Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00890

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00885 and BWI76_RS00890 are separated by 364 nucleotidesBWI76_RS00890 and BWI76_RS00895 overlap by 56 nucleotidesBWI76_RS00895 and BWI76_RS00900 overlap by 20 nucleotides BWI76_RS00885: BWI76_RS00885 - tRNA (uridine(54)-C5)-methyltransferase TrmA, at 181,984 to 183,084 _RS00885 BWI76_RS00890: BWI76_RS00890 - vitamin B12/cobalamin outer membrane transporter, at 183,449 to 185,317 _RS00890 BWI76_RS00895: BWI76_RS00895 - glutamate racemase, at 185,262 to 186,116 _RS00895 BWI76_RS00900: BWI76_RS00900 - hypothetical protein, at 186,097 to 186,364 _RS00900 Position (kb) 183 184 185 186Strain fitness (log2 ratio) -2 -1 0 1at 182.575 kb on - strand, within BWI76_RS00885at 182.637 kb on + strand, within BWI76_RS00885at 182.713 kb on + strand, within BWI76_RS00885at 182.772 kb on + strand, within BWI76_RS00885at 182.812 kb on - strand, within BWI76_RS00885at 183.052 kb on + strandat 183.062 kb on + strandat 183.078 kb on - strandat 183.079 kb on + strandat 183.121 kb on - strandat 183.129 kb on + strandat 183.140 kb on + strandat 183.140 kb on + strandat 183.140 kb on + strandat 183.145 kb on + strandat 183.231 kb on + strandat 183.236 kb on + strandat 183.290 kb on - strandat 183.328 kb on + strandat 183.354 kb on - strandat 183.360 kb on - strandat 183.413 kb on - strandat 183.419 kb on - strandat 183.428 kb on + strandat 183.527 kb on - strandat 183.535 kb on + strandat 183.536 kb on - strandat 183.567 kb on + strandat 183.613 kb on - strandat 183.753 kb on - strand, within BWI76_RS00890at 183.758 kb on + strand, within BWI76_RS00890at 183.786 kb on + strand, within BWI76_RS00890at 183.830 kb on + strand, within BWI76_RS00890at 183.872 kb on + strand, within BWI76_RS00890at 183.880 kb on - strand, within BWI76_RS00890at 183.894 kb on - strand, within BWI76_RS00890at 183.942 kb on + strand, within BWI76_RS00890at 183.942 kb on + strand, within BWI76_RS00890at 183.942 kb on + strand, within BWI76_RS00890at 183.945 kb on - strand, within BWI76_RS00890at 183.951 kb on - strand, within BWI76_RS00890at 184.005 kb on - strand, within BWI76_RS00890at 184.017 kb on + strand, within BWI76_RS00890at 184.017 kb on + strand, within BWI76_RS00890at 184.066 kb on + strand, within BWI76_RS00890at 184.121 kb on - strand, within BWI76_RS00890at 184.170 kb on - strand, within BWI76_RS00890at 184.177 kb on - strand, within BWI76_RS00890at 184.189 kb on - strand, within BWI76_RS00890at 184.282 kb on + strand, within BWI76_RS00890at 184.290 kb on + strand, within BWI76_RS00890at 184.297 kb on + strand, within BWI76_RS00890at 184.334 kb on - strand, within BWI76_RS00890at 184.390 kb on + strand, within BWI76_RS00890at 184.445 kb on + strand, within BWI76_RS00890at 184.448 kb on - strand, within BWI76_RS00890at 184.448 kb on - strand, within BWI76_RS00890at 184.625 kb on + strand, within BWI76_RS00890at 184.680 kb on + strand, within BWI76_RS00890at 184.708 kb on - strand, within BWI76_RS00890at 184.779 kb on - strand, within BWI76_RS00890at 184.855 kb on + strand, within BWI76_RS00890at 184.855 kb on + strand, within BWI76_RS00890at 184.860 kb on + strand, within BWI76_RS00890at 184.938 kb on - strand, within BWI76_RS00890at 184.990 kb on + strand, within BWI76_RS00890at 185.061 kb on - strand, within BWI76_RS00890at 185.095 kb on + strand, within BWI76_RS00890at 185.156 kb on - strandat 185.169 kb on + strandat 185.258 kb on + strandat 186.174 kb on + strand, within BWI76_RS00900

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
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182,575 - BWI76_RS00885 0.54 +0.6
182,637 + BWI76_RS00885 0.59 -1.1
182,713 + BWI76_RS00885 0.66 -0.1
182,772 + BWI76_RS00885 0.72 -0.8
182,812 - BWI76_RS00885 0.75 -0.3
183,052 + +1.3
183,062 + +0.2
183,078 - -0.5
183,079 + -0.7
183,121 - -1.2
183,129 + +1.0
183,140 + -0.3
183,140 + +0.5
183,140 + -2.3
183,145 + +0.0
183,231 + -0.5
183,236 + +0.1
183,290 - +0.2
183,328 + -0.4
183,354 - -1.4
183,360 - -0.8
183,413 - -0.7
183,419 - -1.5
183,428 + -1.7
183,527 - -2.1
183,535 + -1.0
183,536 - -0.6
183,567 + -1.1
183,613 - +0.0
183,753 - BWI76_RS00890 0.16 -0.5
183,758 + BWI76_RS00890 0.17 -1.4
183,786 + BWI76_RS00890 0.18 -0.1
183,830 + BWI76_RS00890 0.20 -0.9
183,872 + BWI76_RS00890 0.23 -0.2
183,880 - BWI76_RS00890 0.23 -1.5
183,894 - BWI76_RS00890 0.24 -0.9
183,942 + BWI76_RS00890 0.26 -0.8
183,942 + BWI76_RS00890 0.26 -1.7
183,942 + BWI76_RS00890 0.26 +0.5
183,945 - BWI76_RS00890 0.27 -0.7
183,951 - BWI76_RS00890 0.27 -0.7
184,005 - BWI76_RS00890 0.30 +1.6
184,017 + BWI76_RS00890 0.30 -0.9
184,017 + BWI76_RS00890 0.30 -1.9
184,066 + BWI76_RS00890 0.33 -1.4
184,121 - BWI76_RS00890 0.36 +0.4
184,170 - BWI76_RS00890 0.39 -1.3
184,177 - BWI76_RS00890 0.39 +0.2
184,189 - BWI76_RS00890 0.40 +0.0
184,282 + BWI76_RS00890 0.45 +0.1
184,290 + BWI76_RS00890 0.45 -0.8
184,297 + BWI76_RS00890 0.45 -1.0
184,334 - BWI76_RS00890 0.47 -0.8
184,390 + BWI76_RS00890 0.50 -0.4
184,445 + BWI76_RS00890 0.53 -0.8
184,448 - BWI76_RS00890 0.53 -0.9
184,448 - BWI76_RS00890 0.53 -0.6
184,625 + BWI76_RS00890 0.63 +0.9
184,680 + BWI76_RS00890 0.66 -1.4
184,708 - BWI76_RS00890 0.67 -1.2
184,779 - BWI76_RS00890 0.71 -0.6
184,855 + BWI76_RS00890 0.75 -2.2
184,855 + BWI76_RS00890 0.75 -1.3
184,860 + BWI76_RS00890 0.75 -0.3
184,938 - BWI76_RS00890 0.80 -0.1
184,990 + BWI76_RS00890 0.82 -0.9
185,061 - BWI76_RS00890 0.86 -0.8
185,095 + BWI76_RS00890 0.88 -0.8
185,156 - -0.4
185,169 + -1.7
185,258 + -0.5
186,174 + BWI76_RS00900 0.29 -0.2

Or see this region's nucleotide sequence