Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1625

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1623 and Pf1N1B4_1624 are separated by 9 nucleotidesPf1N1B4_1624 and Pf1N1B4_1625 are separated by 99 nucleotidesPf1N1B4_1625 and Pf1N1B4_1626 are separated by 0 nucleotidesPf1N1B4_1626 and Pf1N1B4_1627 overlap by 1 nucleotides Pf1N1B4_1623 - Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-), at 1,867,729 to 1,868,499 _1623 Pf1N1B4_1624 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data), at 1,868,509 to 1,869,246 _1624 Pf1N1B4_1625 - FIG00956267: hypothetical protein, at 1,869,346 to 1,869,603 _1625 Pf1N1B4_1626 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 1,869,604 to 1,870,242 _1626 Pf1N1B4_1627 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 1,870,242 to 1,870,877 _1627 Position (kb) 1869 1870Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1868.355 kb on + strand, within Pf1N1B4_1623at 1868.519 kb on + strandat 1868.519 kb on + strandat 1868.520 kb on - strandat 1868.520 kb on - strandat 1868.520 kb on - strandat 1868.520 kb on - strandat 1868.567 kb on + strandat 1868.567 kb on + strandat 1868.567 kb on + strandat 1868.567 kb on + strandat 1868.568 kb on - strandat 1868.568 kb on - strandat 1868.568 kb on - strandat 1868.568 kb on - strandat 1868.568 kb on - strandat 1868.568 kb on - strandat 1868.568 kb on - strandat 1868.702 kb on + strand, within Pf1N1B4_1624at 1868.703 kb on - strand, within Pf1N1B4_1624at 1868.703 kb on - strand, within Pf1N1B4_1624at 1868.749 kb on - strand, within Pf1N1B4_1624at 1868.750 kb on + strand, within Pf1N1B4_1624at 1868.751 kb on - strand, within Pf1N1B4_1624at 1868.948 kb on + strand, within Pf1N1B4_1624at 1868.948 kb on + strand, within Pf1N1B4_1624at 1868.948 kb on + strand, within Pf1N1B4_1624at 1868.948 kb on + strand, within Pf1N1B4_1624at 1868.948 kb on + strand, within Pf1N1B4_1624at 1868.949 kb on - strand, within Pf1N1B4_1624at 1868.949 kb on - strand, within Pf1N1B4_1624at 1868.949 kb on - strand, within Pf1N1B4_1624at 1868.969 kb on + strand, within Pf1N1B4_1624at 1868.969 kb on + strand, within Pf1N1B4_1624at 1868.970 kb on - strand, within Pf1N1B4_1624at 1868.970 kb on - strand, within Pf1N1B4_1624at 1868.970 kb on - strand, within Pf1N1B4_1624at 1868.970 kb on - strand, within Pf1N1B4_1624at 1869.052 kb on + strand, within Pf1N1B4_1624at 1869.052 kb on + strand, within Pf1N1B4_1624at 1869.052 kb on + strand, within Pf1N1B4_1624at 1869.052 kb on + strand, within Pf1N1B4_1624at 1869.052 kb on + strand, within Pf1N1B4_1624at 1869.053 kb on - strand, within Pf1N1B4_1624at 1869.053 kb on - strand, within Pf1N1B4_1624at 1869.053 kb on - strand, within Pf1N1B4_1624at 1869.219 kb on + strandat 1869.219 kb on + strandat 1869.237 kb on + strandat 1869.237 kb on + strandat 1869.237 kb on + strandat 1869.237 kb on + strandat 1869.238 kb on - strandat 1869.238 kb on - strandat 1869.241 kb on + strandat 1869.241 kb on + strandat 1869.241 kb on + strandat 1869.241 kb on + strandat 1869.241 kb on + strandat 1869.241 kb on + strandat 1869.241 kb on + strandat 1869.241 kb on + strandat 1869.242 kb on - strandat 1869.242 kb on - strandat 1869.242 kb on - strandat 1869.242 kb on - strandat 1869.242 kb on - strandat 1869.242 kb on - strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.282 kb on + strandat 1869.283 kb on - strandat 1869.283 kb on - strandat 1869.304 kb on + strandat 1869.347 kb on + strandat 1869.348 kb on - strandat 1869.434 kb on + strand, within Pf1N1B4_1625at 1869.434 kb on + strand, within Pf1N1B4_1625at 1869.434 kb on + strand, within Pf1N1B4_1625at 1869.434 kb on + strand, within Pf1N1B4_1625at 1869.434 kb on + strand, within Pf1N1B4_1625at 1869.434 kb on + strand, within Pf1N1B4_1625at 1869.434 kb on + strand, within Pf1N1B4_1625at 1869.434 kb on + strand, within Pf1N1B4_1625at 1869.435 kb on - strand, within Pf1N1B4_1625at 1869.435 kb on - strand, within Pf1N1B4_1625at 1869.435 kb on - strand, within Pf1N1B4_1625at 1869.437 kb on + strand, within Pf1N1B4_1625at 1869.438 kb on - strand, within Pf1N1B4_1625at 1869.438 kb on - strand, within Pf1N1B4_1625at 1869.606 kb on - strandat 1869.776 kb on + strand, within Pf1N1B4_1626at 1869.776 kb on + strand, within Pf1N1B4_1626at 1869.776 kb on + strandat 1869.776 kb on + strand, within Pf1N1B4_1626at 1869.776 kb on + strand, within Pf1N1B4_1626at 1869.776 kb on + strand, within Pf1N1B4_1626at 1869.776 kb on + strand, within Pf1N1B4_1626at 1869.776 kb on + strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.777 kb on - strand, within Pf1N1B4_1626at 1869.779 kb on + strand, within Pf1N1B4_1626at 1869.779 kb on + strand, within Pf1N1B4_1626at 1869.779 kb on + strandat 1869.779 kb on + strand, within Pf1N1B4_1626at 1869.779 kb on + strand, within Pf1N1B4_1626at 1869.779 kb on + strand, within Pf1N1B4_1626at 1869.779 kb on + strand, within Pf1N1B4_1626at 1869.780 kb on - strand, within Pf1N1B4_1626at 1869.780 kb on - strand, within Pf1N1B4_1626at 1869.780 kb on - strand, within Pf1N1B4_1626at 1869.780 kb on - strand, within Pf1N1B4_1626at 1869.780 kb on - strand, within Pf1N1B4_1626at 1869.795 kb on - strand, within Pf1N1B4_1626at 1869.795 kb on - strand, within Pf1N1B4_1626at 1869.795 kb on - strand, within Pf1N1B4_1626at 1869.795 kb on - strand, within Pf1N1B4_1626at 1869.795 kb on - strand, within Pf1N1B4_1626at 1869.839 kb on - strand, within Pf1N1B4_1626at 1869.839 kb on - strand, within Pf1N1B4_1626at 1869.839 kb on - strand, within Pf1N1B4_1626at 1870.466 kb on + strand, within Pf1N1B4_1627at 1870.468 kb on + strand, within Pf1N1B4_1627at 1870.468 kb on + strand, within Pf1N1B4_1627at 1870.469 kb on - strand, within Pf1N1B4_1627at 1870.469 kb on - strand, within Pf1N1B4_1627at 1870.546 kb on + strand, within Pf1N1B4_1627at 1870.546 kb on + strand, within Pf1N1B4_1627at 1870.546 kb on + strand, within Pf1N1B4_1627at 1870.547 kb on - strand, within Pf1N1B4_1627at 1870.547 kb on - strand, within Pf1N1B4_1627at 1870.547 kb on - strand, within Pf1N1B4_1627at 1870.547 kb on - strand, within Pf1N1B4_1627

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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1,868,355 + Pf1N1B4_1623 0.81 -2.9
1,868,519 + -3.9
1,868,519 + -0.0
1,868,520 - -0.0
1,868,520 - -1.0
1,868,520 - -0.0
1,868,520 - -0.0
1,868,567 + -3.0
1,868,567 + -2.3
1,868,567 + -2.8
1,868,567 + -0.0
1,868,568 - -0.0
1,868,568 - -2.8
1,868,568 - -3.9
1,868,568 - -4.9
1,868,568 - -0.0
1,868,568 - -0.0
1,868,568 - -0.0
1,868,702 + Pf1N1B4_1624 0.26 -4.1
1,868,703 - Pf1N1B4_1624 0.26 -0.0
1,868,703 - Pf1N1B4_1624 0.26 -0.0
1,868,749 - Pf1N1B4_1624 0.33 -0.0
1,868,750 + Pf1N1B4_1624 0.33 -0.0
1,868,751 - Pf1N1B4_1624 0.33 -0.0
1,868,948 + Pf1N1B4_1624 0.59 -3.8
1,868,948 + Pf1N1B4_1624 0.59 -3.0
1,868,948 + Pf1N1B4_1624 0.59 -4.1
1,868,948 + Pf1N1B4_1624 0.59 -0.0
1,868,948 + Pf1N1B4_1624 0.59 -4.7
1,868,949 - Pf1N1B4_1624 0.60 -0.0
1,868,949 - Pf1N1B4_1624 0.60 -0.0
1,868,949 - Pf1N1B4_1624 0.60 -0.0
1,868,969 + Pf1N1B4_1624 0.62 -0.0
1,868,969 + Pf1N1B4_1624 0.62 -5.1
1,868,970 - Pf1N1B4_1624 0.62 -4.0
1,868,970 - Pf1N1B4_1624 0.62 -0.0
1,868,970 - Pf1N1B4_1624 0.62 -0.0
1,868,970 - Pf1N1B4_1624 0.62 -0.0
1,869,052 + Pf1N1B4_1624 0.74 -0.0
1,869,052 + Pf1N1B4_1624 0.74 -0.0
1,869,052 + Pf1N1B4_1624 0.74 -2.0
1,869,052 + Pf1N1B4_1624 0.74 -4.1
1,869,052 + Pf1N1B4_1624 0.74 -0.0
1,869,053 - Pf1N1B4_1624 0.74 -1.9
1,869,053 - Pf1N1B4_1624 0.74 -0.0
1,869,053 - Pf1N1B4_1624 0.74 -0.0
1,869,219 + -4.1
1,869,219 + -0.0
1,869,237 + -0.0
1,869,237 + -1.8
1,869,237 + -3.5
1,869,237 + -4.9
1,869,238 - -3.8
1,869,238 - -0.0
1,869,241 + -0.0
1,869,241 + -0.0
1,869,241 + -0.0
1,869,241 + -0.0
1,869,241 + -1.0
1,869,241 + -0.0
1,869,241 + -0.0
1,869,241 + -0.0
1,869,242 - -0.0
1,869,242 - -0.0
1,869,242 - -3.3
1,869,242 - -4.7
1,869,242 - -2.8
1,869,242 - -0.0
1,869,282 + -2.6
1,869,282 + -2.5
1,869,282 + -4.5
1,869,282 + -5.3
1,869,282 + -3.1
1,869,282 + -0.0
1,869,282 + -4.9
1,869,282 + -2.7
1,869,282 + -0.0
1,869,282 + -5.2
1,869,282 + -3.8
1,869,282 + -2.3
1,869,282 + -0.0
1,869,283 - -1.1
1,869,283 - -0.0
1,869,304 + -0.0
1,869,347 + -3.1
1,869,348 - -0.0
1,869,434 + Pf1N1B4_1625 0.34 -0.0
1,869,434 + Pf1N1B4_1625 0.34 -3.4
1,869,434 + Pf1N1B4_1625 0.34 -0.0
1,869,434 + Pf1N1B4_1625 0.34 -4.0
1,869,434 + Pf1N1B4_1625 0.34 -4.5
1,869,434 + Pf1N1B4_1625 0.34 -0.0
1,869,434 + Pf1N1B4_1625 0.34 -0.0
1,869,434 + Pf1N1B4_1625 0.34 -0.0
1,869,435 - Pf1N1B4_1625 0.34 -2.2
1,869,435 - Pf1N1B4_1625 0.34 -0.0
1,869,435 - Pf1N1B4_1625 0.34 -2.3
1,869,437 + Pf1N1B4_1625 0.35 +1.0
1,869,438 - Pf1N1B4_1625 0.36 -0.9
1,869,438 - Pf1N1B4_1625 0.36 -0.0
1,869,606 - -2.1
1,869,776 + Pf1N1B4_1626 0.27 -0.0
1,869,776 + Pf1N1B4_1626 0.27 -3.3
1,869,776 + -3.5
1,869,776 + Pf1N1B4_1626 0.27 -1.8
1,869,776 + Pf1N1B4_1626 0.27 -3.4
1,869,776 + Pf1N1B4_1626 0.27 -0.0
1,869,776 + Pf1N1B4_1626 0.27 -0.0
1,869,776 + Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -1.9
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -3.2
1,869,777 - Pf1N1B4_1626 0.27 -2.8
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -1.6
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,777 - Pf1N1B4_1626 0.27 -0.0
1,869,779 + Pf1N1B4_1626 0.27 -4.1
1,869,779 + Pf1N1B4_1626 0.27 -4.6
1,869,779 + -3.2
1,869,779 + Pf1N1B4_1626 0.27 -2.1
1,869,779 + Pf1N1B4_1626 0.27 -0.0
1,869,779 + Pf1N1B4_1626 0.27 -2.8
1,869,779 + Pf1N1B4_1626 0.27 -5.4
1,869,780 - Pf1N1B4_1626 0.28 -4.3
1,869,780 - Pf1N1B4_1626 0.28 -2.5
1,869,780 - Pf1N1B4_1626 0.28 -3.0
1,869,780 - Pf1N1B4_1626 0.28 -0.0
1,869,780 - Pf1N1B4_1626 0.28 -4.4
1,869,795 - Pf1N1B4_1626 0.30 -0.0
1,869,795 - Pf1N1B4_1626 0.30 -0.0
1,869,795 - Pf1N1B4_1626 0.30 -5.4
1,869,795 - Pf1N1B4_1626 0.30 -5.2
1,869,795 - Pf1N1B4_1626 0.30 -2.3
1,869,839 - Pf1N1B4_1626 0.37 -4.0
1,869,839 - Pf1N1B4_1626 0.37 -5.7
1,869,839 - Pf1N1B4_1626 0.37 -0.0
1,870,466 + Pf1N1B4_1627 0.35 -0.0
1,870,468 + Pf1N1B4_1627 0.36 -3.0
1,870,468 + Pf1N1B4_1627 0.36 -1.0
1,870,469 - Pf1N1B4_1627 0.36 -0.0
1,870,469 - Pf1N1B4_1627 0.36 -5.2
1,870,546 + Pf1N1B4_1627 0.48 -4.7
1,870,546 + Pf1N1B4_1627 0.48 -3.9
1,870,546 + Pf1N1B4_1627 0.48 -4.9
1,870,547 - Pf1N1B4_1627 0.48 -0.0
1,870,547 - Pf1N1B4_1627 0.48 -0.0
1,870,547 - Pf1N1B4_1627 0.48 -3.4
1,870,547 - Pf1N1B4_1627 0.48 -0.0

Or see this region's nucleotide sequence