Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00100 and PGA1_65p00110 are separated by 269 nucleotides PGA1_65p00110 and PGA1_65p00120 are separated by 8 nucleotides PGA1_65p00120 and PGA1_65p00130 are separated by 8 nucleotides
PGA1_65p00100: PGA1_65p00100 - Thioesterase domains of type I polyketide synthases or non-ribosomal peptide synthetases, at 15,434 to 15,985
_65p00100
PGA1_65p00110: PGA1_65p00110 - putative amino-acid ABC transporter, periplasmic solute-binding protein, at 16,255 to 17,031
_65p00110
PGA1_65p00120: PGA1_65p00120 - putative amino-acid ABC transporter, permease protein, at 17,040 to 17,714
_65p00120
PGA1_65p00130: PGA1_65p00130 - putative amino-acid ABC transporter, ATP-binding protein, at 17,723 to 18,478
_65p00130
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-1
0
1
2 at 15.646 kb on + strand, within PGA1_65p00100 at 16.186 kb on - strand at 16.197 kb on - strand at 16.230 kb on - strand at 16.511 kb on - strand, within PGA1_65p00110 at 16.740 kb on + strand, within PGA1_65p00110 at 16.770 kb on - strand, within PGA1_65p00110 at 16.775 kb on - strand, within PGA1_65p00110 at 16.881 kb on + strand, within PGA1_65p00110 at 16.912 kb on - strand, within PGA1_65p00110 at 16.931 kb on - strand, within PGA1_65p00110 at 17.274 kb on - strand, within PGA1_65p00120 at 17.436 kb on - strand, within PGA1_65p00120 at 17.519 kb on + strand, within PGA1_65p00120 at 17.657 kb on + strand at 17.713 kb on - strand at 17.738 kb on - strand at 17.851 kb on + strand, within PGA1_65p00130 at 17.978 kb on - strand, within PGA1_65p00130
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 15,646 + PGA1_65p00100 0.38 +1.0 16,186 - +0.9 16,197 - +1.0 16,230 - -0.3 16,511 - PGA1_65p00110 0.33 +0.4 16,740 + PGA1_65p00110 0.62 +1.0 16,770 - PGA1_65p00110 0.66 +0.7 16,775 - PGA1_65p00110 0.67 +0.7 16,881 + PGA1_65p00110 0.81 +0.6 16,912 - PGA1_65p00110 0.85 +0.1 16,931 - PGA1_65p00110 0.87 -0.8 17,274 - PGA1_65p00120 0.35 -0.7 17,436 - PGA1_65p00120 0.59 +0.7 17,519 + PGA1_65p00120 0.71 -0.0 17,657 + +2.3 17,713 - +0.7 17,738 - -0.8 17,851 + PGA1_65p00130 0.17 +1.4 17,978 - PGA1_65p00130 0.34 -0.4
Or see this region's nucleotide sequence