Experiment: BHIS with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1778 and BT1779 are separated by 50 nucleotides BT1779 and BT1780 are separated by 30 nucleotides
BT1778: BT1778 - beta-glucosidase (gentiobiase) (NCBI ptt file), at 2,217,682 to 2,220,243
BT1778
BT1779: BT1779 - sialic acid-specific 9-O-acetylesterase (NCBI ptt file), at 2,220,294 to 2,222,012
BT1779
BT1780: BT1780 - periplasmic beta-glucosidase precursor (NCBI ptt file), at 2,222,043 to 2,224,895
BT1780
Position (kb)
2220
2221
2222
2223 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2219.316 kb on - strand, within BT1778 at 2219.316 kb on - strand, within BT1778 at 2219.390 kb on + strand, within BT1778 at 2219.394 kb on + strand, within BT1778 at 2219.523 kb on + strand, within BT1778 at 2219.526 kb on - strand, within BT1778 at 2219.526 kb on - strand, within BT1778 at 2219.691 kb on - strand, within BT1778 at 2219.765 kb on + strand, within BT1778 at 2219.995 kb on + strand at 2219.995 kb on + strand at 2219.995 kb on + strand at 2220.026 kb on + strand at 2220.026 kb on + strand at 2220.029 kb on + strand at 2220.155 kb on + strand at 2220.158 kb on - strand at 2220.222 kb on + strand at 2220.258 kb on + strand at 2220.273 kb on + strand at 2220.399 kb on + strand at 2220.467 kb on + strand, within BT1779 at 2220.623 kb on + strand, within BT1779 at 2220.625 kb on + strand, within BT1779 at 2220.659 kb on - strand, within BT1779 at 2220.703 kb on + strand, within BT1779 at 2220.703 kb on + strand, within BT1779 at 2220.704 kb on - strand, within BT1779 at 2220.707 kb on + strand, within BT1779 at 2220.738 kb on + strand, within BT1779 at 2220.738 kb on + strand, within BT1779 at 2220.739 kb on - strand, within BT1779 at 2220.873 kb on + strand, within BT1779 at 2220.943 kb on + strand, within BT1779 at 2221.002 kb on - strand, within BT1779 at 2221.055 kb on + strand, within BT1779 at 2221.056 kb on - strand, within BT1779 at 2221.250 kb on - strand, within BT1779 at 2221.304 kb on - strand, within BT1779 at 2221.630 kb on + strand, within BT1779 at 2221.647 kb on + strand, within BT1779 at 2221.686 kb on + strand, within BT1779 at 2221.839 kb on + strand, within BT1779 at 2221.936 kb on - strand at 2222.041 kb on + strand at 2222.213 kb on + strand at 2222.220 kb on - strand at 2222.271 kb on + strand at 2222.271 kb on + strand at 2222.315 kb on + strand at 2222.316 kb on - strand at 2222.317 kb on + strand at 2222.318 kb on - strand at 2222.321 kb on + strand at 2222.354 kb on + strand, within BT1780 at 2222.361 kb on + strand, within BT1780 at 2222.361 kb on + strand, within BT1780 at 2222.361 kb on + strand, within BT1780 at 2222.361 kb on + strand, within BT1780 at 2222.361 kb on + strand, within BT1780 at 2222.366 kb on + strand, within BT1780 at 2222.367 kb on - strand, within BT1780 at 2222.475 kb on - strand, within BT1780 at 2222.480 kb on - strand, within BT1780 at 2222.578 kb on + strand, within BT1780 at 2222.578 kb on + strand, within BT1780 at 2222.578 kb on + strand, within BT1780 at 2222.579 kb on - strand, within BT1780 at 2222.593 kb on + strand, within BT1780 at 2222.593 kb on + strand, within BT1780 at 2222.595 kb on + strand, within BT1780 at 2222.690 kb on + strand, within BT1780 at 2222.757 kb on + strand, within BT1780 at 2222.759 kb on + strand, within BT1780 at 2222.762 kb on - strand, within BT1780 at 2222.794 kb on + strand, within BT1780 at 2222.858 kb on - strand, within BT1780 at 2222.858 kb on - strand, within BT1780 at 2222.871 kb on + strand, within BT1780 at 2222.871 kb on + strand, within BT1780 at 2222.872 kb on - strand, within BT1780 at 2222.924 kb on + strand, within BT1780 at 2222.954 kb on + strand, within BT1780
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM remove 2,219,316 - BT1778 0.64 +0.1 2,219,316 - BT1778 0.64 -4.6 2,219,390 + BT1778 0.67 -0.2 2,219,394 + BT1778 0.67 +2.1 2,219,523 + BT1778 0.72 -1.5 2,219,526 - BT1778 0.72 +0.1 2,219,526 - BT1778 0.72 +2.1 2,219,691 - BT1778 0.78 -2.3 2,219,765 + BT1778 0.81 +2.9 2,219,995 + +0.1 2,219,995 + -2.2 2,219,995 + -0.4 2,220,026 + +1.9 2,220,026 + -2.3 2,220,029 + -1.5 2,220,155 + -0.4 2,220,158 - +0.7 2,220,222 + -1.4 2,220,258 + -1.3 2,220,273 + +2.7 2,220,399 + +0.5 2,220,467 + BT1779 0.10 -1.5 2,220,623 + BT1779 0.19 +0.5 2,220,625 + BT1779 0.19 -2.5 2,220,659 - BT1779 0.21 -2.1 2,220,703 + BT1779 0.24 -1.1 2,220,703 + BT1779 0.24 -1.9 2,220,704 - BT1779 0.24 -1.5 2,220,707 + BT1779 0.24 +0.1 2,220,738 + BT1779 0.26 -0.9 2,220,738 + BT1779 0.26 -1.5 2,220,739 - BT1779 0.26 -1.7 2,220,873 + BT1779 0.34 -2.0 2,220,943 + BT1779 0.38 -2.3 2,221,002 - BT1779 0.41 -1.5 2,221,055 + BT1779 0.44 +0.1 2,221,056 - BT1779 0.44 +1.1 2,221,250 - BT1779 0.56 -0.9 2,221,304 - BT1779 0.59 -1.9 2,221,630 + BT1779 0.78 -1.9 2,221,647 + BT1779 0.79 -1.9 2,221,686 + BT1779 0.81 +2.7 2,221,839 + BT1779 0.90 +0.1 2,221,936 - -0.8 2,222,041 + -2.7 2,222,213 + -0.9 2,222,220 - +2.9 2,222,271 + +0.1 2,222,271 + -2.5 2,222,315 + -0.5 2,222,316 - -0.9 2,222,317 + -0.9 2,222,318 - -1.5 2,222,321 + +0.5 2,222,354 + BT1780 0.11 -0.7 2,222,361 + BT1780 0.11 +0.5 2,222,361 + BT1780 0.11 -1.9 2,222,361 + BT1780 0.11 -0.9 2,222,361 + BT1780 0.11 -1.7 2,222,361 + BT1780 0.11 +1.6 2,222,366 + BT1780 0.11 -0.9 2,222,367 - BT1780 0.11 +0.1 2,222,475 - BT1780 0.15 +2.4 2,222,480 - BT1780 0.15 +0.1 2,222,578 + BT1780 0.19 +0.1 2,222,578 + BT1780 0.19 -1.1 2,222,578 + BT1780 0.19 +1.9 2,222,579 - BT1780 0.19 +0.4 2,222,593 + BT1780 0.19 +0.4 2,222,593 + BT1780 0.19 +2.1 2,222,595 + BT1780 0.19 +1.9 2,222,690 + BT1780 0.23 -1.7 2,222,757 + BT1780 0.25 +0.8 2,222,759 + BT1780 0.25 +2.7 2,222,762 - BT1780 0.25 -1.3 2,222,794 + BT1780 0.26 +2.1 2,222,858 - BT1780 0.29 -1.5 2,222,858 - BT1780 0.29 +3.7 2,222,871 + BT1780 0.29 +0.1 2,222,871 + BT1780 0.29 +0.1 2,222,872 - BT1780 0.29 +0.1 2,222,924 + BT1780 0.31 -3.7 2,222,954 + BT1780 0.32 +1.5
Or see this region's nucleotide sequence