Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2057

Experiment: BHIS with 5-Fluorouracil 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttRNA-Ser and BT2056 are separated by 120 nucleotidesBT2056 and BT2057 overlap by 7 nucleotidesBT2057 and BT2058 are separated by 15 nucleotides BT_t34: tRNA-Ser - tRNA-Ser (RefSeq), at 2,575,512 to 2,575,599 tRNA-Ser BT2056: BT2056 - conserved hypothetical protein (NCBI ptt file), at 2,575,720 to 2,576,496 BT2056 BT2057: BT2057 - hypothetical protein (NCBI ptt file), at 2,576,490 to 2,577,206 BT2057 BT2058: BT2058 - putative isoprenyl synthetase (NCBI ptt file), at 2,577,222 to 2,578,196 BT2058 Position (kb) 2576 2577 2578Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2575.553 kb on - strand, within tRNA-Serat 2575.681 kb on - strandat 2575.681 kb on - strandat 2575.699 kb on + strandat 2575.818 kb on + strand, within BT2056at 2575.819 kb on - strand, within BT2056at 2576.079 kb on + strand, within BT2056at 2576.082 kb on - strand, within BT2056at 2576.083 kb on + strand, within BT2056at 2576.083 kb on + strand, within BT2056at 2576.083 kb on + strand, within BT2056at 2576.085 kb on + strand, within BT2056at 2576.087 kb on + strand, within BT2056at 2576.127 kb on + strand, within BT2056at 2576.127 kb on + strand, within BT2056at 2576.227 kb on + strand, within BT2056at 2576.235 kb on + strand, within BT2056at 2576.236 kb on - strand, within BT2056at 2576.301 kb on + strand, within BT2056at 2576.394 kb on + strand, within BT2056at 2576.400 kb on + strand, within BT2056at 2576.484 kb on + strandat 2576.484 kb on + strandat 2576.488 kb on + strandat 2576.489 kb on - strandat 2576.539 kb on + strandat 2576.543 kb on - strandat 2576.719 kb on + strand, within BT2057at 2576.719 kb on + strand, within BT2057at 2576.740 kb on + strand, within BT2057at 2576.752 kb on - strand, within BT2057at 2576.773 kb on + strand, within BT2057at 2576.893 kb on + strand, within BT2057at 2576.947 kb on - strand, within BT2057at 2576.947 kb on - strand, within BT2057at 2576.966 kb on + strand, within BT2057at 2576.968 kb on + strand, within BT2057at 2576.968 kb on + strand, within BT2057at 2576.968 kb on + strand, within BT2057at 2576.969 kb on - strand, within BT2057at 2577.038 kb on + strand, within BT2057at 2577.125 kb on + strand, within BT2057at 2577.128 kb on - strand, within BT2057at 2577.165 kb on + strandat 2577.200 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM
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2,575,553 - tRNA-Ser BT_t34 0.47 -2.7
2,575,681 - +0.1
2,575,681 - +1.7
2,575,699 + +2.3
2,575,818 + BT2056 0.13 -2.7
2,575,819 - BT2056 0.13 +2.1
2,576,079 + BT2056 0.46 +0.1
2,576,082 - BT2056 0.47 +0.8
2,576,083 + BT2056 0.47 -2.5
2,576,083 + BT2056 0.47 -0.2
2,576,083 + BT2056 0.47 -1.5
2,576,085 + BT2056 0.47 +1.1
2,576,087 + BT2056 0.47 +1.7
2,576,127 + BT2056 0.52 +0.1
2,576,127 + BT2056 0.52 -1.3
2,576,227 + BT2056 0.65 -1.2
2,576,235 + BT2056 0.66 +0.1
2,576,236 - BT2056 0.66 -1.6
2,576,301 + BT2056 0.75 +0.4
2,576,394 + BT2056 0.87 -1.5
2,576,400 + BT2056 0.88 -1.1
2,576,484 + -1.9
2,576,484 + -2.9
2,576,488 + -2.6
2,576,489 - +2.7
2,576,539 + -0.9
2,576,543 - -0.5
2,576,719 + BT2057 0.32 +2.8
2,576,719 + BT2057 0.32 +2.0
2,576,740 + BT2057 0.35 -1.9
2,576,752 - BT2057 0.37 +1.4
2,576,773 + BT2057 0.39 +1.1
2,576,893 + BT2057 0.56 -0.3
2,576,947 - BT2057 0.64 +2.4
2,576,947 - BT2057 0.64 -1.2
2,576,966 + BT2057 0.66 -1.3
2,576,968 + BT2057 0.67 -3.4
2,576,968 + BT2057 0.67 -0.9
2,576,968 + BT2057 0.67 +3.8
2,576,969 - BT2057 0.67 +0.1
2,577,038 + BT2057 0.76 +2.7
2,577,125 + BT2057 0.89 +3.6
2,577,128 - BT2057 0.89 -1.5
2,577,165 + +4.5
2,577,200 - +0.1

Or see this region's nucleotide sequence