Experiment: BHIS with Ceftriaxone hemi(heptahydrate) 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4481 and BT4482 are separated by 395 nucleotides BT4482 and BT4483 are separated by 32 nucleotides BT4483 and BT4484 are separated by 43 nucleotides
BT4481: BT4481 - outer membrane protein (NCBI ptt file), at 5,897,634 to 5,898,725
BT4481
BT4482: BT4482 - conserved hypothetical protein (NCBI ptt file), at 5,899,121 to 5,900,389
BT4482
BT4483: BT4483 - conserved hypothetical protein (NCBI ptt file), at 5,900,422 to 5,901,195
BT4483
BT4484: BT4484 - conserved hypothetical protein (NCBI ptt file), at 5,901,239 to 5,901,847
BT4484
Position (kb)
5899
5900
5901 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5898.190 kb on + strand, within BT4481 at 5898.299 kb on + strand, within BT4481 at 5898.300 kb on - strand, within BT4481 at 5898.317 kb on - strand, within BT4481 at 5898.321 kb on + strand, within BT4481 at 5898.340 kb on + strand, within BT4481 at 5898.492 kb on + strand, within BT4481 at 5898.493 kb on - strand, within BT4481 at 5898.493 kb on - strand, within BT4481 at 5898.505 kb on - strand, within BT4481 at 5898.569 kb on + strand, within BT4481 at 5898.570 kb on - strand, within BT4481 at 5898.723 kb on + strand at 5898.749 kb on + strand at 5898.750 kb on - strand at 5898.751 kb on + strand at 5898.751 kb on + strand at 5898.752 kb on - strand at 5898.771 kb on + strand at 5898.783 kb on + strand at 5898.868 kb on + strand at 5898.943 kb on - strand at 5898.990 kb on - strand at 5898.991 kb on + strand at 5898.993 kb on + strand at 5898.993 kb on + strand at 5898.993 kb on + strand at 5898.994 kb on - strand at 5899.094 kb on - strand at 5899.152 kb on + strand at 5899.172 kb on + strand at 5899.180 kb on - strand at 5899.542 kb on - strand, within BT4482 at 5899.543 kb on + strand, within BT4482 at 5899.543 kb on + strand, within BT4482 at 5899.543 kb on + strand, within BT4482 at 5899.544 kb on - strand, within BT4482 at 5899.544 kb on - strand, within BT4482 at 5899.601 kb on + strand, within BT4482 at 5899.679 kb on - strand, within BT4482 at 5899.703 kb on + strand, within BT4482 at 5899.703 kb on + strand, within BT4482 at 5899.801 kb on - strand, within BT4482 at 5899.801 kb on - strand, within BT4482 at 5899.822 kb on + strand, within BT4482 at 5899.853 kb on + strand, within BT4482 at 5899.893 kb on - strand, within BT4482 at 5899.911 kb on - strand, within BT4482 at 5899.916 kb on - strand, within BT4482 at 5900.030 kb on + strand, within BT4482 at 5900.041 kb on - strand, within BT4482 at 5900.052 kb on + strand, within BT4482 at 5900.229 kb on + strand, within BT4482 at 5900.229 kb on + strand, within BT4482 at 5900.250 kb on - strand, within BT4482 at 5900.341 kb on + strand at 5900.388 kb on - strand at 5900.420 kb on + strand at 5900.431 kb on + strand at 5900.431 kb on + strand at 5900.432 kb on - strand at 5900.434 kb on - strand at 5900.437 kb on + strand at 5900.531 kb on + strand, within BT4483 at 5900.682 kb on + strand, within BT4483 at 5900.869 kb on + strand, within BT4483 at 5900.870 kb on - strand, within BT4483 at 5900.870 kb on - strand, within BT4483 at 5900.931 kb on + strand, within BT4483 at 5900.935 kb on + strand, within BT4483 at 5900.936 kb on - strand, within BT4483 at 5900.940 kb on + strand, within BT4483 at 5900.985 kb on + strand, within BT4483 at 5901.114 kb on + strand, within BT4483 at 5901.115 kb on - strand, within BT4483 at 5901.151 kb on - strand at 5901.199 kb on + strand at 5901.250 kb on - strand at 5901.303 kb on + strand, within BT4484 at 5901.355 kb on + strand, within BT4484 at 5901.355 kb on + strand, within BT4484 at 5901.356 kb on - strand, within BT4484 at 5901.357 kb on + strand, within BT4484 at 5901.370 kb on + strand, within BT4484
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Ceftriaxone hemi(heptahydrate) 0.2 mM remove 5,898,190 + BT4481 0.51 +1.6 5,898,299 + BT4481 0.61 -1.0 5,898,300 - BT4481 0.61 -2.1 5,898,317 - BT4481 0.63 -1.0 5,898,321 + BT4481 0.63 +0.0 5,898,340 + BT4481 0.65 -2.3 5,898,492 + BT4481 0.79 -1.0 5,898,493 - BT4481 0.79 -1.3 5,898,493 - BT4481 0.79 -1.3 5,898,505 - BT4481 0.80 -1.6 5,898,569 + BT4481 0.86 +0.0 5,898,570 - BT4481 0.86 -0.6 5,898,723 + -0.4 5,898,749 + +0.0 5,898,750 - -0.1 5,898,751 + -2.0 5,898,751 + -1.6 5,898,752 - -2.3 5,898,771 + -1.4 5,898,783 + -2.7 5,898,868 + -1.2 5,898,943 - +0.0 5,898,990 - -2.5 5,898,991 + -3.2 5,898,993 + -2.2 5,898,993 + +2.0 5,898,993 + +1.6 5,898,994 - +0.0 5,899,094 - -0.5 5,899,152 + -2.0 5,899,172 + -1.0 5,899,180 - +0.0 5,899,542 - BT4482 0.33 +0.0 5,899,543 + BT4482 0.33 -1.9 5,899,543 + BT4482 0.33 -1.2 5,899,543 + BT4482 0.33 -1.6 5,899,544 - BT4482 0.33 -1.3 5,899,544 - BT4482 0.33 -0.4 5,899,601 + BT4482 0.38 +0.0 5,899,679 - BT4482 0.44 -1.7 5,899,703 + BT4482 0.46 +0.6 5,899,703 + BT4482 0.46 -1.2 5,899,801 - BT4482 0.54 +0.0 5,899,801 - BT4482 0.54 -1.0 5,899,822 + BT4482 0.55 +0.7 5,899,853 + BT4482 0.58 +0.0 5,899,893 - BT4482 0.61 -3.2 5,899,911 - BT4482 0.62 -0.3 5,899,916 - BT4482 0.63 -1.0 5,900,030 + BT4482 0.72 -2.4 5,900,041 - BT4482 0.72 -1.6 5,900,052 + BT4482 0.73 -3.0 5,900,229 + BT4482 0.87 -3.3 5,900,229 + BT4482 0.87 -2.4 5,900,250 - BT4482 0.89 -1.3 5,900,341 + -3.2 5,900,388 - +0.0 5,900,420 + +1.0 5,900,431 + +0.0 5,900,431 + -1.6 5,900,432 - +0.0 5,900,434 - -1.9 5,900,437 + +1.0 5,900,531 + BT4483 0.14 +1.0 5,900,682 + BT4483 0.34 +0.3 5,900,869 + BT4483 0.58 +0.0 5,900,870 - BT4483 0.58 +0.4 5,900,870 - BT4483 0.58 -0.5 5,900,931 + BT4483 0.66 -1.0 5,900,935 + BT4483 0.66 -0.1 5,900,936 - BT4483 0.66 -1.0 5,900,940 + BT4483 0.67 -3.2 5,900,985 + BT4483 0.73 -0.5 5,901,114 + BT4483 0.89 -1.0 5,901,115 - BT4483 0.90 +1.0 5,901,151 - -1.6 5,901,199 + -0.6 5,901,250 - -4.0 5,901,303 + BT4484 0.11 -1.6 5,901,355 + BT4484 0.19 -0.6 5,901,355 + BT4484 0.19 -0.7 5,901,356 - BT4484 0.19 +1.6 5,901,357 + BT4484 0.19 -0.4 5,901,370 + BT4484 0.22 +0.0
Or see this region's nucleotide sequence