Experiment: BHIS with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1068 and BT1069 are separated by 359 nucleotides BT1069 and BT1070 overlap by 4 nucleotides BT1070 and BT1071 are separated by 3 nucleotides
BT1068: BT1068 - hypothetical protein (NCBI ptt file), at 1,356,084 to 1,356,428
BT1068
BT1069: BT1069 - conserved hypothetical protein, putative transcriptional regulator (NCBI ptt file), at 1,356,788 to 1,357,330
BT1069
BT1070: BT1070 - conserved hypothetical protein (NCBI ptt file), at 1,357,327 to 1,358,631
BT1070
BT1071: BT1071 - hypothetical protein (NCBI ptt file), at 1,358,635 to 1,359,042
BT1071
Position (kb)
1357
1358
1359 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1356.329 kb on + strand, within BT1068 at 1356.333 kb on - strand, within BT1068 at 1356.364 kb on - strand, within BT1068 at 1356.366 kb on + strand, within BT1068 at 1356.369 kb on - strand, within BT1068 at 1356.443 kb on + strand at 1356.471 kb on + strand at 1356.706 kb on + strand at 1356.758 kb on + strand at 1356.759 kb on - strand at 1356.763 kb on + strand at 1356.763 kb on + strand at 1356.763 kb on + strand at 1356.965 kb on + strand, within BT1069 at 1356.991 kb on + strand, within BT1069 at 1357.024 kb on + strand, within BT1069 at 1357.121 kb on - strand, within BT1069 at 1357.121 kb on - strand, within BT1069 at 1357.149 kb on + strand, within BT1069 at 1357.149 kb on + strand at 1357.150 kb on - strand, within BT1069 at 1357.154 kb on - strand, within BT1069 at 1357.192 kb on + strand, within BT1069 at 1357.271 kb on + strand, within BT1069 at 1357.272 kb on - strand, within BT1069 at 1357.337 kb on - strand at 1357.345 kb on - strand at 1357.345 kb on - strand at 1357.407 kb on - strand at 1357.440 kb on + strand at 1357.446 kb on + strand at 1357.447 kb on - strand at 1357.499 kb on + strand, within BT1070 at 1357.524 kb on - strand, within BT1070 at 1357.683 kb on + strand, within BT1070 at 1357.702 kb on - strand, within BT1070 at 1357.770 kb on - strand, within BT1070 at 1357.770 kb on - strand, within BT1070 at 1357.770 kb on - strand, within BT1070 at 1357.920 kb on - strand, within BT1070 at 1357.931 kb on + strand, within BT1070 at 1357.943 kb on + strand, within BT1070 at 1358.048 kb on + strand, within BT1070 at 1358.052 kb on + strand, within BT1070 at 1358.057 kb on + strand, within BT1070 at 1358.057 kb on + strand, within BT1070 at 1358.085 kb on + strand, within BT1070 at 1358.328 kb on + strand, within BT1070 at 1358.360 kb on - strand, within BT1070 at 1358.360 kb on - strand, within BT1070 at 1358.541 kb on - strand at 1358.655 kb on - strand at 1358.754 kb on + strand, within BT1071 at 1358.822 kb on - strand, within BT1071 at 1358.875 kb on - strand, within BT1071 at 1358.947 kb on - strand at 1358.960 kb on - strand, within BT1071 at 1359.032 kb on - strand at 1359.148 kb on + strand at 1359.193 kb on + strand at 1359.217 kb on + strand at 1359.224 kb on + strand at 1359.330 kb on - strand at 1359.410 kb on + strand at 1359.465 kb on - strand at 1359.510 kb on + strand at 1359.510 kb on + strand at 1359.557 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM remove 1,356,329 + BT1068 0.71 +0.1 1,356,333 - BT1068 0.72 -3.5 1,356,364 - BT1068 0.81 -2.7 1,356,366 + BT1068 0.82 -1.9 1,356,369 - BT1068 0.83 -4.0 1,356,443 + -0.8 1,356,471 + +1.5 1,356,706 + -0.3 1,356,758 + -1.3 1,356,759 - +2.5 1,356,763 + -1.2 1,356,763 + +0.1 1,356,763 + +1.9 1,356,965 + BT1069 0.33 +2.5 1,356,991 + BT1069 0.37 -0.0 1,357,024 + BT1069 0.43 +0.7 1,357,121 - BT1069 0.61 +2.3 1,357,121 - BT1069 0.61 -1.3 1,357,149 + BT1069 0.66 +0.7 1,357,149 + +1.3 1,357,150 - BT1069 0.67 +1.9 1,357,154 - BT1069 0.67 -1.3 1,357,192 + BT1069 0.74 +1.7 1,357,271 + BT1069 0.89 +1.3 1,357,272 - BT1069 0.89 +1.9 1,357,337 - -0.9 1,357,345 - +0.1 1,357,345 - +1.6 1,357,407 - -0.9 1,357,440 + +0.7 1,357,446 + +1.8 1,357,447 - +0.7 1,357,499 + BT1070 0.13 +0.5 1,357,524 - BT1070 0.15 -0.8 1,357,683 + BT1070 0.27 +1.7 1,357,702 - BT1070 0.29 -0.3 1,357,770 - BT1070 0.34 +1.7 1,357,770 - BT1070 0.34 -0.3 1,357,770 - BT1070 0.34 +2.5 1,357,920 - BT1070 0.45 +1.4 1,357,931 + BT1070 0.46 +0.4 1,357,943 + BT1070 0.47 +2.3 1,358,048 + BT1070 0.55 +2.0 1,358,052 + BT1070 0.56 -0.3 1,358,057 + BT1070 0.56 -0.3 1,358,057 + BT1070 0.56 +1.7 1,358,085 + BT1070 0.58 +1.3 1,358,328 + BT1070 0.77 -1.3 1,358,360 - BT1070 0.79 -0.3 1,358,360 - BT1070 0.79 +1.7 1,358,541 - -0.3 1,358,655 - +0.1 1,358,754 + BT1071 0.29 -0.9 1,358,822 - BT1071 0.46 -3.0 1,358,875 - BT1071 0.59 -5.1 1,358,947 - -1.9 1,358,960 - BT1071 0.80 -1.9 1,359,032 - -3.1 1,359,148 + +1.2 1,359,193 + +0.7 1,359,217 + +0.7 1,359,224 + +0.5 1,359,330 - -2.3 1,359,410 + +2.0 1,359,465 - +1.7 1,359,510 + +1.3 1,359,510 + -0.3 1,359,557 + +1.7
Or see this region's nucleotide sequence