Experiment: BHIS with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4331 and BT4332 are separated by 54 nucleotides BT4332 and BT4333 are separated by 6 nucleotides BT4333 and BT4334 are separated by 122 nucleotides
BT4331: BT4331 - conserved hypothetical protein (NCBI ptt file), at 5,704,334 to 5,705,512
BT4331
BT4332: BT4332 - conserved hypothetical protein (NCBI ptt file), at 5,705,567 to 5,706,217
BT4332
BT4333: BT4333 - hypothetical protein (NCBI ptt file), at 5,706,224 to 5,706,883
BT4333
BT4334: BT4334 - FtsK/SpoIIIE family protein (NCBI ptt file), at 5,707,006 to 5,709,501
BT4334
Position (kb) 5706
5707 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 5705.258 kb on - strand, within BT4331 at 5705.258 kb on - strand, within BT4331 at 5705.258 kb on - strand, within BT4331 at 5705.258 kb on - strand, within BT4331 at 5705.263 kb on - strand, within BT4331 at 5705.278 kb on + strand, within BT4331 at 5705.279 kb on - strand, within BT4331 at 5705.279 kb on - strand, within BT4331 at 5705.279 kb on - strand, within BT4331 at 5705.331 kb on - strand, within BT4331 at 5705.331 kb on - strand, within BT4331 at 5705.368 kb on + strand, within BT4331 at 5705.388 kb on + strand, within BT4331 at 5705.388 kb on + strand, within BT4331 at 5705.391 kb on - strand, within BT4331 at 5705.391 kb on - strand, within BT4331 at 5705.391 kb on - strand, within BT4331 at 5705.506 kb on + strand at 5705.520 kb on - strand at 5705.527 kb on + strand at 5705.533 kb on - strand at 5705.539 kb on + strand at 5705.539 kb on + strand at 5705.540 kb on - strand at 5705.542 kb on + strand at 5705.543 kb on - strand at 5705.664 kb on + strand, within BT4332 at 5705.664 kb on + strand, within BT4332 at 5705.723 kb on + strand, within BT4332 at 5705.832 kb on + strand, within BT4332 at 5705.974 kb on + strand, within BT4332 at 5705.975 kb on - strand, within BT4332 at 5706.026 kb on + strand, within BT4332 at 5706.026 kb on + strand, within BT4332 at 5706.026 kb on + strand, within BT4332 at 5706.031 kb on + strand, within BT4332 at 5706.032 kb on - strand, within BT4332 at 5706.107 kb on - strand, within BT4332 at 5706.108 kb on - strand, within BT4332 at 5706.161 kb on + strand at 5706.182 kb on + strand at 5706.183 kb on - strand at 5706.209 kb on + strand at 5706.226 kb on - strand at 5706.309 kb on + strand, within BT4333 at 5706.332 kb on - strand, within BT4333 at 5706.376 kb on + strand, within BT4333 at 5706.410 kb on + strand, within BT4333 at 5706.505 kb on + strand, within BT4333 at 5706.517 kb on + strand, within BT4333 at 5706.576 kb on + strand, within BT4333 at 5706.583 kb on + strand, within BT4333 at 5706.606 kb on + strand, within BT4333 at 5706.606 kb on + strand, within BT4333 at 5706.606 kb on + strand, within BT4333 at 5706.606 kb on + strand, within BT4333 at 5706.837 kb on - strand at 5706.860 kb on + strand at 5706.910 kb on + strand at 5706.967 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM remove 5,705,258 - BT4331 0.78 -2.9 5,705,258 - BT4331 0.78 +0.5 5,705,258 - BT4331 0.78 -3.9 5,705,258 - BT4331 0.78 -1.6 5,705,263 - BT4331 0.79 -1.8 5,705,278 + BT4331 0.80 -3.5 5,705,279 - BT4331 0.80 -3.5 5,705,279 - BT4331 0.80 +2.0 5,705,279 - BT4331 0.80 +1.0 5,705,331 - BT4331 0.85 -0.8 5,705,331 - BT4331 0.85 +0.3 5,705,368 + BT4331 0.88 -2.7 5,705,388 + BT4331 0.89 -0.8 5,705,388 + BT4331 0.89 -3.7 5,705,391 - BT4331 0.90 -2.0 5,705,391 - BT4331 0.90 +0.8 5,705,391 - BT4331 0.90 +2.3 5,705,506 + -3.8 5,705,520 - -4.1 5,705,527 + -4.6 5,705,533 - -1.0 5,705,539 + +1.1 5,705,539 + -4.3 5,705,540 - -2.0 5,705,542 + -2.0 5,705,543 - -3.7 5,705,664 + BT4332 0.15 -5.2 5,705,664 + BT4332 0.15 -0.2 5,705,723 + BT4332 0.24 +1.2 5,705,832 + BT4332 0.41 -0.6 5,705,974 + BT4332 0.63 -2.9 5,705,975 - BT4332 0.63 -2.5 5,706,026 + BT4332 0.71 +1.0 5,706,026 + BT4332 0.71 -1.9 5,706,026 + BT4332 0.71 -3.4 5,706,031 + BT4332 0.71 +2.0 5,706,032 - BT4332 0.71 +2.3 5,706,107 - BT4332 0.83 -0.4 5,706,108 - BT4332 0.83 +0.2 5,706,161 + -2.8 5,706,182 + -0.0 5,706,183 - -1.0 5,706,209 + -0.0 5,706,226 - -2.8 5,706,309 + BT4333 0.13 -3.3 5,706,332 - BT4333 0.16 -1.6 5,706,376 + BT4333 0.23 -3.6 5,706,410 + BT4333 0.28 -3.2 5,706,505 + BT4333 0.43 -2.0 5,706,517 + BT4333 0.44 -0.0 5,706,576 + BT4333 0.53 +1.3 5,706,583 + BT4333 0.54 -1.7 5,706,606 + BT4333 0.58 -2.5 5,706,606 + BT4333 0.58 +1.6 5,706,606 + BT4333 0.58 -1.8 5,706,606 + BT4333 0.58 -3.7 5,706,837 - -0.4 5,706,860 + -3.6 5,706,910 + -5.8 5,706,967 + +1.0
Or see this region's nucleotide sequence