Experiment: Nitrate reduction, replicate B t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4479 and Pf1N1B4_4480 are separated by 1 nucleotides Pf1N1B4_4480 and Pf1N1B4_4481 are separated by 185 nucleotides Pf1N1B4_4481 and Pf1N1B4_4482 are separated by 128 nucleotides
Pf1N1B4_4479 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 4,940,451 to 4,941,509
_4479
Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746
_4480
Pf1N1B4_4481 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 4,942,932 to 4,943,420
_4481
Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412
_4482
Position (kb)
4941
4942
4943 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4940.575 kb on + strand, within Pf1N1B4_4479 at 4940.773 kb on + strand, within Pf1N1B4_4479 at 4940.774 kb on - strand at 4940.774 kb on - strand, within Pf1N1B4_4479 at 4940.774 kb on - strand, within Pf1N1B4_4479 at 4940.788 kb on + strand, within Pf1N1B4_4479 at 4940.789 kb on - strand, within Pf1N1B4_4479 at 4940.851 kb on + strand, within Pf1N1B4_4479 at 4940.852 kb on - strand, within Pf1N1B4_4479 at 4940.852 kb on - strand, within Pf1N1B4_4479 at 4940.935 kb on + strand, within Pf1N1B4_4479 at 4940.935 kb on + strand, within Pf1N1B4_4479 at 4941.010 kb on + strand, within Pf1N1B4_4479 at 4941.095 kb on - strand, within Pf1N1B4_4479 at 4941.209 kb on - strand, within Pf1N1B4_4479 at 4941.323 kb on - strand, within Pf1N1B4_4479 at 4941.367 kb on + strand, within Pf1N1B4_4479 at 4941.563 kb on + strand at 4941.782 kb on + strand, within Pf1N1B4_4480 at 4941.970 kb on - strand, within Pf1N1B4_4480 at 4942.235 kb on + strand, within Pf1N1B4_4480 at 4942.427 kb on + strand, within Pf1N1B4_4480 at 4942.668 kb on - strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.779 kb on - strand at 4942.779 kb on - strand at 4942.851 kb on - strand at 4942.904 kb on - strand at 4943.226 kb on + strand, within Pf1N1B4_4481 at 4943.251 kb on - strand, within Pf1N1B4_4481 at 4943.251 kb on - strand at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.550 kb on + strand at 4943.550 kb on + strand at 4943.578 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1 remove 4,940,575 + Pf1N1B4_4479 0.12 -1.8 4,940,773 + Pf1N1B4_4479 0.30 +0.0 4,940,774 - -1.0 4,940,774 - Pf1N1B4_4479 0.31 +0.0 4,940,774 - Pf1N1B4_4479 0.31 +0.0 4,940,788 + Pf1N1B4_4479 0.32 +0.0 4,940,789 - Pf1N1B4_4479 0.32 -2.3 4,940,851 + Pf1N1B4_4479 0.38 -1.0 4,940,852 - Pf1N1B4_4479 0.38 +0.0 4,940,852 - Pf1N1B4_4479 0.38 +0.0 4,940,935 + Pf1N1B4_4479 0.46 +0.0 4,940,935 + Pf1N1B4_4479 0.46 +0.0 4,941,010 + Pf1N1B4_4479 0.53 +0.0 4,941,095 - Pf1N1B4_4479 0.61 +0.0 4,941,209 - Pf1N1B4_4479 0.72 -0.9 4,941,323 - Pf1N1B4_4479 0.82 +0.0 4,941,367 + Pf1N1B4_4479 0.86 +0.0 4,941,563 + +0.0 4,941,782 + Pf1N1B4_4480 0.22 +0.0 4,941,970 - Pf1N1B4_4480 0.37 +0.0 4,942,235 + Pf1N1B4_4480 0.59 -2.0 4,942,427 + Pf1N1B4_4480 0.74 -1.6 4,942,668 - +0.0 4,942,753 + +0.0 4,942,753 + +0.0 4,942,753 + -3.3 4,942,779 - -2.0 4,942,779 - +0.0 4,942,851 - +0.0 4,942,904 - +0.0 4,943,226 + Pf1N1B4_4481 0.60 -2.8 4,943,251 - Pf1N1B4_4481 0.65 +0.0 4,943,251 - -2.0 4,943,370 + Pf1N1B4_4481 0.90 +0.0 4,943,370 + Pf1N1B4_4481 0.90 -1.9 4,943,370 + Pf1N1B4_4481 0.90 -3.3 4,943,372 + +0.1 4,943,372 + -1.5 4,943,372 + -1.6 4,943,372 + +0.0 4,943,550 + +0.0 4,943,550 + +0.0 4,943,578 - -2.3
Or see this region's nucleotide sequence