Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1070

Experiment: BHIS with 5-Fluorouracil 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1068 and BT1069 are separated by 359 nucleotidesBT1069 and BT1070 overlap by 4 nucleotidesBT1070 and BT1071 are separated by 3 nucleotides BT1068: BT1068 - hypothetical protein (NCBI ptt file), at 1,356,084 to 1,356,428 BT1068 BT1069: BT1069 - conserved hypothetical protein, putative transcriptional regulator (NCBI ptt file), at 1,356,788 to 1,357,330 BT1069 BT1070: BT1070 - conserved hypothetical protein (NCBI ptt file), at 1,357,327 to 1,358,631 BT1070 BT1071: BT1071 - hypothetical protein (NCBI ptt file), at 1,358,635 to 1,359,042 BT1071 Position (kb) 1357 1358 1359Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1356.329 kb on + strand, within BT1068at 1356.333 kb on - strand, within BT1068at 1356.364 kb on - strand, within BT1068at 1356.366 kb on + strand, within BT1068at 1356.369 kb on - strand, within BT1068at 1356.443 kb on + strandat 1356.471 kb on + strandat 1356.706 kb on + strandat 1356.758 kb on + strandat 1356.759 kb on - strandat 1356.763 kb on + strandat 1356.763 kb on + strandat 1356.763 kb on + strandat 1356.965 kb on + strand, within BT1069at 1356.991 kb on + strand, within BT1069at 1357.024 kb on + strand, within BT1069at 1357.121 kb on - strand, within BT1069at 1357.121 kb on - strand, within BT1069at 1357.149 kb on + strand, within BT1069at 1357.149 kb on + strandat 1357.150 kb on - strand, within BT1069at 1357.154 kb on - strand, within BT1069at 1357.192 kb on + strand, within BT1069at 1357.271 kb on + strand, within BT1069at 1357.272 kb on - strand, within BT1069at 1357.337 kb on - strandat 1357.345 kb on - strandat 1357.345 kb on - strandat 1357.407 kb on - strandat 1357.440 kb on + strandat 1357.446 kb on + strandat 1357.447 kb on - strandat 1357.499 kb on + strand, within BT1070at 1357.524 kb on - strand, within BT1070at 1357.683 kb on + strand, within BT1070at 1357.702 kb on - strand, within BT1070at 1357.770 kb on - strand, within BT1070at 1357.770 kb on - strand, within BT1070at 1357.770 kb on - strand, within BT1070at 1357.920 kb on - strand, within BT1070at 1357.931 kb on + strand, within BT1070at 1357.943 kb on + strand, within BT1070at 1358.048 kb on + strand, within BT1070at 1358.052 kb on + strand, within BT1070at 1358.057 kb on + strand, within BT1070at 1358.057 kb on + strand, within BT1070at 1358.085 kb on + strand, within BT1070at 1358.328 kb on + strand, within BT1070at 1358.360 kb on - strand, within BT1070at 1358.360 kb on - strand, within BT1070at 1358.541 kb on - strandat 1358.655 kb on - strandat 1358.754 kb on + strand, within BT1071at 1358.822 kb on - strand, within BT1071at 1358.875 kb on - strand, within BT1071at 1358.947 kb on - strandat 1358.960 kb on - strand, within BT1071at 1359.032 kb on - strandat 1359.148 kb on + strandat 1359.193 kb on + strandat 1359.217 kb on + strandat 1359.224 kb on + strandat 1359.330 kb on - strandat 1359.410 kb on + strandat 1359.465 kb on - strandat 1359.510 kb on + strandat 1359.510 kb on + strandat 1359.557 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM
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1,356,329 + BT1068 0.71 +0.1
1,356,333 - BT1068 0.72 -3.5
1,356,364 - BT1068 0.81 -2.7
1,356,366 + BT1068 0.82 -1.9
1,356,369 - BT1068 0.83 -4.0
1,356,443 + -0.8
1,356,471 + +1.5
1,356,706 + -0.3
1,356,758 + -1.3
1,356,759 - +2.5
1,356,763 + -1.2
1,356,763 + +0.1
1,356,763 + +1.9
1,356,965 + BT1069 0.33 +2.5
1,356,991 + BT1069 0.37 -0.0
1,357,024 + BT1069 0.43 +0.7
1,357,121 - BT1069 0.61 +2.3
1,357,121 - BT1069 0.61 -1.3
1,357,149 + BT1069 0.66 +0.7
1,357,149 + +1.3
1,357,150 - BT1069 0.67 +1.9
1,357,154 - BT1069 0.67 -1.3
1,357,192 + BT1069 0.74 +1.7
1,357,271 + BT1069 0.89 +1.3
1,357,272 - BT1069 0.89 +1.9
1,357,337 - -0.9
1,357,345 - +0.1
1,357,345 - +1.6
1,357,407 - -0.9
1,357,440 + +0.7
1,357,446 + +1.8
1,357,447 - +0.7
1,357,499 + BT1070 0.13 +0.5
1,357,524 - BT1070 0.15 -0.8
1,357,683 + BT1070 0.27 +1.7
1,357,702 - BT1070 0.29 -0.3
1,357,770 - BT1070 0.34 +1.7
1,357,770 - BT1070 0.34 -0.3
1,357,770 - BT1070 0.34 +2.5
1,357,920 - BT1070 0.45 +1.4
1,357,931 + BT1070 0.46 +0.4
1,357,943 + BT1070 0.47 +2.3
1,358,048 + BT1070 0.55 +2.0
1,358,052 + BT1070 0.56 -0.3
1,358,057 + BT1070 0.56 -0.3
1,358,057 + BT1070 0.56 +1.7
1,358,085 + BT1070 0.58 +1.3
1,358,328 + BT1070 0.77 -1.3
1,358,360 - BT1070 0.79 -0.3
1,358,360 - BT1070 0.79 +1.7
1,358,541 - -0.3
1,358,655 - +0.1
1,358,754 + BT1071 0.29 -0.9
1,358,822 - BT1071 0.46 -3.0
1,358,875 - BT1071 0.59 -5.1
1,358,947 - -1.9
1,358,960 - BT1071 0.80 -1.9
1,359,032 - -3.1
1,359,148 + +1.2
1,359,193 + +0.7
1,359,217 + +0.7
1,359,224 + +0.5
1,359,330 - -2.3
1,359,410 + +2.0
1,359,465 - +1.7
1,359,510 + +1.3
1,359,510 + -0.3
1,359,557 + +1.7

Or see this region's nucleotide sequence