Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02247 and CCNA_02248 are separated by 1 nucleotides CCNA_02248 and CCNA_02249 are separated by 74 nucleotides CCNA_02249 and CCNA_02250 are separated by 207 nucleotides CCNA_02250 and CCNA_02251 are separated by 60 nucleotides CCNA_02251 and CCNA_02252 are separated by 21 nucleotides
CCNA_02247: CCNA_02247 - pantoate-beta-alanine ligase, at 2,396,214 to 2,397,071
_02247
CCNA_02248: CCNA_02248 - hypothetical protein, at 2,397,073 to 2,397,162
_02248
CCNA_02249: CCNA_02249 - conserved hypothetical cytosolic protein, at 2,397,237 to 2,397,635
_02249
CCNA_02250: CCNA_02250 - methylcrotonyl-CoA carboxylase biotin-containing subunit, at 2,397,843 to 2,399,807
_02250
CCNA_02251: CCNA_02251 - methylglutaconyl-CoA hydratase, at 2,399,868 to 2,400,707
_02251
CCNA_02252: CCNA_02252 - methylcrotonyl-CoA carboxylase carboxyl transferase subunit, at 2,400,729 to 2,402,321
_02252
Position (kb)
2397
2398
2399
2400 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2397.073 kb on - strand at 2397.073 kb on - strand at 2397.106 kb on + strand, within CCNA_02248 at 2397.191 kb on + strand at 2397.306 kb on - strand, within CCNA_02249 at 2397.311 kb on - strand, within CCNA_02249 at 2397.400 kb on - strand, within CCNA_02249 at 2397.476 kb on - strand, within CCNA_02249 at 2397.512 kb on - strand, within CCNA_02249 at 2397.513 kb on - strand, within CCNA_02249 at 2397.543 kb on + strand, within CCNA_02249 at 2397.584 kb on - strand, within CCNA_02249 at 2397.584 kb on - strand, within CCNA_02249 at 2397.614 kb on + strand at 2397.637 kb on - strand at 2397.646 kb on - strand at 2397.646 kb on - strand at 2397.659 kb on + strand at 2397.697 kb on - strand at 2397.763 kb on + strand at 2397.763 kb on + strand at 2397.771 kb on - strand at 2397.776 kb on - strand at 2397.839 kb on + strand at 2397.841 kb on + strand at 2397.865 kb on - strand at 2397.875 kb on - strand at 2398.020 kb on - strand at 2398.154 kb on - strand, within CCNA_02250 at 2398.261 kb on - strand, within CCNA_02250 at 2398.593 kb on - strand, within CCNA_02250 at 2398.880 kb on - strand, within CCNA_02250 at 2399.023 kb on + strand at 2399.031 kb on - strand, within CCNA_02250 at 2399.031 kb on - strand, within CCNA_02250 at 2399.298 kb on - strand, within CCNA_02250 at 2399.340 kb on - strand, within CCNA_02250 at 2399.508 kb on - strand, within CCNA_02250 at 2399.629 kb on - strand at 2399.653 kb on - strand at 2399.797 kb on + strand at 2399.883 kb on - strand at 2399.883 kb on - strand at 2399.883 kb on - strand at 2399.883 kb on - strand at 2400.456 kb on - strand, within CCNA_02251 at 2400.523 kb on - strand, within CCNA_02251 at 2400.672 kb on - strand at 2400.711 kb on - strand at 2400.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,397,073 - +0.7 2,397,073 - +0.1 2,397,106 + CCNA_02248 0.37 +0.4 2,397,191 + +0.4 2,397,306 - CCNA_02249 0.17 +0.4 2,397,311 - CCNA_02249 0.19 +1.9 2,397,400 - CCNA_02249 0.41 -0.2 2,397,476 - CCNA_02249 0.60 +0.9 2,397,512 - CCNA_02249 0.69 +0.8 2,397,513 - CCNA_02249 0.69 +1.2 2,397,543 + CCNA_02249 0.77 +0.3 2,397,584 - CCNA_02249 0.87 +0.0 2,397,584 - CCNA_02249 0.87 +1.1 2,397,614 + +0.2 2,397,637 - -0.3 2,397,646 - +1.4 2,397,646 - +0.1 2,397,659 + -0.4 2,397,697 - +0.5 2,397,763 + -0.2 2,397,763 + +1.7 2,397,771 - +0.3 2,397,776 - +0.3 2,397,839 + +1.1 2,397,841 + -2.3 2,397,865 - +0.6 2,397,875 - +0.4 2,398,020 - -1.1 2,398,154 - CCNA_02250 0.16 -1.2 2,398,261 - CCNA_02250 0.21 -1.5 2,398,593 - CCNA_02250 0.38 -0.1 2,398,880 - CCNA_02250 0.53 +0.2 2,399,023 + -0.6 2,399,031 - CCNA_02250 0.60 -1.4 2,399,031 - CCNA_02250 0.60 -1.0 2,399,298 - CCNA_02250 0.74 -0.1 2,399,340 - CCNA_02250 0.76 +2.2 2,399,508 - CCNA_02250 0.85 -0.5 2,399,629 - +0.0 2,399,653 - +1.1 2,399,797 + -0.2 2,399,883 - +0.6 2,399,883 - +0.2 2,399,883 - +0.5 2,399,883 - +0.7 2,400,456 - CCNA_02251 0.70 -0.5 2,400,523 - CCNA_02251 0.78 -0.5 2,400,672 - -0.5 2,400,711 - +0.4 2,400,711 - +0.7
Or see this region's nucleotide sequence