Experiment: BHIS with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1503 and BT1504 are separated by 281 nucleotides BT1504 and BT1505 are separated by 9 nucleotides
BT1503: BT1503 - integrase (NCBI ptt file), at 1,855,012 to 1,856,022
BT1503
BT1504: BT1504 - hypothetical protein (NCBI ptt file), at 1,856,304 to 1,856,567
BT1504
BT1505: BT1505 - hypothetical protein (NCBI ptt file), at 1,856,577 to 1,857,665
BT1505
Position (kb)
1856
1857 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1855.344 kb on - strand, within BT1503 at 1855.356 kb on + strand, within BT1503 at 1855.382 kb on - strand, within BT1503 at 1855.384 kb on - strand, within BT1503 at 1855.384 kb on - strand, within BT1503 at 1855.816 kb on + strand, within BT1503 at 1855.960 kb on + strand at 1856.022 kb on + strand at 1856.024 kb on + strand at 1856.024 kb on + strand at 1856.082 kb on + strand at 1856.108 kb on - strand at 1856.195 kb on - strand at 1856.203 kb on - strand at 1856.203 kb on - strand at 1856.203 kb on - strand at 1856.348 kb on + strand, within BT1504 at 1856.376 kb on - strand, within BT1504 at 1856.428 kb on + strand, within BT1504 at 1856.428 kb on + strand, within BT1504 at 1856.541 kb on - strand at 1856.589 kb on - strand at 1856.594 kb on + strand at 1856.595 kb on - strand at 1856.646 kb on - strand at 1856.768 kb on - strand, within BT1505 at 1857.270 kb on + strand, within BT1505 at 1857.329 kb on + strand, within BT1505 at 1857.352 kb on - strand, within BT1505 at 1857.371 kb on + strand, within BT1505 at 1857.473 kb on + strand, within BT1505 at 1857.551 kb on + strand, within BT1505
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM remove 1,855,344 - BT1503 0.33 -1.1 1,855,356 + BT1503 0.34 -1.9 1,855,382 - BT1503 0.37 +3.1 1,855,384 - BT1503 0.37 -1.1 1,855,384 - BT1503 0.37 -0.6 1,855,816 + BT1503 0.80 +1.4 1,855,960 + +2.5 1,856,022 + -2.7 1,856,024 + -3.1 1,856,024 + +0.9 1,856,082 + +1.9 1,856,108 - -1.7 1,856,195 - -0.1 1,856,203 - -0.4 1,856,203 - -1.1 1,856,203 - -0.7 1,856,348 + BT1504 0.17 -2.1 1,856,376 - BT1504 0.27 -2.9 1,856,428 + BT1504 0.47 +1.7 1,856,428 + BT1504 0.47 -1.1 1,856,541 - +0.9 1,856,589 - +1.9 1,856,594 + +2.8 1,856,595 - -1.1 1,856,646 - -0.6 1,856,768 - BT1505 0.18 -2.4 1,857,270 + BT1505 0.64 -0.1 1,857,329 + BT1505 0.69 -0.1 1,857,352 - BT1505 0.71 -0.1 1,857,371 + BT1505 0.73 -0.9 1,857,473 + BT1505 0.82 -1.3 1,857,551 + BT1505 0.89 +2.3
Or see this region's nucleotide sequence