Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3647 and Psyr_3648 are separated by 304 nucleotides Psyr_3648 and Psyr_3649 are separated by 99 nucleotides
Psyr_3647: Psyr_3647 - DNA gyrase subunit A, at 4,330,537 to 4,333,314
_3647
Psyr_3648: Psyr_3648 - methylthioribose-1-phosphate isomerase, at 4,333,619 to 4,334,716
_3648
Psyr_3649: Psyr_3649 - Amidohydrolase, at 4,334,816 to 4,336,147
_3649
Position (kb)
4333
4334
4335 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4333.425 kb on - strand at 4333.425 kb on - strand at 4333.438 kb on - strand at 4333.438 kb on - strand at 4333.546 kb on + strand at 4333.567 kb on - strand at 4333.633 kb on + strand at 4333.914 kb on + strand, within Psyr_3648 at 4333.914 kb on + strand, within Psyr_3648 at 4333.914 kb on + strand, within Psyr_3648 at 4333.914 kb on + strand, within Psyr_3648 at 4333.915 kb on - strand, within Psyr_3648 at 4333.993 kb on + strand, within Psyr_3648 at 4334.109 kb on + strand, within Psyr_3648 at 4334.110 kb on - strand, within Psyr_3648 at 4334.110 kb on - strand, within Psyr_3648 at 4334.110 kb on - strand, within Psyr_3648 at 4334.502 kb on + strand, within Psyr_3648 at 4334.503 kb on - strand, within Psyr_3648 at 4334.581 kb on + strand, within Psyr_3648 at 4334.581 kb on + strand, within Psyr_3648 at 4334.581 kb on + strand, within Psyr_3648 at 4334.581 kb on + strand, within Psyr_3648 at 4334.582 kb on - strand, within Psyr_3648 at 4334.582 kb on - strand, within Psyr_3648 at 4334.631 kb on + strand at 4334.631 kb on + strand at 4334.632 kb on - strand at 4334.747 kb on + strand at 4334.747 kb on + strand at 4334.776 kb on - strand at 4334.777 kb on + strand at 4334.777 kb on + strand at 4334.777 kb on + strand at 4334.778 kb on - strand at 4334.778 kb on - strand at 4334.778 kb on - strand at 4334.778 kb on - strand at 4334.778 kb on - strand at 4334.778 kb on - strand at 4334.793 kb on + strand at 4334.793 kb on + strand at 4334.793 kb on + strand at 4334.793 kb on + strand at 4334.794 kb on - strand at 4334.796 kb on + strand at 4334.797 kb on - strand at 4334.929 kb on + strand at 4334.936 kb on + strand at 4334.936 kb on + strand at 4334.936 kb on + strand at 4334.936 kb on + strand at 4334.936 kb on + strand at 4334.937 kb on - strand at 4334.937 kb on - strand at 4334.937 kb on - strand at 4334.937 kb on - strand at 4334.938 kb on + strand at 4334.939 kb on - strand at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.212 kb on + strand, within Psyr_3649 at 4335.213 kb on - strand, within Psyr_3649 at 4335.213 kb on - strand, within Psyr_3649 at 4335.213 kb on - strand, within Psyr_3649 at 4335.213 kb on - strand, within Psyr_3649 at 4335.213 kb on - strand, within Psyr_3649 at 4335.213 kb on - strand, within Psyr_3649 at 4335.213 kb on - strand, within Psyr_3649 at 4335.404 kb on + strand, within Psyr_3649 at 4335.404 kb on + strand, within Psyr_3649 at 4335.405 kb on - strand, within Psyr_3649
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 4,333,425 - +3.1 4,333,425 - +0.0 4,333,438 - -3.3 4,333,438 - +0.5 4,333,546 + -3.9 4,333,567 - -0.7 4,333,633 + +0.8 4,333,914 + Psyr_3648 0.27 -0.9 4,333,914 + Psyr_3648 0.27 -2.7 4,333,914 + Psyr_3648 0.27 -1.2 4,333,914 + Psyr_3648 0.27 -0.6 4,333,915 - Psyr_3648 0.27 -0.3 4,333,993 + Psyr_3648 0.34 -2.7 4,334,109 + Psyr_3648 0.45 +0.1 4,334,110 - Psyr_3648 0.45 +3.7 4,334,110 - Psyr_3648 0.45 -2.8 4,334,110 - Psyr_3648 0.45 +1.7 4,334,502 + Psyr_3648 0.80 -2.8 4,334,503 - Psyr_3648 0.81 -1.1 4,334,581 + Psyr_3648 0.88 +1.7 4,334,581 + Psyr_3648 0.88 -1.7 4,334,581 + Psyr_3648 0.88 -0.5 4,334,581 + Psyr_3648 0.88 -2.0 4,334,582 - Psyr_3648 0.88 -1.9 4,334,582 - Psyr_3648 0.88 -1.6 4,334,631 + -0.8 4,334,631 + +0.5 4,334,632 - -0.4 4,334,747 + +0.3 4,334,747 + +0.8 4,334,776 - +1.6 4,334,777 + +0.8 4,334,777 + -1.1 4,334,777 + -3.0 4,334,778 - -0.4 4,334,778 - -1.1 4,334,778 - -2.8 4,334,778 - -0.2 4,334,778 - -1.2 4,334,778 - +0.9 4,334,793 + -2.2 4,334,793 + -1.7 4,334,793 + +0.5 4,334,793 + -0.2 4,334,794 - -1.8 4,334,796 + -1.1 4,334,797 - +0.1 4,334,929 + +1.0 4,334,936 + -0.8 4,334,936 + -2.7 4,334,936 + -1.8 4,334,936 + -3.1 4,334,936 + -3.0 4,334,937 - -0.5 4,334,937 - -0.7 4,334,937 - +0.9 4,334,937 - -1.7 4,334,938 + -1.4 4,334,939 - -1.4 4,335,212 + Psyr_3649 0.30 -3.4 4,335,212 + Psyr_3649 0.30 -0.1 4,335,212 + Psyr_3649 0.30 -1.6 4,335,212 + Psyr_3649 0.30 -1.8 4,335,212 + Psyr_3649 0.30 -1.8 4,335,212 + Psyr_3649 0.30 -1.5 4,335,212 + Psyr_3649 0.30 -1.5 4,335,212 + Psyr_3649 0.30 -0.3 4,335,212 + Psyr_3649 0.30 -4.1 4,335,212 + Psyr_3649 0.30 -0.6 4,335,212 + Psyr_3649 0.30 -2.7 4,335,212 + Psyr_3649 0.30 -0.3 4,335,212 + Psyr_3649 0.30 +0.0 4,335,212 + Psyr_3649 0.30 -0.5 4,335,212 + Psyr_3649 0.30 -2.7 4,335,212 + Psyr_3649 0.30 -2.2 4,335,212 + Psyr_3649 0.30 -2.1 4,335,212 + Psyr_3649 0.30 +0.0 4,335,213 - Psyr_3649 0.30 -0.9 4,335,213 - Psyr_3649 0.30 -0.7 4,335,213 - Psyr_3649 0.30 -0.9 4,335,213 - Psyr_3649 0.30 +0.1 4,335,213 - Psyr_3649 0.30 -0.8 4,335,213 - Psyr_3649 0.30 -1.5 4,335,213 - Psyr_3649 0.30 -1.2 4,335,404 + Psyr_3649 0.44 -1.3 4,335,404 + Psyr_3649 0.44 -1.0 4,335,405 - Psyr_3649 0.44 -0.4
Or see this region's nucleotide sequence