Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1962

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1961 and Pf1N1B4_1962 overlap by 4 nucleotidesPf1N1B4_1962 and Pf1N1B4_1963 are separated by 14 nucleotidesPf1N1B4_1963 and Pf1N1B4_1964 are separated by 0 nucleotides Pf1N1B4_1961 - Glycosyltransferase, at 2,241,167 to 2,242,534 _1961 Pf1N1B4_1962 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 2,242,531 to 2,243,769 _1962 Pf1N1B4_1963 - O-antigen export system permease protein RfbD, at 2,243,784 to 2,244,581 _1963 Pf1N1B4_1964 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 2,244,582 to 2,246,024 _1964 Position (kb) 2242 2243 2244Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2241.687 kb on + strand, within Pf1N1B4_1961at 2241.688 kb on - strand, within Pf1N1B4_1961at 2241.822 kb on + strand, within Pf1N1B4_1961at 2241.823 kb on - strand, within Pf1N1B4_1961at 2241.823 kb on - strand, within Pf1N1B4_1961at 2241.915 kb on + strand, within Pf1N1B4_1961at 2241.916 kb on - strand, within Pf1N1B4_1961at 2241.981 kb on + strand, within Pf1N1B4_1961at 2241.981 kb on + strand, within Pf1N1B4_1961at 2241.981 kb on + strand, within Pf1N1B4_1961at 2241.981 kb on + strand, within Pf1N1B4_1961at 2241.982 kb on - strand, within Pf1N1B4_1961at 2242.014 kb on + strand, within Pf1N1B4_1961at 2242.014 kb on + strand, within Pf1N1B4_1961at 2242.014 kb on + strand, within Pf1N1B4_1961at 2242.015 kb on - strand, within Pf1N1B4_1961at 2242.015 kb on - strand, within Pf1N1B4_1961at 2242.015 kb on - strand, within Pf1N1B4_1961at 2242.015 kb on - strand, within Pf1N1B4_1961at 2242.227 kb on + strand, within Pf1N1B4_1961at 2242.506 kb on + strandat 2242.506 kb on + strandat 2242.506 kb on + strandat 2242.506 kb on + strandat 2242.507 kb on - strandat 2242.507 kb on - strandat 2242.507 kb on - strandat 2242.507 kb on - strandat 2242.507 kb on - strandat 2242.507 kb on - strandat 2242.523 kb on + strandat 2242.718 kb on + strand, within Pf1N1B4_1962at 2242.718 kb on + strand, within Pf1N1B4_1962at 2242.718 kb on + strand, within Pf1N1B4_1962at 2242.718 kb on + strand, within Pf1N1B4_1962at 2242.718 kb on + strand, within Pf1N1B4_1962at 2242.718 kb on + strand, within Pf1N1B4_1962at 2242.719 kb on - strand, within Pf1N1B4_1962at 2242.719 kb on - strand, within Pf1N1B4_1962at 2242.757 kb on + strand, within Pf1N1B4_1962at 2242.823 kb on + strand, within Pf1N1B4_1962at 2242.823 kb on + strand, within Pf1N1B4_1962at 2242.823 kb on + strand, within Pf1N1B4_1962at 2242.823 kb on + strand, within Pf1N1B4_1962at 2242.823 kb on + strand, within Pf1N1B4_1962at 2242.823 kb on + strand, within Pf1N1B4_1962at 2242.823 kb on + strandat 2242.824 kb on - strand, within Pf1N1B4_1962at 2242.824 kb on - strand, within Pf1N1B4_1962at 2243.078 kb on + strand, within Pf1N1B4_1962at 2243.078 kb on + strand, within Pf1N1B4_1962at 2243.078 kb on + strand, within Pf1N1B4_1962at 2243.078 kb on + strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.079 kb on - strand, within Pf1N1B4_1962at 2243.081 kb on + strand, within Pf1N1B4_1962at 2243.082 kb on - strand, within Pf1N1B4_1962at 2243.249 kb on + strand, within Pf1N1B4_1962at 2243.249 kb on + strand, within Pf1N1B4_1962at 2243.249 kb on + strand, within Pf1N1B4_1962at 2243.250 kb on - strand, within Pf1N1B4_1962at 2243.348 kb on + strand, within Pf1N1B4_1962at 2243.348 kb on + strand, within Pf1N1B4_1962at 2243.348 kb on + strand, within Pf1N1B4_1962at 2243.349 kb on - strand, within Pf1N1B4_1962at 2243.589 kb on + strand, within Pf1N1B4_1962at 2243.590 kb on - strand, within Pf1N1B4_1962at 2243.590 kb on - strand, within Pf1N1B4_1962at 2243.590 kb on - strand, within Pf1N1B4_1962at 2243.653 kb on + strandat 2243.654 kb on - strandat 2243.654 kb on - strandat 2243.654 kb on - strandat 2243.738 kb on + strandat 2243.747 kb on + strandat 2243.747 kb on + strandat 2243.748 kb on - strandat 2243.748 kb on - strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.773 kb on + strandat 2243.774 kb on - strandat 2243.774 kb on - strandat 2243.774 kb on - strandat 2243.774 kb on - strandat 2243.774 kb on - strandat 2243.774 kb on - strandat 2243.774 kb on - strandat 2243.774 kb on - strandat 2243.774 kb on - strandat 2243.920 kb on + strand, within Pf1N1B4_1963at 2243.921 kb on - strand, within Pf1N1B4_1963at 2243.921 kb on - strandat 2244.364 kb on + strand, within Pf1N1B4_1963at 2244.364 kb on + strand, within Pf1N1B4_1963at 2244.365 kb on - strand, within Pf1N1B4_1963at 2244.365 kb on - strand, within Pf1N1B4_1963at 2244.365 kb on - strand, within Pf1N1B4_1963at 2244.365 kb on - strand, within Pf1N1B4_1963at 2244.365 kb on - strand, within Pf1N1B4_1963at 2244.373 kb on + strand, within Pf1N1B4_1963at 2244.373 kb on + strand, within Pf1N1B4_1963at 2244.374 kb on - strand, within Pf1N1B4_1963at 2244.374 kb on - strand, within Pf1N1B4_1963at 2244.374 kb on - strand, within Pf1N1B4_1963at 2244.436 kb on + strand, within Pf1N1B4_1963at 2244.436 kb on + strandat 2244.436 kb on + strand, within Pf1N1B4_1963at 2244.437 kb on - strand, within Pf1N1B4_1963at 2244.437 kb on - strand, within Pf1N1B4_1963at 2244.637 kb on + strandat 2244.637 kb on + strandat 2244.637 kb on + strandat 2244.637 kb on + strandat 2244.637 kb on + strandat 2244.638 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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2,241,687 + Pf1N1B4_1961 0.38 -0.0
2,241,688 - Pf1N1B4_1961 0.38 -0.0
2,241,822 + Pf1N1B4_1961 0.48 -0.0
2,241,823 - Pf1N1B4_1961 0.48 -2.8
2,241,823 - Pf1N1B4_1961 0.48 -1.8
2,241,915 + Pf1N1B4_1961 0.55 -0.0
2,241,916 - Pf1N1B4_1961 0.55 -0.0
2,241,981 + Pf1N1B4_1961 0.60 -0.0
2,241,981 + Pf1N1B4_1961 0.60 -0.0
2,241,981 + Pf1N1B4_1961 0.60 -2.5
2,241,981 + Pf1N1B4_1961 0.60 -0.0
2,241,982 - Pf1N1B4_1961 0.60 -3.1
2,242,014 + Pf1N1B4_1961 0.62 -0.0
2,242,014 + Pf1N1B4_1961 0.62 -0.0
2,242,014 + Pf1N1B4_1961 0.62 -3.7
2,242,015 - Pf1N1B4_1961 0.62 -0.0
2,242,015 - Pf1N1B4_1961 0.62 -0.0
2,242,015 - Pf1N1B4_1961 0.62 -0.0
2,242,015 - Pf1N1B4_1961 0.62 -0.0
2,242,227 + Pf1N1B4_1961 0.77 -0.0
2,242,506 + -0.9
2,242,506 + -2.5
2,242,506 + -0.0
2,242,506 + -0.0
2,242,507 - -2.2
2,242,507 - -2.2
2,242,507 - -3.1
2,242,507 - -0.0
2,242,507 - -0.0
2,242,507 - -0.0
2,242,523 + -1.0
2,242,718 + Pf1N1B4_1962 0.15 -2.0
2,242,718 + Pf1N1B4_1962 0.15 -0.0
2,242,718 + Pf1N1B4_1962 0.15 -3.5
2,242,718 + Pf1N1B4_1962 0.15 -0.0
2,242,718 + Pf1N1B4_1962 0.15 -3.4
2,242,718 + Pf1N1B4_1962 0.15 -2.4
2,242,719 - Pf1N1B4_1962 0.15 -0.0
2,242,719 - Pf1N1B4_1962 0.15 -0.0
2,242,757 + Pf1N1B4_1962 0.18 -3.4
2,242,823 + Pf1N1B4_1962 0.24 -0.0
2,242,823 + Pf1N1B4_1962 0.24 -0.0
2,242,823 + Pf1N1B4_1962 0.24 -2.8
2,242,823 + Pf1N1B4_1962 0.24 -2.9
2,242,823 + Pf1N1B4_1962 0.24 -0.0
2,242,823 + Pf1N1B4_1962 0.24 -0.0
2,242,823 + -0.0
2,242,824 - Pf1N1B4_1962 0.24 -0.0
2,242,824 - Pf1N1B4_1962 0.24 -0.0
2,243,078 + Pf1N1B4_1962 0.44 -0.0
2,243,078 + Pf1N1B4_1962 0.44 -2.8
2,243,078 + Pf1N1B4_1962 0.44 -0.0
2,243,078 + Pf1N1B4_1962 0.44 -0.0
2,243,079 - Pf1N1B4_1962 0.44 -0.0
2,243,079 - Pf1N1B4_1962 0.44 -0.0
2,243,079 - Pf1N1B4_1962 0.44 -0.0
2,243,079 - Pf1N1B4_1962 0.44 -0.0
2,243,079 - Pf1N1B4_1962 0.44 -3.1
2,243,079 - Pf1N1B4_1962 0.44 -0.0
2,243,079 - Pf1N1B4_1962 0.44 -0.0
2,243,079 - Pf1N1B4_1962 0.44 -0.0
2,243,079 - Pf1N1B4_1962 0.44 -0.0
2,243,081 + Pf1N1B4_1962 0.44 -0.0
2,243,082 - Pf1N1B4_1962 0.44 -2.0
2,243,249 + Pf1N1B4_1962 0.58 -0.0
2,243,249 + Pf1N1B4_1962 0.58 -5.6
2,243,249 + Pf1N1B4_1962 0.58 -1.3
2,243,250 - Pf1N1B4_1962 0.58 -0.0
2,243,348 + Pf1N1B4_1962 0.66 -0.0
2,243,348 + Pf1N1B4_1962 0.66 -0.0
2,243,348 + Pf1N1B4_1962 0.66 -0.0
2,243,349 - Pf1N1B4_1962 0.66 -3.8
2,243,589 + Pf1N1B4_1962 0.85 +1.0
2,243,590 - Pf1N1B4_1962 0.85 -3.9
2,243,590 - Pf1N1B4_1962 0.85 -0.0
2,243,590 - Pf1N1B4_1962 0.85 -3.0
2,243,653 + -0.0
2,243,654 - -0.0
2,243,654 - -0.0
2,243,654 - -0.0
2,243,738 + -0.0
2,243,747 + -0.0
2,243,747 + -2.4
2,243,748 - -0.0
2,243,748 - -0.0
2,243,773 + -0.0
2,243,773 + -3.6
2,243,773 + -0.0
2,243,773 + -3.2
2,243,773 + -0.0
2,243,773 + -0.0
2,243,773 + -0.0
2,243,773 + -1.5
2,243,773 + -0.0
2,243,773 + -0.0
2,243,774 - -0.0
2,243,774 - -0.0
2,243,774 - -3.2
2,243,774 - -0.0
2,243,774 - -3.3
2,243,774 - -0.0
2,243,774 - -0.0
2,243,774 - -0.0
2,243,774 - -2.8
2,243,920 + Pf1N1B4_1963 0.17 -0.0
2,243,921 - Pf1N1B4_1963 0.17 -0.0
2,243,921 - -0.0
2,244,364 + Pf1N1B4_1963 0.73 -0.0
2,244,364 + Pf1N1B4_1963 0.73 -0.0
2,244,365 - Pf1N1B4_1963 0.73 -0.0
2,244,365 - Pf1N1B4_1963 0.73 -3.2
2,244,365 - Pf1N1B4_1963 0.73 -1.9
2,244,365 - Pf1N1B4_1963 0.73 -3.0
2,244,365 - Pf1N1B4_1963 0.73 -2.5
2,244,373 + Pf1N1B4_1963 0.74 -0.0
2,244,373 + Pf1N1B4_1963 0.74 -2.4
2,244,374 - Pf1N1B4_1963 0.74 -0.0
2,244,374 - Pf1N1B4_1963 0.74 -0.0
2,244,374 - Pf1N1B4_1963 0.74 -1.9
2,244,436 + Pf1N1B4_1963 0.82 -0.0
2,244,436 + -0.0
2,244,436 + Pf1N1B4_1963 0.82 -0.0
2,244,437 - Pf1N1B4_1963 0.82 -0.0
2,244,437 - Pf1N1B4_1963 0.82 -0.0
2,244,637 + -0.0
2,244,637 + -0.0
2,244,637 + -0.0
2,244,637 + -0.0
2,244,637 + -0.0
2,244,638 - -0.0

Or see this region's nucleotide sequence