Experiment: BG11 with nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sasA and tRNA-Glu are separated by 92 nucleotides tRNA-Glu and Synpcc7942_2115 are separated by 118 nucleotides Synpcc7942_2115 and Synpcc7942_2116 are separated by 161 nucleotides
Synpcc7942_2114: sasA - adaptive-response sensory kinase, at 2,194,827 to 2,195,990
sasA
Synpcc7942_R0007: tRNA-Glu - tRNA-Glu, at 2,196,083 to 2,196,155
tRNA-Glu
Synpcc7942_2115: Synpcc7942_2115 - hypothetical protein, at 2,196,274 to 2,197,128
_2115
Synpcc7942_2116: Synpcc7942_2116 - hypothetical protein, at 2,197,290 to 2,198,150
_2116
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2195.288 kb on + strand, within sasA at 2195.288 kb on + strand, within sasA at 2195.304 kb on + strand, within sasA at 2195.304 kb on + strand, within sasA at 2195.345 kb on + strand, within sasA at 2195.345 kb on + strand, within sasA at 2195.382 kb on - strand, within sasA at 2195.406 kb on + strand, within sasA at 2195.429 kb on + strand, within sasA at 2195.484 kb on + strand, within sasA at 2195.496 kb on + strand, within sasA at 2195.538 kb on - strand, within sasA at 2195.584 kb on - strand, within sasA at 2195.589 kb on - strand, within sasA at 2195.605 kb on - strand, within sasA at 2195.618 kb on - strand, within sasA at 2195.630 kb on + strand, within sasA at 2195.631 kb on + strand, within sasA at 2195.639 kb on - strand, within sasA at 2195.673 kb on + strand, within sasA at 2195.735 kb on - strand, within sasA at 2195.740 kb on - strand, within sasA at 2195.764 kb on - strand, within sasA at 2195.789 kb on - strand, within sasA at 2195.845 kb on + strand, within sasA at 2195.853 kb on + strand, within sasA at 2195.860 kb on + strand, within sasA at 2195.868 kb on - strand, within sasA at 2195.889 kb on + strand at 2195.892 kb on - strand at 2195.917 kb on - strand at 2195.917 kb on - strand at 2195.920 kb on + strand at 2195.931 kb on + strand at 2195.936 kb on + strand at 2195.936 kb on + strand at 2195.939 kb on - strand at 2195.959 kb on - strand at 2195.974 kb on + strand at 2195.995 kb on - strand at 2196.187 kb on - strand at 2196.189 kb on + strand at 2196.210 kb on - strand at 2196.212 kb on + strand at 2196.249 kb on - strand at 2196.270 kb on - strand at 2196.272 kb on + strand at 2196.272 kb on + strand at 2196.272 kb on + strand at 2196.282 kb on + strand at 2196.290 kb on - strand at 2196.299 kb on + strand at 2196.307 kb on - strand at 2196.307 kb on - strand at 2196.361 kb on - strand, within Synpcc7942_2115 at 2196.368 kb on + strand, within Synpcc7942_2115 at 2196.376 kb on - strand, within Synpcc7942_2115 at 2196.378 kb on + strand, within Synpcc7942_2115 at 2196.381 kb on - strand, within Synpcc7942_2115 at 2196.381 kb on - strand, within Synpcc7942_2115 at 2196.381 kb on - strand, within Synpcc7942_2115 at 2196.386 kb on - strand, within Synpcc7942_2115 at 2196.401 kb on + strand, within Synpcc7942_2115 at 2196.401 kb on + strand, within Synpcc7942_2115 at 2196.448 kb on - strand, within Synpcc7942_2115 at 2196.472 kb on - strand, within Synpcc7942_2115 at 2196.485 kb on + strand, within Synpcc7942_2115 at 2196.485 kb on + strand, within Synpcc7942_2115 at 2196.485 kb on + strand, within Synpcc7942_2115 at 2196.493 kb on - strand, within Synpcc7942_2115 at 2196.509 kb on + strand, within Synpcc7942_2115 at 2196.541 kb on + strand, within Synpcc7942_2115 at 2196.544 kb on - strand, within Synpcc7942_2115 at 2196.546 kb on + strand, within Synpcc7942_2115 at 2196.546 kb on + strand, within Synpcc7942_2115 at 2196.546 kb on + strand, within Synpcc7942_2115 at 2196.546 kb on + strand, within Synpcc7942_2115 at 2196.546 kb on + strand, within Synpcc7942_2115 at 2196.549 kb on - strand, within Synpcc7942_2115 at 2196.549 kb on - strand, within Synpcc7942_2115 at 2196.553 kb on + strand, within Synpcc7942_2115 at 2196.553 kb on + strand, within Synpcc7942_2115 at 2196.553 kb on + strand, within Synpcc7942_2115 at 2196.553 kb on + strand, within Synpcc7942_2115 at 2196.562 kb on + strand, within Synpcc7942_2115 at 2196.564 kb on - strand, within Synpcc7942_2115 at 2196.598 kb on + strand, within Synpcc7942_2115 at 2196.598 kb on + strand, within Synpcc7942_2115 at 2196.601 kb on - strand, within Synpcc7942_2115 at 2196.601 kb on - strand, within Synpcc7942_2115 at 2196.661 kb on + strand, within Synpcc7942_2115 at 2196.669 kb on - strand, within Synpcc7942_2115 at 2196.673 kb on + strand, within Synpcc7942_2115 at 2196.733 kb on + strand, within Synpcc7942_2115 at 2196.751 kb on - strand, within Synpcc7942_2115 at 2196.751 kb on - strand, within Synpcc7942_2115 at 2196.753 kb on + strand, within Synpcc7942_2115 at 2196.753 kb on + strand, within Synpcc7942_2115 at 2196.753 kb on + strand, within Synpcc7942_2115 at 2196.753 kb on + strand, within Synpcc7942_2115 at 2196.796 kb on - strand, within Synpcc7942_2115 at 2196.798 kb on + strand, within Synpcc7942_2115 at 2196.804 kb on + strand, within Synpcc7942_2115 at 2196.804 kb on + strand, within Synpcc7942_2115 at 2196.879 kb on - strand, within Synpcc7942_2115 at 2196.895 kb on - strand, within Synpcc7942_2115 at 2196.897 kb on - strand, within Synpcc7942_2115 at 2196.923 kb on - strand, within Synpcc7942_2115 at 2196.938 kb on + strand, within Synpcc7942_2115 at 2196.972 kb on + strand, within Synpcc7942_2115 at 2196.972 kb on + strand, within Synpcc7942_2115 at 2196.980 kb on - strand, within Synpcc7942_2115 at 2196.986 kb on + strand, within Synpcc7942_2115 at 2196.986 kb on + strand, within Synpcc7942_2115 at 2196.986 kb on + strand, within Synpcc7942_2115 at 2196.994 kb on - strand, within Synpcc7942_2115 at 2196.994 kb on - strand, within Synpcc7942_2115 at 2196.994 kb on - strand, within Synpcc7942_2115 at 2196.996 kb on - strand, within Synpcc7942_2115 at 2197.007 kb on + strand, within Synpcc7942_2115 at 2197.007 kb on + strand, within Synpcc7942_2115 at 2197.015 kb on - strand, within Synpcc7942_2115 at 2197.050 kb on - strand at 2197.078 kb on + strand at 2197.109 kb on - strand at 2197.154 kb on - strand at 2197.165 kb on + strand at 2197.173 kb on - strand at 2197.173 kb on - strand at 2197.173 kb on - strand at 2197.187 kb on - strand at 2197.187 kb on - strand at 2197.192 kb on + strand at 2197.209 kb on - strand at 2197.218 kb on + strand at 2197.220 kb on + strand at 2197.228 kb on - strand at 2197.238 kb on - strand at 2197.238 kb on - strand at 2197.260 kb on - strand at 2197.284 kb on + strand at 2197.331 kb on + strand at 2197.334 kb on - strand at 2197.401 kb on + strand, within Synpcc7942_2116 at 2197.401 kb on + strand, within Synpcc7942_2116 at 2197.412 kb on + strand, within Synpcc7942_2116 at 2197.414 kb on - strand, within Synpcc7942_2116 at 2197.423 kb on + strand, within Synpcc7942_2116 at 2197.423 kb on + strand, within Synpcc7942_2116 at 2197.431 kb on - strand, within Synpcc7942_2116 at 2197.431 kb on - strand, within Synpcc7942_2116 at 2197.431 kb on - strand, within Synpcc7942_2116 at 2197.431 kb on - strand, within Synpcc7942_2116 at 2197.436 kb on - strand, within Synpcc7942_2116 at 2197.451 kb on - strand, within Synpcc7942_2116 at 2197.451 kb on - strand, within Synpcc7942_2116 at 2197.451 kb on - strand, within Synpcc7942_2116 at 2197.474 kb on - strand, within Synpcc7942_2116 at 2197.474 kb on - strand, within Synpcc7942_2116 at 2197.481 kb on + strand, within Synpcc7942_2116 at 2197.490 kb on - strand, within Synpcc7942_2116 at 2197.559 kb on + strand, within Synpcc7942_2116 at 2197.562 kb on - strand, within Synpcc7942_2116 at 2197.672 kb on + strand, within Synpcc7942_2116 at 2197.672 kb on + strand, within Synpcc7942_2116 at 2197.680 kb on - strand, within Synpcc7942_2116 at 2197.680 kb on - strand, within Synpcc7942_2116 at 2197.680 kb on - strand, within Synpcc7942_2116 at 2197.680 kb on - strand, within Synpcc7942_2116 at 2197.691 kb on - strand, within Synpcc7942_2116 at 2197.756 kb on + strand, within Synpcc7942_2116 at 2197.763 kb on - strand, within Synpcc7942_2116 at 2197.783 kb on + strand, within Synpcc7942_2116 at 2197.832 kb on + strand, within Synpcc7942_2116 at 2197.834 kb on + strand at 2197.840 kb on - strand, within Synpcc7942_2116 at 2197.870 kb on - strand, within Synpcc7942_2116 at 2197.870 kb on - strand, within Synpcc7942_2116 at 2197.881 kb on + strand, within Synpcc7942_2116 at 2197.883 kb on + strand, within Synpcc7942_2116 at 2197.889 kb on - strand, within Synpcc7942_2116 at 2197.891 kb on - strand, within Synpcc7942_2116 at 2197.899 kb on + strand, within Synpcc7942_2116 at 2197.907 kb on - strand, within Synpcc7942_2116 at 2197.916 kb on - strand, within Synpcc7942_2116 at 2197.945 kb on + strand, within Synpcc7942_2116 at 2197.969 kb on + strand, within Synpcc7942_2116 at 2197.975 kb on - strand, within Synpcc7942_2116 at 2197.977 kb on - strand, within Synpcc7942_2116 at 2197.977 kb on - strand, within Synpcc7942_2116 at 2197.977 kb on - strand, within Synpcc7942_2116 at 2197.982 kb on + strand, within Synpcc7942_2116 at 2197.987 kb on + strand, within Synpcc7942_2116 at 2197.987 kb on + strand, within Synpcc7942_2116 at 2197.987 kb on + strand, within Synpcc7942_2116 at 2197.987 kb on + strand, within Synpcc7942_2116 at 2197.995 kb on - strand, within Synpcc7942_2116 at 2197.995 kb on - strand, within Synpcc7942_2116 at 2197.995 kb on - strand, within Synpcc7942_2116 at 2198.040 kb on - strand, within Synpcc7942_2116 at 2198.040 kb on - strand, within Synpcc7942_2116 at 2198.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrate 100 mM remove 2,195,288 + sasA Synpcc7942_2114 0.40 +0.2 2,195,288 + sasA Synpcc7942_2114 0.40 -1.2 2,195,304 + sasA Synpcc7942_2114 0.41 -1.8 2,195,304 + sasA Synpcc7942_2114 0.41 -3.4 2,195,345 + sasA Synpcc7942_2114 0.45 +0.0 2,195,345 + sasA Synpcc7942_2114 0.45 -3.2 2,195,382 - sasA Synpcc7942_2114 0.48 -1.3 2,195,406 + sasA Synpcc7942_2114 0.50 -0.7 2,195,429 + sasA Synpcc7942_2114 0.52 -1.0 2,195,484 + sasA Synpcc7942_2114 0.56 -3.4 2,195,496 + sasA Synpcc7942_2114 0.57 -1.0 2,195,538 - sasA Synpcc7942_2114 0.61 -0.6 2,195,584 - sasA Synpcc7942_2114 0.65 -3.6 2,195,589 - sasA Synpcc7942_2114 0.65 -0.6 2,195,605 - sasA Synpcc7942_2114 0.67 -3.4 2,195,618 - sasA Synpcc7942_2114 0.68 -1.3 2,195,630 + sasA Synpcc7942_2114 0.69 -1.3 2,195,631 + sasA Synpcc7942_2114 0.69 -0.8 2,195,639 - sasA Synpcc7942_2114 0.70 -3.7 2,195,673 + sasA Synpcc7942_2114 0.73 -2.9 2,195,735 - sasA Synpcc7942_2114 0.78 -1.3 2,195,740 - sasA Synpcc7942_2114 0.78 -2.3 2,195,764 - sasA Synpcc7942_2114 0.80 -1.8 2,195,789 - sasA Synpcc7942_2114 0.83 -4.1 2,195,845 + sasA Synpcc7942_2114 0.87 -1.0 2,195,853 + sasA Synpcc7942_2114 0.88 -2.5 2,195,860 + sasA Synpcc7942_2114 0.89 -4.1 2,195,868 - sasA Synpcc7942_2114 0.89 -0.9 2,195,889 + -4.5 2,195,892 - -2.4 2,195,917 - -1.3 2,195,917 - +1.2 2,195,920 + +0.4 2,195,931 + -0.3 2,195,936 + -1.2 2,195,936 + -0.3 2,195,939 - -1.2 2,195,959 - -0.6 2,195,974 + -0.1 2,195,995 - -0.6 2,196,187 - -0.7 2,196,189 + -1.8 2,196,210 - -0.8 2,196,212 + -1.1 2,196,249 - -1.7 2,196,270 - +0.0 2,196,272 + +0.9 2,196,272 + -1.5 2,196,272 + -2.0 2,196,282 + +1.6 2,196,290 - -0.0 2,196,299 + +0.5 2,196,307 - +0.2 2,196,307 - +0.1 2,196,361 - Synpcc7942_2115 0.10 -0.9 2,196,368 + Synpcc7942_2115 0.11 -1.5 2,196,376 - Synpcc7942_2115 0.12 +0.9 2,196,378 + Synpcc7942_2115 0.12 +1.1 2,196,381 - Synpcc7942_2115 0.13 +0.7 2,196,381 - Synpcc7942_2115 0.13 +1.8 2,196,381 - Synpcc7942_2115 0.13 -0.0 2,196,386 - Synpcc7942_2115 0.13 -0.9 2,196,401 + Synpcc7942_2115 0.15 +1.2 2,196,401 + Synpcc7942_2115 0.15 -1.0 2,196,448 - Synpcc7942_2115 0.20 -0.1 2,196,472 - Synpcc7942_2115 0.23 +0.1 2,196,485 + Synpcc7942_2115 0.25 +0.2 2,196,485 + Synpcc7942_2115 0.25 -0.5 2,196,485 + Synpcc7942_2115 0.25 -0.2 2,196,493 - Synpcc7942_2115 0.26 +0.9 2,196,509 + Synpcc7942_2115 0.27 -3.3 2,196,541 + Synpcc7942_2115 0.31 -1.5 2,196,544 - Synpcc7942_2115 0.32 -0.1 2,196,546 + Synpcc7942_2115 0.32 -0.5 2,196,546 + Synpcc7942_2115 0.32 -0.7 2,196,546 + Synpcc7942_2115 0.32 -0.4 2,196,546 + Synpcc7942_2115 0.32 -0.4 2,196,546 + Synpcc7942_2115 0.32 -2.0 2,196,549 - Synpcc7942_2115 0.32 +1.0 2,196,549 - Synpcc7942_2115 0.32 -1.4 2,196,553 + Synpcc7942_2115 0.33 +2.2 2,196,553 + Synpcc7942_2115 0.33 -0.3 2,196,553 + Synpcc7942_2115 0.33 -0.4 2,196,553 + Synpcc7942_2115 0.33 +1.0 2,196,562 + Synpcc7942_2115 0.34 -0.1 2,196,564 - Synpcc7942_2115 0.34 -0.3 2,196,598 + Synpcc7942_2115 0.38 +1.5 2,196,598 + Synpcc7942_2115 0.38 +0.6 2,196,601 - Synpcc7942_2115 0.38 -0.5 2,196,601 - Synpcc7942_2115 0.38 -0.3 2,196,661 + Synpcc7942_2115 0.45 -0.4 2,196,669 - Synpcc7942_2115 0.46 +0.0 2,196,673 + Synpcc7942_2115 0.47 +0.7 2,196,733 + Synpcc7942_2115 0.54 -0.0 2,196,751 - Synpcc7942_2115 0.56 -1.2 2,196,751 - Synpcc7942_2115 0.56 +0.2 2,196,753 + Synpcc7942_2115 0.56 +0.6 2,196,753 + Synpcc7942_2115 0.56 -0.1 2,196,753 + Synpcc7942_2115 0.56 +0.0 2,196,753 + Synpcc7942_2115 0.56 +0.4 2,196,796 - Synpcc7942_2115 0.61 -1.6 2,196,798 + Synpcc7942_2115 0.61 -1.7 2,196,804 + Synpcc7942_2115 0.62 -2.5 2,196,804 + Synpcc7942_2115 0.62 +0.1 2,196,879 - Synpcc7942_2115 0.71 -0.8 2,196,895 - Synpcc7942_2115 0.73 -1.8 2,196,897 - Synpcc7942_2115 0.73 +0.7 2,196,923 - Synpcc7942_2115 0.76 -0.4 2,196,938 + Synpcc7942_2115 0.78 +1.8 2,196,972 + Synpcc7942_2115 0.82 -0.1 2,196,972 + Synpcc7942_2115 0.82 +0.7 2,196,980 - Synpcc7942_2115 0.83 +1.4 2,196,986 + Synpcc7942_2115 0.83 -0.8 2,196,986 + Synpcc7942_2115 0.83 -1.1 2,196,986 + Synpcc7942_2115 0.83 -0.0 2,196,994 - Synpcc7942_2115 0.84 -2.6 2,196,994 - Synpcc7942_2115 0.84 +0.3 2,196,994 - Synpcc7942_2115 0.84 -0.1 2,196,996 - Synpcc7942_2115 0.84 -0.5 2,197,007 + Synpcc7942_2115 0.86 +0.5 2,197,007 + Synpcc7942_2115 0.86 +0.4 2,197,015 - Synpcc7942_2115 0.87 -2.0 2,197,050 - +1.2 2,197,078 + -0.6 2,197,109 - -0.5 2,197,154 - +1.2 2,197,165 + +0.3 2,197,173 - +0.1 2,197,173 - -1.6 2,197,173 - -1.3 2,197,187 - +0.4 2,197,187 - +1.7 2,197,192 + -0.5 2,197,209 - +0.4 2,197,218 + +0.1 2,197,220 + +0.5 2,197,228 - -0.6 2,197,238 - +0.2 2,197,238 - -1.1 2,197,260 - -1.2 2,197,284 + -0.6 2,197,331 + -0.1 2,197,334 - +2.0 2,197,401 + Synpcc7942_2116 0.13 -2.9 2,197,401 + Synpcc7942_2116 0.13 -1.8 2,197,412 + Synpcc7942_2116 0.14 +0.4 2,197,414 - Synpcc7942_2116 0.14 -0.3 2,197,423 + Synpcc7942_2116 0.15 -0.4 2,197,423 + Synpcc7942_2116 0.15 +0.1 2,197,431 - Synpcc7942_2116 0.16 -1.9 2,197,431 - Synpcc7942_2116 0.16 -1.6 2,197,431 - Synpcc7942_2116 0.16 -0.6 2,197,431 - Synpcc7942_2116 0.16 -0.4 2,197,436 - Synpcc7942_2116 0.17 +0.8 2,197,451 - Synpcc7942_2116 0.19 -0.3 2,197,451 - Synpcc7942_2116 0.19 +1.8 2,197,451 - Synpcc7942_2116 0.19 -0.6 2,197,474 - Synpcc7942_2116 0.21 +0.0 2,197,474 - Synpcc7942_2116 0.21 -0.7 2,197,481 + Synpcc7942_2116 0.22 -0.2 2,197,490 - Synpcc7942_2116 0.23 +1.1 2,197,559 + Synpcc7942_2116 0.31 -0.8 2,197,562 - Synpcc7942_2116 0.32 +0.8 2,197,672 + Synpcc7942_2116 0.44 -0.6 2,197,672 + Synpcc7942_2116 0.44 +0.7 2,197,680 - Synpcc7942_2116 0.45 +0.2 2,197,680 - Synpcc7942_2116 0.45 -0.0 2,197,680 - Synpcc7942_2116 0.45 +0.4 2,197,680 - Synpcc7942_2116 0.45 +0.0 2,197,691 - Synpcc7942_2116 0.47 +1.2 2,197,756 + Synpcc7942_2116 0.54 -1.5 2,197,763 - Synpcc7942_2116 0.55 +2.1 2,197,783 + Synpcc7942_2116 0.57 -1.5 2,197,832 + Synpcc7942_2116 0.63 -0.1 2,197,834 + -0.8 2,197,840 - Synpcc7942_2116 0.64 -1.4 2,197,870 - Synpcc7942_2116 0.67 -0.1 2,197,870 - Synpcc7942_2116 0.67 -0.4 2,197,881 + Synpcc7942_2116 0.69 -0.6 2,197,883 + Synpcc7942_2116 0.69 -0.6 2,197,889 - Synpcc7942_2116 0.70 +0.1 2,197,891 - Synpcc7942_2116 0.70 +0.8 2,197,899 + Synpcc7942_2116 0.71 +0.2 2,197,907 - Synpcc7942_2116 0.72 -0.3 2,197,916 - Synpcc7942_2116 0.73 +0.6 2,197,945 + Synpcc7942_2116 0.76 -1.5 2,197,969 + Synpcc7942_2116 0.79 +1.4 2,197,975 - Synpcc7942_2116 0.80 -0.4 2,197,977 - Synpcc7942_2116 0.80 -1.6 2,197,977 - Synpcc7942_2116 0.80 -1.5 2,197,977 - Synpcc7942_2116 0.80 -0.7 2,197,982 + Synpcc7942_2116 0.80 -0.3 2,197,987 + Synpcc7942_2116 0.81 -0.9 2,197,987 + Synpcc7942_2116 0.81 -1.7 2,197,987 + Synpcc7942_2116 0.81 -1.2 2,197,987 + Synpcc7942_2116 0.81 +0.7 2,197,995 - Synpcc7942_2116 0.82 -0.8 2,197,995 - Synpcc7942_2116 0.82 -0.3 2,197,995 - Synpcc7942_2116 0.82 -2.3 2,198,040 - Synpcc7942_2116 0.87 +1.0 2,198,040 - Synpcc7942_2116 0.87 -1.4 2,198,077 - +1.2
Or see this region's nucleotide sequence