Experiment: Nitrate reduction, replicate B t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4643 and Pf1N1B4_4644 are separated by 370 nucleotides Pf1N1B4_4644 and Pf1N1B4_4645 are separated by 245 nucleotides Pf1N1B4_4645 and Pf1N1B4_4646 are separated by 123 nucleotides
Pf1N1B4_4643 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form, at 5,118,967 to 5,123,874
_4643
Pf1N1B4_4644 - hypothetical protein, at 5,124,245 to 5,124,505
_4644
Pf1N1B4_4645 - FIG00954888: hypothetical protein, at 5,124,751 to 5,125,044
_4645
Pf1N1B4_4646 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 5,125,168 to 5,126,601
_4646
Position (kb)
5124
5125 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.356 kb on + strand, within Pf1N1B4_4643 at 5123.416 kb on + strand at 5123.416 kb on + strand at 5123.416 kb on + strand at 5123.417 kb on - strand at 5123.485 kb on + strand at 5123.485 kb on + strand at 5123.767 kb on + strand at 5123.767 kb on + strand at 5123.767 kb on + strand at 5124.077 kb on + strand at 5124.078 kb on - strand at 5124.210 kb on + strand at 5124.211 kb on - strand at 5124.217 kb on - strand at 5124.232 kb on - strand at 5124.247 kb on - strand at 5124.285 kb on + strand at 5124.285 kb on + strand, within Pf1N1B4_4644 at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand, within Pf1N1B4_4644 at 5124.286 kb on - strand at 5124.545 kb on + strand at 5124.545 kb on + strand at 5124.546 kb on - strand at 5124.611 kb on + strand at 5124.611 kb on + strand at 5124.924 kb on - strand, within Pf1N1B4_4645 at 5124.924 kb on - strand, within Pf1N1B4_4645 at 5124.924 kb on - strand, within Pf1N1B4_4645 at 5125.148 kb on + strand at 5125.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1 remove 5,123,356 + Pf1N1B4_4643 0.89 -1.4 5,123,356 + Pf1N1B4_4643 0.89 +0.0 5,123,356 + Pf1N1B4_4643 0.89 -2.7 5,123,356 + Pf1N1B4_4643 0.89 +0.0 5,123,356 + Pf1N1B4_4643 0.89 +0.0 5,123,356 + Pf1N1B4_4643 0.89 -1.0 5,123,416 + +0.0 5,123,416 + +0.0 5,123,416 + -2.0 5,123,417 - -1.5 5,123,485 + -1.9 5,123,485 + +0.0 5,123,767 + +0.0 5,123,767 + +0.0 5,123,767 + +0.0 5,124,077 + -1.0 5,124,078 - -1.7 5,124,210 + -2.7 5,124,211 - +0.0 5,124,217 - -1.7 5,124,232 - -1.3 5,124,247 - +0.0 5,124,285 + +0.0 5,124,285 + Pf1N1B4_4644 0.15 -3.2 5,124,286 - Pf1N1B4_4644 0.16 +0.0 5,124,286 - +0.0 5,124,286 - Pf1N1B4_4644 0.16 +0.0 5,124,286 - +0.0 5,124,286 - Pf1N1B4_4644 0.16 -2.1 5,124,286 - Pf1N1B4_4644 0.16 -2.4 5,124,286 - -2.1 5,124,286 - Pf1N1B4_4644 0.16 -1.8 5,124,286 - Pf1N1B4_4644 0.16 -1.3 5,124,286 - +0.0 5,124,545 + -0.4 5,124,545 + +0.0 5,124,546 - -2.0 5,124,611 + -0.8 5,124,611 + -1.3 5,124,924 - Pf1N1B4_4645 0.59 +0.0 5,124,924 - Pf1N1B4_4645 0.59 +0.0 5,124,924 - Pf1N1B4_4645 0.59 +0.0 5,125,148 + -1.4 5,125,299 - -0.2
Or see this region's nucleotide sequence