Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_11 and Pf1N1B4_12 are separated by 67 nucleotides Pf1N1B4_12 and Pf1N1B4_13 are separated by 93 nucleotides
Pf1N1B4_11 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 12,093 to 13,205
_11
Pf1N1B4_12 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 13,273 to 14,355
_12
Pf1N1B4_13 - SAM-dependent methyltransferase YafE (UbiE paralog), at 14,449 to 15,216
_13
Position (kb) 13
14
15 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 13.223 kb on - strand at 13.223 kb on - strand at 13.298 kb on - strand at 13.378 kb on + strand at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand, within Pf1N1B4_12 at 13.526 kb on + strand at 13.527 kb on - strand, within Pf1N1B4_12 at 13.527 kb on - strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.691 kb on + strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.692 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.881 kb on - strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.934 kb on + strand, within Pf1N1B4_12 at 13.935 kb on - strand, within Pf1N1B4_12 at 14.150 kb on - strand, within Pf1N1B4_12 at 14.261 kb on + strand at 14.261 kb on + strand at 14.262 kb on - strand at 14.422 kb on + strand at 14.481 kb on + strand at 14.513 kb on + strand at 14.513 kb on + strand at 14.606 kb on + strand, within Pf1N1B4_13 at 14.606 kb on + strand, within Pf1N1B4_13 at 14.691 kb on - strand, within Pf1N1B4_13 at 14.691 kb on - strand, within Pf1N1B4_13 at 14.738 kb on + strand, within Pf1N1B4_13 at 14.739 kb on - strand, within Pf1N1B4_13 at 14.972 kb on - strand, within Pf1N1B4_13 at 14.972 kb on - strand, within Pf1N1B4_13 at 15.152 kb on + strand at 15.152 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.184 kb on + strand at 15.185 kb on - strand at 15.187 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 13,223 - -0.0 13,223 - -1.2 13,298 - -2.0 13,378 + -0.0 13,526 + Pf1N1B4_12 0.23 +1.0 13,526 + -4.1 13,526 + Pf1N1B4_12 0.23 -0.0 13,526 + Pf1N1B4_12 0.23 -2.7 13,526 + Pf1N1B4_12 0.23 -4.0 13,526 + Pf1N1B4_12 0.23 -2.9 13,526 + Pf1N1B4_12 0.23 -5.4 13,526 + -5.1 13,527 - Pf1N1B4_12 0.23 -2.7 13,527 - Pf1N1B4_12 0.23 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -5.0 13,691 + -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -0.0 13,691 + Pf1N1B4_12 0.39 -1.7 13,692 - Pf1N1B4_12 0.39 -0.0 13,692 - Pf1N1B4_12 0.39 -0.0 13,692 - Pf1N1B4_12 0.39 -5.1 13,692 - Pf1N1B4_12 0.39 -0.0 13,692 - Pf1N1B4_12 0.39 -0.0 13,881 - Pf1N1B4_12 0.56 -0.0 13,881 - Pf1N1B4_12 0.56 -4.3 13,881 - Pf1N1B4_12 0.56 -0.0 13,934 + Pf1N1B4_12 0.61 -0.0 13,934 + Pf1N1B4_12 0.61 -0.0 13,934 + Pf1N1B4_12 0.61 -4.9 13,935 - Pf1N1B4_12 0.61 -4.9 14,150 - Pf1N1B4_12 0.81 -0.0 14,261 + -1.5 14,261 + -2.8 14,262 - -0.0 14,422 + -3.2 14,481 + -3.4 14,513 + -0.0 14,513 + -3.0 14,606 + Pf1N1B4_13 0.20 -4.4 14,606 + Pf1N1B4_13 0.20 -5.2 14,691 - Pf1N1B4_13 0.32 -0.0 14,691 - Pf1N1B4_13 0.32 -0.0 14,738 + Pf1N1B4_13 0.38 -0.0 14,739 - Pf1N1B4_13 0.38 -0.0 14,972 - Pf1N1B4_13 0.68 -0.0 14,972 - Pf1N1B4_13 0.68 -0.0 15,152 + -0.0 15,152 + -3.7 15,184 + -2.3 15,184 + -1.3 15,184 + -0.0 15,185 - -1.5 15,187 + -2.1
Or see this region's nucleotide sequence