Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_43

Experiment: Nitrate reduction, replicate B t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_42 and Pf1N1B4_43 are separated by 327 nucleotidesPf1N1B4_43 and Pf1N1B4_44 are separated by 145 nucleotides Pf1N1B4_42 - hypothetical protein, at 49,101 to 50,606 _42 Pf1N1B4_43 - hypothetical protein, at 50,934 to 51,638 _43 Pf1N1B4_44 - Multicopper oxidase, at 51,784 to 53,577 _44 Position (kb) 50 51 52Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 49.984 kb on + strand, within Pf1N1B4_42at 49.984 kb on + strand, within Pf1N1B4_42at 49.985 kb on - strand, within Pf1N1B4_42at 49.999 kb on + strand, within Pf1N1B4_42at 49.999 kb on + strand, within Pf1N1B4_42at 50.000 kb on - strand, within Pf1N1B4_42at 50.000 kb on - strandat 50.212 kb on + strandat 50.212 kb on + strand, within Pf1N1B4_42at 50.213 kb on - strand, within Pf1N1B4_42at 50.412 kb on - strand, within Pf1N1B4_42at 50.595 kb on - strandat 50.710 kb on + strandat 50.841 kb on + strandat 50.904 kb on - strandat 50.959 kb on + strandat 50.987 kb on + strandat 50.987 kb on + strandat 50.987 kb on + strandat 51.128 kb on - strandat 51.142 kb on - strand, within Pf1N1B4_43at 51.318 kb on + strand, within Pf1N1B4_43at 51.318 kb on + strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.319 kb on - strand, within Pf1N1B4_43at 51.417 kb on + strand, within Pf1N1B4_43at 51.418 kb on - strandat 51.418 kb on - strand, within Pf1N1B4_43at 51.543 kb on + strand, within Pf1N1B4_43at 51.543 kb on + strand, within Pf1N1B4_43at 51.544 kb on - strand, within Pf1N1B4_43at 51.579 kb on + strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.580 kb on - strandat 51.581 kb on + strandat 51.582 kb on - strandat 51.582 kb on - strandat 51.582 kb on - strandat 51.582 kb on - strandat 51.664 kb on + strandat 51.665 kb on - strandat 51.716 kb on + strandat 51.716 kb on + strandat 51.716 kb on + strandat 51.717 kb on - strandat 51.717 kb on - strandat 51.717 kb on - strandat 51.717 kb on - strandat 51.717 kb on - strandat 52.145 kb on + strand, within Pf1N1B4_44at 52.146 kb on - strand, within Pf1N1B4_44at 52.146 kb on - strand, within Pf1N1B4_44at 52.146 kb on - strand, within Pf1N1B4_44at 52.146 kb on - strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.162 kb on + strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strandat 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.163 kb on - strand, within Pf1N1B4_44at 52.168 kb on + strand, within Pf1N1B4_44at 52.169 kb on - strand, within Pf1N1B4_44at 52.169 kb on - strand, within Pf1N1B4_44at 52.169 kb on - strand, within Pf1N1B4_44at 52.198 kb on + strand, within Pf1N1B4_44at 52.198 kb on + strand, within Pf1N1B4_44at 52.198 kb on + strand, within Pf1N1B4_44at 52.198 kb on + strand, within Pf1N1B4_44at 52.199 kb on - strand, within Pf1N1B4_44at 52.199 kb on - strand, within Pf1N1B4_44at 52.199 kb on - strand, within Pf1N1B4_44at 52.199 kb on - strand, within Pf1N1B4_44at 52.300 kb on + strand, within Pf1N1B4_44at 52.300 kb on + strand, within Pf1N1B4_44at 52.379 kb on - strand, within Pf1N1B4_44at 52.379 kb on - strand, within Pf1N1B4_44at 52.385 kb on - strand, within Pf1N1B4_44at 52.385 kb on - strand, within Pf1N1B4_44at 52.385 kb on - strand, within Pf1N1B4_44at 52.519 kb on + strand, within Pf1N1B4_44at 52.519 kb on + strand, within Pf1N1B4_44at 52.519 kb on + strand, within Pf1N1B4_44at 52.520 kb on - strand, within Pf1N1B4_44at 52.520 kb on - strand, within Pf1N1B4_44at 52.520 kb on - strand, within Pf1N1B4_44at 52.525 kb on + strandat 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strandat 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strand, within Pf1N1B4_44at 52.525 kb on + strand, within Pf1N1B4_44at 52.526 kb on - strand, within Pf1N1B4_44at 52.526 kb on - strand, within Pf1N1B4_44at 52.526 kb on - strand, within Pf1N1B4_44at 52.526 kb on - strand, within Pf1N1B4_44at 52.529 kb on + strand, within Pf1N1B4_44at 52.529 kb on + strand, within Pf1N1B4_44at 52.530 kb on - strand, within Pf1N1B4_44at 52.530 kb on - strand, within Pf1N1B4_44at 52.530 kb on - strand, within Pf1N1B4_44at 52.624 kb on + strand, within Pf1N1B4_44

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1
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49,984 + Pf1N1B4_42 0.59 +0.0
49,984 + Pf1N1B4_42 0.59 +0.0
49,985 - Pf1N1B4_42 0.59 +0.0
49,999 + Pf1N1B4_42 0.60 +0.0
49,999 + Pf1N1B4_42 0.60 +0.0
50,000 - Pf1N1B4_42 0.60 +0.0
50,000 - -0.1
50,212 + +0.0
50,212 + Pf1N1B4_42 0.74 -1.3
50,213 - Pf1N1B4_42 0.74 +0.0
50,412 - Pf1N1B4_42 0.87 -1.7
50,595 - +0.0
50,710 + -2.1
50,841 + +0.0
50,904 - +0.0
50,959 + +0.0
50,987 + -2.5
50,987 + +0.0
50,987 + -1.3
51,128 - +0.0
51,142 - Pf1N1B4_43 0.30 +0.0
51,318 + Pf1N1B4_43 0.54 +0.0
51,318 + Pf1N1B4_43 0.54 +0.0
51,319 - Pf1N1B4_43 0.55 -2.6
51,319 - Pf1N1B4_43 0.55 +0.0
51,319 - Pf1N1B4_43 0.55 +0.0
51,319 - Pf1N1B4_43 0.55 -2.3
51,319 - Pf1N1B4_43 0.55 +0.0
51,319 - Pf1N1B4_43 0.55 +0.0
51,319 - Pf1N1B4_43 0.55 +0.0
51,319 - Pf1N1B4_43 0.55 -2.2
51,319 - Pf1N1B4_43 0.55 -1.6
51,319 - Pf1N1B4_43 0.55 -2.8
51,417 + Pf1N1B4_43 0.69 +0.0
51,418 - +0.0
51,418 - Pf1N1B4_43 0.69 -1.9
51,543 + Pf1N1B4_43 0.86 -2.6
51,543 + Pf1N1B4_43 0.86 -3.3
51,544 - Pf1N1B4_43 0.87 +0.0
51,579 + -2.6
51,580 - -1.0
51,580 - +0.0
51,580 - +0.0
51,580 - -1.3
51,580 - +0.0
51,580 - +0.0
51,581 + -0.8
51,582 - -2.6
51,582 - -2.7
51,582 - +0.0
51,582 - +0.0
51,664 + -1.5
51,665 - +0.0
51,716 + -1.2
51,716 + +0.0
51,716 + -1.4
51,717 - -2.0
51,717 - +0.0
51,717 - -3.2
51,717 - +0.0
51,717 - +0.0
52,145 + Pf1N1B4_44 0.20 -2.2
52,146 - Pf1N1B4_44 0.20 +0.0
52,146 - Pf1N1B4_44 0.20 -1.3
52,146 - Pf1N1B4_44 0.20 +0.0
52,146 - Pf1N1B4_44 0.20 +0.0
52,162 + Pf1N1B4_44 0.21 +0.0
52,162 + Pf1N1B4_44 0.21 +0.0
52,162 + Pf1N1B4_44 0.21 -1.1
52,162 + Pf1N1B4_44 0.21 -1.6
52,162 + Pf1N1B4_44 0.21 -0.7
52,163 - Pf1N1B4_44 0.21 +0.0
52,163 - -1.5
52,163 - Pf1N1B4_44 0.21 -2.1
52,163 - Pf1N1B4_44 0.21 +0.0
52,163 - Pf1N1B4_44 0.21 +0.0
52,163 - Pf1N1B4_44 0.21 +0.0
52,163 - Pf1N1B4_44 0.21 +0.0
52,163 - Pf1N1B4_44 0.21 +0.0
52,163 - Pf1N1B4_44 0.21 -1.6
52,163 - Pf1N1B4_44 0.21 -3.0
52,163 - Pf1N1B4_44 0.21 -1.6
52,163 - Pf1N1B4_44 0.21 -1.5
52,163 - Pf1N1B4_44 0.21 +0.0
52,163 - Pf1N1B4_44 0.21 -2.1
52,163 - Pf1N1B4_44 0.21 +0.0
52,163 - Pf1N1B4_44 0.21 +0.0
52,168 + Pf1N1B4_44 0.21 -1.9
52,169 - Pf1N1B4_44 0.21 +0.0
52,169 - Pf1N1B4_44 0.21 +0.0
52,169 - Pf1N1B4_44 0.21 +0.0
52,198 + Pf1N1B4_44 0.23 +0.0
52,198 + Pf1N1B4_44 0.23 +0.0
52,198 + Pf1N1B4_44 0.23 +0.0
52,198 + Pf1N1B4_44 0.23 +0.0
52,199 - Pf1N1B4_44 0.23 +0.0
52,199 - Pf1N1B4_44 0.23 +0.0
52,199 - Pf1N1B4_44 0.23 +0.0
52,199 - Pf1N1B4_44 0.23 +0.0
52,300 + Pf1N1B4_44 0.29 -4.8
52,300 + Pf1N1B4_44 0.29 +0.0
52,379 - Pf1N1B4_44 0.33 -2.4
52,379 - Pf1N1B4_44 0.33 -2.0
52,385 - Pf1N1B4_44 0.34 +0.0
52,385 - Pf1N1B4_44 0.34 -2.6
52,385 - Pf1N1B4_44 0.34 +0.0
52,519 + Pf1N1B4_44 0.41 +0.0
52,519 + Pf1N1B4_44 0.41 -2.1
52,519 + Pf1N1B4_44 0.41 +0.0
52,520 - Pf1N1B4_44 0.41 +0.0
52,520 - Pf1N1B4_44 0.41 +0.0
52,520 - Pf1N1B4_44 0.41 +0.0
52,525 + -2.2
52,525 + Pf1N1B4_44 0.41 +0.0
52,525 + Pf1N1B4_44 0.41 -1.9
52,525 + +0.0
52,525 + Pf1N1B4_44 0.41 +0.0
52,525 + Pf1N1B4_44 0.41 +0.0
52,525 + Pf1N1B4_44 0.41 -2.4
52,525 + Pf1N1B4_44 0.41 -0.8
52,526 - Pf1N1B4_44 0.41 +0.0
52,526 - Pf1N1B4_44 0.41 +0.0
52,526 - Pf1N1B4_44 0.41 +0.0
52,526 - Pf1N1B4_44 0.41 +0.0
52,529 + Pf1N1B4_44 0.42 +0.5
52,529 + Pf1N1B4_44 0.42 -0.5
52,530 - Pf1N1B4_44 0.42 +0.0
52,530 - Pf1N1B4_44 0.42 +0.0
52,530 - Pf1N1B4_44 0.42 +0.0
52,624 + Pf1N1B4_44 0.47 -1.8

Or see this region's nucleotide sequence