Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1730 and Pf1N1B4_1731 are separated by 316 nucleotides Pf1N1B4_1731 and Pf1N1B4_1732 are separated by 133 nucleotides
Pf1N1B4_1730 - Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1), at 1,977,204 to 1,978,388
_1730
Pf1N1B4_1731 - Sensory box/GGDEF family protein, at 1,978,705 to 1,979,694
_1731
Pf1N1B4_1732 - response regulator, at 1,979,828 to 1,981,042
_1732
Position (kb)
1978
1979
1980 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1977.821 kb on + strand, within Pf1N1B4_1730 at 1977.821 kb on + strand, within Pf1N1B4_1730 at 1977.842 kb on + strand, within Pf1N1B4_1730 at 1977.843 kb on - strand, within Pf1N1B4_1730 at 1978.211 kb on + strand, within Pf1N1B4_1730 at 1978.363 kb on - strand at 1978.363 kb on - strand at 1978.484 kb on + strand at 1978.484 kb on + strand at 1978.484 kb on + strand at 1978.484 kb on + strand at 1978.484 kb on + strand at 1978.559 kb on + strand at 1978.677 kb on - strand at 1978.710 kb on + strand at 1978.713 kb on + strand at 1978.713 kb on + strand at 1978.714 kb on - strand at 1978.714 kb on - strand at 1978.714 kb on - strand at 1978.721 kb on + strand at 1978.722 kb on - strand at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.867 kb on + strand, within Pf1N1B4_1731 at 1978.868 kb on - strand, within Pf1N1B4_1731 at 1978.868 kb on - strand, within Pf1N1B4_1731 at 1978.868 kb on - strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.894 kb on + strand, within Pf1N1B4_1731 at 1978.895 kb on - strand, within Pf1N1B4_1731 at 1978.909 kb on + strand, within Pf1N1B4_1731 at 1978.910 kb on - strand, within Pf1N1B4_1731 at 1978.954 kb on + strand, within Pf1N1B4_1731 at 1978.954 kb on + strand, within Pf1N1B4_1731 at 1978.955 kb on - strand, within Pf1N1B4_1731 at 1978.955 kb on - strand, within Pf1N1B4_1731 at 1978.955 kb on - strand, within Pf1N1B4_1731 at 1978.996 kb on + strand, within Pf1N1B4_1731 at 1978.997 kb on - strand, within Pf1N1B4_1731 at 1979.205 kb on - strand, within Pf1N1B4_1731 at 1979.205 kb on - strand, within Pf1N1B4_1731 at 1979.205 kb on - strand, within Pf1N1B4_1731 at 1979.354 kb on - strand, within Pf1N1B4_1731 at 1979.535 kb on - strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.595 kb on + strand, within Pf1N1B4_1731 at 1979.596 kb on - strand at 1979.596 kb on - strand at 1979.596 kb on - strand at 1979.596 kb on - strand at 1979.596 kb on - strand at 1979.596 kb on - strand at 1979.596 kb on - strand at 1979.596 kb on - strand at 1979.599 kb on + strand at 1979.599 kb on + strand at 1979.599 kb on + strand at 1979.600 kb on - strand at 1979.600 kb on - strand at 1979.600 kb on - strand at 1979.650 kb on + strand at 1979.651 kb on - strand at 1979.683 kb on - strand at 1979.754 kb on - strand at 1979.761 kb on + strand at 1979.761 kb on + strand at 1979.761 kb on + strand at 1979.762 kb on - strand at 1979.762 kb on - strand at 1979.762 kb on - strand at 1979.762 kb on - strand at 1979.762 kb on - strand at 1979.809 kb on + strand at 1979.829 kb on + strand at 1979.830 kb on - strand at 1979.974 kb on - strand, within Pf1N1B4_1732 at 1979.995 kb on - strand, within Pf1N1B4_1732 at 1980.061 kb on - strand, within Pf1N1B4_1732 at 1980.061 kb on - strand, within Pf1N1B4_1732 at 1980.083 kb on + strand, within Pf1N1B4_1732 at 1980.083 kb on + strand, within Pf1N1B4_1732 at 1980.083 kb on + strand, within Pf1N1B4_1732 at 1980.083 kb on + strand, within Pf1N1B4_1732 at 1980.083 kb on + strand, within Pf1N1B4_1732 at 1980.084 kb on - strand, within Pf1N1B4_1732 at 1980.084 kb on - strand, within Pf1N1B4_1732 at 1980.084 kb on - strand, within Pf1N1B4_1732 at 1980.192 kb on + strand, within Pf1N1B4_1732 at 1980.193 kb on - strand, within Pf1N1B4_1732 at 1980.240 kb on + strand, within Pf1N1B4_1732 at 1980.283 kb on - strand, within Pf1N1B4_1732 at 1980.451 kb on + strand, within Pf1N1B4_1732 at 1980.451 kb on + strand, within Pf1N1B4_1732 at 1980.452 kb on - strand, within Pf1N1B4_1732 at 1980.452 kb on - strand, within Pf1N1B4_1732 at 1980.517 kb on + strand, within Pf1N1B4_1732 at 1980.517 kb on + strand, within Pf1N1B4_1732 at 1980.518 kb on - strand, within Pf1N1B4_1732 at 1980.518 kb on - strand, within Pf1N1B4_1732 at 1980.588 kb on + strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 1,977,821 + Pf1N1B4_1730 0.52 -0.0 1,977,821 + Pf1N1B4_1730 0.52 -0.0 1,977,842 + Pf1N1B4_1730 0.54 -0.0 1,977,843 - Pf1N1B4_1730 0.54 -0.0 1,978,211 + Pf1N1B4_1730 0.85 -0.0 1,978,363 - -1.7 1,978,363 - -2.6 1,978,484 + -1.5 1,978,484 + -2.2 1,978,484 + -2.1 1,978,484 + -0.0 1,978,484 + -0.0 1,978,559 + -0.0 1,978,677 - -0.0 1,978,710 + -2.5 1,978,713 + -1.8 1,978,713 + -0.8 1,978,714 - -1.6 1,978,714 - -2.8 1,978,714 - -1.0 1,978,721 + -1.3 1,978,722 - -0.0 1,978,867 + Pf1N1B4_1731 0.16 -1.6 1,978,867 + Pf1N1B4_1731 0.16 -0.0 1,978,867 + Pf1N1B4_1731 0.16 -0.1 1,978,867 + Pf1N1B4_1731 0.16 -0.0 1,978,867 + Pf1N1B4_1731 0.16 -2.4 1,978,868 - Pf1N1B4_1731 0.16 -0.0 1,978,868 - Pf1N1B4_1731 0.16 -5.6 1,978,868 - Pf1N1B4_1731 0.16 -1.1 1,978,894 + Pf1N1B4_1731 0.19 -1.7 1,978,894 + Pf1N1B4_1731 0.19 -2.4 1,978,894 + Pf1N1B4_1731 0.19 -1.9 1,978,894 + Pf1N1B4_1731 0.19 -0.0 1,978,894 + Pf1N1B4_1731 0.19 -0.0 1,978,895 - Pf1N1B4_1731 0.19 -0.0 1,978,909 + Pf1N1B4_1731 0.21 -1.0 1,978,910 - Pf1N1B4_1731 0.21 -0.0 1,978,954 + Pf1N1B4_1731 0.25 -0.0 1,978,954 + Pf1N1B4_1731 0.25 -1.0 1,978,955 - Pf1N1B4_1731 0.25 -0.0 1,978,955 - Pf1N1B4_1731 0.25 -0.0 1,978,955 - Pf1N1B4_1731 0.25 -0.2 1,978,996 + Pf1N1B4_1731 0.29 -0.0 1,978,997 - Pf1N1B4_1731 0.29 -0.0 1,979,205 - Pf1N1B4_1731 0.51 -0.0 1,979,205 - Pf1N1B4_1731 0.51 -1.3 1,979,205 - Pf1N1B4_1731 0.51 -0.0 1,979,354 - Pf1N1B4_1731 0.66 -1.3 1,979,535 - Pf1N1B4_1731 0.84 -2.6 1,979,595 + Pf1N1B4_1731 0.90 -2.1 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,595 + Pf1N1B4_1731 0.90 -2.1 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,595 + Pf1N1B4_1731 0.90 -1.2 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,595 + Pf1N1B4_1731 0.90 -0.0 1,979,596 - -0.0 1,979,596 - -4.4 1,979,596 - -0.0 1,979,596 - -0.0 1,979,596 - -1.5 1,979,596 - -1.5 1,979,596 - -0.0 1,979,596 - -0.0 1,979,599 + -1.6 1,979,599 + -0.0 1,979,599 + -1.9 1,979,600 - -0.0 1,979,600 - -0.0 1,979,600 - -0.0 1,979,650 + -3.3 1,979,651 - -0.0 1,979,683 - -0.0 1,979,754 - -0.0 1,979,761 + -0.0 1,979,761 + -0.0 1,979,761 + -0.0 1,979,762 - -1.0 1,979,762 - -0.0 1,979,762 - -2.4 1,979,762 - -0.0 1,979,762 - -0.0 1,979,809 + -2.2 1,979,829 + -0.0 1,979,830 - -0.0 1,979,974 - Pf1N1B4_1732 0.12 -0.0 1,979,995 - Pf1N1B4_1732 0.14 -0.0 1,980,061 - Pf1N1B4_1732 0.19 -2.2 1,980,061 - Pf1N1B4_1732 0.19 -2.2 1,980,083 + Pf1N1B4_1732 0.21 -0.0 1,980,083 + Pf1N1B4_1732 0.21 -0.0 1,980,083 + Pf1N1B4_1732 0.21 -0.0 1,980,083 + Pf1N1B4_1732 0.21 -1.5 1,980,083 + Pf1N1B4_1732 0.21 -1.2 1,980,084 - Pf1N1B4_1732 0.21 -2.2 1,980,084 - Pf1N1B4_1732 0.21 -0.0 1,980,084 - Pf1N1B4_1732 0.21 -1.7 1,980,192 + Pf1N1B4_1732 0.30 -2.6 1,980,193 - Pf1N1B4_1732 0.30 -0.0 1,980,240 + Pf1N1B4_1732 0.34 -0.0 1,980,283 - Pf1N1B4_1732 0.37 -1.4 1,980,451 + Pf1N1B4_1732 0.51 -1.0 1,980,451 + Pf1N1B4_1732 0.51 -2.2 1,980,452 - Pf1N1B4_1732 0.51 -1.4 1,980,452 - Pf1N1B4_1732 0.51 -1.5 1,980,517 + Pf1N1B4_1732 0.57 -2.8 1,980,517 + Pf1N1B4_1732 0.57 -0.0 1,980,518 - Pf1N1B4_1732 0.57 -3.5 1,980,518 - Pf1N1B4_1732 0.57 -1.8 1,980,588 + Pf1N1B4_1732 0.63 -2.4 1,980,589 - Pf1N1B4_1732 0.63 -2.3 1,980,589 - Pf1N1B4_1732 0.63 -0.0 1,980,589 - Pf1N1B4_1732 0.63 -0.0 1,980,589 - Pf1N1B4_1732 0.63 -0.0
Or see this region's nucleotide sequence