Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1889 and Pf1N1B4_1890 are separated by 119 nucleotides Pf1N1B4_1890 and Pf1N1B4_1891 overlap by 4 nucleotides Pf1N1B4_1891 and Pf1N1B4_1892 are separated by 1 nucleotides
Pf1N1B4_1889 - Fused spore maturation proteins A and B, at 2,161,622 to 2,162,851
_1889
Pf1N1B4_1890 - Membrane lipoprotein lipid attachment site containing protein USSDB6D, at 2,162,971 to 2,163,597
_1890
Pf1N1B4_1891 - ABC-type transport system involved in resistance to organic solvents, periplasmic component USSDB6C, at 2,163,594 to 2,164,532
_1891
Pf1N1B4_1892 - ABC transporter ATP-binding protein USSDB6B, at 2,164,534 to 2,165,337
_1892
Position (kb)
2162
2163
2164 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.004 kb on + strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.005 kb on - strand, within Pf1N1B4_1889 at 2162.055 kb on + strand, within Pf1N1B4_1889 at 2162.055 kb on + strand, within Pf1N1B4_1889 at 2162.055 kb on + strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.056 kb on - strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.099 kb on + strand, within Pf1N1B4_1889 at 2162.100 kb on - strand, within Pf1N1B4_1889 at 2162.100 kb on - strand, within Pf1N1B4_1889 at 2162.240 kb on + strand, within Pf1N1B4_1889 at 2162.240 kb on + strand, within Pf1N1B4_1889 at 2162.241 kb on - strand, within Pf1N1B4_1889 at 2162.241 kb on - strand, within Pf1N1B4_1889 at 2162.241 kb on - strand, within Pf1N1B4_1889 at 2162.241 kb on - strand, within Pf1N1B4_1889 at 2162.385 kb on + strand, within Pf1N1B4_1889 at 2162.385 kb on + strand, within Pf1N1B4_1889 at 2162.386 kb on - strand, within Pf1N1B4_1889 at 2162.386 kb on - strand, within Pf1N1B4_1889 at 2162.402 kb on + strand, within Pf1N1B4_1889 at 2162.402 kb on + strand, within Pf1N1B4_1889 at 2162.403 kb on - strand, within Pf1N1B4_1889 at 2162.403 kb on - strand, within Pf1N1B4_1889 at 2162.462 kb on + strand, within Pf1N1B4_1889 at 2162.462 kb on + strand, within Pf1N1B4_1889 at 2162.463 kb on - strand, within Pf1N1B4_1889 at 2162.664 kb on + strand, within Pf1N1B4_1889 at 2162.665 kb on - strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.723 kb on + strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.724 kb on - strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.727 kb on + strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.728 kb on - strand, within Pf1N1B4_1889 at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.738 kb on + strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.739 kb on - strand at 2162.834 kb on + strand at 2162.918 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.953 kb on + strand at 2162.954 kb on - strand at 2162.954 kb on - strand at 2162.954 kb on - strand at 2162.972 kb on + strand at 2162.973 kb on - strand at 2162.973 kb on - strand at 2162.973 kb on - strand at 2163.201 kb on - strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.255 kb on + strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.256 kb on - strand, within Pf1N1B4_1890 at 2163.362 kb on + strand, within Pf1N1B4_1890 at 2163.362 kb on + strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.363 kb on - strand, within Pf1N1B4_1890 at 2163.509 kb on + strand, within Pf1N1B4_1890 at 2163.510 kb on - strand, within Pf1N1B4_1890 at 2163.634 kb on + strand at 2163.791 kb on + strand, within Pf1N1B4_1891 at 2163.792 kb on - strand, within Pf1N1B4_1891 at 2164.357 kb on + strand, within Pf1N1B4_1891 at 2164.358 kb on - strand, within Pf1N1B4_1891 at 2164.358 kb on - strand, within Pf1N1B4_1891 at 2164.485 kb on + strand at 2164.486 kb on - strand at 2164.486 kb on - strand at 2164.509 kb on + strand at 2164.509 kb on + strand at 2164.509 kb on + strand at 2164.509 kb on + strand at 2164.510 kb on - strand at 2164.510 kb on - strand at 2164.510 kb on - strand at 2164.510 kb on - strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.595 kb on + strand at 2164.596 kb on - strand at 2164.596 kb on - strand at 2164.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,162,004 + Pf1N1B4_1889 0.31 -0.6 2,162,004 + Pf1N1B4_1889 0.31 -1.2 2,162,004 + Pf1N1B4_1889 0.31 -0.0 2,162,004 + Pf1N1B4_1889 0.31 -0.8 2,162,004 + Pf1N1B4_1889 0.31 -0.0 2,162,004 + Pf1N1B4_1889 0.31 -0.0 2,162,004 + Pf1N1B4_1889 0.31 -0.0 2,162,004 + Pf1N1B4_1889 0.31 -0.0 2,162,004 + Pf1N1B4_1889 0.31 -0.0 2,162,005 - Pf1N1B4_1889 0.31 +0.4 2,162,005 - Pf1N1B4_1889 0.31 -0.4 2,162,005 - Pf1N1B4_1889 0.31 -0.0 2,162,005 - Pf1N1B4_1889 0.31 -0.0 2,162,005 - Pf1N1B4_1889 0.31 -1.4 2,162,005 - Pf1N1B4_1889 0.31 -0.0 2,162,005 - Pf1N1B4_1889 0.31 -0.6 2,162,005 - Pf1N1B4_1889 0.31 -1.5 2,162,055 + Pf1N1B4_1889 0.35 -0.0 2,162,055 + Pf1N1B4_1889 0.35 -0.0 2,162,055 + Pf1N1B4_1889 0.35 -1.3 2,162,056 - Pf1N1B4_1889 0.35 -0.0 2,162,056 - Pf1N1B4_1889 0.35 -0.0 2,162,056 - Pf1N1B4_1889 0.35 -1.1 2,162,056 - Pf1N1B4_1889 0.35 -2.0 2,162,056 - Pf1N1B4_1889 0.35 -0.0 2,162,056 - Pf1N1B4_1889 0.35 -0.0 2,162,099 + Pf1N1B4_1889 0.39 -2.5 2,162,099 + Pf1N1B4_1889 0.39 -0.0 2,162,099 + Pf1N1B4_1889 0.39 -1.4 2,162,099 + Pf1N1B4_1889 0.39 -2.0 2,162,099 + Pf1N1B4_1889 0.39 -1.5 2,162,100 - Pf1N1B4_1889 0.39 -0.0 2,162,100 - Pf1N1B4_1889 0.39 -0.0 2,162,240 + Pf1N1B4_1889 0.50 -2.2 2,162,240 + Pf1N1B4_1889 0.50 -0.0 2,162,241 - Pf1N1B4_1889 0.50 -1.9 2,162,241 - Pf1N1B4_1889 0.50 -0.0 2,162,241 - Pf1N1B4_1889 0.50 -0.0 2,162,241 - Pf1N1B4_1889 0.50 -2.9 2,162,385 + Pf1N1B4_1889 0.62 -0.0 2,162,385 + Pf1N1B4_1889 0.62 -0.0 2,162,386 - Pf1N1B4_1889 0.62 -0.0 2,162,386 - Pf1N1B4_1889 0.62 -0.0 2,162,402 + Pf1N1B4_1889 0.63 -0.0 2,162,402 + Pf1N1B4_1889 0.63 -0.7 2,162,403 - Pf1N1B4_1889 0.63 -0.8 2,162,403 - Pf1N1B4_1889 0.63 -0.0 2,162,462 + Pf1N1B4_1889 0.68 -0.0 2,162,462 + Pf1N1B4_1889 0.68 -0.0 2,162,463 - Pf1N1B4_1889 0.68 -2.5 2,162,664 + Pf1N1B4_1889 0.85 -1.0 2,162,665 - Pf1N1B4_1889 0.85 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -1.3 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -2.4 2,162,723 + Pf1N1B4_1889 0.90 -1.9 2,162,723 + Pf1N1B4_1889 0.90 -1.2 2,162,723 + Pf1N1B4_1889 0.90 -2.0 2,162,723 + Pf1N1B4_1889 0.90 -2.0 2,162,723 + Pf1N1B4_1889 0.90 -0.6 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -1.7 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -2.2 2,162,723 + Pf1N1B4_1889 0.90 -0.9 2,162,723 + Pf1N1B4_1889 0.90 -1.4 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,723 + Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -2.2 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -1.4 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -2.3 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -1.5 2,162,724 - Pf1N1B4_1889 0.90 -2.1 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.9 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -2.4 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -1.6 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -2.0 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,724 - Pf1N1B4_1889 0.90 -1.7 2,162,724 - Pf1N1B4_1889 0.90 -2.2 2,162,724 - Pf1N1B4_1889 0.90 -2.8 2,162,724 - Pf1N1B4_1889 0.90 -3.4 2,162,724 - Pf1N1B4_1889 0.90 -0.0 2,162,727 + Pf1N1B4_1889 0.90 -0.7 2,162,727 + Pf1N1B4_1889 0.90 -0.0 2,162,727 + Pf1N1B4_1889 0.90 -0.0 2,162,727 + Pf1N1B4_1889 0.90 -0.0 2,162,728 - Pf1N1B4_1889 0.90 -1.2 2,162,728 - -0.0 2,162,728 - Pf1N1B4_1889 0.90 -2.1 2,162,728 - Pf1N1B4_1889 0.90 -0.0 2,162,728 - Pf1N1B4_1889 0.90 -1.2 2,162,738 + -2.1 2,162,738 + -1.2 2,162,738 + -0.0 2,162,738 + -1.6 2,162,739 - -0.7 2,162,739 - -1.4 2,162,739 - -2.1 2,162,739 - -0.0 2,162,739 - -0.0 2,162,739 - -1.1 2,162,739 - -0.0 2,162,739 - -0.0 2,162,739 - -1.5 2,162,739 - -0.0 2,162,834 + -0.0 2,162,918 + -1.3 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -2.0 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -0.0 2,162,953 + -1.9 2,162,953 + -1.7 2,162,953 + -0.0 2,162,953 + -2.6 2,162,953 + -0.0 2,162,954 - -2.6 2,162,954 - -1.2 2,162,954 - -1.9 2,162,972 + -0.0 2,162,973 - -1.5 2,162,973 - -0.0 2,162,973 - -0.0 2,163,201 - Pf1N1B4_1890 0.37 -3.5 2,163,255 + Pf1N1B4_1890 0.45 -0.0 2,163,255 + Pf1N1B4_1890 0.45 -0.0 2,163,255 + Pf1N1B4_1890 0.45 -0.0 2,163,256 - Pf1N1B4_1890 0.45 -0.0 2,163,256 - Pf1N1B4_1890 0.45 -2.7 2,163,256 - Pf1N1B4_1890 0.45 -0.0 2,163,362 + Pf1N1B4_1890 0.62 -0.0 2,163,362 + Pf1N1B4_1890 0.62 -0.0 2,163,363 - Pf1N1B4_1890 0.63 -0.0 2,163,363 - Pf1N1B4_1890 0.63 -0.0 2,163,363 - Pf1N1B4_1890 0.63 -2.8 2,163,509 + Pf1N1B4_1890 0.86 -0.0 2,163,510 - Pf1N1B4_1890 0.86 -0.0 2,163,634 + -0.0 2,163,791 + Pf1N1B4_1891 0.21 -2.5 2,163,792 - Pf1N1B4_1891 0.21 -2.3 2,164,357 + Pf1N1B4_1891 0.81 -0.0 2,164,358 - Pf1N1B4_1891 0.81 -0.0 2,164,358 - Pf1N1B4_1891 0.81 -0.0 2,164,485 + -2.0 2,164,486 - -0.0 2,164,486 - -0.0 2,164,509 + -1.1 2,164,509 + -0.0 2,164,509 + -0.0 2,164,509 + -2.3 2,164,510 - -1.0 2,164,510 - -0.0 2,164,510 - -0.0 2,164,510 - -2.8 2,164,595 + -0.0 2,164,595 + -2.0 2,164,595 + -0.0 2,164,595 + -0.0 2,164,595 + -2.3 2,164,595 + -0.0 2,164,595 + -1.2 2,164,596 - -0.0 2,164,596 - -1.9 2,164,596 - -2.1
Or see this region's nucleotide sequence