Experiment: BG11 with nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt petJ and Synpcc7942_2543 are separated by 16 nucleotides Synpcc7942_2543 and Synpcc7942_2544 are separated by 29 nucleotides Synpcc7942_2544 and aspC are separated by 113 nucleotides
Synpcc7942_2542: petJ - putative cytochrome C6-2, at 2,623,378 to 2,623,725
petJ
Synpcc7942_2543: Synpcc7942_2543 - phage SPO1 DNA polymerase-related protein, at 2,623,742 to 2,624,449
_2543
Synpcc7942_2544: Synpcc7942_2544 - hypothetical protein, at 2,624,479 to 2,624,868
_2544
Synpcc7942_2545: aspC - aspartate aminotransferase, at 2,624,982 to 2,626,160
aspC
Position (kb)
2624
2625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2623.507 kb on + strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.509 kb on + strand, within petJ at 2623.509 kb on + strand, within petJ at 2623.515 kb on - strand, within petJ at 2623.517 kb on - strand, within petJ at 2623.517 kb on - strand, within petJ at 2623.527 kb on - strand, within petJ at 2623.541 kb on + strand, within petJ at 2623.569 kb on + strand, within petJ at 2623.598 kb on + strand, within petJ at 2623.610 kb on + strand, within petJ at 2623.610 kb on + strand at 2623.611 kb on - strand, within petJ at 2623.615 kb on + strand, within petJ at 2623.618 kb on - strand, within petJ at 2623.625 kb on + strand at 2623.633 kb on + strand, within petJ at 2623.671 kb on + strand, within petJ at 2623.694 kb on + strand at 2623.694 kb on - strand at 2623.702 kb on - strand at 2623.714 kb on - strand at 2623.720 kb on + strand at 2623.720 kb on + strand at 2623.720 kb on + strand at 2623.728 kb on - strand at 2623.728 kb on - strand at 2623.728 kb on - strand at 2623.730 kb on + strand at 2623.730 kb on + strand at 2623.738 kb on - strand at 2623.740 kb on + strand at 2623.740 kb on + strand at 2623.747 kb on - strand at 2623.748 kb on - strand at 2623.748 kb on - strand at 2623.822 kb on + strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.845 kb on + strand, within Synpcc7942_2543 at 2623.848 kb on - strand, within Synpcc7942_2543 at 2623.917 kb on + strand, within Synpcc7942_2543 at 2623.925 kb on + strand, within Synpcc7942_2543 at 2623.948 kb on - strand, within Synpcc7942_2543 at 2623.948 kb on - strand, within Synpcc7942_2543 at 2623.984 kb on + strand, within Synpcc7942_2543 at 2623.984 kb on + strand, within Synpcc7942_2543 at 2623.989 kb on - strand, within Synpcc7942_2543 at 2624.043 kb on + strand, within Synpcc7942_2543 at 2624.125 kb on + strand, within Synpcc7942_2543 at 2624.146 kb on - strand, within Synpcc7942_2543 at 2624.171 kb on + strand, within Synpcc7942_2543 at 2624.191 kb on - strand, within Synpcc7942_2543 at 2624.196 kb on - strand, within Synpcc7942_2543 at 2624.224 kb on + strand, within Synpcc7942_2543 at 2624.235 kb on + strand, within Synpcc7942_2543 at 2624.245 kb on + strand, within Synpcc7942_2543 at 2624.247 kb on - strand, within Synpcc7942_2543 at 2624.271 kb on + strand, within Synpcc7942_2543 at 2624.276 kb on + strand, within Synpcc7942_2543 at 2624.286 kb on + strand, within Synpcc7942_2543 at 2624.287 kb on + strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.296 kb on + strand, within Synpcc7942_2543 at 2624.325 kb on - strand, within Synpcc7942_2543 at 2624.346 kb on - strand, within Synpcc7942_2543 at 2624.354 kb on + strand, within Synpcc7942_2543 at 2624.388 kb on - strand at 2624.428 kb on + strand at 2624.428 kb on + strand at 2624.428 kb on + strand at 2624.432 kb on + strand at 2624.451 kb on + strand at 2624.476 kb on + strand at 2624.484 kb on - strand at 2624.484 kb on - strand at 2624.484 kb on - strand at 2624.486 kb on + strand at 2624.494 kb on - strand at 2624.517 kb on + strand at 2624.527 kb on - strand, within Synpcc7942_2544 at 2624.535 kb on - strand, within Synpcc7942_2544 at 2624.572 kb on + strand, within Synpcc7942_2544 at 2624.579 kb on + strand, within Synpcc7942_2544 at 2624.597 kb on + strand, within Synpcc7942_2544 at 2624.617 kb on + strand, within Synpcc7942_2544 at 2624.625 kb on - strand, within Synpcc7942_2544 at 2624.661 kb on + strand, within Synpcc7942_2544 at 2624.661 kb on + strand, within Synpcc7942_2544 at 2624.669 kb on + strand, within Synpcc7942_2544 at 2624.706 kb on + strand, within Synpcc7942_2544 at 2624.706 kb on + strand, within Synpcc7942_2544 at 2624.714 kb on - strand, within Synpcc7942_2544 at 2624.771 kb on + strand, within Synpcc7942_2544 at 2624.771 kb on + strand, within Synpcc7942_2544 at 2624.771 kb on + strand, within Synpcc7942_2544 at 2624.779 kb on - strand, within Synpcc7942_2544 at 2624.779 kb on - strand, within Synpcc7942_2544 at 2624.797 kb on + strand, within Synpcc7942_2544 at 2624.802 kb on + strand, within Synpcc7942_2544 at 2624.802 kb on - strand, within Synpcc7942_2544 at 2624.804 kb on + strand, within Synpcc7942_2544 at 2624.807 kb on + strand, within Synpcc7942_2544 at 2624.807 kb on + strand, within Synpcc7942_2544 at 2624.807 kb on + strand, within Synpcc7942_2544 at 2624.807 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.843 kb on + strand at 2624.853 kb on + strand at 2624.853 kb on + strand at 2624.853 kb on + strand at 2624.861 kb on - strand at 2624.879 kb on + strand at 2624.920 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrate 100 mM remove 2,623,507 + petJ Synpcc7942_2542 0.37 -0.5 2,623,507 + petJ Synpcc7942_2542 0.37 -0.3 2,623,507 + petJ Synpcc7942_2542 0.37 +0.1 2,623,507 + petJ Synpcc7942_2542 0.37 -1.7 2,623,507 + petJ Synpcc7942_2542 0.37 +0.8 2,623,509 + petJ Synpcc7942_2542 0.38 +3.0 2,623,509 + petJ Synpcc7942_2542 0.38 +1.3 2,623,515 - petJ Synpcc7942_2542 0.39 -0.5 2,623,517 - petJ Synpcc7942_2542 0.40 -0.7 2,623,517 - petJ Synpcc7942_2542 0.40 -2.7 2,623,527 - petJ Synpcc7942_2542 0.43 -1.7 2,623,541 + petJ Synpcc7942_2542 0.47 -1.8 2,623,569 + petJ Synpcc7942_2542 0.55 -0.8 2,623,598 + petJ Synpcc7942_2542 0.63 -0.5 2,623,610 + petJ Synpcc7942_2542 0.67 +1.0 2,623,610 + +0.0 2,623,611 - petJ Synpcc7942_2542 0.67 -1.4 2,623,615 + petJ Synpcc7942_2542 0.68 -0.4 2,623,618 - petJ Synpcc7942_2542 0.69 -1.7 2,623,625 + -0.5 2,623,633 + petJ Synpcc7942_2542 0.73 -0.4 2,623,671 + petJ Synpcc7942_2542 0.84 -0.1 2,623,694 + -0.1 2,623,694 - -0.0 2,623,702 - -0.1 2,623,714 - -1.3 2,623,720 + -1.0 2,623,720 + +0.8 2,623,720 + -1.4 2,623,728 - -0.3 2,623,728 - +1.1 2,623,728 - -0.8 2,623,730 + -0.1 2,623,730 + +0.9 2,623,738 - +1.9 2,623,740 + -0.7 2,623,740 + -2.0 2,623,747 - -0.5 2,623,748 - +1.8 2,623,748 - +0.3 2,623,822 + Synpcc7942_2543 0.11 -0.7 2,623,825 - Synpcc7942_2543 0.12 -0.9 2,623,825 - Synpcc7942_2543 0.12 -1.4 2,623,825 - Synpcc7942_2543 0.12 -1.0 2,623,825 - Synpcc7942_2543 0.12 +1.2 2,623,825 - Synpcc7942_2543 0.12 -1.8 2,623,845 + Synpcc7942_2543 0.15 -1.8 2,623,848 - Synpcc7942_2543 0.15 -2.4 2,623,917 + Synpcc7942_2543 0.25 -1.5 2,623,925 + Synpcc7942_2543 0.26 -0.7 2,623,948 - Synpcc7942_2543 0.29 -0.7 2,623,948 - Synpcc7942_2543 0.29 -0.2 2,623,984 + Synpcc7942_2543 0.34 -1.1 2,623,984 + Synpcc7942_2543 0.34 -2.0 2,623,989 - Synpcc7942_2543 0.35 -2.0 2,624,043 + Synpcc7942_2543 0.43 -1.4 2,624,125 + Synpcc7942_2543 0.54 -0.1 2,624,146 - Synpcc7942_2543 0.57 +0.1 2,624,171 + Synpcc7942_2543 0.61 -2.3 2,624,191 - Synpcc7942_2543 0.63 -2.7 2,624,196 - Synpcc7942_2543 0.64 +0.3 2,624,224 + Synpcc7942_2543 0.68 -0.0 2,624,235 + Synpcc7942_2543 0.70 -2.5 2,624,245 + Synpcc7942_2543 0.71 -0.9 2,624,247 - Synpcc7942_2543 0.71 +0.5 2,624,271 + Synpcc7942_2543 0.75 -1.0 2,624,276 + Synpcc7942_2543 0.75 -2.0 2,624,286 + Synpcc7942_2543 0.77 +0.3 2,624,287 + Synpcc7942_2543 0.77 -1.3 2,624,294 - Synpcc7942_2543 0.78 +0.8 2,624,294 - Synpcc7942_2543 0.78 +1.2 2,624,294 - Synpcc7942_2543 0.78 -0.8 2,624,294 - Synpcc7942_2543 0.78 +0.2 2,624,294 - Synpcc7942_2543 0.78 +0.2 2,624,296 + Synpcc7942_2543 0.78 -0.5 2,624,325 - Synpcc7942_2543 0.82 -0.0 2,624,346 - Synpcc7942_2543 0.85 +0.8 2,624,354 + Synpcc7942_2543 0.86 -1.3 2,624,388 - +0.2 2,624,428 + -1.0 2,624,428 + -0.1 2,624,428 + +1.1 2,624,432 + -1.3 2,624,451 + -0.0 2,624,476 + -2.1 2,624,484 - -1.7 2,624,484 - -1.0 2,624,484 - -0.5 2,624,486 + -3.0 2,624,494 - +0.1 2,624,517 + -0.0 2,624,527 - Synpcc7942_2544 0.12 +0.5 2,624,535 - Synpcc7942_2544 0.14 -0.0 2,624,572 + Synpcc7942_2544 0.24 -1.4 2,624,579 + Synpcc7942_2544 0.26 +0.5 2,624,597 + Synpcc7942_2544 0.30 -1.2 2,624,617 + Synpcc7942_2544 0.35 +0.1 2,624,625 - Synpcc7942_2544 0.37 -0.8 2,624,661 + Synpcc7942_2544 0.47 +3.0 2,624,661 + Synpcc7942_2544 0.47 -0.1 2,624,669 + Synpcc7942_2544 0.49 -3.7 2,624,706 + Synpcc7942_2544 0.58 -2.2 2,624,706 + Synpcc7942_2544 0.58 -1.7 2,624,714 - Synpcc7942_2544 0.60 -0.9 2,624,771 + Synpcc7942_2544 0.75 -1.7 2,624,771 + Synpcc7942_2544 0.75 -0.1 2,624,771 + Synpcc7942_2544 0.75 -2.3 2,624,779 - Synpcc7942_2544 0.77 +0.0 2,624,779 - Synpcc7942_2544 0.77 -2.0 2,624,797 + Synpcc7942_2544 0.82 -2.3 2,624,802 + Synpcc7942_2544 0.83 +0.0 2,624,802 - Synpcc7942_2544 0.83 -0.3 2,624,804 + Synpcc7942_2544 0.83 +0.0 2,624,807 + Synpcc7942_2544 0.84 +0.2 2,624,807 + Synpcc7942_2544 0.84 -1.4 2,624,807 + Synpcc7942_2544 0.84 -1.9 2,624,807 - Synpcc7942_2544 0.84 -2.7 2,624,812 - Synpcc7942_2544 0.85 -1.6 2,624,812 - Synpcc7942_2544 0.85 +0.2 2,624,812 - Synpcc7942_2544 0.85 -1.5 2,624,812 - Synpcc7942_2544 0.85 -1.9 2,624,812 - Synpcc7942_2544 0.85 -1.2 2,624,815 - Synpcc7942_2544 0.86 -1.0 2,624,815 - Synpcc7942_2544 0.86 -2.0 2,624,815 - Synpcc7942_2544 0.86 -1.1 2,624,815 - Synpcc7942_2544 0.86 -1.9 2,624,815 - Synpcc7942_2544 0.86 -0.8 2,624,815 - Synpcc7942_2544 0.86 -1.1 2,624,843 + -1.1 2,624,853 + -3.2 2,624,853 + -1.1 2,624,853 + -0.5 2,624,861 - -2.3 2,624,879 + -0.7 2,624,920 - -1.9
Or see this region's nucleotide sequence