Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1214

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1212 and Pf1N1B4_1213 are separated by 84 nucleotidesPf1N1B4_1213 and Pf1N1B4_1214 are separated by 138 nucleotidesPf1N1B4_1214 and Pf1N1B4_1215 are separated by 80 nucleotidesPf1N1B4_1215 and Pf1N1B4_1216 are separated by 149 nucleotides Pf1N1B4_1212 - Predicted flavin reductase RutF in novel pyrimidine catabolism pathway, at 1,375,077 to 1,375,379 _1212 Pf1N1B4_1213 - Transcriptional regulator, at 1,375,464 to 1,375,739 _1213 Pf1N1B4_1214 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 1,375,878 to 1,376,408 _1214 Pf1N1B4_1215 - Transcriptional regulator, ArsR family, at 1,376,489 to 1,376,851 _1215 Pf1N1B4_1216 - Outer membrane component of tripartite multidrug resistance system, at 1,377,001 to 1,378,431 _1216 Position (kb) 1375 1376 1377Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1374.897 kb on - strandat 1375.320 kb on + strand, within Pf1N1B4_1212at 1375.321 kb on - strand, within Pf1N1B4_1212at 1375.335 kb on + strand, within Pf1N1B4_1212at 1375.335 kb on + strand, within Pf1N1B4_1212at 1375.335 kb on + strand, within Pf1N1B4_1212at 1375.335 kb on + strand, within Pf1N1B4_1212at 1375.335 kb on + strand, within Pf1N1B4_1212at 1375.335 kb on + strand, within Pf1N1B4_1212at 1375.336 kb on - strand, within Pf1N1B4_1212at 1375.336 kb on - strand, within Pf1N1B4_1212at 1375.469 kb on - strandat 1375.494 kb on - strand, within Pf1N1B4_1213at 1375.494 kb on - strand, within Pf1N1B4_1213at 1375.495 kb on + strand, within Pf1N1B4_1213at 1375.496 kb on - strand, within Pf1N1B4_1213at 1375.496 kb on - strand, within Pf1N1B4_1213at 1375.502 kb on + strand, within Pf1N1B4_1213at 1375.502 kb on + strand, within Pf1N1B4_1213at 1375.503 kb on - strand, within Pf1N1B4_1213at 1375.503 kb on - strand, within Pf1N1B4_1213at 1375.503 kb on - strand, within Pf1N1B4_1213at 1375.503 kb on - strand, within Pf1N1B4_1213at 1375.503 kb on - strand, within Pf1N1B4_1213at 1375.514 kb on + strand, within Pf1N1B4_1213at 1375.515 kb on - strand, within Pf1N1B4_1213at 1375.515 kb on - strand, within Pf1N1B4_1213at 1375.515 kb on - strand, within Pf1N1B4_1213at 1375.515 kb on - strand, within Pf1N1B4_1213at 1375.547 kb on + strand, within Pf1N1B4_1213at 1375.717 kb on + strandat 1375.717 kb on + strandat 1375.718 kb on - strandat 1375.718 kb on - strandat 1375.817 kb on - strandat 1375.817 kb on - strandat 1375.817 kb on - strandat 1375.826 kb on + strandat 1375.994 kb on - strand, within Pf1N1B4_1214at 1376.069 kb on + strand, within Pf1N1B4_1214at 1376.069 kb on + strand, within Pf1N1B4_1214at 1376.069 kb on + strand, within Pf1N1B4_1214at 1376.069 kb on + strand, within Pf1N1B4_1214at 1376.069 kb on + strand, within Pf1N1B4_1214at 1376.069 kb on + strand, within Pf1N1B4_1214at 1376.069 kb on + strand, within Pf1N1B4_1214at 1376.069 kb on + strand, within Pf1N1B4_1214at 1376.070 kb on - strand, within Pf1N1B4_1214at 1376.070 kb on - strand, within Pf1N1B4_1214at 1376.070 kb on - strand, within Pf1N1B4_1214at 1376.070 kb on - strand, within Pf1N1B4_1214at 1376.070 kb on - strand, within Pf1N1B4_1214at 1376.070 kb on - strand, within Pf1N1B4_1214at 1376.070 kb on - strand, within Pf1N1B4_1214at 1376.070 kb on - strand, within Pf1N1B4_1214at 1376.101 kb on + strand, within Pf1N1B4_1214at 1376.293 kb on + strand, within Pf1N1B4_1214at 1376.293 kb on + strand, within Pf1N1B4_1214at 1376.293 kb on + strand, within Pf1N1B4_1214at 1376.364 kb on + strandat 1376.365 kb on - strandat 1376.409 kb on - strandat 1376.409 kb on - strandat 1376.478 kb on - strandat 1376.478 kb on - strandat 1376.478 kb on - strandat 1376.478 kb on - strandat 1376.478 kb on - strandat 1376.641 kb on - strand, within Pf1N1B4_1215at 1376.811 kb on - strand, within Pf1N1B4_1215at 1376.814 kb on - strand, within Pf1N1B4_1215at 1376.851 kb on + strandat 1376.851 kb on + strandat 1376.852 kb on - strandat 1376.852 kb on - strandat 1376.852 kb on - strandat 1376.852 kb on - strandat 1376.852 kb on - strandat 1376.852 kb on - strandat 1376.852 kb on - strandat 1376.852 kb on - strandat 1376.856 kb on - strandat 1376.856 kb on - strandat 1376.856 kb on - strandat 1376.856 kb on - strandat 1376.860 kb on - strandat 1376.860 kb on - strandat 1376.860 kb on - strandat 1376.872 kb on - strandat 1376.872 kb on - strandat 1376.872 kb on - strandat 1376.874 kb on - strandat 1376.874 kb on - strandat 1376.874 kb on - strandat 1376.890 kb on - strandat 1376.893 kb on - strandat 1376.893 kb on - strandat 1376.893 kb on - strandat 1376.904 kb on - strandat 1376.917 kb on - strandat 1376.917 kb on - strandat 1376.936 kb on - strandat 1376.936 kb on - strandat 1376.965 kb on - strandat 1376.965 kb on - strandat 1376.965 kb on - strandat 1376.965 kb on - strandat 1377.144 kb on + strandat 1377.144 kb on + strand, within Pf1N1B4_1216at 1377.144 kb on + strand, within Pf1N1B4_1216at 1377.144 kb on + strand, within Pf1N1B4_1216at 1377.144 kb on + strand, within Pf1N1B4_1216at 1377.145 kb on - strand, within Pf1N1B4_1216at 1377.145 kb on - strand, within Pf1N1B4_1216at 1377.145 kb on - strand, within Pf1N1B4_1216at 1377.145 kb on - strand, within Pf1N1B4_1216at 1377.145 kb on - strand, within Pf1N1B4_1216at 1377.145 kb on - strandat 1377.145 kb on - strand, within Pf1N1B4_1216at 1377.162 kb on - strand, within Pf1N1B4_1216at 1377.162 kb on - strand, within Pf1N1B4_1216at 1377.182 kb on + strand, within Pf1N1B4_1216at 1377.182 kb on + strand, within Pf1N1B4_1216at 1377.183 kb on - strand, within Pf1N1B4_1216at 1377.183 kb on - strand, within Pf1N1B4_1216at 1377.183 kb on - strand, within Pf1N1B4_1216at 1377.289 kb on + strand, within Pf1N1B4_1216at 1377.290 kb on - strand, within Pf1N1B4_1216at 1377.290 kb on - strand, within Pf1N1B4_1216at 1377.299 kb on + strand, within Pf1N1B4_1216at 1377.300 kb on - strand, within Pf1N1B4_1216at 1377.315 kb on + strand, within Pf1N1B4_1216at 1377.316 kb on - strand, within Pf1N1B4_1216

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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1,374,897 - -2.1
1,375,320 + Pf1N1B4_1212 0.80 -1.9
1,375,321 - Pf1N1B4_1212 0.81 -0.0
1,375,335 + Pf1N1B4_1212 0.85 -5.4
1,375,335 + Pf1N1B4_1212 0.85 -2.2
1,375,335 + Pf1N1B4_1212 0.85 -0.0
1,375,335 + Pf1N1B4_1212 0.85 -0.0
1,375,335 + Pf1N1B4_1212 0.85 -0.0
1,375,335 + Pf1N1B4_1212 0.85 -0.8
1,375,336 - Pf1N1B4_1212 0.85 -0.0
1,375,336 - Pf1N1B4_1212 0.85 -0.0
1,375,469 - -1.4
1,375,494 - Pf1N1B4_1213 0.11 -0.0
1,375,494 - Pf1N1B4_1213 0.11 -0.0
1,375,495 + Pf1N1B4_1213 0.11 -0.0
1,375,496 - Pf1N1B4_1213 0.12 -0.0
1,375,496 - Pf1N1B4_1213 0.12 -2.4
1,375,502 + Pf1N1B4_1213 0.14 -1.7
1,375,502 + Pf1N1B4_1213 0.14 -0.0
1,375,503 - Pf1N1B4_1213 0.14 -0.0
1,375,503 - Pf1N1B4_1213 0.14 -0.0
1,375,503 - Pf1N1B4_1213 0.14 -0.0
1,375,503 - Pf1N1B4_1213 0.14 -0.0
1,375,503 - Pf1N1B4_1213 0.14 -0.7
1,375,514 + Pf1N1B4_1213 0.18 -2.4
1,375,515 - Pf1N1B4_1213 0.18 -0.0
1,375,515 - Pf1N1B4_1213 0.18 -0.0
1,375,515 - Pf1N1B4_1213 0.18 -0.0
1,375,515 - Pf1N1B4_1213 0.18 -0.7
1,375,547 + Pf1N1B4_1213 0.30 -4.1
1,375,717 + -1.0
1,375,717 + -0.0
1,375,718 - -0.0
1,375,718 - -0.0
1,375,817 - -0.0
1,375,817 - -0.6
1,375,817 - -1.5
1,375,826 + -0.0
1,375,994 - Pf1N1B4_1214 0.22 -0.0
1,376,069 + Pf1N1B4_1214 0.36 -0.0
1,376,069 + Pf1N1B4_1214 0.36 -0.0
1,376,069 + Pf1N1B4_1214 0.36 -0.0
1,376,069 + Pf1N1B4_1214 0.36 -0.0
1,376,069 + Pf1N1B4_1214 0.36 -0.0
1,376,069 + Pf1N1B4_1214 0.36 -2.0
1,376,069 + Pf1N1B4_1214 0.36 -0.0
1,376,069 + Pf1N1B4_1214 0.36 -2.2
1,376,070 - Pf1N1B4_1214 0.36 -1.6
1,376,070 - Pf1N1B4_1214 0.36 -0.0
1,376,070 - Pf1N1B4_1214 0.36 -0.0
1,376,070 - Pf1N1B4_1214 0.36 -2.8
1,376,070 - Pf1N1B4_1214 0.36 -0.0
1,376,070 - Pf1N1B4_1214 0.36 -3.1
1,376,070 - Pf1N1B4_1214 0.36 -0.0
1,376,070 - Pf1N1B4_1214 0.36 -2.7
1,376,101 + Pf1N1B4_1214 0.42 -1.8
1,376,293 + Pf1N1B4_1214 0.78 -0.0
1,376,293 + Pf1N1B4_1214 0.78 -0.0
1,376,293 + Pf1N1B4_1214 0.78 -0.0
1,376,364 + -0.0
1,376,365 - -0.0
1,376,409 - -1.7
1,376,409 - -0.0
1,376,478 - -1.9
1,376,478 - -2.1
1,376,478 - -0.0
1,376,478 - -1.3
1,376,478 - -0.0
1,376,641 - Pf1N1B4_1215 0.42 -0.0
1,376,811 - Pf1N1B4_1215 0.89 -0.0
1,376,814 - Pf1N1B4_1215 0.90 -0.0
1,376,851 + -0.0
1,376,851 + -0.0
1,376,852 - -0.0
1,376,852 - -0.0
1,376,852 - -0.0
1,376,852 - -0.0
1,376,852 - -0.0
1,376,852 - -0.0
1,376,852 - -0.0
1,376,852 - -0.0
1,376,856 - -0.0
1,376,856 - -3.8
1,376,856 - -0.0
1,376,856 - -0.0
1,376,860 - -0.0
1,376,860 - -4.0
1,376,860 - -2.6
1,376,872 - -0.0
1,376,872 - -0.0
1,376,872 - -0.0
1,376,874 - -0.0
1,376,874 - -2.1
1,376,874 - -0.0
1,376,890 - -0.8
1,376,893 - -0.0
1,376,893 - -2.8
1,376,893 - -0.0
1,376,904 - -0.0
1,376,917 - -0.0
1,376,917 - -0.0
1,376,936 - -3.5
1,376,936 - -1.7
1,376,965 - -0.0
1,376,965 - -0.0
1,376,965 - -0.0
1,376,965 - -0.0
1,377,144 + -1.0
1,377,144 + Pf1N1B4_1216 0.10 -0.0
1,377,144 + Pf1N1B4_1216 0.10 -0.0
1,377,144 + Pf1N1B4_1216 0.10 -0.0
1,377,144 + Pf1N1B4_1216 0.10 -2.5
1,377,145 - Pf1N1B4_1216 0.10 -0.0
1,377,145 - Pf1N1B4_1216 0.10 -1.4
1,377,145 - Pf1N1B4_1216 0.10 -0.0
1,377,145 - Pf1N1B4_1216 0.10 -0.0
1,377,145 - Pf1N1B4_1216 0.10 -0.0
1,377,145 - -0.8
1,377,145 - Pf1N1B4_1216 0.10 -0.0
1,377,162 - Pf1N1B4_1216 0.11 -1.9
1,377,162 - Pf1N1B4_1216 0.11 -2.3
1,377,182 + Pf1N1B4_1216 0.13 -0.0
1,377,182 + Pf1N1B4_1216 0.13 -0.7
1,377,183 - Pf1N1B4_1216 0.13 -0.0
1,377,183 - Pf1N1B4_1216 0.13 -0.0
1,377,183 - Pf1N1B4_1216 0.13 -1.9
1,377,289 + Pf1N1B4_1216 0.20 -1.9
1,377,290 - Pf1N1B4_1216 0.20 -1.2
1,377,290 - Pf1N1B4_1216 0.20 -0.0
1,377,299 + Pf1N1B4_1216 0.21 -0.0
1,377,300 - Pf1N1B4_1216 0.21 -0.0
1,377,315 + Pf1N1B4_1216 0.22 -0.0
1,377,316 - Pf1N1B4_1216 0.22 +0.4

Or see this region's nucleotide sequence