Experiment: Nitrate reduction, replicate B t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5250 and Pf1N1B4_5251 are separated by 522 nucleotides Pf1N1B4_5251 and Pf1N1B4_5252 are separated by 169 nucleotides Pf1N1B4_5252 and Pf1N1B4_5253 are separated by 11 nucleotides
Pf1N1B4_5250 - hypothetical protein, at 8,866 to 8,994
_5250
Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368
_5251
Pf1N1B4_5252 - hypothetical protein, at 10,538 to 11,272
_5252
Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045
_5253
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 8.594 kb on + strand at 8.595 kb on - strand at 8.595 kb on - strand at 8.642 kb on + strand at 8.643 kb on - strand at 8.654 kb on + strand at 8.655 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.676 kb on - strand at 8.761 kb on + strand at 8.761 kb on + strand at 8.814 kb on + strand at 8.818 kb on + strand at 8.819 kb on - strand at 8.819 kb on - strand at 9.008 kb on - strand at 9.074 kb on + strand at 9.074 kb on + strand at 9.075 kb on - strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.410 kb on + strand at 9.410 kb on + strand at 9.411 kb on - strand at 9.411 kb on - strand at 9.417 kb on + strand at 9.417 kb on + strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.422 kb on - strand at 9.422 kb on - strand at 9.518 kb on + strand at 9.518 kb on + strand at 9.519 kb on - strand at 9.558 kb on + strand at 9.558 kb on + strand at 9.596 kb on + strand at 9.597 kb on - strand at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.640 kb on - strand, within Pf1N1B4_5251 at 9.712 kb on + strand, within Pf1N1B4_5251 at 9.920 kb on + strand at 9.920 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.136 kb on + strand, within Pf1N1B4_5251 at 10.137 kb on - strand, within Pf1N1B4_5251 at 10.137 kb on - strand, within Pf1N1B4_5251 at 10.137 kb on - strand at 10.137 kb on - strand at 10.357 kb on - strand at 10.384 kb on + strand at 10.401 kb on - strand at 10.401 kb on - strand at 10.453 kb on - strand at 10.453 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.552 kb on - strand at 10.733 kb on + strand, within Pf1N1B4_5252 at 10.780 kb on - strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.819 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.841 kb on - strand, within Pf1N1B4_5252 at 10.929 kb on + strand, within Pf1N1B4_5252 at 11.130 kb on + strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.159 kb on + strand, within Pf1N1B4_5252 at 11.205 kb on + strand at 11.206 kb on - strand at 11.206 kb on - strand at 11.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1 remove 8,594 + -0.3 8,595 - -0.0 8,595 - -0.0 8,642 + -0.0 8,643 - -2.1 8,654 + -0.0 8,655 - -0.0 8,659 - -0.0 8,659 - -0.0 8,659 - -0.0 8,659 - -0.0 8,659 - -0.0 8,676 - -0.0 8,761 + -0.0 8,761 + -0.0 8,814 + -0.0 8,818 + -0.0 8,819 - -2.8 8,819 - -0.8 9,008 - -2.2 9,074 + -1.9 9,074 + -1.8 9,075 - -0.0 9,406 + -0.0 9,406 + -0.5 9,406 + -0.0 9,406 + -0.0 9,406 + -1.7 9,407 - -0.2 9,407 - -0.0 9,407 - -0.0 9,407 - -0.0 9,410 + -0.0 9,410 + -1.0 9,411 - -2.0 9,411 - -1.0 9,417 + -1.9 9,417 + -1.4 9,418 - -1.6 9,418 - -0.0 9,418 - -2.3 9,418 - -0.0 9,418 - -0.0 9,418 - -2.0 9,418 - -0.0 9,418 - -0.0 9,418 - -2.3 9,418 - -0.0 9,418 - -1.5 9,422 - -1.9 9,422 - -2.3 9,518 + -0.0 9,518 + -0.0 9,519 - -0.0 9,558 + -0.0 9,558 + -3.3 9,596 + -0.4 9,597 - -0.0 9,640 - Pf1N1B4_5251 0.14 -2.2 9,640 - Pf1N1B4_5251 0.14 -2.2 9,640 - Pf1N1B4_5251 0.14 -2.2 9,640 - Pf1N1B4_5251 0.14 -1.9 9,640 - Pf1N1B4_5251 0.14 -0.0 9,640 - Pf1N1B4_5251 0.14 -2.1 9,712 + Pf1N1B4_5251 0.23 -1.8 9,920 + -0.0 9,920 + Pf1N1B4_5251 0.47 -0.0 10,136 + Pf1N1B4_5251 0.73 -0.0 10,136 + Pf1N1B4_5251 0.73 -2.0 10,136 + Pf1N1B4_5251 0.73 -2.4 10,136 + Pf1N1B4_5251 0.73 -0.0 10,136 + Pf1N1B4_5251 0.73 -1.2 10,136 + Pf1N1B4_5251 0.73 -0.0 10,136 + Pf1N1B4_5251 0.73 -0.0 10,136 + Pf1N1B4_5251 0.73 -0.0 10,137 - Pf1N1B4_5251 0.73 -2.7 10,137 - Pf1N1B4_5251 0.73 -1.0 10,137 - -0.0 10,137 - -3.9 10,357 - -1.2 10,384 + -1.6 10,401 - -0.0 10,401 - -0.8 10,453 - -0.4 10,453 - -1.9 10,496 - -0.0 10,496 - -0.3 10,496 - -1.2 10,496 - -2.2 10,500 - -2.5 10,500 - -1.0 10,500 - -3.1 10,500 - -0.0 10,552 - -3.2 10,733 + Pf1N1B4_5252 0.27 -1.3 10,780 - Pf1N1B4_5252 0.33 -1.1 10,803 + Pf1N1B4_5252 0.36 -0.0 10,803 + Pf1N1B4_5252 0.36 -0.0 10,819 + Pf1N1B4_5252 0.38 -0.0 10,821 + Pf1N1B4_5252 0.39 -1.9 10,821 + Pf1N1B4_5252 0.39 -0.0 10,821 + Pf1N1B4_5252 0.39 -1.6 10,822 - Pf1N1B4_5252 0.39 +0.2 10,822 - Pf1N1B4_5252 0.39 -1.5 10,822 - Pf1N1B4_5252 0.39 -0.0 10,841 - Pf1N1B4_5252 0.41 -0.0 10,929 + Pf1N1B4_5252 0.53 -0.0 11,130 + Pf1N1B4_5252 0.81 -1.1 11,131 - Pf1N1B4_5252 0.81 -0.8 11,131 - Pf1N1B4_5252 0.81 -0.0 11,131 - Pf1N1B4_5252 0.81 -1.1 11,134 + Pf1N1B4_5252 0.81 -0.0 11,134 + Pf1N1B4_5252 0.81 -0.0 11,159 + Pf1N1B4_5252 0.84 -1.3 11,205 + -0.6 11,206 - -0.0 11,206 - -0.0 11,206 - -1.8
Or see this region's nucleotide sequence