Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1724

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1722 and Pf1N1B4_1723 are separated by 85 nucleotidesPf1N1B4_1723 and Pf1N1B4_1724 are separated by 90 nucleotidesPf1N1B4_1724 and Pf1N1B4_1725 are separated by 3 nucleotidesPf1N1B4_1725 and Pf1N1B4_1726 are separated by 85 nucleotides Pf1N1B4_1722 - transcriptional regulator, Crp/Fnr family, at 1,969,263 to 1,969,949 _1722 Pf1N1B4_1723 - FIG028593: membrane protein, at 1,970,035 to 1,970,553 _1723 Pf1N1B4_1724 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 1,970,644 to 1,971,633 _1724 Pf1N1B4_1725 - Sulfate transport system permease protein CysW, at 1,971,637 to 1,972,434 _1725 Pf1N1B4_1726 - Sulfate transport system permease protein CysT, at 1,972,520 to 1,973,338 _1726 Position (kb) 1970 1971 1972Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 1969.654 kb on + strand, within Pf1N1B4_1722at 1969.654 kb on + strand, within Pf1N1B4_1722at 1969.654 kb on + strand, within Pf1N1B4_1722at 1969.654 kb on + strand, within Pf1N1B4_1722at 1969.654 kb on + strand, within Pf1N1B4_1722at 1969.655 kb on - strand, within Pf1N1B4_1722at 1969.767 kb on - strand, within Pf1N1B4_1722at 1969.767 kb on - strand, within Pf1N1B4_1722at 1969.792 kb on + strand, within Pf1N1B4_1722at 1969.793 kb on - strand, within Pf1N1B4_1722at 1969.793 kb on - strand, within Pf1N1B4_1722at 1969.793 kb on - strand, within Pf1N1B4_1722at 1969.793 kb on - strand, within Pf1N1B4_1722at 1969.824 kb on + strand, within Pf1N1B4_1722at 1969.825 kb on - strand, within Pf1N1B4_1722at 1969.874 kb on + strand, within Pf1N1B4_1722at 1969.874 kb on + strand, within Pf1N1B4_1722at 1969.874 kb on + strand, within Pf1N1B4_1722at 1969.875 kb on - strand, within Pf1N1B4_1722at 1969.882 kb on - strandat 1969.893 kb on + strandat 1969.893 kb on + strandat 1969.894 kb on - strandat 1969.894 kb on - strandat 1970.019 kb on + strandat 1970.020 kb on - strandat 1970.020 kb on - strandat 1970.020 kb on - strandat 1970.030 kb on + strandat 1970.030 kb on + strandat 1970.031 kb on - strandat 1970.031 kb on - strandat 1970.094 kb on + strand, within Pf1N1B4_1723at 1970.095 kb on - strand, within Pf1N1B4_1723at 1970.129 kb on + strand, within Pf1N1B4_1723at 1970.129 kb on + strand, within Pf1N1B4_1723at 1970.130 kb on - strand, within Pf1N1B4_1723at 1970.221 kb on + strand, within Pf1N1B4_1723at 1970.222 kb on - strand, within Pf1N1B4_1723at 1970.222 kb on - strand, within Pf1N1B4_1723at 1970.294 kb on + strand, within Pf1N1B4_1723at 1970.294 kb on + strand, within Pf1N1B4_1723at 1970.310 kb on + strand, within Pf1N1B4_1723at 1970.310 kb on + strand, within Pf1N1B4_1723at 1970.310 kb on + strand, within Pf1N1B4_1723at 1970.311 kb on - strand, within Pf1N1B4_1723at 1970.311 kb on - strand, within Pf1N1B4_1723at 1970.311 kb on - strand, within Pf1N1B4_1723at 1970.380 kb on + strand, within Pf1N1B4_1723at 1970.380 kb on + strand, within Pf1N1B4_1723at 1970.381 kb on - strand, within Pf1N1B4_1723at 1970.381 kb on - strand, within Pf1N1B4_1723at 1970.381 kb on - strand, within Pf1N1B4_1723at 1970.381 kb on - strand, within Pf1N1B4_1723at 1970.381 kb on - strand, within Pf1N1B4_1723at 1970.383 kb on + strand, within Pf1N1B4_1723at 1970.383 kb on + strand, within Pf1N1B4_1723at 1970.551 kb on + strandat 1970.645 kb on + strandat 1970.645 kb on + strandat 1970.645 kb on + strandat 1970.646 kb on - strandat 1970.686 kb on + strandat 1970.687 kb on - strandat 1970.809 kb on - strand, within Pf1N1B4_1724at 1970.809 kb on - strand, within Pf1N1B4_1724at 1970.933 kb on + strand, within Pf1N1B4_1724at 1971.015 kb on + strand, within Pf1N1B4_1724at 1971.016 kb on - strand, within Pf1N1B4_1724at 1971.386 kb on + strand, within Pf1N1B4_1724at 1971.386 kb on + strand, within Pf1N1B4_1724at 1971.387 kb on - strand, within Pf1N1B4_1724at 1971.387 kb on - strand, within Pf1N1B4_1724at 1971.387 kb on - strand, within Pf1N1B4_1724at 1971.387 kb on - strand, within Pf1N1B4_1724at 1971.638 kb on + strandat 1971.638 kb on + strandat 1971.638 kb on + strandat 1971.638 kb on + strandat 1971.638 kb on + strandat 1971.638 kb on + strandat 1971.639 kb on - strandat 1971.639 kb on - strandat 1971.639 kb on - strandat 1971.639 kb on - strandat 1971.639 kb on - strandat 1971.673 kb on - strandat 1971.761 kb on + strand, within Pf1N1B4_1725at 1971.761 kb on + strand, within Pf1N1B4_1725at 1971.761 kb on + strand, within Pf1N1B4_1725at 1971.762 kb on - strand, within Pf1N1B4_1725at 1971.762 kb on - strand, within Pf1N1B4_1725at 1971.762 kb on - strand, within Pf1N1B4_1725at 1971.770 kb on + strand, within Pf1N1B4_1725at 1971.770 kb on + strand, within Pf1N1B4_1725at 1971.770 kb on + strand, within Pf1N1B4_1725at 1971.770 kb on + strand, within Pf1N1B4_1725at 1971.771 kb on - strand, within Pf1N1B4_1725at 1971.771 kb on - strand, within Pf1N1B4_1725at 1971.771 kb on - strand, within Pf1N1B4_1725at 1971.771 kb on - strand, within Pf1N1B4_1725at 1971.771 kb on - strand, within Pf1N1B4_1725at 1971.884 kb on + strand, within Pf1N1B4_1725at 1971.884 kb on + strand, within Pf1N1B4_1725at 1971.884 kb on + strand, within Pf1N1B4_1725at 1971.884 kb on + strand, within Pf1N1B4_1725at 1971.884 kb on + strand, within Pf1N1B4_1725at 1971.885 kb on - strand, within Pf1N1B4_1725at 1971.885 kb on - strand, within Pf1N1B4_1725at 1971.885 kb on - strand, within Pf1N1B4_1725at 1972.132 kb on + strand, within Pf1N1B4_1725at 1972.132 kb on + strand, within Pf1N1B4_1725at 1972.132 kb on + strand, within Pf1N1B4_1725at 1972.132 kb on + strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strandat 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.133 kb on - strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.136 kb on + strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.137 kb on - strand, within Pf1N1B4_1725at 1972.217 kb on + strand, within Pf1N1B4_1725at 1972.218 kb on - strand, within Pf1N1B4_1725at 1972.218 kb on - strand, within Pf1N1B4_1725at 1972.491 kb on + strandat 1972.491 kb on + strandat 1972.492 kb on - strandat 1972.592 kb on + strandat 1972.592 kb on + strandat 1972.592 kb on + strandat 1972.593 kb on - strandat 1972.593 kb on - strandat 1972.617 kb on + strand, within Pf1N1B4_1726at 1972.618 kb on - strand, within Pf1N1B4_1726at 1972.623 kb on + strand, within Pf1N1B4_1726at 1972.624 kb on - strand, within Pf1N1B4_1726

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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1,969,654 + Pf1N1B4_1722 0.57 -0.0
1,969,654 + Pf1N1B4_1722 0.57 -1.8
1,969,654 + Pf1N1B4_1722 0.57 -0.4
1,969,654 + Pf1N1B4_1722 0.57 -0.0
1,969,654 + Pf1N1B4_1722 0.57 -0.0
1,969,655 - Pf1N1B4_1722 0.57 -0.0
1,969,767 - Pf1N1B4_1722 0.73 -0.0
1,969,767 - Pf1N1B4_1722 0.73 -0.0
1,969,792 + Pf1N1B4_1722 0.77 -0.0
1,969,793 - Pf1N1B4_1722 0.77 -0.0
1,969,793 - Pf1N1B4_1722 0.77 -0.0
1,969,793 - Pf1N1B4_1722 0.77 -2.1
1,969,793 - Pf1N1B4_1722 0.77 -0.0
1,969,824 + Pf1N1B4_1722 0.82 -2.7
1,969,825 - Pf1N1B4_1722 0.82 -3.8
1,969,874 + Pf1N1B4_1722 0.89 -0.0
1,969,874 + Pf1N1B4_1722 0.89 -0.0
1,969,874 + Pf1N1B4_1722 0.89 -0.4
1,969,875 - Pf1N1B4_1722 0.89 -0.0
1,969,882 - -0.0
1,969,893 + -0.0
1,969,893 + -0.6
1,969,894 - -2.0
1,969,894 - -0.0
1,970,019 + -0.0
1,970,020 - -1.8
1,970,020 - -0.0
1,970,020 - -0.0
1,970,030 + -0.0
1,970,030 + -0.0
1,970,031 - -0.0
1,970,031 - -2.7
1,970,094 + Pf1N1B4_1723 0.11 -0.0
1,970,095 - Pf1N1B4_1723 0.12 -1.3
1,970,129 + Pf1N1B4_1723 0.18 -3.4
1,970,129 + Pf1N1B4_1723 0.18 -0.0
1,970,130 - Pf1N1B4_1723 0.18 -0.0
1,970,221 + Pf1N1B4_1723 0.36 -0.0
1,970,222 - Pf1N1B4_1723 0.36 -2.0
1,970,222 - Pf1N1B4_1723 0.36 -0.0
1,970,294 + Pf1N1B4_1723 0.50 -0.0
1,970,294 + Pf1N1B4_1723 0.50 -1.9
1,970,310 + Pf1N1B4_1723 0.53 -0.0
1,970,310 + Pf1N1B4_1723 0.53 -2.4
1,970,310 + Pf1N1B4_1723 0.53 -0.8
1,970,311 - Pf1N1B4_1723 0.53 -3.0
1,970,311 - Pf1N1B4_1723 0.53 -0.0
1,970,311 - Pf1N1B4_1723 0.53 -3.2
1,970,380 + Pf1N1B4_1723 0.66 -0.0
1,970,380 + Pf1N1B4_1723 0.66 -0.0
1,970,381 - Pf1N1B4_1723 0.67 -0.3
1,970,381 - Pf1N1B4_1723 0.67 -1.3
1,970,381 - Pf1N1B4_1723 0.67 -0.0
1,970,381 - Pf1N1B4_1723 0.67 -1.2
1,970,381 - Pf1N1B4_1723 0.67 -0.0
1,970,383 + Pf1N1B4_1723 0.67 -0.0
1,970,383 + Pf1N1B4_1723 0.67 -0.0
1,970,551 + -1.7
1,970,645 + -1.8
1,970,645 + -1.9
1,970,645 + -0.0
1,970,646 - -0.0
1,970,686 + -0.0
1,970,687 - -0.0
1,970,809 - Pf1N1B4_1724 0.17 -0.0
1,970,809 - Pf1N1B4_1724 0.17 -3.3
1,970,933 + Pf1N1B4_1724 0.29 -0.0
1,971,015 + Pf1N1B4_1724 0.37 -0.0
1,971,016 - Pf1N1B4_1724 0.38 -3.6
1,971,386 + Pf1N1B4_1724 0.75 -0.0
1,971,386 + Pf1N1B4_1724 0.75 -0.0
1,971,387 - Pf1N1B4_1724 0.75 -2.8
1,971,387 - Pf1N1B4_1724 0.75 -0.0
1,971,387 - Pf1N1B4_1724 0.75 -0.0
1,971,387 - Pf1N1B4_1724 0.75 -0.0
1,971,638 + -0.0
1,971,638 + -3.3
1,971,638 + -0.0
1,971,638 + -0.0
1,971,638 + -0.0
1,971,638 + -4.8
1,971,639 - -0.0
1,971,639 - -0.0
1,971,639 - -0.0
1,971,639 - -0.0
1,971,639 - -0.0
1,971,673 - -0.0
1,971,761 + Pf1N1B4_1725 0.16 -3.3
1,971,761 + Pf1N1B4_1725 0.16 -0.0
1,971,761 + Pf1N1B4_1725 0.16 -3.3
1,971,762 - Pf1N1B4_1725 0.16 -0.0
1,971,762 - Pf1N1B4_1725 0.16 -0.0
1,971,762 - Pf1N1B4_1725 0.16 -0.0
1,971,770 + Pf1N1B4_1725 0.17 -4.0
1,971,770 + Pf1N1B4_1725 0.17 -0.0
1,971,770 + Pf1N1B4_1725 0.17 -4.4
1,971,770 + Pf1N1B4_1725 0.17 -4.0
1,971,771 - Pf1N1B4_1725 0.17 -0.0
1,971,771 - Pf1N1B4_1725 0.17 -5.0
1,971,771 - Pf1N1B4_1725 0.17 -0.0
1,971,771 - Pf1N1B4_1725 0.17 -0.0
1,971,771 - Pf1N1B4_1725 0.17 -0.0
1,971,884 + Pf1N1B4_1725 0.31 -0.0
1,971,884 + Pf1N1B4_1725 0.31 -3.8
1,971,884 + Pf1N1B4_1725 0.31 -3.6
1,971,884 + Pf1N1B4_1725 0.31 -2.5
1,971,884 + Pf1N1B4_1725 0.31 -0.0
1,971,885 - Pf1N1B4_1725 0.31 -0.0
1,971,885 - Pf1N1B4_1725 0.31 -2.8
1,971,885 - Pf1N1B4_1725 0.31 -0.0
1,972,132 + Pf1N1B4_1725 0.62 -2.4
1,972,132 + Pf1N1B4_1725 0.62 -0.0
1,972,132 + Pf1N1B4_1725 0.62 -0.0
1,972,132 + Pf1N1B4_1725 0.62 -3.8
1,972,133 - Pf1N1B4_1725 0.62 -0.0
1,972,133 - Pf1N1B4_1725 0.62 -6.2
1,972,133 - Pf1N1B4_1725 0.62 -0.0
1,972,133 - Pf1N1B4_1725 0.62 -3.6
1,972,133 - Pf1N1B4_1725 0.62 -0.0
1,972,133 - Pf1N1B4_1725 0.62 -4.0
1,972,133 - Pf1N1B4_1725 0.62 -0.0
1,972,133 - Pf1N1B4_1725 0.62 -5.3
1,972,133 - Pf1N1B4_1725 0.62 -3.8
1,972,133 - -0.0
1,972,133 - Pf1N1B4_1725 0.62 -4.1
1,972,133 - Pf1N1B4_1725 0.62 -3.5
1,972,133 - Pf1N1B4_1725 0.62 -0.0
1,972,133 - Pf1N1B4_1725 0.62 -0.0
1,972,133 - Pf1N1B4_1725 0.62 -0.0
1,972,133 - Pf1N1B4_1725 0.62 -3.9
1,972,133 - Pf1N1B4_1725 0.62 -2.9
1,972,133 - Pf1N1B4_1725 0.62 -0.0
1,972,133 - Pf1N1B4_1725 0.62 -4.2
1,972,136 + Pf1N1B4_1725 0.63 -0.0
1,972,136 + Pf1N1B4_1725 0.63 -3.4
1,972,136 + Pf1N1B4_1725 0.63 -0.0
1,972,136 + Pf1N1B4_1725 0.63 -0.0
1,972,136 + Pf1N1B4_1725 0.63 -0.0
1,972,136 + Pf1N1B4_1725 0.63 -0.0
1,972,136 + Pf1N1B4_1725 0.63 -0.0
1,972,136 + Pf1N1B4_1725 0.63 -6.3
1,972,136 + Pf1N1B4_1725 0.63 -0.0
1,972,137 - Pf1N1B4_1725 0.63 -0.0
1,972,137 - Pf1N1B4_1725 0.63 -0.0
1,972,137 - Pf1N1B4_1725 0.63 -0.0
1,972,137 - Pf1N1B4_1725 0.63 -3.5
1,972,137 - Pf1N1B4_1725 0.63 -0.0
1,972,137 - Pf1N1B4_1725 0.63 -4.5
1,972,137 - Pf1N1B4_1725 0.63 -0.0
1,972,137 - Pf1N1B4_1725 0.63 -0.0
1,972,137 - Pf1N1B4_1725 0.63 -3.2
1,972,137 - Pf1N1B4_1725 0.63 -3.1
1,972,217 + Pf1N1B4_1725 0.73 -0.0
1,972,218 - Pf1N1B4_1725 0.73 -0.0
1,972,218 - Pf1N1B4_1725 0.73 -5.4
1,972,491 + -0.0
1,972,491 + -0.0
1,972,492 - -0.0
1,972,592 + -0.0
1,972,592 + -0.0
1,972,592 + -0.0
1,972,593 - -5.3
1,972,593 - -0.0
1,972,617 + Pf1N1B4_1726 0.12 -0.0
1,972,618 - Pf1N1B4_1726 0.12 -0.0
1,972,623 + Pf1N1B4_1726 0.13 -0.0
1,972,624 - Pf1N1B4_1726 0.13 -3.9

Or see this region's nucleotide sequence