Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2026

Experiment: BHIS with Ceftriaxone hemi(heptahydrate) 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2024 and BT2025 are separated by 117 nucleotidesBT2025 and BT2026 are separated by 500 nucleotidesBT2026 and BT2027 overlap by 4 nucleotidesBT2027 and BT2028 are separated by 325 nucleotides BT2024: BT2024 - conserved hypothetical protein (NCBI ptt file), at 2,541,785 to 2,542,450 BT2024 BT2025: BT2025 - hypothetical protein (NCBI ptt file), at 2,542,568 to 2,542,930 BT2025 BT2026: BT2026 - endonuclease (NCBI ptt file), at 2,543,431 to 2,544,399 BT2026 BT2027: BT2027 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 2,544,396 to 2,544,833 BT2027 BT2028: BT2028 - conserved hypothetical protein (NCBI ptt file), at 2,545,159 to 2,546,166 BT2028 Position (kb) 2543 2544 2545Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2543.289 kb on - strandat 2543.314 kb on - strandat 2543.337 kb on - strandat 2543.418 kb on + strandat 2543.469 kb on + strandat 2543.551 kb on + strand, within BT2026at 2543.585 kb on - strand, within BT2026at 2543.585 kb on - strand, within BT2026at 2543.716 kb on + strand, within BT2026at 2543.717 kb on - strand, within BT2026at 2543.825 kb on - strand, within BT2026at 2543.868 kb on + strand, within BT2026at 2543.877 kb on + strand, within BT2026at 2543.898 kb on - strand, within BT2026at 2543.981 kb on + strand, within BT2026at 2543.981 kb on + strand, within BT2026at 2543.993 kb on + strand, within BT2026at 2543.993 kb on + strand, within BT2026at 2544.151 kb on - strand, within BT2026at 2544.165 kb on - strand, within BT2026at 2544.209 kb on + strand, within BT2026at 2544.209 kb on + strand, within BT2026at 2544.278 kb on + strand, within BT2026at 2544.278 kb on + strand, within BT2026at 2544.279 kb on - strand, within BT2026at 2544.279 kb on - strand, within BT2026at 2544.352 kb on + strandat 2544.353 kb on - strandat 2544.353 kb on - strandat 2544.354 kb on + strandat 2544.504 kb on + strand, within BT2027at 2544.511 kb on - strand, within BT2027at 2544.511 kb on - strand, within BT2027at 2544.575 kb on + strand, within BT2027at 2544.576 kb on - strand, within BT2027at 2544.609 kb on + strand, within BT2027at 2544.844 kb on + strandat 2544.855 kb on - strandat 2544.906 kb on - strandat 2544.945 kb on - strandat 2545.047 kb on - strandat 2545.047 kb on - strandat 2545.070 kb on + strandat 2545.071 kb on - strandat 2545.137 kb on - strandat 2545.144 kb on + strandat 2545.190 kb on - strandat 2545.316 kb on - strand, within BT2028at 2545.335 kb on + strand, within BT2028

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Ceftriaxone hemi(heptahydrate) 0.2 mM
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2,543,289 - -0.3
2,543,314 - -2.2
2,543,337 - +0.6
2,543,418 + +1.6
2,543,469 + -2.6
2,543,551 + BT2026 0.12 -2.0
2,543,585 - BT2026 0.16 -0.0
2,543,585 - BT2026 0.16 -1.6
2,543,716 + BT2026 0.29 +0.6
2,543,717 - BT2026 0.30 -1.6
2,543,825 - BT2026 0.41 -2.7
2,543,868 + BT2026 0.45 -0.6
2,543,877 + BT2026 0.46 -2.0
2,543,898 - BT2026 0.48 +1.6
2,543,981 + BT2026 0.57 -1.7
2,543,981 + BT2026 0.57 -1.6
2,543,993 + BT2026 0.58 -1.7
2,543,993 + BT2026 0.58 -1.6
2,544,151 - BT2026 0.74 -0.7
2,544,165 - BT2026 0.76 -0.7
2,544,209 + BT2026 0.80 -0.0
2,544,209 + BT2026 0.80 -1.4
2,544,278 + BT2026 0.87 -1.7
2,544,278 + BT2026 0.87 -2.0
2,544,279 - BT2026 0.88 -2.1
2,544,279 - BT2026 0.88 -2.3
2,544,352 + -1.0
2,544,353 - +2.0
2,544,353 - -2.4
2,544,354 + -2.0
2,544,504 + BT2027 0.25 +2.2
2,544,511 - BT2027 0.26 -2.0
2,544,511 - BT2027 0.26 -1.0
2,544,575 + BT2027 0.41 -1.4
2,544,576 - BT2027 0.41 +0.3
2,544,609 + BT2027 0.49 -1.0
2,544,844 + -2.2
2,544,855 - -1.6
2,544,906 - -0.4
2,544,945 - -0.8
2,545,047 - +1.6
2,545,047 - -0.7
2,545,070 + +0.6
2,545,071 - -1.5
2,545,137 - -1.0
2,545,144 + -3.2
2,545,190 - -0.0
2,545,316 - BT2028 0.16 -2.0
2,545,335 + BT2028 0.17 +0.6

Or see this region's nucleotide sequence