Experiment: BHIS with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3837 and BT3838 are separated by 75 nucleotides BT3838 and BT3839 overlap by 4 nucleotides BT3839 and BT3840 are separated by 88 nucleotides BT3840 and BT3841 are separated by 398 nucleotides
BT3837: BT3837 - ABC transporter ATP-binding protein (NCBI ptt file), at 4,993,076 to 4,993,843
BT3837
BT3838: BT3838 - conserved hypothetical protein (NCBI ptt file), at 4,993,919 to 4,994,662
BT3838
BT3839: BT3839 - ABC transporter ATP-binding protein (NCBI ptt file), at 4,994,659 to 4,995,429
BT3839
BT3840: BT3840 - putative RNA-binding protein rbpA (NCBI ptt file), at 4,995,518 to 4,995,763
BT3840
BT3841: BT3841 - FKBP-type peptidyl-prolyl cis-transisomerase (trigger factor) (NCBI ptt file), at 4,996,162 to 4,997,517
BT3841
Position (kb)
4994
4995
4996 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4993.918 kb on + strand at 4993.918 kb on + strand at 4993.950 kb on - strand at 4993.950 kb on - strand at 4993.950 kb on - strand at 4993.951 kb on + strand at 4993.990 kb on + strand at 4994.194 kb on + strand, within BT3838 at 4994.459 kb on - strand, within BT3838 at 4994.553 kb on + strand, within BT3838 at 4994.588 kb on - strand at 4994.716 kb on - strand at 4994.836 kb on + strand, within BT3839 at 4995.179 kb on + strand, within BT3839 at 4995.220 kb on - strand, within BT3839 at 4995.304 kb on - strand, within BT3839 at 4995.391 kb on - strand at 4995.417 kb on + strand at 4995.417 kb on + strand at 4995.418 kb on - strand at 4995.418 kb on - strand at 4995.418 kb on - strand at 4995.442 kb on + strand at 4995.442 kb on + strand at 4995.453 kb on - strand at 4995.520 kb on - strand at 4995.545 kb on + strand, within BT3840 at 4995.545 kb on + strand, within BT3840 at 4995.554 kb on - strand, within BT3840 at 4995.669 kb on - strand, within BT3840 at 4995.735 kb on + strand, within BT3840 at 4995.784 kb on - strand at 4995.821 kb on - strand at 4995.823 kb on + strand at 4995.824 kb on - strand at 4996.042 kb on - strand at 4996.042 kb on - strand at 4996.193 kb on + strand at 4996.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM remove 4,993,918 + -2.3 4,993,918 + +4.0 4,993,950 - -0.0 4,993,950 - -1.3 4,993,950 - +0.5 4,993,951 + +1.3 4,993,990 + -0.0 4,994,194 + BT3838 0.37 +2.6 4,994,459 - BT3838 0.73 +3.2 4,994,553 + BT3838 0.85 +1.3 4,994,588 - -0.0 4,994,716 - -0.0 4,994,836 + BT3839 0.23 +1.6 4,995,179 + BT3839 0.67 +1.6 4,995,220 - BT3839 0.73 +0.8 4,995,304 - BT3839 0.84 +3.3 4,995,391 - +0.8 4,995,417 + -3.8 4,995,417 + +3.2 4,995,418 - -3.0 4,995,418 - -0.1 4,995,418 - +0.8 4,995,442 + +1.1 4,995,442 + -2.1 4,995,453 - -2.9 4,995,520 - +1.2 4,995,545 + BT3840 0.11 -0.7 4,995,545 + BT3840 0.11 -0.7 4,995,554 - BT3840 0.15 -2.3 4,995,669 - BT3840 0.61 +3.2 4,995,735 + BT3840 0.88 -0.0 4,995,784 - +1.0 4,995,821 - -0.0 4,995,823 + +0.6 4,995,824 - -1.5 4,996,042 - -0.0 4,996,042 - -1.7 4,996,193 + -1.0 4,996,206 + -1.0
Or see this region's nucleotide sequence