4_2272 0.56 -0.0 2,588,935 - Pf1N1B4_2272 0.56 -4.6 2,588,935 - -4.3 2,588,940 + Pf1N1B4_2272 0.57 -3.6 2,588,940 + Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,588,941 - Pf1N1B4_2272 0.57 -0.0 2,589,363 + -0.0 2,589,363 + -0.0 2,589,363 + -0.0 2,589,451 + -4.1 2,589,479 - -4.6 2,589,573 + Pf1N1B4_2273 0.64 -2.1 2,589,574 - Pf1N1B4_2273 0.65 -1.2 2,589,594 + Pf1N1B4_2273 0.82 -4.9 2,589,599 + Pf1N1B4_2273 0.87 -0.0 2,589,599 + Pf1N1B4_2273 0.87 -3.9 2,589,599 + Pf1N1B4_2273 0.87 -0.0 2,589,599 + Pf1N1B4_2273 0.87 -2.8 2,589,599 + Pf1N1B4_2273 0.87 -2.6 2,589,599 + Pf1N1B4_2273 0.87 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,600 - Pf1N1B4_2273 0.88 -2.3 2,589,600 - Pf1N1B4_2273 0.88 -4.9 2,589,600 - Pf1N1B4_2273 0.88 -0.0 2,589,761 + -2.0 2,589,762 - -1.7 2,589,766 + -0.0 2,589,953 + Pf1N1B4_2274 0.20 -2.4 2,589,953 + Pf1N1B4_2274 0.20 -0.6 2,589,953 + Pf1N1B4_2274 0.20 -0.7 2,589,954 - Pf1N1B4_2274 0.20 -1.9 2,589,954 - Pf1N1B4_2274 0.20 -0.0 2,589,954 - Pf1N1B4_2274 0.20 -0.0 2,589,954 - Pf1N1B4_2274 0.20 -0.0 2,589,954 - Pf1N1B4_2274 0.20 -0.0 2,590,059 - Pf1N1B4_2274 0.27 -0.0 2,590,059 - Pf1N1B4_2274 0.27 -1.7 2,590,216 + Pf1N1B4_2274 0.39 -1.8
Or see this region's nucleotide sequence