Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4726 and Psyr_4727 are separated by 70 nucleotides Psyr_4727 and Psyr_4728 are separated by 69 nucleotides Psyr_4728 and Psyr_4729 overlap by 4 nucleotides Psyr_4729 and Psyr_4730 are separated by 121 nucleotides
Psyr_4726: Psyr_4726 - hypothetical protein, at 5,604,413 to 5,605,573
_4726
Psyr_4727: Psyr_4727 - glutathione S-transferase, putative, at 5,605,644 to 5,606,273
_4727
Psyr_4728: Psyr_4728 - conserved hypothetical protein, at 5,606,343 to 5,606,525
_4728
Psyr_4729: Psyr_4729 - PepSY-associated TM helix, at 5,606,522 to 5,607,655
_4729
Psyr_4730: Psyr_4730 - FecR protein, at 5,607,777 to 5,608,760
_4730
Position (kb)
5606
5607
5608 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 5605.717 kb on + strand, within Psyr_4727 at 5605.837 kb on + strand, within Psyr_4727 at 5605.837 kb on + strand, within Psyr_4727 at 5605.837 kb on + strand, within Psyr_4727 at 5605.838 kb on - strand, within Psyr_4727 at 5605.838 kb on - strand, within Psyr_4727 at 5605.838 kb on - strand, within Psyr_4727 at 5606.041 kb on + strand, within Psyr_4727 at 5606.042 kb on - strand, within Psyr_4727 at 5606.158 kb on - strand, within Psyr_4727 at 5606.248 kb on + strand at 5606.248 kb on + strand at 5606.248 kb on + strand at 5606.248 kb on + strand at 5606.248 kb on + strand at 5606.249 kb on - strand at 5606.249 kb on - strand at 5606.249 kb on - strand at 5606.277 kb on + strand at 5606.301 kb on + strand at 5606.301 kb on + strand at 5606.301 kb on + strand at 5606.301 kb on + strand at 5606.301 kb on + strand at 5606.302 kb on - strand at 5606.302 kb on - strand at 5606.302 kb on - strand at 5606.302 kb on - strand at 5606.306 kb on + strand at 5606.306 kb on + strand at 5606.307 kb on - strand at 5606.372 kb on + strand, within Psyr_4728 at 5606.589 kb on + strand at 5606.589 kb on + strand at 5606.606 kb on + strand at 5606.709 kb on + strand, within Psyr_4729 at 5606.710 kb on - strand, within Psyr_4729 at 5606.794 kb on + strand, within Psyr_4729 at 5606.794 kb on + strand, within Psyr_4729 at 5606.795 kb on - strand, within Psyr_4729 at 5606.795 kb on - strand, within Psyr_4729 at 5606.795 kb on - strand, within Psyr_4729 at 5606.949 kb on + strand, within Psyr_4729 at 5606.950 kb on - strand, within Psyr_4729 at 5607.062 kb on + strand, within Psyr_4729 at 5607.063 kb on - strand, within Psyr_4729 at 5607.097 kb on + strand, within Psyr_4729 at 5607.109 kb on + strand, within Psyr_4729 at 5607.109 kb on + strand, within Psyr_4729 at 5607.109 kb on + strand, within Psyr_4729 at 5607.110 kb on - strand, within Psyr_4729 at 5607.110 kb on - strand, within Psyr_4729 at 5607.156 kb on + strand, within Psyr_4729 at 5607.156 kb on + strand, within Psyr_4729 at 5607.157 kb on - strand, within Psyr_4729 at 5607.237 kb on + strand, within Psyr_4729 at 5607.237 kb on + strand, within Psyr_4729 at 5607.237 kb on + strand, within Psyr_4729 at 5607.237 kb on + strand, within Psyr_4729 at 5607.237 kb on + strand, within Psyr_4729 at 5607.237 kb on + strand, within Psyr_4729 at 5607.238 kb on - strand, within Psyr_4729 at 5607.238 kb on - strand, within Psyr_4729 at 5607.238 kb on - strand, within Psyr_4729 at 5607.238 kb on - strand, within Psyr_4729 at 5607.238 kb on - strand, within Psyr_4729 at 5607.245 kb on + strand, within Psyr_4729 at 5607.245 kb on + strand, within Psyr_4729 at 5607.245 kb on + strand, within Psyr_4729 at 5607.245 kb on + strand, within Psyr_4729 at 5607.245 kb on + strand, within Psyr_4729 at 5607.246 kb on - strand, within Psyr_4729 at 5607.246 kb on - strand, within Psyr_4729 at 5607.246 kb on - strand, within Psyr_4729 at 5607.246 kb on - strand, within Psyr_4729 at 5607.418 kb on - strand, within Psyr_4729 at 5607.508 kb on - strand, within Psyr_4729 at 5607.508 kb on - strand, within Psyr_4729 at 5607.616 kb on + strand at 5607.616 kb on + strand at 5607.617 kb on - strand at 5607.617 kb on - strand at 5607.617 kb on - strand at 5607.660 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.710 kb on + strand at 5607.711 kb on - strand at 5607.711 kb on - strand at 5607.711 kb on - strand at 5607.711 kb on - strand at 5607.711 kb on - strand at 5607.711 kb on - strand at 5607.711 kb on - strand at 5607.716 kb on + strand at 5607.716 kb on + strand at 5607.716 kb on + strand at 5607.717 kb on - strand at 5607.771 kb on + strand at 5607.771 kb on + strand at 5607.772 kb on - strand at 5607.852 kb on + strand at 5607.852 kb on + strand at 5607.853 kb on - strand at 5607.853 kb on - strand at 5607.853 kb on - strand at 5607.853 kb on - strand at 5607.853 kb on - strand at 5607.853 kb on - strand at 5608.297 kb on - strand, within Psyr_4730 at 5608.297 kb on - strand, within Psyr_4730 at 5608.297 kb on - strand, within Psyr_4730
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 5,605,717 + Psyr_4727 0.12 -1.7 5,605,837 + Psyr_4727 0.31 -3.6 5,605,837 + Psyr_4727 0.31 +1.1 5,605,837 + Psyr_4727 0.31 -0.5 5,605,838 - Psyr_4727 0.31 -3.0 5,605,838 - Psyr_4727 0.31 +0.1 5,605,838 - Psyr_4727 0.31 +0.6 5,606,041 + Psyr_4727 0.63 -1.0 5,606,042 - Psyr_4727 0.63 +2.7 5,606,158 - Psyr_4727 0.82 +2.2 5,606,248 + +3.9 5,606,248 + -0.8 5,606,248 + +2.6 5,606,248 + -1.5 5,606,248 + -1.2 5,606,249 - +1.1 5,606,249 - +1.4 5,606,249 - +0.1 5,606,277 + -0.3 5,606,301 + -2.0 5,606,301 + +2.3 5,606,301 + +0.5 5,606,301 + +0.6 5,606,301 + +0.8 5,606,302 - +0.9 5,606,302 - -0.3 5,606,302 - -1.7 5,606,302 - -1.9 5,606,306 + +1.5 5,606,306 + +0.9 5,606,307 - -0.2 5,606,372 + Psyr_4728 0.16 +1.3 5,606,589 + +0.2 5,606,589 + +2.6 5,606,606 + +0.6 5,606,709 + Psyr_4729 0.16 -1.7 5,606,710 - Psyr_4729 0.17 -2.8 5,606,794 + Psyr_4729 0.24 +0.3 5,606,794 + Psyr_4729 0.24 -0.2 5,606,795 - Psyr_4729 0.24 -1.5 5,606,795 - Psyr_4729 0.24 -0.3 5,606,795 - Psyr_4729 0.24 -1.1 5,606,949 + Psyr_4729 0.38 +1.6 5,606,950 - Psyr_4729 0.38 -0.3 5,607,062 + Psyr_4729 0.48 +0.9 5,607,063 - Psyr_4729 0.48 -0.3 5,607,097 + Psyr_4729 0.51 +0.2 5,607,109 + Psyr_4729 0.52 -1.3 5,607,109 + Psyr_4729 0.52 -0.3 5,607,109 + Psyr_4729 0.52 -1.7 5,607,110 - Psyr_4729 0.52 -2.4 5,607,110 - Psyr_4729 0.52 +1.4 5,607,156 + Psyr_4729 0.56 -0.8 5,607,156 + Psyr_4729 0.56 +0.1 5,607,157 - Psyr_4729 0.56 +0.4 5,607,237 + Psyr_4729 0.63 +1.1 5,607,237 + Psyr_4729 0.63 +0.8 5,607,237 + Psyr_4729 0.63 -2.0 5,607,237 + Psyr_4729 0.63 -3.8 5,607,237 + Psyr_4729 0.63 +0.8 5,607,237 + Psyr_4729 0.63 -2.4 5,607,238 - Psyr_4729 0.63 +0.8 5,607,238 - Psyr_4729 0.63 +0.2 5,607,238 - Psyr_4729 0.63 +0.4 5,607,238 - Psyr_4729 0.63 -1.9 5,607,238 - Psyr_4729 0.63 -3.0 5,607,245 + Psyr_4729 0.64 -1.5 5,607,245 + Psyr_4729 0.64 -0.4 5,607,245 + Psyr_4729 0.64 +1.8 5,607,245 + Psyr_4729 0.64 -3.1 5,607,245 + Psyr_4729 0.64 -0.6 5,607,246 - Psyr_4729 0.64 +1.4 5,607,246 - Psyr_4729 0.64 +0.3 5,607,246 - Psyr_4729 0.64 -3.9 5,607,246 - Psyr_4729 0.64 +1.5 5,607,418 - Psyr_4729 0.79 -1.9 5,607,508 - Psyr_4729 0.87 -1.9 5,607,508 - Psyr_4729 0.87 -3.3 5,607,616 + -0.2 5,607,616 + -0.5 5,607,617 - +1.1 5,607,617 - -0.8 5,607,617 - -2.5 5,607,660 + +2.1 5,607,710 + +1.9 5,607,710 + -0.4 5,607,710 + -3.0 5,607,710 + -2.4 5,607,710 + -0.0 5,607,710 + +0.0 5,607,710 + +0.3 5,607,710 + +3.7 5,607,710 + -0.7 5,607,710 + +1.2 5,607,710 + -2.9 5,607,710 + +0.7 5,607,711 - +1.1 5,607,711 - -0.1 5,607,711 - -0.7 5,607,711 - +1.5 5,607,711 - -3.6 5,607,711 - -1.1 5,607,711 - +1.7 5,607,716 + +2.3 5,607,716 + +1.0 5,607,716 + -0.7 5,607,717 - +1.3 5,607,771 + +0.1 5,607,771 + +1.0 5,607,772 - -3.6 5,607,852 + -0.7 5,607,852 + +0.2 5,607,853 - -0.8 5,607,853 - -3.5 5,607,853 - -1.7 5,607,853 - -0.5 5,607,853 - -1.3 5,607,853 - -0.1 5,608,297 - Psyr_4730 0.53 +1.5 5,608,297 - Psyr_4730 0.53 +1.3 5,608,297 - Psyr_4730 0.53 +1.2
Or see this region's nucleotide sequence