Experiment: BHIS with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0680 and BT0681 are separated by 97 nucleotides BT0681 and BT0682 are separated by 23 nucleotides BT0682 and BT0683 are separated by 56 nucleotides
BT0680: BT0680 - cation efflux system protein czcA (NCBI ptt file), at 841,814 to 844,939
BT0680
BT0681: BT0681 - two-component system sensor histidine kinase (NCBI ptt file), at 845,037 to 846,368
BT0681
BT0682: BT0682 - two-component system response regulator (NCBI ptt file), at 846,392 to 847,129
BT0682
BT0683: BT0683 - alpha-glucosidase (NCBI ptt file), at 847,186 to 849,201
BT0683
Position (kb) 845
846
847 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 844.118 kb on + strand, within BT0680 at 844.157 kb on - strand, within BT0680 at 844.336 kb on - strand, within BT0680 at 844.429 kb on - strand, within BT0680 at 844.431 kb on + strand, within BT0680 at 844.431 kb on + strand, within BT0680 at 844.431 kb on + strand, within BT0680 at 844.460 kb on - strand, within BT0680 at 844.630 kb on + strand at 844.631 kb on - strand at 844.882 kb on - strand at 844.886 kb on + strand at 844.887 kb on - strand at 844.887 kb on - strand at 844.901 kb on + strand at 845.005 kb on + strand at 845.006 kb on - strand at 845.053 kb on - strand at 845.101 kb on + strand at 845.153 kb on - strand at 845.296 kb on + strand, within BT0681 at 845.561 kb on + strand, within BT0681 at 845.562 kb on - strand, within BT0681 at 845.563 kb on + strand, within BT0681 at 845.807 kb on + strand, within BT0681 at 845.823 kb on - strand, within BT0681 at 845.929 kb on + strand, within BT0681 at 846.015 kb on - strand, within BT0681 at 846.117 kb on - strand, within BT0681 at 846.257 kb on - strand at 846.264 kb on - strand at 846.294 kb on + strand at 846.322 kb on - strand at 846.349 kb on - strand at 846.353 kb on + strand at 846.360 kb on + strand at 846.406 kb on + strand at 846.406 kb on + strand at 846.409 kb on - strand at 846.419 kb on + strand at 846.485 kb on + strand, within BT0682 at 846.485 kb on + strand, within BT0682 at 846.486 kb on - strand, within BT0682 at 846.544 kb on + strand, within BT0682 at 846.544 kb on + strand, within BT0682 at 846.545 kb on - strand, within BT0682 at 846.694 kb on - strand, within BT0682 at 846.833 kb on - strand, within BT0682 at 846.907 kb on + strand, within BT0682 at 846.908 kb on - strand, within BT0682 at 846.962 kb on - strand, within BT0682 at 846.962 kb on - strand, within BT0682 at 847.136 kb on - strand at 847.136 kb on - strand at 847.160 kb on - strand at 847.160 kb on - strand at 847.163 kb on - strand at 847.165 kb on - strand at 847.166 kb on + strand at 847.167 kb on - strand at 847.182 kb on + strand at 847.202 kb on + strand at 847.203 kb on - strand at 847.234 kb on + strand at 847.234 kb on + strand at 847.282 kb on - strand at 847.326 kb on + strand at 847.326 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 5-Fluorouracil 0.2 mM remove 844,118 + BT0680 0.74 +3.7 844,157 - BT0680 0.75 -0.4 844,336 - BT0680 0.81 +0.8 844,429 - BT0680 0.84 +0.4 844,431 + BT0680 0.84 -0.8 844,431 + BT0680 0.84 +2.1 844,431 + BT0680 0.84 +0.4 844,460 - BT0680 0.85 -1.8 844,630 + -0.2 844,631 - -0.2 844,882 - -3.2 844,886 + +1.8 844,887 - +2.7 844,887 - -1.2 844,901 + -0.1 845,005 + -1.2 845,006 - -0.6 845,053 - -3.5 845,101 + -2.8 845,153 - -0.2 845,296 + BT0681 0.19 -0.2 845,561 + BT0681 0.39 +1.8 845,562 - BT0681 0.39 +2.4 845,563 + BT0681 0.39 -0.5 845,807 + BT0681 0.58 +2.1 845,823 - BT0681 0.59 +3.2 845,929 + BT0681 0.67 +2.6 846,015 - BT0681 0.73 -0.2 846,117 - BT0681 0.81 -0.5 846,257 - +0.4 846,264 - +1.8 846,294 + -3.5 846,322 - +3.1 846,349 - +2.1 846,353 + -0.4 846,360 + -2.5 846,406 + +0.4 846,406 + -0.4 846,409 - +2.1 846,419 + -1.2 846,485 + BT0682 0.13 +0.3 846,485 + BT0682 0.13 -2.9 846,486 - BT0682 0.13 -0.2 846,544 + BT0682 0.21 +2.6 846,544 + BT0682 0.21 -1.5 846,545 - BT0682 0.21 -1.8 846,694 - BT0682 0.41 -1.8 846,833 - BT0682 0.60 -2.7 846,907 + BT0682 0.70 -2.8 846,908 - BT0682 0.70 -3.0 846,962 - BT0682 0.77 -1.3 846,962 - BT0682 0.77 +0.8 847,136 - -3.0 847,136 - -2.8 847,160 - +0.8 847,160 - -0.2 847,163 - -4.7 847,165 - -0.5 847,166 + -2.2 847,167 - +0.2 847,182 + -0.2 847,202 + +1.4 847,203 - -2.6 847,234 + -3.0 847,234 + +0.3 847,282 - +2.6 847,326 + -0.6 847,326 + -0.2
Or see this region's nucleotide sequence