Experiment: Nitrate reduction, replicate B t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2289 and Pf1N1B4_2290 are separated by 127 nucleotides Pf1N1B4_2290 and Pf1N1B4_2291 are separated by 84 nucleotides
Pf1N1B4_2289 - Thymidylate synthase (EC 2.1.1.45), at 2,604,895 to 2,605,866
_2289
Pf1N1B4_2290 - Cd(II)/Pb(II)-responsive transcriptional regulator, at 2,605,994 to 2,606,443
_2290
Pf1N1B4_2291 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 2,606,528 to 2,608,786
_2291
Position (kb)
2605
2606
2607 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2605.882 kb on - strand at 2605.897 kb on - strand at 2605.966 kb on - strand at 2605.995 kb on + strand at 2605.995 kb on + strand at 2605.996 kb on - strand at 2605.996 kb on - strand at 2606.328 kb on + strand, within Pf1N1B4_2290 at 2606.328 kb on + strand, within Pf1N1B4_2290 at 2606.328 kb on + strand, within Pf1N1B4_2290 at 2606.329 kb on - strand, within Pf1N1B4_2290 at 2606.329 kb on - strand, within Pf1N1B4_2290 at 2606.329 kb on - strand, within Pf1N1B4_2290 at 2606.385 kb on + strand, within Pf1N1B4_2290 at 2606.386 kb on - strand, within Pf1N1B4_2290 at 2606.386 kb on - strand, within Pf1N1B4_2290 at 2606.475 kb on - strand at 2606.494 kb on + strand at 2606.494 kb on + strand at 2606.495 kb on - strand at 2606.495 kb on - strand at 2606.495 kb on - strand at 2606.983 kb on + strand, within Pf1N1B4_2291 at 2606.984 kb on - strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.065 kb on + strand, within Pf1N1B4_2291 at 2607.066 kb on - strand, within Pf1N1B4_2291 at 2607.387 kb on + strand, within Pf1N1B4_2291 at 2607.387 kb on + strand, within Pf1N1B4_2291 at 2607.387 kb on + strand, within Pf1N1B4_2291 at 2607.388 kb on - strand, within Pf1N1B4_2291 at 2607.388 kb on - strand, within Pf1N1B4_2291 at 2607.388 kb on - strand, within Pf1N1B4_2291 at 2607.388 kb on - strand, within Pf1N1B4_2291
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1 remove 2,605,882 - -0.1 2,605,897 - -0.3 2,605,966 - -0.1 2,605,995 + -1.9 2,605,995 + -0.1 2,605,996 - -0.1 2,605,996 - -0.1 2,606,328 + Pf1N1B4_2290 0.74 -0.1 2,606,328 + Pf1N1B4_2290 0.74 -2.6 2,606,328 + Pf1N1B4_2290 0.74 -0.1 2,606,329 - Pf1N1B4_2290 0.74 -3.5 2,606,329 - Pf1N1B4_2290 0.74 -0.1 2,606,329 - Pf1N1B4_2290 0.74 -1.1 2,606,385 + Pf1N1B4_2290 0.87 -0.1 2,606,386 - Pf1N1B4_2290 0.87 -0.1 2,606,386 - Pf1N1B4_2290 0.87 -2.4 2,606,475 - -0.1 2,606,494 + -0.1 2,606,494 + -1.7 2,606,495 - -0.1 2,606,495 - -0.1 2,606,495 - -0.1 2,606,983 + Pf1N1B4_2291 0.20 -0.1 2,606,984 - Pf1N1B4_2291 0.20 -0.1 2,607,065 + Pf1N1B4_2291 0.24 -0.1 2,607,065 + Pf1N1B4_2291 0.24 -0.1 2,607,065 + Pf1N1B4_2291 0.24 -2.1 2,607,065 + Pf1N1B4_2291 0.24 -0.1 2,607,065 + Pf1N1B4_2291 0.24 -0.1 2,607,065 + Pf1N1B4_2291 0.24 -0.1 2,607,066 - Pf1N1B4_2291 0.24 -0.1 2,607,387 + Pf1N1B4_2291 0.38 -5.0 2,607,387 + Pf1N1B4_2291 0.38 -0.3 2,607,387 + Pf1N1B4_2291 0.38 -0.1 2,607,388 - Pf1N1B4_2291 0.38 -1.5 2,607,388 - Pf1N1B4_2291 0.38 -0.1 2,607,388 - Pf1N1B4_2291 0.38 -0.1 2,607,388 - Pf1N1B4_2291 0.38 -0.1
Or see this region's nucleotide sequence