Experiment: Nitrate reduction, replicate B t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2966 and Pf1N1B4_2967 are separated by 145 nucleotides Pf1N1B4_2967 and Pf1N1B4_2968 are separated by 24 nucleotides Pf1N1B4_2968 and Pf1N1B4_2969 are separated by 90 nucleotides
Pf1N1B4_2966 - FIG00958649: hypothetical protein, at 3,327,726 to 3,328,160
_2966
Pf1N1B4_2967 - Alpha/beta hydrolase, at 3,328,306 to 3,328,938
_2967
Pf1N1B4_2968 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 3,328,963 to 3,330,318
_2968
Pf1N1B4_2969 - ATPase, AFG1 family, at 3,330,409 to 3,331,503
_2969
Position (kb)
3328
3329
3330
3331 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3328.009 kb on + strand, within Pf1N1B4_2966 at 3328.174 kb on - strand at 3328.174 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.191 kb on - strand at 3328.216 kb on + strand at 3328.217 kb on - strand at 3328.217 kb on - strand at 3328.219 kb on - strand at 3328.238 kb on - strand at 3328.238 kb on - strand at 3328.260 kb on + strand at 3328.323 kb on - strand at 3328.323 kb on - strand at 3328.360 kb on + strand at 3328.360 kb on + strand at 3328.361 kb on - strand at 3328.488 kb on + strand, within Pf1N1B4_2967 at 3328.489 kb on - strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.761 kb on + strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.762 kb on - strand, within Pf1N1B4_2967 at 3328.816 kb on + strand, within Pf1N1B4_2967 at 3328.816 kb on + strand at 3330.371 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.419 kb on + strand at 3330.419 kb on + strand at 3330.419 kb on + strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.506 kb on - strand at 3330.506 kb on - strand at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1 remove 3,328,009 + Pf1N1B4_2966 0.65 +0.0 3,328,174 - -2.4 3,328,174 - +0.0 3,328,191 - -2.2 3,328,191 - +0.0 3,328,191 - -1.2 3,328,191 - +0.0 3,328,191 - -2.4 3,328,191 - +0.0 3,328,216 + +0.0 3,328,217 - -1.0 3,328,217 - -0.9 3,328,219 - -2.3 3,328,238 - +0.0 3,328,238 - +0.0 3,328,260 + -2.6 3,328,323 - +0.0 3,328,323 - +0.0 3,328,360 + +0.0 3,328,360 + +0.0 3,328,361 - +0.0 3,328,488 + Pf1N1B4_2967 0.29 +0.0 3,328,489 - Pf1N1B4_2967 0.29 -3.4 3,328,761 + Pf1N1B4_2967 0.72 -0.9 3,328,761 + Pf1N1B4_2967 0.72 +0.0 3,328,761 + Pf1N1B4_2967 0.72 +0.0 3,328,761 + Pf1N1B4_2967 0.72 +0.0 3,328,761 + Pf1N1B4_2967 0.72 +0.0 3,328,761 + Pf1N1B4_2967 0.72 -2.6 3,328,761 + Pf1N1B4_2967 0.72 -1.1 3,328,761 + Pf1N1B4_2967 0.72 +0.0 3,328,762 - Pf1N1B4_2967 0.72 +0.0 3,328,762 - Pf1N1B4_2967 0.72 +0.0 3,328,762 - Pf1N1B4_2967 0.72 +0.0 3,328,762 - Pf1N1B4_2967 0.72 +0.0 3,328,762 - Pf1N1B4_2967 0.72 -3.7 3,328,762 - Pf1N1B4_2967 0.72 +0.0 3,328,762 - Pf1N1B4_2967 0.72 +0.0 3,328,762 - Pf1N1B4_2967 0.72 -3.2 3,328,762 - Pf1N1B4_2967 0.72 +0.0 3,328,762 - Pf1N1B4_2967 0.72 -2.7 3,328,762 - Pf1N1B4_2967 0.72 -2.5 3,328,816 + Pf1N1B4_2967 0.81 +0.0 3,328,816 + +0.0 3,330,371 - -2.0 3,330,409 - -1.6 3,330,409 - +0.0 3,330,409 - -3.0 3,330,409 - -2.0 3,330,409 - -2.2 3,330,409 - -2.7 3,330,409 - +0.0 3,330,419 + +0.0 3,330,419 + -1.6 3,330,419 + +0.0 3,330,420 - -1.6 3,330,420 - +0.0 3,330,420 - -3.7 3,330,420 - +0.0 3,330,420 - -2.1 3,330,420 - -1.6 3,330,505 + -0.6 3,330,505 + -2.6 3,330,505 + +0.0 3,330,505 + +0.0 3,330,505 + +0.0 3,330,506 - +0.0 3,330,506 - +0.0 3,330,595 + Pf1N1B4_2969 0.17 -2.5 3,330,595 + Pf1N1B4_2969 0.17 +0.0 3,330,595 + Pf1N1B4_2969 0.17 -1.5 3,330,595 + Pf1N1B4_2969 0.17 +0.0 3,330,595 + Pf1N1B4_2969 0.17 +0.0 3,330,595 + Pf1N1B4_2969 0.17 +0.0 3,330,595 + Pf1N1B4_2969 0.17 -2.0 3,330,595 + Pf1N1B4_2969 0.17 -1.6 3,330,596 - Pf1N1B4_2969 0.17 +0.0 3,330,596 - Pf1N1B4_2969 0.17 -2.0 3,330,596 - Pf1N1B4_2969 0.17 +0.0 3,330,596 - Pf1N1B4_2969 0.17 +0.0 3,330,596 - Pf1N1B4_2969 0.17 +0.0 3,330,596 - Pf1N1B4_2969 0.17 -1.6 3,330,596 - Pf1N1B4_2969 0.17 +2.0 3,330,596 - Pf1N1B4_2969 0.17 +0.0 3,330,596 - Pf1N1B4_2969 0.17 -1.6 3,330,596 - Pf1N1B4_2969 0.17 -1.0 3,330,596 - Pf1N1B4_2969 0.17 -0.6 3,330,596 - Pf1N1B4_2969 0.17 +0.0 3,330,628 + Pf1N1B4_2969 0.20 -2.2 3,330,628 + Pf1N1B4_2969 0.20 -1.6 3,330,628 + Pf1N1B4_2969 0.20 -1.0 3,330,628 + Pf1N1B4_2969 0.20 +0.0 3,330,628 + Pf1N1B4_2969 0.20 +0.0 3,330,628 + Pf1N1B4_2969 0.20 +0.0 3,330,628 + Pf1N1B4_2969 0.20 -1.6 3,330,628 + Pf1N1B4_2969 0.20 -1.6 3,330,628 + Pf1N1B4_2969 0.20 -1.2 3,330,628 + Pf1N1B4_2969 0.20 +0.0 3,330,628 + Pf1N1B4_2969 0.20 -2.8 3,330,628 + Pf1N1B4_2969 0.20 -2.3 3,330,628 + Pf1N1B4_2969 0.20 -3.2 3,330,628 + Pf1N1B4_2969 0.20 +0.0 3,330,629 - Pf1N1B4_2969 0.20 -1.9 3,330,629 - Pf1N1B4_2969 0.20 +0.0 3,330,629 - Pf1N1B4_2969 0.20 +0.0 3,330,629 - Pf1N1B4_2969 0.20 +0.0 3,330,629 - Pf1N1B4_2969 0.20 +0.0 3,330,629 - Pf1N1B4_2969 0.20 -2.0 3,330,629 - Pf1N1B4_2969 0.20 -1.6 3,330,629 - Pf1N1B4_2969 0.20 -1.7 3,330,629 - Pf1N1B4_2969 0.20 +0.0 3,330,629 - Pf1N1B4_2969 0.20 +0.0 3,330,629 - Pf1N1B4_2969 0.20 -2.3 3,330,629 - Pf1N1B4_2969 0.20 +0.0 3,330,629 - Pf1N1B4_2969 0.20 -1.6 3,330,629 - Pf1N1B4_2969 0.20 -2.8 3,330,629 - Pf1N1B4_2969 0.20 -1.6 3,330,629 - Pf1N1B4_2969 0.20 -2.0 3,330,629 - Pf1N1B4_2969 0.20 -1.7 3,330,916 + Pf1N1B4_2969 0.46 -3.3 3,330,916 + Pf1N1B4_2969 0.46 -3.0 3,330,916 + Pf1N1B4_2969 0.46 -1.6 3,330,916 + Pf1N1B4_2969 0.46 +0.0 3,330,916 + Pf1N1B4_2969 0.46 -0.9 3,330,916 + Pf1N1B4_2969 0.46 +0.0 3,330,916 + -1.6 3,330,916 + Pf1N1B4_2969 0.46 -1.0 3,330,916 + Pf1N1B4_2969 0.46 -0.7 3,330,916 + Pf1N1B4_2969 0.46 -2.8 3,330,916 + Pf1N1B4_2969 0.46 +0.0 3,330,917 - Pf1N1B4_2969 0.46 +0.0 3,330,917 - Pf1N1B4_2969 0.46 -2.8 3,330,917 - Pf1N1B4_2969 0.46 -0.9 3,330,917 - Pf1N1B4_2969 0.46 +0.0 3,330,917 - Pf1N1B4_2969 0.46 -2.4 3,330,917 - Pf1N1B4_2969 0.46 +0.0 3,330,917 - Pf1N1B4_2969 0.46 +0.0 3,331,136 + Pf1N1B4_2969 0.66 -1.6 3,331,136 + Pf1N1B4_2969 0.66 -2.3 3,331,136 + Pf1N1B4_2969 0.66 -1.1 3,331,136 + Pf1N1B4_2969 0.66 -2.1 3,331,136 + Pf1N1B4_2969 0.66 +0.0 3,331,136 + Pf1N1B4_2969 0.66 +0.0 3,331,136 + Pf1N1B4_2969 0.66 +0.0 3,331,136 + Pf1N1B4_2969 0.66 -1.7 3,331,136 + Pf1N1B4_2969 0.66 -1.6 3,331,136 + Pf1N1B4_2969 0.66 -1.4 3,331,136 + Pf1N1B4_2969 0.66 +0.0 3,331,136 + Pf1N1B4_2969 0.66 -1.9 3,331,136 + Pf1N1B4_2969 0.66 +0.0 3,331,136 + Pf1N1B4_2969 0.66 +0.0 3,331,136 + Pf1N1B4_2969 0.66 -1.8 3,331,136 + Pf1N1B4_2969 0.66 +0.0 3,331,136 + Pf1N1B4_2969 0.66 -0.6 3,331,136 + Pf1N1B4_2969 0.66 -1.3 3,331,136 + Pf1N1B4_2969 0.66 -1.0 3,331,136 + Pf1N1B4_2969 0.66 +0.0 3,331,136 + -3.8 3,331,136 + Pf1N1B4_2969 0.66 -2.2 3,331,136 + Pf1N1B4_2969 0.66 -2.4 3,331,137 - Pf1N1B4_2969 0.66 -0.7 3,331,137 - Pf1N1B4_2969 0.66 -2.3 3,331,137 - Pf1N1B4_2969 0.66 +0.0 3,331,137 - Pf1N1B4_2969 0.66 -3.8 3,331,137 - Pf1N1B4_2969 0.66 -2.3 3,331,137 - Pf1N1B4_2969 0.66 +0.0 3,331,137 - Pf1N1B4_2969 0.66 +0.0 3,331,137 - Pf1N1B4_2969 0.66 -1.3 3,331,137 - Pf1N1B4_2969 0.66 +0.0 3,331,137 - Pf1N1B4_2969 0.66 -3.0 3,331,137 - Pf1N1B4_2969 0.66 -0.8 3,331,137 - Pf1N1B4_2969 0.66 -1.6 3,331,240 + Pf1N1B4_2969 0.76 +0.0 3,331,240 + Pf1N1B4_2969 0.76 +0.0 3,331,240 + +0.6 3,331,240 + Pf1N1B4_2969 0.76 -1.3 3,331,240 + Pf1N1B4_2969 0.76 -1.5 3,331,240 + Pf1N1B4_2969 0.76 -3.1 3,331,240 + Pf1N1B4_2969 0.76 +0.0 3,331,240 + Pf1N1B4_2969 0.76 +0.0 3,331,240 + Pf1N1B4_2969 0.76 -1.9 3,331,240 + Pf1N1B4_2969 0.76 -2.8 3,331,241 - Pf1N1B4_2969 0.76 +0.0 3,331,241 - Pf1N1B4_2969 0.76 +0.0 3,331,241 - Pf1N1B4_2969 0.76 +0.0 3,331,241 - Pf1N1B4_2969 0.76 -2.1 3,331,241 - Pf1N1B4_2969 0.76 -2.6 3,331,241 - Pf1N1B4_2969 0.76 -0.9 3,331,241 - Pf1N1B4_2969 0.76 +0.0
Or see this region's nucleotide sequence