Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2009 and Pf1N1B4_2010 are separated by 211 nucleotides Pf1N1B4_2010 and Pf1N1B4_2011 are separated by 11 nucleotides
Pf1N1B4_2009 - LysR family transcriptional regulator PA5437, at 2,294,206 to 2,295,147
_2009
Pf1N1B4_2010 - Pyruvate carboxyl transferase subunit A (EC 6.4.1.1), at 2,295,359 to 2,296,774
_2010
Pf1N1B4_2011 - Pyruvate carboxyl transferase subunit B (EC 6.4.1.1), at 2,296,786 to 2,298,594
_2011
Position (kb)
2295
2296
2297 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2294.458 kb on + strand, within Pf1N1B4_2009 at 2294.459 kb on - strand, within Pf1N1B4_2009 at 2294.459 kb on - strand, within Pf1N1B4_2009 at 2294.531 kb on + strand, within Pf1N1B4_2009 at 2294.532 kb on - strand, within Pf1N1B4_2009 at 2294.532 kb on - strand, within Pf1N1B4_2009 at 2294.532 kb on - strand, within Pf1N1B4_2009 at 2294.532 kb on - strand, within Pf1N1B4_2009 at 2294.585 kb on + strand, within Pf1N1B4_2009 at 2294.586 kb on - strand, within Pf1N1B4_2009 at 2294.840 kb on + strand, within Pf1N1B4_2009 at 2294.840 kb on + strand, within Pf1N1B4_2009 at 2294.840 kb on + strand, within Pf1N1B4_2009 at 2294.841 kb on - strand, within Pf1N1B4_2009 at 2294.841 kb on - strand, within Pf1N1B4_2009 at 2294.981 kb on + strand, within Pf1N1B4_2009 at 2294.982 kb on - strand, within Pf1N1B4_2009 at 2294.999 kb on + strand, within Pf1N1B4_2009 at 2294.999 kb on + strand, within Pf1N1B4_2009 at 2294.999 kb on + strand, within Pf1N1B4_2009 at 2294.999 kb on + strand, within Pf1N1B4_2009 at 2295.000 kb on - strand, within Pf1N1B4_2009 at 2295.000 kb on - strand at 2295.000 kb on - strand, within Pf1N1B4_2009 at 2295.000 kb on - strand, within Pf1N1B4_2009 at 2295.096 kb on - strand at 2295.104 kb on - strand at 2295.104 kb on - strand at 2295.104 kb on - strand at 2295.104 kb on - strand at 2295.268 kb on - strand at 2295.364 kb on - strand at 2295.453 kb on - strand at 2295.453 kb on - strand at 2295.453 kb on - strand at 2295.453 kb on - strand at 2295.738 kb on + strand, within Pf1N1B4_2010 at 2296.115 kb on + strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.205 kb on + strand at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.206 kb on - strand, within Pf1N1B4_2010 at 2296.206 kb on - strand, within Pf1N1B4_2010 at 2296.206 kb on - strand at 2296.262 kb on - strand, within Pf1N1B4_2010 at 2296.262 kb on - strand, within Pf1N1B4_2010 at 2296.427 kb on + strand, within Pf1N1B4_2010 at 2296.427 kb on + strand, within Pf1N1B4_2010 at 2296.469 kb on + strand, within Pf1N1B4_2010 at 2296.469 kb on + strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.497 kb on - strand, within Pf1N1B4_2010 at 2296.613 kb on + strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.701 kb on - strand at 2296.701 kb on - strand at 2296.701 kb on - strand at 2296.779 kb on + strand at 2296.779 kb on + strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.953 kb on - strand at 2296.953 kb on - strand at 2297.010 kb on + strand at 2297.011 kb on - strand, within Pf1N1B4_2011 at 2297.065 kb on + strand, within Pf1N1B4_2011 at 2297.157 kb on + strand, within Pf1N1B4_2011 at 2297.183 kb on + strand, within Pf1N1B4_2011 at 2297.184 kb on - strand, within Pf1N1B4_2011 at 2297.184 kb on - strand, within Pf1N1B4_2011 at 2297.221 kb on + strand, within Pf1N1B4_2011 at 2297.221 kb on + strand, within Pf1N1B4_2011 at 2297.222 kb on - strand, within Pf1N1B4_2011 at 2297.222 kb on - strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.599 kb on + strand, within Pf1N1B4_2011 at 2297.599 kb on + strand, within Pf1N1B4_2011 at 2297.600 kb on - strand, within Pf1N1B4_2011 at 2297.600 kb on - strand at 2297.600 kb on - strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.618 kb on - strand, within Pf1N1B4_2011 at 2297.618 kb on - strand, within Pf1N1B4_2011
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,294,458 + Pf1N1B4_2009 0.27 -0.0 2,294,459 - Pf1N1B4_2009 0.27 -0.0 2,294,459 - Pf1N1B4_2009 0.27 -0.0 2,294,531 + Pf1N1B4_2009 0.35 -0.0 2,294,532 - Pf1N1B4_2009 0.35 -0.0 2,294,532 - Pf1N1B4_2009 0.35 -3.6 2,294,532 - Pf1N1B4_2009 0.35 -0.0 2,294,532 - Pf1N1B4_2009 0.35 -0.0 2,294,585 + Pf1N1B4_2009 0.40 -1.7 2,294,586 - Pf1N1B4_2009 0.40 -4.2 2,294,840 + Pf1N1B4_2009 0.67 -0.0 2,294,840 + Pf1N1B4_2009 0.67 -0.0 2,294,840 + Pf1N1B4_2009 0.67 -0.0 2,294,841 - Pf1N1B4_2009 0.67 -0.0 2,294,841 - Pf1N1B4_2009 0.67 -0.0 2,294,981 + Pf1N1B4_2009 0.82 -0.0 2,294,982 - Pf1N1B4_2009 0.82 -3.0 2,294,999 + Pf1N1B4_2009 0.84 -0.0 2,294,999 + Pf1N1B4_2009 0.84 -0.0 2,294,999 + Pf1N1B4_2009 0.84 -0.0 2,294,999 + Pf1N1B4_2009 0.84 -0.0 2,295,000 - Pf1N1B4_2009 0.84 -0.0 2,295,000 - -1.8 2,295,000 - Pf1N1B4_2009 0.84 -5.1 2,295,000 - Pf1N1B4_2009 0.84 -0.0 2,295,096 - -0.0 2,295,104 - -3.0 2,295,104 - -0.0 2,295,104 - -0.0 2,295,104 - -3.2 2,295,268 - -4.4 2,295,364 - -0.0 2,295,453 - -0.0 2,295,453 - -4.5 2,295,453 - -3.3 2,295,453 - -0.0 2,295,738 + Pf1N1B4_2010 0.27 -0.0 2,296,115 + Pf1N1B4_2010 0.53 -0.0 2,296,116 - Pf1N1B4_2010 0.53 -0.0 2,296,116 - Pf1N1B4_2010 0.53 -0.0 2,296,116 - Pf1N1B4_2010 0.53 -0.0 2,296,205 + Pf1N1B4_2010 0.60 -0.0 2,296,205 + Pf1N1B4_2010 0.60 -0.0 2,296,205 + -0.0 2,296,205 + Pf1N1B4_2010 0.60 -3.4 2,296,206 - Pf1N1B4_2010 0.60 -0.0 2,296,206 - Pf1N1B4_2010 0.60 -3.0 2,296,206 - -0.0 2,296,262 - Pf1N1B4_2010 0.64 -4.4 2,296,262 - Pf1N1B4_2010 0.64 -1.3 2,296,427 + Pf1N1B4_2010 0.75 -0.0 2,296,427 + Pf1N1B4_2010 0.75 -0.0 2,296,469 + Pf1N1B4_2010 0.78 -0.0 2,296,469 + Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -4.3 2,296,496 + Pf1N1B4_2010 0.80 -2.8 2,296,496 + Pf1N1B4_2010 0.80 -2.2 2,296,496 + Pf1N1B4_2010 0.80 -0.0 2,296,497 - Pf1N1B4_2010 0.80 -0.0 2,296,613 + Pf1N1B4_2010 0.89 -0.0 2,296,614 - Pf1N1B4_2010 0.89 -0.0 2,296,614 - Pf1N1B4_2010 0.89 -0.0 2,296,614 - Pf1N1B4_2010 0.89 -0.0 2,296,700 + -0.5 2,296,700 + -0.0 2,296,700 + -0.0 2,296,700 + -4.1 2,296,700 + -0.0 2,296,700 + -0.0 2,296,701 - -0.0 2,296,701 - -0.0 2,296,701 - -0.0 2,296,779 + -0.0 2,296,779 + -3.5 2,296,780 - -4.3 2,296,780 - -0.0 2,296,780 - -0.0 2,296,780 - -0.0 2,296,780 - -4.5 2,296,953 - -0.0 2,296,953 - -0.0 2,297,010 + -2.4 2,297,011 - Pf1N1B4_2011 0.12 -0.0 2,297,065 + Pf1N1B4_2011 0.15 -0.0 2,297,157 + Pf1N1B4_2011 0.21 -2.5 2,297,183 + Pf1N1B4_2011 0.22 -0.0 2,297,184 - Pf1N1B4_2011 0.22 -0.0 2,297,184 - Pf1N1B4_2011 0.22 -3.9 2,297,221 + Pf1N1B4_2011 0.24 -0.0 2,297,221 + Pf1N1B4_2011 0.24 -0.0 2,297,222 - Pf1N1B4_2011 0.24 -0.0 2,297,222 - Pf1N1B4_2011 0.24 -3.8 2,297,593 + Pf1N1B4_2011 0.45 -0.0 2,297,593 + Pf1N1B4_2011 0.45 -0.0 2,297,593 + Pf1N1B4_2011 0.45 -0.0 2,297,593 + Pf1N1B4_2011 0.45 -0.0 2,297,594 - Pf1N1B4_2011 0.45 -0.0 2,297,594 - Pf1N1B4_2011 0.45 -5.0 2,297,594 - Pf1N1B4_2011 0.45 -0.0 2,297,594 - Pf1N1B4_2011 0.45 -0.0 2,297,599 + Pf1N1B4_2011 0.45 -0.0 2,297,599 + Pf1N1B4_2011 0.45 -0.0 2,297,600 - Pf1N1B4_2011 0.45 -1.3 2,297,600 - -0.0 2,297,600 - Pf1N1B4_2011 0.45 -0.0 2,297,617 + Pf1N1B4_2011 0.46 -0.0 2,297,617 + Pf1N1B4_2011 0.46 -1.0 2,297,617 + Pf1N1B4_2011 0.46 -3.5 2,297,617 + Pf1N1B4_2011 0.46 -0.0 2,297,617 + Pf1N1B4_2011 0.46 -0.0 2,297,617 + Pf1N1B4_2011 0.46 -0.0 2,297,618 - Pf1N1B4_2011 0.46 -4.4 2,297,618 - Pf1N1B4_2011 0.46 -3.4
Or see this region's nucleotide sequence