Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2208 and Pf1N1B4_2209 are separated by 174 nucleotides Pf1N1B4_2209 and Pf1N1B4_2210 are separated by 208 nucleotides Pf1N1B4_2210 and Pf1N1B4_2211 overlap by 14 nucleotides
Pf1N1B4_2208 - Polyphosphate kinase (EC 2.7.4.1), at 2,520,482 to 2,522,704
_2208
Pf1N1B4_2209 - Exopolyphosphatase (EC 3.6.1.11), at 2,522,879 to 2,524,381
_2209
Pf1N1B4_2210 - Glutamate transport ATP-binding protein, at 2,524,590 to 2,525,327
_2210
Pf1N1B4_2211 - Putative amino acid ABC transporter, permease protein, at 2,525,314 to 2,525,964
_2211
Position (kb)
2522
2523
2524
2525 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2521.910 kb on + strand, within Pf1N1B4_2208 at 2522.010 kb on - strand, within Pf1N1B4_2208 at 2522.042 kb on + strand, within Pf1N1B4_2208 at 2522.043 kb on - strand, within Pf1N1B4_2208 at 2522.279 kb on + strand, within Pf1N1B4_2208 at 2522.280 kb on - strand, within Pf1N1B4_2208 at 2522.280 kb on - strand, within Pf1N1B4_2208 at 2522.420 kb on + strand, within Pf1N1B4_2208 at 2522.421 kb on - strand, within Pf1N1B4_2208 at 2522.561 kb on + strand at 2522.660 kb on - strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.708 kb on - strand at 2523.011 kb on + strand at 2523.011 kb on + strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.225 kb on + strand, within Pf1N1B4_2209 at 2523.226 kb on - strand, within Pf1N1B4_2209 at 2523.226 kb on - strand, within Pf1N1B4_2209 at 2523.243 kb on + strand, within Pf1N1B4_2209 at 2523.244 kb on - strand, within Pf1N1B4_2209 at 2523.244 kb on - strand, within Pf1N1B4_2209 at 2523.249 kb on + strand, within Pf1N1B4_2209 at 2523.249 kb on + strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.474 kb on + strand, within Pf1N1B4_2209 at 2523.474 kb on + strand, within Pf1N1B4_2209 at 2523.475 kb on - strand, within Pf1N1B4_2209 at 2523.475 kb on - strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.811 kb on - strand, within Pf1N1B4_2209 at 2523.855 kb on + strand, within Pf1N1B4_2209 at 2523.856 kb on - strand, within Pf1N1B4_2209 at 2523.856 kb on - strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.868 kb on - strand, within Pf1N1B4_2209 at 2524.008 kb on + strand, within Pf1N1B4_2209 at 2524.009 kb on - strand, within Pf1N1B4_2209 at 2524.009 kb on - strand, within Pf1N1B4_2209 at 2524.151 kb on - strand, within Pf1N1B4_2209 at 2524.512 kb on + strand at 2524.548 kb on + strand at 2524.548 kb on + strand at 2524.549 kb on - strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2525.131 kb on + strand, within Pf1N1B4_2210 at 2525.132 kb on - strand, within Pf1N1B4_2210 at 2525.132 kb on - strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,521,910 + Pf1N1B4_2208 0.64 -1.3 2,522,010 - Pf1N1B4_2208 0.69 -0.0 2,522,042 + Pf1N1B4_2208 0.70 -0.0 2,522,043 - Pf1N1B4_2208 0.70 -0.0 2,522,279 + Pf1N1B4_2208 0.81 -1.9 2,522,280 - Pf1N1B4_2208 0.81 -0.8 2,522,280 - Pf1N1B4_2208 0.81 -0.0 2,522,420 + Pf1N1B4_2208 0.87 -0.0 2,522,421 - Pf1N1B4_2208 0.87 -0.0 2,522,561 + -0.0 2,522,660 - -0.0 2,522,707 + -0.0 2,522,707 + -0.0 2,522,707 + -0.0 2,522,707 + -0.0 2,522,707 + -0.8 2,522,708 - -0.0 2,523,011 + -2.0 2,523,011 + -0.0 2,523,012 - -3.4 2,523,012 - -0.0 2,523,012 - -2.0 2,523,012 - -1.5 2,523,012 - -0.0 2,523,225 + Pf1N1B4_2209 0.23 -3.1 2,523,226 - Pf1N1B4_2209 0.23 -0.0 2,523,226 - Pf1N1B4_2209 0.23 -0.0 2,523,243 + Pf1N1B4_2209 0.24 -3.9 2,523,244 - Pf1N1B4_2209 0.24 -1.6 2,523,244 - Pf1N1B4_2209 0.24 -0.0 2,523,249 + Pf1N1B4_2209 0.25 -0.0 2,523,249 + Pf1N1B4_2209 0.25 +1.0 2,523,250 - Pf1N1B4_2209 0.25 -2.8 2,523,250 - Pf1N1B4_2209 0.25 -3.0 2,523,250 - Pf1N1B4_2209 0.25 -3.2 2,523,474 + Pf1N1B4_2209 0.40 -0.0 2,523,474 + Pf1N1B4_2209 0.40 -1.0 2,523,475 - Pf1N1B4_2209 0.40 -3.3 2,523,475 - Pf1N1B4_2209 0.40 -2.0 2,523,810 + Pf1N1B4_2209 0.62 -0.0 2,523,810 + Pf1N1B4_2209 0.62 -0.0 2,523,810 + Pf1N1B4_2209 0.62 -2.0 2,523,811 - Pf1N1B4_2209 0.62 -0.0 2,523,855 + Pf1N1B4_2209 0.65 -1.0 2,523,856 - Pf1N1B4_2209 0.65 -3.2 2,523,856 - Pf1N1B4_2209 0.65 -2.3 2,523,867 + Pf1N1B4_2209 0.66 -2.6 2,523,867 + Pf1N1B4_2209 0.66 -1.0 2,523,867 + Pf1N1B4_2209 0.66 -0.0 2,523,867 + Pf1N1B4_2209 0.66 -2.0 2,523,867 + Pf1N1B4_2209 0.66 -0.0 2,523,867 + Pf1N1B4_2209 0.66 -0.0 2,523,867 + Pf1N1B4_2209 0.66 -0.0 2,523,868 - Pf1N1B4_2209 0.66 -1.0 2,524,008 + Pf1N1B4_2209 0.75 -0.0 2,524,009 - Pf1N1B4_2209 0.75 -1.6 2,524,009 - Pf1N1B4_2209 0.75 -2.3 2,524,151 - Pf1N1B4_2209 0.85 -1.6 2,524,512 + -0.0 2,524,548 + -0.0 2,524,548 + -1.4 2,524,549 - -2.5 2,524,656 + -1.1 2,524,656 + -0.0 2,524,656 + -1.4 2,524,657 - -0.0 2,524,657 - -0.0 2,524,657 - -2.1 2,524,657 - -0.0 2,525,131 + Pf1N1B4_2210 0.73 -0.0 2,525,132 - Pf1N1B4_2210 0.73 -0.0 2,525,132 - -0.0 2,525,293 + -0.0 2,525,293 + -2.3 2,525,293 + -0.0 2,525,293 + -1.6 2,525,293 + -0.0 2,525,293 + -0.0 2,525,294 - -0.0 2,525,294 - -0.0 2,525,294 - -0.0 2,525,294 - -0.0 2,525,294 - -1.4 2,525,294 - -0.0 2,525,294 - -0.0 2,525,297 + -0.0 2,525,297 + -3.9 2,525,297 + -0.0 2,525,298 - -1.8 2,525,298 - -0.0 2,525,298 - -2.3 2,525,298 - -0.0 2,525,298 - -0.0 2,525,298 - -2.9 2,525,298 - -0.0 2,525,298 - -1.8 2,525,298 - -0.0 2,525,298 - -0.0 2,525,315 + -0.0 2,525,315 + -0.0 2,525,315 + -0.0 2,525,316 - -2.3 2,525,316 - -0.0 2,525,316 - -2.5 2,525,316 - -3.1 2,525,369 + -2.8
Or see this region's nucleotide sequence