Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1113

Experiment: Nitrate reduction, replicate B t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1111 and Pf1N1B4_1112 are separated by 14 nucleotidesPf1N1B4_1112 and Pf1N1B4_1113 are separated by 139 nucleotidesPf1N1B4_1113 and Pf1N1B4_1114 are separated by 63 nucleotides Pf1N1B4_1111 - Phosphatidylcholine-hydrolyzing phospholipase C (EC 3.1.4.3), at 1,245,644 to 1,246,963 _1111 Pf1N1B4_1112 - hypothetical protein, at 1,246,978 to 1,247,169 _1112 Pf1N1B4_1113 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 1,247,309 to 1,248,184 _1113 Pf1N1B4_1114 - ABC transporter, ATP-binding protein, at 1,248,248 to 1,249,975 _1114 Position (kb) 1247 1248 1249Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1246.337 kb on + strand, within Pf1N1B4_1111at 1246.337 kb on + strand, within Pf1N1B4_1111at 1246.337 kb on + strand, within Pf1N1B4_1111at 1246.337 kb on + strand, within Pf1N1B4_1111at 1246.337 kb on + strand, within Pf1N1B4_1111at 1246.338 kb on - strandat 1246.418 kb on + strand, within Pf1N1B4_1111at 1246.419 kb on - strand, within Pf1N1B4_1111at 1246.458 kb on + strand, within Pf1N1B4_1111at 1246.459 kb on - strand, within Pf1N1B4_1111at 1246.459 kb on - strand, within Pf1N1B4_1111at 1246.459 kb on - strand, within Pf1N1B4_1111at 1246.628 kb on + strand, within Pf1N1B4_1111at 1246.629 kb on - strand, within Pf1N1B4_1111at 1246.709 kb on + strand, within Pf1N1B4_1111at 1246.709 kb on + strand, within Pf1N1B4_1111at 1246.709 kb on + strand, within Pf1N1B4_1111at 1246.710 kb on - strand, within Pf1N1B4_1111at 1246.710 kb on - strand, within Pf1N1B4_1111at 1246.779 kb on + strand, within Pf1N1B4_1111at 1246.779 kb on + strand, within Pf1N1B4_1111at 1246.779 kb on + strand, within Pf1N1B4_1111at 1246.904 kb on + strandat 1246.904 kb on + strandat 1246.905 kb on - strandat 1246.905 kb on - strandat 1246.905 kb on - strandat 1246.922 kb on + strandat 1246.922 kb on + strandat 1246.923 kb on - strandat 1246.983 kb on + strandat 1246.983 kb on + strandat 1246.984 kb on - strandat 1247.175 kb on + strandat 1247.175 kb on + strandat 1247.175 kb on + strandat 1247.175 kb on + strandat 1247.176 kb on - strandat 1247.176 kb on - strandat 1247.231 kb on + strandat 1247.242 kb on + strandat 1247.242 kb on + strandat 1247.428 kb on - strand, within Pf1N1B4_1113at 1247.428 kb on - strand, within Pf1N1B4_1113at 1247.428 kb on - strand, within Pf1N1B4_1113at 1247.428 kb on - strand, within Pf1N1B4_1113at 1247.463 kb on + strand, within Pf1N1B4_1113at 1247.463 kb on + strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.464 kb on - strand, within Pf1N1B4_1113at 1247.557 kb on - strand, within Pf1N1B4_1113at 1247.557 kb on - strand, within Pf1N1B4_1113at 1248.099 kb on + strandat 1248.099 kb on + strandat 1248.099 kb on + strandat 1248.100 kb on - strandat 1248.100 kb on - strandat 1248.115 kb on + strandat 1248.116 kb on - strandat 1248.116 kb on - strandat 1248.432 kb on + strand, within Pf1N1B4_1114at 1248.433 kb on - strand, within Pf1N1B4_1114at 1248.433 kb on - strand, within Pf1N1B4_1114at 1248.433 kb on - strand, within Pf1N1B4_1114at 1248.450 kb on + strand, within Pf1N1B4_1114at 1248.451 kb on - strand, within Pf1N1B4_1114at 1248.577 kb on - strand, within Pf1N1B4_1114at 1248.634 kb on + strand, within Pf1N1B4_1114

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2
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1,246,337 + Pf1N1B4_1111 0.53 -0.0
1,246,337 + Pf1N1B4_1111 0.53 -1.1
1,246,337 + Pf1N1B4_1111 0.53 -1.5
1,246,337 + Pf1N1B4_1111 0.53 -0.0
1,246,337 + Pf1N1B4_1111 0.53 -2.1
1,246,338 - -0.0
1,246,418 + Pf1N1B4_1111 0.59 -1.2
1,246,419 - Pf1N1B4_1111 0.59 -0.0
1,246,458 + Pf1N1B4_1111 0.62 -0.0
1,246,459 - Pf1N1B4_1111 0.62 -1.8
1,246,459 - Pf1N1B4_1111 0.62 -0.0
1,246,459 - Pf1N1B4_1111 0.62 -0.0
1,246,628 + Pf1N1B4_1111 0.75 -0.0
1,246,629 - Pf1N1B4_1111 0.75 -2.5
1,246,709 + Pf1N1B4_1111 0.81 -0.0
1,246,709 + Pf1N1B4_1111 0.81 -0.0
1,246,709 + Pf1N1B4_1111 0.81 -0.0
1,246,710 - Pf1N1B4_1111 0.81 -0.0
1,246,710 - Pf1N1B4_1111 0.81 -0.0
1,246,779 + Pf1N1B4_1111 0.86 -2.1
1,246,779 + Pf1N1B4_1111 0.86 -0.0
1,246,779 + Pf1N1B4_1111 0.86 -0.0
1,246,904 + -0.0
1,246,904 + -0.0
1,246,905 - -0.0
1,246,905 - -0.8
1,246,905 - -0.0
1,246,922 + -0.0
1,246,922 + -0.0
1,246,923 - -3.2
1,246,983 + -2.2
1,246,983 + -1.9
1,246,984 - -0.0
1,247,175 + -1.0
1,247,175 + -0.3
1,247,175 + -0.0
1,247,175 + -1.6
1,247,176 - -0.0
1,247,176 - -1.9
1,247,231 + -2.8
1,247,242 + -3.0
1,247,242 + -0.0
1,247,428 - Pf1N1B4_1113 0.14 -0.0
1,247,428 - Pf1N1B4_1113 0.14 -3.4
1,247,428 - Pf1N1B4_1113 0.14 -3.4
1,247,428 - Pf1N1B4_1113 0.14 -3.1
1,247,463 + Pf1N1B4_1113 0.18 -0.0
1,247,463 + Pf1N1B4_1113 0.18 -1.8
1,247,464 - Pf1N1B4_1113 0.18 -0.0
1,247,464 - Pf1N1B4_1113 0.18 -0.0
1,247,464 - Pf1N1B4_1113 0.18 -3.4
1,247,464 - Pf1N1B4_1113 0.18 -3.2
1,247,464 - Pf1N1B4_1113 0.18 -0.0
1,247,464 - Pf1N1B4_1113 0.18 -3.8
1,247,464 - Pf1N1B4_1113 0.18 -2.9
1,247,464 - Pf1N1B4_1113 0.18 -2.4
1,247,464 - Pf1N1B4_1113 0.18 -5.5
1,247,464 - Pf1N1B4_1113 0.18 -0.0
1,247,464 - Pf1N1B4_1113 0.18 -0.0
1,247,464 - Pf1N1B4_1113 0.18 -0.0
1,247,557 - Pf1N1B4_1113 0.28 -0.0
1,247,557 - Pf1N1B4_1113 0.28 -2.2
1,248,099 + -0.9
1,248,099 + -0.0
1,248,099 + -0.0
1,248,100 - -0.0
1,248,100 - -0.0
1,248,115 + -0.0
1,248,116 - -0.0
1,248,116 - -0.0
1,248,432 + Pf1N1B4_1114 0.11 -1.5
1,248,433 - Pf1N1B4_1114 0.11 -1.9
1,248,433 - Pf1N1B4_1114 0.11 -0.0
1,248,433 - Pf1N1B4_1114 0.11 -0.0
1,248,450 + Pf1N1B4_1114 0.12 -0.0
1,248,451 - Pf1N1B4_1114 0.12 -0.0
1,248,577 - Pf1N1B4_1114 0.19 -0.0
1,248,634 + Pf1N1B4_1114 0.22 -0.0

Or see this region's nucleotide sequence