Experiment: BHIS with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4085 and BT4086 are separated by 29 nucleotides BT4086 and BT4087 are separated by 17 nucleotides
BT4085: BT4085 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,330,640 to 5,332,496
BT4085
BT4086: BT4086 - hypothetical protein (NCBI ptt file), at 5,332,526 to 5,332,978
BT4086
BT4087: BT4087 - hypothetical protein (NCBI ptt file), at 5,332,996 to 5,334,048
BT4087
Position (kb)
5332
5333 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 5331.529 kb on - strand, within BT4085 at 5331.537 kb on + strand, within BT4085 at 5331.589 kb on - strand, within BT4085 at 5331.613 kb on - strand, within BT4085 at 5331.616 kb on - strand, within BT4085 at 5331.674 kb on + strand, within BT4085 at 5331.675 kb on - strand, within BT4085 at 5331.684 kb on - strand, within BT4085 at 5331.736 kb on - strand, within BT4085 at 5331.857 kb on + strand, within BT4085 at 5331.862 kb on - strand, within BT4085 at 5331.874 kb on + strand, within BT4085 at 5331.885 kb on - strand, within BT4085 at 5331.885 kb on - strand, within BT4085 at 5331.885 kb on - strand, within BT4085 at 5331.964 kb on - strand, within BT4085 at 5331.965 kb on + strand, within BT4085 at 5331.966 kb on - strand, within BT4085 at 5332.047 kb on - strand, within BT4085 at 5332.103 kb on + strand, within BT4085 at 5332.138 kb on - strand, within BT4085 at 5332.139 kb on + strand, within BT4085 at 5332.139 kb on + strand, within BT4085 at 5332.139 kb on + strand, within BT4085 at 5332.140 kb on - strand, within BT4085 at 5332.140 kb on - strand, within BT4085 at 5332.140 kb on - strand, within BT4085 at 5332.145 kb on - strand, within BT4085 at 5332.175 kb on - strand, within BT4085 at 5332.175 kb on - strand, within BT4085 at 5332.298 kb on - strand, within BT4085 at 5332.298 kb on - strand, within BT4085 at 5332.298 kb on - strand, within BT4085 at 5332.328 kb on + strand at 5332.329 kb on - strand at 5332.331 kb on + strand at 5332.332 kb on - strand at 5332.407 kb on - strand at 5332.446 kb on - strand at 5332.446 kb on - strand at 5332.446 kb on - strand at 5332.546 kb on + strand at 5332.622 kb on + strand, within BT4086 at 5332.623 kb on - strand, within BT4086 at 5332.675 kb on - strand, within BT4086 at 5332.692 kb on + strand, within BT4086 at 5332.693 kb on - strand, within BT4086 at 5332.748 kb on + strand, within BT4086 at 5332.751 kb on - strand, within BT4086 at 5332.763 kb on + strand, within BT4086 at 5332.865 kb on - strand, within BT4086 at 5332.866 kb on + strand, within BT4086 at 5332.921 kb on + strand, within BT4086 at 5333.132 kb on - strand, within BT4087 at 5333.136 kb on + strand, within BT4087 at 5333.137 kb on - strand, within BT4087 at 5333.137 kb on - strand, within BT4087 at 5333.211 kb on + strand, within BT4087 at 5333.285 kb on - strand, within BT4087 at 5333.345 kb on - strand, within BT4087 at 5333.353 kb on - strand, within BT4087 at 5333.391 kb on - strand, within BT4087 at 5333.430 kb on + strand, within BT4087 at 5333.443 kb on + strand, within BT4087 at 5333.444 kb on - strand, within BT4087 at 5333.473 kb on - strand, within BT4087 at 5333.473 kb on - strand, within BT4087 at 5333.473 kb on - strand, within BT4087 at 5333.476 kb on - strand, within BT4087 at 5333.485 kb on - strand, within BT4087 at 5333.613 kb on - strand, within BT4087 at 5333.654 kb on - strand, within BT4087 at 5333.693 kb on - strand, within BT4087 at 5333.697 kb on - strand, within BT4087 at 5333.756 kb on - strand, within BT4087 at 5333.760 kb on - strand, within BT4087 at 5333.760 kb on - strand, within BT4087 at 5333.760 kb on - strand, within BT4087 at 5333.766 kb on + strand, within BT4087 at 5333.774 kb on - strand, within BT4087 at 5333.774 kb on - strand, within BT4087 at 5333.792 kb on + strand, within BT4087 at 5333.795 kb on - strand, within BT4087 at 5333.795 kb on - strand, within BT4087 at 5333.923 kb on + strand, within BT4087 at 5333.952 kb on - strand at 5333.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM remove 5,331,529 - BT4085 0.48 -0.5 5,331,537 + BT4085 0.48 +0.1 5,331,589 - BT4085 0.51 +2.1 5,331,613 - BT4085 0.52 +2.4 5,331,616 - BT4085 0.53 +1.9 5,331,674 + BT4085 0.56 -1.1 5,331,675 - BT4085 0.56 -3.1 5,331,684 - BT4085 0.56 +0.1 5,331,736 - BT4085 0.59 -0.9 5,331,857 + BT4085 0.66 +0.8 5,331,862 - BT4085 0.66 -1.9 5,331,874 + BT4085 0.66 -0.9 5,331,885 - BT4085 0.67 +0.6 5,331,885 - BT4085 0.67 -1.5 5,331,885 - BT4085 0.67 -0.9 5,331,964 - BT4085 0.71 +3.5 5,331,965 + BT4085 0.71 -2.5 5,331,966 - BT4085 0.71 -2.0 5,332,047 - BT4085 0.76 -2.8 5,332,103 + BT4085 0.79 +1.6 5,332,138 - BT4085 0.81 -0.9 5,332,139 + BT4085 0.81 +0.1 5,332,139 + BT4085 0.81 -1.6 5,332,139 + BT4085 0.81 +1.1 5,332,140 - BT4085 0.81 -2.5 5,332,140 - BT4085 0.81 -2.4 5,332,140 - BT4085 0.81 +2.9 5,332,145 - BT4085 0.81 +0.2 5,332,175 - BT4085 0.83 -3.8 5,332,175 - BT4085 0.83 +1.4 5,332,298 - BT4085 0.89 -2.8 5,332,298 - BT4085 0.89 -1.7 5,332,298 - BT4085 0.89 -1.4 5,332,328 + +2.0 5,332,329 - -1.5 5,332,331 + -3.3 5,332,332 - +2.9 5,332,407 - +0.6 5,332,446 - -3.4 5,332,446 - -2.7 5,332,446 - -1.4 5,332,546 + +0.0 5,332,622 + BT4086 0.21 +1.0 5,332,623 - BT4086 0.21 +0.7 5,332,675 - BT4086 0.33 +2.4 5,332,692 + BT4086 0.37 +0.0 5,332,693 - BT4086 0.37 -2.0 5,332,748 + BT4086 0.49 +1.9 5,332,751 - BT4086 0.50 +0.8 5,332,763 + BT4086 0.52 +1.0 5,332,865 - BT4086 0.75 +0.6 5,332,866 + BT4086 0.75 -3.1 5,332,921 + BT4086 0.87 +3.4 5,333,132 - BT4087 0.13 +3.0 5,333,136 + BT4087 0.13 -1.5 5,333,137 - BT4087 0.13 +5.7 5,333,137 - BT4087 0.13 -1.1 5,333,211 + BT4087 0.20 +1.4 5,333,285 - BT4087 0.27 -1.0 5,333,345 - BT4087 0.33 -0.7 5,333,353 - BT4087 0.34 +1.0 5,333,391 - BT4087 0.38 -0.1 5,333,430 + BT4087 0.41 +0.9 5,333,443 + BT4087 0.42 -0.5 5,333,444 - BT4087 0.43 +1.6 5,333,473 - BT4087 0.45 +2.0 5,333,473 - BT4087 0.45 +2.0 5,333,473 - BT4087 0.45 +1.0 5,333,476 - BT4087 0.46 +3.6 5,333,485 - BT4087 0.46 +1.0 5,333,613 - BT4087 0.59 +0.0 5,333,654 - BT4087 0.62 -0.4 5,333,693 - BT4087 0.66 -0.6 5,333,697 - BT4087 0.67 -0.9 5,333,756 - BT4087 0.72 +2.6 5,333,760 - BT4087 0.73 -2.0 5,333,760 - BT4087 0.73 -1.4 5,333,760 - BT4087 0.73 -1.7 5,333,766 + BT4087 0.73 +2.4 5,333,774 - BT4087 0.74 -0.5 5,333,774 - BT4087 0.74 -2.1 5,333,792 + BT4087 0.76 -0.4 5,333,795 - BT4087 0.76 +1.6 5,333,795 - BT4087 0.76 +2.4 5,333,923 + BT4087 0.88 -2.3 5,333,952 - +2.5 5,333,956 - -2.1
Or see this region's nucleotide sequence