Experiment: Nitrate reduction, replicate B t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1924 and Pf1N1B4_1925 are separated by 445 nucleotides Pf1N1B4_1925 and Pf1N1B4_1926 are separated by 72 nucleotides
Pf1N1B4_1924 - LysM domain protein, at 2,200,004 to 2,200,942
_1924
Pf1N1B4_1925 - Two-component response regulator, at 2,201,388 to 2,202,107
_1925
Pf1N1B4_1926 - DNA gyrase subunit B (EC 5.99.1.3), at 2,202,180 to 2,204,558
_1926
Position (kb)
2201
2202
2203 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2200.526 kb on + strand, within Pf1N1B4_1924 at 2200.526 kb on + strand, within Pf1N1B4_1924 at 2200.584 kb on + strand, within Pf1N1B4_1924 at 2200.584 kb on + strand, within Pf1N1B4_1924 at 2200.584 kb on + strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.585 kb on - strand, within Pf1N1B4_1924 at 2200.756 kb on + strand at 2200.756 kb on + strand, within Pf1N1B4_1924 at 2200.756 kb on + strand, within Pf1N1B4_1924 at 2200.756 kb on + strand, within Pf1N1B4_1924 at 2200.757 kb on - strand, within Pf1N1B4_1924 at 2200.757 kb on - strand, within Pf1N1B4_1924 at 2200.757 kb on - strand, within Pf1N1B4_1924 at 2200.757 kb on - strand, within Pf1N1B4_1924 at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.872 kb on + strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.873 kb on - strand at 2200.946 kb on + strand at 2200.946 kb on + strand at 2200.946 kb on + strand at 2200.946 kb on + strand at 2200.979 kb on + strand at 2200.979 kb on + strand at 2200.979 kb on + strand at 2201.010 kb on - strand at 2201.010 kb on - strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.048 kb on + strand at 2201.049 kb on - strand at 2201.049 kb on - strand at 2201.049 kb on - strand at 2201.049 kb on - strand at 2201.049 kb on - strand at 2201.049 kb on - strand at 2201.049 kb on - strand at 2201.049 kb on - strand at 2201.049 kb on - strand at 2201.062 kb on + strand at 2201.062 kb on + strand at 2201.087 kb on - strand at 2201.106 kb on - strand at 2201.280 kb on + strand at 2201.280 kb on + strand at 2201.280 kb on + strand at 2201.280 kb on + strand at 2201.280 kb on + strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.333 kb on + strand at 2201.364 kb on - strand at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.490 kb on - strand, within Pf1N1B4_1925 at 2201.490 kb on - strand, within Pf1N1B4_1925 at 2201.490 kb on - strand, within Pf1N1B4_1925 at 2201.700 kb on + strand, within Pf1N1B4_1925 at 2201.700 kb on + strand, within Pf1N1B4_1925 at 2201.700 kb on + strand, within Pf1N1B4_1925 at 2201.701 kb on - strand, within Pf1N1B4_1925 at 2201.794 kb on + strand, within Pf1N1B4_1925 at 2201.794 kb on + strand, within Pf1N1B4_1925 at 2201.794 kb on + strand at 2201.794 kb on + strand, within Pf1N1B4_1925 at 2201.795 kb on - strand, within Pf1N1B4_1925 at 2201.795 kb on - strand, within Pf1N1B4_1925 at 2201.881 kb on - strand, within Pf1N1B4_1925 at 2201.934 kb on + strand, within Pf1N1B4_1925 at 2201.934 kb on + strand, within Pf1N1B4_1925 at 2201.935 kb on - strand, within Pf1N1B4_1925 at 2201.935 kb on - strand, within Pf1N1B4_1925 at 2201.935 kb on - strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.968 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2202.001 kb on - strand, within Pf1N1B4_1925 at 2202.060 kb on + strand at 2202.060 kb on + strand at 2202.060 kb on + strand at 2202.061 kb on - strand at 2202.061 kb on - strand at 2202.127 kb on - strand at 2202.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t1 remove 2,200,526 + Pf1N1B4_1924 0.56 -0.9 2,200,526 + Pf1N1B4_1924 0.56 -0.0 2,200,584 + Pf1N1B4_1924 0.62 -2.2 2,200,584 + Pf1N1B4_1924 0.62 -0.0 2,200,584 + Pf1N1B4_1924 0.62 -0.0 2,200,585 - Pf1N1B4_1924 0.62 -0.0 2,200,585 - Pf1N1B4_1924 0.62 -1.1 2,200,585 - Pf1N1B4_1924 0.62 -0.0 2,200,585 - Pf1N1B4_1924 0.62 -1.5 2,200,585 - Pf1N1B4_1924 0.62 -0.0 2,200,585 - Pf1N1B4_1924 0.62 -2.4 2,200,585 - Pf1N1B4_1924 0.62 -0.0 2,200,585 - Pf1N1B4_1924 0.62 -1.7 2,200,585 - Pf1N1B4_1924 0.62 -0.0 2,200,585 - Pf1N1B4_1924 0.62 -2.5 2,200,585 - Pf1N1B4_1924 0.62 -0.0 2,200,756 + -2.9 2,200,756 + Pf1N1B4_1924 0.80 -1.0 2,200,756 + Pf1N1B4_1924 0.80 -0.0 2,200,756 + Pf1N1B4_1924 0.80 -0.0 2,200,757 - Pf1N1B4_1924 0.80 -0.0 2,200,757 - Pf1N1B4_1924 0.80 -4.3 2,200,757 - Pf1N1B4_1924 0.80 -0.0 2,200,757 - Pf1N1B4_1924 0.80 -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -1.1 2,200,872 + -0.0 2,200,872 + -0.0 2,200,872 + -0.1 2,200,873 - -0.0 2,200,873 - -0.0 2,200,873 - -0.0 2,200,873 - -0.0 2,200,873 - -1.9 2,200,873 - -0.9 2,200,873 - -0.0 2,200,873 - -0.0 2,200,873 - -0.0 2,200,873 - -0.0 2,200,873 - -0.0 2,200,946 + -0.0 2,200,946 + -2.0 2,200,946 + -0.0 2,200,946 + -0.0 2,200,979 + -0.0 2,200,979 + -0.0 2,200,979 + -1.5 2,201,010 - -0.0 2,201,010 - -0.0 2,201,048 + -0.0 2,201,048 + -0.0 2,201,048 + -1.5 2,201,048 + -1.4 2,201,048 + -0.0 2,201,048 + -0.0 2,201,048 + -1.1 2,201,048 + -0.0 2,201,048 + -2.7 2,201,048 + -0.0 2,201,048 + -0.0 2,201,049 - -2.0 2,201,049 - -0.8 2,201,049 - -0.0 2,201,049 - -0.7 2,201,049 - -0.0 2,201,049 - -2.3 2,201,049 - -0.0 2,201,049 - -0.0 2,201,049 - -1.0 2,201,062 + -0.0 2,201,062 + -0.0 2,201,087 - -1.9 2,201,106 - -0.0 2,201,280 + -1.3 2,201,280 + -1.0 2,201,280 + -0.0 2,201,280 + -2.5 2,201,280 + -0.0 2,201,281 - -0.0 2,201,281 - -0.0 2,201,281 - -0.0 2,201,281 - -1.6 2,201,281 - -0.0 2,201,281 - -2.0 2,201,333 + -0.0 2,201,364 - -2.1 2,201,489 + Pf1N1B4_1925 0.14 -1.2 2,201,489 + Pf1N1B4_1925 0.14 -0.0 2,201,489 + Pf1N1B4_1925 0.14 -0.0 2,201,489 + Pf1N1B4_1925 0.14 -0.0 2,201,489 + Pf1N1B4_1925 0.14 -0.0 2,201,489 + Pf1N1B4_1925 0.14 -1.3 2,201,489 + Pf1N1B4_1925 0.14 -0.0 2,201,489 + Pf1N1B4_1925 0.14 -0.8 2,201,489 + Pf1N1B4_1925 0.14 -2.4 2,201,489 + Pf1N1B4_1925 0.14 -0.0 2,201,490 - Pf1N1B4_1925 0.14 -0.0 2,201,490 - Pf1N1B4_1925 0.14 -0.0 2,201,490 - Pf1N1B4_1925 0.14 -0.0 2,201,700 + Pf1N1B4_1925 0.43 -0.0 2,201,700 + Pf1N1B4_1925 0.43 -0.0 2,201,700 + Pf1N1B4_1925 0.43 -0.0 2,201,701 - Pf1N1B4_1925 0.43 -0.0 2,201,794 + Pf1N1B4_1925 0.56 -0.0 2,201,794 + Pf1N1B4_1925 0.56 -0.0 2,201,794 + -0.0 2,201,794 + Pf1N1B4_1925 0.56 -2.5 2,201,795 - Pf1N1B4_1925 0.57 -0.0 2,201,795 - Pf1N1B4_1925 0.57 -0.0 2,201,881 - Pf1N1B4_1925 0.68 -0.0 2,201,934 + Pf1N1B4_1925 0.76 -0.0 2,201,934 + Pf1N1B4_1925 0.76 -2.4 2,201,935 - Pf1N1B4_1925 0.76 -1.6 2,201,935 - Pf1N1B4_1925 0.76 -0.0 2,201,935 - Pf1N1B4_1925 0.76 -1.4 2,201,940 + Pf1N1B4_1925 0.77 -0.0 2,201,940 + Pf1N1B4_1925 0.77 -0.0 2,201,940 + Pf1N1B4_1925 0.77 -0.0 2,201,940 + Pf1N1B4_1925 0.77 -0.0 2,201,940 + Pf1N1B4_1925 0.77 -1.0 2,201,940 + Pf1N1B4_1925 0.77 -1.9 2,201,940 + Pf1N1B4_1925 0.77 -1.7 2,201,940 + Pf1N1B4_1925 0.77 -1.1 2,201,940 + Pf1N1B4_1925 0.77 -0.0 2,201,940 + Pf1N1B4_1925 0.77 -0.6 2,201,941 - Pf1N1B4_1925 0.77 -0.0 2,201,941 - Pf1N1B4_1925 0.77 -0.0 2,201,941 - Pf1N1B4_1925 0.77 -0.0 2,201,941 - Pf1N1B4_1925 0.77 -0.0 2,201,941 - Pf1N1B4_1925 0.77 -0.0 2,201,941 - Pf1N1B4_1925 0.77 -0.0 2,201,941 - Pf1N1B4_1925 0.77 -2.7 2,201,941 - Pf1N1B4_1925 0.77 -0.0 2,201,941 - Pf1N1B4_1925 0.77 -0.0 2,201,968 + Pf1N1B4_1925 0.81 -0.0 2,201,996 + Pf1N1B4_1925 0.84 +0.5 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -2.5 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -2.1 2,201,996 + Pf1N1B4_1925 0.84 -2.2 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,996 + Pf1N1B4_1925 0.84 -0.0 2,201,997 - Pf1N1B4_1925 0.85 -0.0 2,201,997 - Pf1N1B4_1925 0.85 -0.0 2,201,997 - Pf1N1B4_1925 0.85 -0.0 2,201,997 - Pf1N1B4_1925 0.85 -3.1 2,201,997 - Pf1N1B4_1925 0.85 -0.8 2,201,997 - Pf1N1B4_1925 0.85 -0.0 2,201,997 - Pf1N1B4_1925 0.85 -0.0 2,201,997 - Pf1N1B4_1925 0.85 -0.2 2,201,997 - Pf1N1B4_1925 0.85 -1.6 2,201,997 - Pf1N1B4_1925 0.85 -0.0 2,201,997 - Pf1N1B4_1925 0.85 -0.0 2,201,997 - Pf1N1B4_1925 0.85 -1.7 2,201,997 - Pf1N1B4_1925 0.85 -0.4 2,202,001 - Pf1N1B4_1925 0.85 -0.0 2,202,060 + -0.0 2,202,060 + -0.0 2,202,060 + -2.3 2,202,061 - +1.0 2,202,061 - -0.9 2,202,127 - -0.0 2,202,142 + -0.0
Or see this region's nucleotide sequence