Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4480

Experiment: Nitrate reduction, replicate B t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4479 and Pf1N1B4_4480 are separated by 1 nucleotidesPf1N1B4_4480 and Pf1N1B4_4481 are separated by 185 nucleotidesPf1N1B4_4481 and Pf1N1B4_4482 are separated by 128 nucleotides Pf1N1B4_4479 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 4,940,451 to 4,941,509 _4479 Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746 _4480 Pf1N1B4_4481 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 4,942,932 to 4,943,420 _4481 Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412 _4482 Position (kb) 4941 4942 4943Strain fitness (log2 ratio) -3 -2 -1 0 1at 4940.575 kb on + strand, within Pf1N1B4_4479at 4940.773 kb on + strand, within Pf1N1B4_4479at 4940.774 kb on - strandat 4940.774 kb on - strand, within Pf1N1B4_4479at 4940.774 kb on - strand, within Pf1N1B4_4479at 4940.788 kb on + strand, within Pf1N1B4_4479at 4940.789 kb on - strand, within Pf1N1B4_4479at 4940.851 kb on + strand, within Pf1N1B4_4479at 4940.852 kb on - strand, within Pf1N1B4_4479at 4940.852 kb on - strand, within Pf1N1B4_4479at 4940.935 kb on + strand, within Pf1N1B4_4479at 4940.935 kb on + strand, within Pf1N1B4_4479at 4941.010 kb on + strand, within Pf1N1B4_4479at 4941.095 kb on - strand, within Pf1N1B4_4479at 4941.209 kb on - strand, within Pf1N1B4_4479at 4941.323 kb on - strand, within Pf1N1B4_4479at 4941.367 kb on + strand, within Pf1N1B4_4479at 4941.563 kb on + strandat 4941.782 kb on + strand, within Pf1N1B4_4480at 4941.970 kb on - strand, within Pf1N1B4_4480at 4942.235 kb on + strand, within Pf1N1B4_4480at 4942.427 kb on + strand, within Pf1N1B4_4480at 4942.668 kb on - strandat 4942.753 kb on + strandat 4942.753 kb on + strandat 4942.753 kb on + strandat 4942.779 kb on - strandat 4942.779 kb on - strandat 4942.851 kb on - strandat 4942.904 kb on - strandat 4943.226 kb on + strand, within Pf1N1B4_4481at 4943.251 kb on - strand, within Pf1N1B4_4481at 4943.251 kb on - strandat 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.550 kb on + strandat 4943.550 kb on + strandat 4943.578 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1
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4,940,575 + Pf1N1B4_4479 0.12 -1.8
4,940,773 + Pf1N1B4_4479 0.30 +0.0
4,940,774 - -1.0
4,940,774 - Pf1N1B4_4479 0.31 +0.0
4,940,774 - Pf1N1B4_4479 0.31 +0.0
4,940,788 + Pf1N1B4_4479 0.32 +0.0
4,940,789 - Pf1N1B4_4479 0.32 -2.3
4,940,851 + Pf1N1B4_4479 0.38 -1.0
4,940,852 - Pf1N1B4_4479 0.38 +0.0
4,940,852 - Pf1N1B4_4479 0.38 +0.0
4,940,935 + Pf1N1B4_4479 0.46 +0.0
4,940,935 + Pf1N1B4_4479 0.46 +0.0
4,941,010 + Pf1N1B4_4479 0.53 +0.0
4,941,095 - Pf1N1B4_4479 0.61 +0.0
4,941,209 - Pf1N1B4_4479 0.72 -0.9
4,941,323 - Pf1N1B4_4479 0.82 +0.0
4,941,367 + Pf1N1B4_4479 0.86 +0.0
4,941,563 + +0.0
4,941,782 + Pf1N1B4_4480 0.22 +0.0
4,941,970 - Pf1N1B4_4480 0.37 +0.0
4,942,235 + Pf1N1B4_4480 0.59 -2.0
4,942,427 + Pf1N1B4_4480 0.74 -1.6
4,942,668 - +0.0
4,942,753 + +0.0
4,942,753 + +0.0
4,942,753 + -3.3
4,942,779 - -2.0
4,942,779 - +0.0
4,942,851 - +0.0
4,942,904 - +0.0
4,943,226 + Pf1N1B4_4481 0.60 -2.8
4,943,251 - Pf1N1B4_4481 0.65 +0.0
4,943,251 - -2.0
4,943,370 + Pf1N1B4_4481 0.90 +0.0
4,943,370 + Pf1N1B4_4481 0.90 -1.9
4,943,370 + Pf1N1B4_4481 0.90 -3.3
4,943,372 + +0.1
4,943,372 + -1.5
4,943,372 + -1.6
4,943,372 + +0.0
4,943,550 + +0.0
4,943,550 + +0.0
4,943,578 - -2.3

Or see this region's nucleotide sequence