Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0004

Experiment: BHIS with Ceftriaxone hemi(heptahydrate) 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0003 and BT0004 are separated by 134 nucleotidesBT0004 and BT0005 are separated by 8 nucleotidesBT0005 and BT0006 are separated by 91 nucleotides BT0003: BT0003 - hypothetical protein (NCBI ptt file), at 1,872 to 2,648 BT0003 BT0004: BT0004 - hypothetical protein (NCBI ptt file), at 2,783 to 3,451 BT0004 BT0005: BT0005 - 30S ribosomal protein S17 (NCBI ptt file), at 3,460 to 3,996 BT0005 BT0006: BT0006 - hypothetical protein (NCBI ptt file), at 4,088 to 4,534 BT0006 Position (kb) 2 3 4Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1.854 kb on + strandat 1.981 kb on + strand, within BT0003at 1.982 kb on - strand, within BT0003at 1.982 kb on - strand, within BT0003at 2.048 kb on - strand, within BT0003at 2.049 kb on + strand, within BT0003at 2.138 kb on - strand, within BT0003at 2.176 kb on - strand, within BT0003at 2.263 kb on - strand, within BT0003at 2.263 kb on - strand, within BT0003at 2.267 kb on - strand, within BT0003at 2.382 kb on - strand, within BT0003at 2.430 kb on - strand, within BT0003at 2.483 kb on - strand, within BT0003at 2.521 kb on - strand, within BT0003at 2.528 kb on + strand, within BT0003at 2.534 kb on + strand, within BT0003at 2.535 kb on - strand, within BT0003at 2.535 kb on - strand, within BT0003at 2.556 kb on - strand, within BT0003at 2.573 kb on + strandat 2.697 kb on - strandat 2.697 kb on - strandat 2.697 kb on - strandat 2.707 kb on - strandat 2.777 kb on - strandat 2.797 kb on - strandat 2.813 kb on - strandat 2.955 kb on - strand, within BT0004at 2.955 kb on - strand, within BT0004at 3.192 kb on + strand, within BT0004at 3.232 kb on + strand, within BT0004at 3.422 kb on - strandat 3.426 kb on - strandat 3.458 kb on - strandat 3.550 kb on - strand, within BT0005at 3.557 kb on + strand, within BT0005at 3.638 kb on - strand, within BT0005at 3.694 kb on - strand, within BT0005at 3.696 kb on + strand, within BT0005at 3.697 kb on - strand, within BT0005at 3.746 kb on + strand, within BT0005at 3.747 kb on - strand, within BT0005at 3.747 kb on - strand, within BT0005at 3.785 kb on + strand, within BT0005at 3.872 kb on - strand, within BT0005at 3.917 kb on + strand, within BT0005at 3.918 kb on - strand, within BT0005at 3.918 kb on - strand, within BT0005at 4.104 kb on + strandat 4.208 kb on - strand, within BT0006at 4.258 kb on - strand, within BT0006at 4.278 kb on - strand, within BT0006at 4.278 kb on - strand, within BT0006at 4.280 kb on - strand, within BT0006at 4.280 kb on - strand, within BT0006at 4.291 kb on - strand, within BT0006at 4.301 kb on - strand, within BT0006at 4.304 kb on + strand, within BT0006at 4.324 kb on + strand, within BT0006at 4.335 kb on - strand, within BT0006at 4.364 kb on - strand, within BT0006at 4.367 kb on + strand, within BT0006

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Ceftriaxone hemi(heptahydrate) 0.2 mM
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1,854 + +0.8
1,981 + BT0003 0.14 +0.0
1,982 - BT0003 0.14 +0.4
1,982 - BT0003 0.14 -1.0
2,048 - BT0003 0.23 +2.3
2,049 + BT0003 0.23 -2.0
2,138 - BT0003 0.34 +1.6
2,176 - BT0003 0.39 +0.0
2,263 - BT0003 0.50 +1.0
2,263 - BT0003 0.50 -1.2
2,267 - BT0003 0.51 +1.0
2,382 - BT0003 0.66 +1.0
2,430 - BT0003 0.72 -2.1
2,483 - BT0003 0.79 -0.4
2,521 - BT0003 0.84 -1.6
2,528 + BT0003 0.84 +0.8
2,534 + BT0003 0.85 -1.0
2,535 - BT0003 0.85 +1.0
2,535 - BT0003 0.85 -1.6
2,556 - BT0003 0.88 -2.3
2,573 + -0.3
2,697 - -2.1
2,697 - -2.4
2,697 - -1.0
2,707 - +0.0
2,777 - -1.5
2,797 - -1.1
2,813 - -1.1
2,955 - BT0004 0.26 -1.0
2,955 - BT0004 0.26 -4.6
3,192 + BT0004 0.61 -1.0
3,232 + BT0004 0.67 -1.6
3,422 - +1.6
3,426 - -1.0
3,458 - -3.1
3,550 - BT0005 0.17 -1.2
3,557 + BT0005 0.18 -2.5
3,638 - BT0005 0.33 -2.6
3,694 - BT0005 0.44 -1.1
3,696 + BT0005 0.44 -2.3
3,697 - BT0005 0.44 -2.9
3,746 + BT0005 0.53 -1.0
3,747 - BT0005 0.53 -1.0
3,747 - BT0005 0.53 +0.4
3,785 + BT0005 0.61 +0.0
3,872 - BT0005 0.77 +1.8
3,917 + BT0005 0.85 -1.7
3,918 - BT0005 0.85 -1.3
3,918 - BT0005 0.85 -1.0
4,104 + -1.8
4,208 - BT0006 0.27 -1.1
4,258 - BT0006 0.38 -1.6
4,278 - BT0006 0.43 -0.7
4,278 - BT0006 0.43 -0.2
4,280 - BT0006 0.43 +0.7
4,280 - BT0006 0.43 -1.3
4,291 - BT0006 0.45 +0.8
4,301 - BT0006 0.48 -1.0
4,304 + BT0006 0.48 +0.0
4,324 + BT0006 0.53 -1.1
4,335 - BT0006 0.55 +0.0
4,364 - BT0006 0.62 +0.6
4,367 + BT0006 0.62 -2.4

Or see this region's nucleotide sequence