Experiment: Nitrate reduction, replicate B t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2121 and Pf1N1B4_2122 are separated by 25 nucleotides Pf1N1B4_2122 and Pf1N1B4_2123 overlap by 4 nucleotides Pf1N1B4_2123 and Pf1N1B4_2124 are separated by 65 nucleotides Pf1N1B4_2124 and Pf1N1B4_2125 are separated by 5 nucleotides
Pf1N1B4_2121 - Acetylglutamate kinase (EC 2.7.2.8), at 2,432,291 to 2,433,196
_2121
Pf1N1B4_2122 - Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2), at 2,433,222 to 2,434,619
_2122
Pf1N1B4_2123 - hypothetical protein, at 2,434,616 to 2,434,816
_2123
Pf1N1B4_2124 - Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23), at 2,434,882 to 2,435,337
_2124
Pf1N1B4_2125 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 2,435,343 to 2,436,551
_2125
Position (kb)
2433
2434
2435 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2432.321 kb on - strand at 2433.219 kb on - strand at 2433.219 kb on - strand at 2433.219 kb on - strand at 2433.377 kb on - strand, within Pf1N1B4_2122 at 2433.377 kb on - strand, within Pf1N1B4_2122 at 2433.377 kb on - strand, within Pf1N1B4_2122 at 2433.377 kb on - strand, within Pf1N1B4_2122 at 2433.483 kb on - strand, within Pf1N1B4_2122 at 2433.497 kb on + strand, within Pf1N1B4_2122 at 2433.497 kb on + strand, within Pf1N1B4_2122 at 2433.503 kb on + strand, within Pf1N1B4_2122 at 2433.503 kb on + strand, within Pf1N1B4_2122 at 2433.503 kb on + strand, within Pf1N1B4_2122 at 2433.504 kb on - strand, within Pf1N1B4_2122 at 2433.504 kb on - strand, within Pf1N1B4_2122 at 2433.504 kb on - strand, within Pf1N1B4_2122 at 2433.504 kb on - strand, within Pf1N1B4_2122 at 2433.583 kb on + strand, within Pf1N1B4_2122 at 2433.583 kb on + strand, within Pf1N1B4_2122 at 2433.584 kb on - strand, within Pf1N1B4_2122 at 2433.584 kb on - strand, within Pf1N1B4_2122 at 2433.584 kb on - strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.626 kb on + strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2433.627 kb on - strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.138 kb on + strand, within Pf1N1B4_2122 at 2434.139 kb on - strand, within Pf1N1B4_2122 at 2434.139 kb on - strand, within Pf1N1B4_2122 at 2434.273 kb on + strand, within Pf1N1B4_2122 at 2434.273 kb on + strand, within Pf1N1B4_2122 at 2434.274 kb on - strand, within Pf1N1B4_2122 at 2434.274 kb on - strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.326 kb on + strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.327 kb on - strand, within Pf1N1B4_2122 at 2434.339 kb on + strand, within Pf1N1B4_2122 at 2434.339 kb on + strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.340 kb on - strand, within Pf1N1B4_2122 at 2434.342 kb on + strand, within Pf1N1B4_2122 at 2434.342 kb on + strand, within Pf1N1B4_2122 at 2434.342 kb on + strand, within Pf1N1B4_2122 at 2434.343 kb on - strand, within Pf1N1B4_2122 at 2434.343 kb on - strand, within Pf1N1B4_2122 at 2434.343 kb on - strand, within Pf1N1B4_2122 at 2434.343 kb on - strand, within Pf1N1B4_2122 at 2434.461 kb on + strand, within Pf1N1B4_2122 at 2434.461 kb on + strand, within Pf1N1B4_2122 at 2434.462 kb on - strand, within Pf1N1B4_2122 at 2434.462 kb on - strand, within Pf1N1B4_2122 at 2434.516 kb on + strand at 2434.517 kb on - strand at 2434.517 kb on - strand at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.640 kb on + strand, within Pf1N1B4_2123 at 2434.641 kb on - strand, within Pf1N1B4_2123 at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.694 kb on + strand at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.694 kb on + strand, within Pf1N1B4_2123 at 2434.695 kb on - strand, within Pf1N1B4_2123 at 2434.729 kb on + strand, within Pf1N1B4_2123 at 2434.729 kb on + strand, within Pf1N1B4_2123 at 2434.729 kb on + strand, within Pf1N1B4_2123 at 2434.729 kb on + strand, within Pf1N1B4_2123 at 2434.730 kb on - strand, within Pf1N1B4_2123 at 2434.730 kb on - strand, within Pf1N1B4_2123 at 2434.790 kb on + strand, within Pf1N1B4_2123 at 2434.791 kb on - strand, within Pf1N1B4_2123 at 2434.828 kb on - strand at 2435.345 kb on - strand at 2435.345 kb on - strand at 2435.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1 remove 2,432,321 - -0.1 2,433,219 - -1.3 2,433,219 - -0.1 2,433,219 - -0.1 2,433,377 - Pf1N1B4_2122 0.11 -0.1 2,433,377 - Pf1N1B4_2122 0.11 -0.1 2,433,377 - Pf1N1B4_2122 0.11 -0.1 2,433,377 - Pf1N1B4_2122 0.11 -0.1 2,433,483 - Pf1N1B4_2122 0.19 -0.1 2,433,497 + Pf1N1B4_2122 0.20 -0.1 2,433,497 + Pf1N1B4_2122 0.20 +0.9 2,433,503 + Pf1N1B4_2122 0.20 -1.4 2,433,503 + Pf1N1B4_2122 0.20 -0.1 2,433,503 + Pf1N1B4_2122 0.20 -0.1 2,433,504 - Pf1N1B4_2122 0.20 -2.0 2,433,504 - Pf1N1B4_2122 0.20 -0.1 2,433,504 - Pf1N1B4_2122 0.20 -0.1 2,433,504 - Pf1N1B4_2122 0.20 -1.3 2,433,583 + Pf1N1B4_2122 0.26 -0.1 2,433,583 + Pf1N1B4_2122 0.26 -0.1 2,433,584 - Pf1N1B4_2122 0.26 -0.1 2,433,584 - Pf1N1B4_2122 0.26 -0.1 2,433,584 - Pf1N1B4_2122 0.26 -0.1 2,433,626 + Pf1N1B4_2122 0.29 -0.1 2,433,626 + Pf1N1B4_2122 0.29 +0.8 2,433,626 + Pf1N1B4_2122 0.29 -0.1 2,433,626 + Pf1N1B4_2122 0.29 -0.1 2,433,626 + Pf1N1B4_2122 0.29 -0.1 2,433,627 - Pf1N1B4_2122 0.29 -0.1 2,433,627 - Pf1N1B4_2122 0.29 -0.1 2,433,627 - Pf1N1B4_2122 0.29 -0.1 2,434,138 + Pf1N1B4_2122 0.66 -5.1 2,434,138 + Pf1N1B4_2122 0.66 -1.6 2,434,138 + Pf1N1B4_2122 0.66 -0.1 2,434,139 - Pf1N1B4_2122 0.66 -1.4 2,434,139 - Pf1N1B4_2122 0.66 -0.1 2,434,273 + Pf1N1B4_2122 0.75 -0.7 2,434,273 + Pf1N1B4_2122 0.75 -0.1 2,434,274 - Pf1N1B4_2122 0.75 -0.1 2,434,274 - Pf1N1B4_2122 0.75 -0.1 2,434,326 + Pf1N1B4_2122 0.79 -0.1 2,434,326 + Pf1N1B4_2122 0.79 -0.1 2,434,326 + Pf1N1B4_2122 0.79 -0.1 2,434,326 + Pf1N1B4_2122 0.79 -2.0 2,434,326 + Pf1N1B4_2122 0.79 -0.1 2,434,326 + Pf1N1B4_2122 0.79 -0.1 2,434,326 + Pf1N1B4_2122 0.79 -0.1 2,434,326 + Pf1N1B4_2122 0.79 -1.5 2,434,326 + Pf1N1B4_2122 0.79 -2.3 2,434,326 + Pf1N1B4_2122 0.79 -0.1 2,434,326 + Pf1N1B4_2122 0.79 -0.1 2,434,327 - Pf1N1B4_2122 0.79 -1.0 2,434,327 - Pf1N1B4_2122 0.79 -0.1 2,434,327 - Pf1N1B4_2122 0.79 -0.1 2,434,327 - Pf1N1B4_2122 0.79 -0.6 2,434,327 - Pf1N1B4_2122 0.79 -0.1 2,434,339 + Pf1N1B4_2122 0.80 -0.1 2,434,339 + Pf1N1B4_2122 0.80 -0.1 2,434,340 - Pf1N1B4_2122 0.80 -0.4 2,434,340 - Pf1N1B4_2122 0.80 -0.1 2,434,340 - Pf1N1B4_2122 0.80 -0.1 2,434,340 - Pf1N1B4_2122 0.80 -2.2 2,434,340 - Pf1N1B4_2122 0.80 -0.1 2,434,342 + Pf1N1B4_2122 0.80 -2.0 2,434,342 + Pf1N1B4_2122 0.80 -0.1 2,434,342 + Pf1N1B4_2122 0.80 -0.1 2,434,343 - Pf1N1B4_2122 0.80 -1.1 2,434,343 - Pf1N1B4_2122 0.80 -0.6 2,434,343 - Pf1N1B4_2122 0.80 -0.1 2,434,343 - Pf1N1B4_2122 0.80 -0.1 2,434,461 + Pf1N1B4_2122 0.89 -0.1 2,434,461 + Pf1N1B4_2122 0.89 -0.1 2,434,462 - Pf1N1B4_2122 0.89 -0.1 2,434,462 - Pf1N1B4_2122 0.89 -0.1 2,434,516 + -0.1 2,434,517 - -0.1 2,434,517 - -0.1 2,434,640 + Pf1N1B4_2123 0.12 -0.1 2,434,640 + Pf1N1B4_2123 0.12 -0.1 2,434,640 + Pf1N1B4_2123 0.12 -0.1 2,434,640 + Pf1N1B4_2123 0.12 -2.2 2,434,640 + Pf1N1B4_2123 0.12 -0.9 2,434,640 + Pf1N1B4_2123 0.12 -1.6 2,434,641 - Pf1N1B4_2123 0.12 -1.1 2,434,694 + Pf1N1B4_2123 0.39 -1.6 2,434,694 + Pf1N1B4_2123 0.39 -0.9 2,434,694 + -0.1 2,434,694 + Pf1N1B4_2123 0.39 -0.1 2,434,694 + Pf1N1B4_2123 0.39 -1.8 2,434,694 + Pf1N1B4_2123 0.39 -2.6 2,434,695 - Pf1N1B4_2123 0.39 -1.4 2,434,729 + Pf1N1B4_2123 0.56 -0.1 2,434,729 + Pf1N1B4_2123 0.56 -0.1 2,434,729 + Pf1N1B4_2123 0.56 -0.1 2,434,729 + Pf1N1B4_2123 0.56 -0.1 2,434,730 - Pf1N1B4_2123 0.57 -1.0 2,434,730 - Pf1N1B4_2123 0.57 -0.1 2,434,790 + Pf1N1B4_2123 0.87 -0.1 2,434,791 - Pf1N1B4_2123 0.87 -0.1 2,434,828 - -0.1 2,435,345 - -0.1 2,435,345 - -0.4 2,435,345 - -0.1
Or see this region's nucleotide sequence