Experiment: BHIS with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0683 and BT0684 are separated by 187 nucleotides BT0684 and BT0685 are separated by 351 nucleotides
BT0683: BT0683 - alpha-glucosidase (NCBI ptt file), at 847,186 to 849,201
BT0683
BT0684: BT0684 - putative long-chain-fatty-acid--CoA ligase (NCBI ptt file), at 849,389 to 851,053
BT0684
BT0685: BT0685 - putative Na+/H+ exchanger (NCBI ptt file), at 851,405 to 852,877
BT0685
Position (kb) 849
850
851
852 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 848.434 kb on - strand, within BT0683 at 848.666 kb on - strand, within BT0683 at 848.788 kb on - strand, within BT0683 at 848.804 kb on - strand, within BT0683 at 848.842 kb on + strand, within BT0683 at 848.942 kb on + strand, within BT0683 at 848.947 kb on - strand, within BT0683 at 849.042 kb on + strand at 849.056 kb on - strand at 849.100 kb on + strand at 849.199 kb on + strand at 849.202 kb on + strand at 849.214 kb on - strand at 849.216 kb on + strand at 849.220 kb on + strand at 849.220 kb on + strand at 849.231 kb on - strand at 849.303 kb on + strand at 849.303 kb on + strand at 849.409 kb on - strand at 849.643 kb on - strand, within BT0684 at 849.650 kb on - strand, within BT0684 at 849.667 kb on - strand, within BT0684 at 849.742 kb on - strand, within BT0684 at 849.845 kb on - strand, within BT0684 at 849.995 kb on - strand, within BT0684 at 849.995 kb on - strand, within BT0684 at 850.146 kb on - strand, within BT0684 at 850.167 kb on - strand, within BT0684 at 850.201 kb on + strand, within BT0684 at 850.505 kb on - strand, within BT0684 at 850.505 kb on - strand, within BT0684 at 850.506 kb on + strand, within BT0684 at 850.762 kb on + strand, within BT0684 at 850.832 kb on + strand, within BT0684 at 851.174 kb on + strand at 851.310 kb on + strand at 851.373 kb on - strand at 851.375 kb on + strand at 851.386 kb on + strand at 851.398 kb on - strand at 851.605 kb on + strand, within BT0685 at 851.687 kb on + strand, within BT0685 at 851.688 kb on - strand, within BT0685 at 851.754 kb on - strand, within BT0685 at 851.768 kb on - strand, within BT0685 at 851.771 kb on - strand, within BT0685 at 851.948 kb on + strand, within BT0685 at 851.955 kb on - strand, within BT0685
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM remove 848,434 - BT0683 0.62 -0.2 848,666 - BT0683 0.73 +0.8 848,788 - BT0683 0.79 -1.2 848,804 - BT0683 0.80 -1.3 848,842 + BT0683 0.82 -1.2 848,942 + BT0683 0.87 -3.7 848,947 - BT0683 0.87 -0.8 849,042 + +1.4 849,056 - +1.9 849,100 + -0.2 849,199 + -0.8 849,202 + -0.2 849,214 - -1.8 849,216 + -0.2 849,220 + +0.5 849,220 + -0.8 849,231 - -0.2 849,303 + +0.6 849,303 + -1.8 849,409 - -1.1 849,643 - BT0684 0.15 -2.5 849,650 - BT0684 0.16 +1.1 849,667 - BT0684 0.17 +3.1 849,742 - BT0684 0.21 -1.2 849,845 - BT0684 0.27 +0.6 849,995 - BT0684 0.36 +0.8 849,995 - BT0684 0.36 -1.9 850,146 - BT0684 0.45 +0.6 850,167 - BT0684 0.47 +0.2 850,201 + BT0684 0.49 -2.5 850,505 - BT0684 0.67 -1.8 850,505 - BT0684 0.67 -0.5 850,506 + BT0684 0.67 -1.4 850,762 + BT0684 0.82 +0.8 850,832 + BT0684 0.87 +0.8 851,174 + -0.8 851,310 + +2.1 851,373 - -0.2 851,375 + -1.2 851,386 + +1.1 851,398 - -1.8 851,605 + BT0685 0.14 -1.3 851,687 + BT0685 0.19 +1.6 851,688 - BT0685 0.19 +1.0 851,754 - BT0685 0.24 -0.8 851,768 - BT0685 0.25 -0.9 851,771 - BT0685 0.25 +1.8 851,948 + BT0685 0.37 +3.4 851,955 - BT0685 0.37 +0.6
Or see this region's nucleotide sequence