Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3346 and Psyr_3347 are separated by 184 nucleotides Psyr_3347 and Psyr_3348 are separated by 132 nucleotides Psyr_3348 and Psyr_3349 are separated by 141 nucleotides Psyr_3349 and Psyr_3350 are separated by 28 nucleotides
Psyr_3346: Psyr_3346 - conserved hypothetical protein, at 4,002,523 to 4,003,044
_3346
Psyr_3347: Psyr_3347 - transcriptional regulator, TetR family, at 4,003,229 to 4,003,846
_3347
Psyr_3348: Psyr_3348 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 4,003,979 to 4,005,607
_3348
Psyr_3349: Psyr_3349 - conserved hypothetical protein, at 4,005,749 to 4,006,177
_3349
Psyr_3350: Psyr_3350 - Allergen V5/Tpx-1 related protein, at 4,006,206 to 4,007,087
_3350
Position (kb)
4003
4004
4005
4006 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4003.044 kb on + strand at 4003.044 kb on + strand at 4003.044 kb on + strand at 4003.045 kb on - strand at 4003.045 kb on - strand at 4003.045 kb on - strand at 4003.111 kb on + strand at 4003.111 kb on + strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.112 kb on - strand at 4003.115 kb on + strand at 4003.116 kb on - strand at 4003.116 kb on - strand at 4003.116 kb on - strand at 4003.116 kb on - strand at 4003.133 kb on - strand at 4003.225 kb on - strand at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.361 kb on + strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.362 kb on - strand, within Psyr_3347 at 4003.363 kb on + strand, within Psyr_3347 at 4003.363 kb on + strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.364 kb on - strand, within Psyr_3347 at 4003.367 kb on + strand, within Psyr_3347 at 4003.367 kb on + strand, within Psyr_3347 at 4003.367 kb on + strand, within Psyr_3347 at 4003.367 kb on + strand, within Psyr_3347 at 4003.368 kb on - strand, within Psyr_3347 at 4003.368 kb on - strand, within Psyr_3347 at 4003.368 kb on - strand, within Psyr_3347 at 4003.368 kb on - strand, within Psyr_3347 at 4003.368 kb on - strand, within Psyr_3347 at 4003.368 kb on - strand, within Psyr_3347 at 4003.368 kb on - strand, within Psyr_3347 at 4003.368 kb on - strand, within Psyr_3347 at 4003.525 kb on + strand, within Psyr_3347 at 4003.525 kb on + strand, within Psyr_3347 at 4003.553 kb on + strand, within Psyr_3347 at 4003.605 kb on - strand, within Psyr_3347 at 4003.605 kb on - strand, within Psyr_3347 at 4003.639 kb on + strand, within Psyr_3347 at 4003.640 kb on - strand, within Psyr_3347 at 4003.640 kb on - strand, within Psyr_3347 at 4003.640 kb on - strand, within Psyr_3347 at 4003.886 kb on - strand at 4003.886 kb on - strand at 4003.959 kb on - strand at 4003.974 kb on + strand at 4003.974 kb on + strand at 4003.974 kb on + strand at 4003.975 kb on - strand at 4003.975 kb on - strand at 4003.975 kb on - strand at 4003.975 kb on - strand at 4004.274 kb on - strand, within Psyr_3348 at 4004.575 kb on - strand, within Psyr_3348 at 4004.609 kb on + strand, within Psyr_3348 at 4004.609 kb on + strand, within Psyr_3348 at 4004.610 kb on - strand, within Psyr_3348 at 4004.610 kb on - strand, within Psyr_3348 at 4004.610 kb on - strand, within Psyr_3348 at 4004.610 kb on - strand, within Psyr_3348 at 4004.610 kb on - strand, within Psyr_3348 at 4004.622 kb on + strand, within Psyr_3348 at 4004.623 kb on - strand, within Psyr_3348 at 4004.623 kb on - strand, within Psyr_3348 at 4004.651 kb on - strand, within Psyr_3348 at 4004.917 kb on - strand, within Psyr_3348 at 4005.063 kb on + strand, within Psyr_3348 at 4005.063 kb on + strand, within Psyr_3348 at 4005.064 kb on - strand, within Psyr_3348 at 4005.064 kb on - strand, within Psyr_3348 at 4005.064 kb on - strand, within Psyr_3348 at 4005.064 kb on - strand, within Psyr_3348 at 4005.064 kb on - strand, within Psyr_3348 at 4005.064 kb on - strand, within Psyr_3348 at 4005.064 kb on - strand, within Psyr_3348 at 4005.097 kb on + strand, within Psyr_3348 at 4005.097 kb on + strand, within Psyr_3348 at 4005.606 kb on - strand at 4005.727 kb on - strand at 4005.727 kb on - strand at 4005.745 kb on - strand at 4005.917 kb on + strand, within Psyr_3349 at 4005.917 kb on + strand, within Psyr_3349 at 4005.917 kb on + strand, within Psyr_3349 at 4005.917 kb on + strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.918 kb on - strand, within Psyr_3349 at 4005.937 kb on + strand, within Psyr_3349 at 4005.937 kb on + strand, within Psyr_3349 at 4005.938 kb on - strand, within Psyr_3349 at 4005.938 kb on - strand, within Psyr_3349 at 4005.938 kb on - strand, within Psyr_3349 at 4005.938 kb on - strand, within Psyr_3349 at 4005.938 kb on - strand, within Psyr_3349 at 4005.938 kb on - strand, within Psyr_3349 at 4006.237 kb on + strand at 4006.405 kb on + strand, within Psyr_3350
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 4,003,044 + -2.1 4,003,044 + -1.6 4,003,044 + +1.6 4,003,045 - -2.1 4,003,045 - -2.5 4,003,045 - -2.1 4,003,111 + -3.4 4,003,111 + -0.7 4,003,112 - -2.9 4,003,112 - -2.6 4,003,112 - +2.3 4,003,112 - -0.0 4,003,112 - +0.5 4,003,112 - -0.1 4,003,112 - -3.8 4,003,112 - -0.4 4,003,112 - -2.7 4,003,112 - -1.9 4,003,115 + +0.4 4,003,116 - +1.4 4,003,116 - -3.3 4,003,116 - -2.8 4,003,116 - -3.2 4,003,133 - +0.8 4,003,225 - -2.4 4,003,361 + Psyr_3347 0.21 +0.1 4,003,361 + Psyr_3347 0.21 -2.4 4,003,361 + Psyr_3347 0.21 -0.9 4,003,361 + Psyr_3347 0.21 +0.9 4,003,361 + Psyr_3347 0.21 +1.1 4,003,361 + Psyr_3347 0.21 +0.1 4,003,361 + Psyr_3347 0.21 +1.8 4,003,361 + Psyr_3347 0.21 -1.1 4,003,361 + Psyr_3347 0.21 +0.5 4,003,361 + Psyr_3347 0.21 -1.4 4,003,361 + Psyr_3347 0.21 -2.7 4,003,361 + Psyr_3347 0.21 +1.3 4,003,361 + Psyr_3347 0.21 -0.9 4,003,362 - Psyr_3347 0.22 -1.6 4,003,362 - Psyr_3347 0.22 -2.6 4,003,362 - Psyr_3347 0.22 +1.3 4,003,362 - Psyr_3347 0.22 -2.1 4,003,362 - Psyr_3347 0.22 -2.5 4,003,362 - Psyr_3347 0.22 -0.6 4,003,362 - Psyr_3347 0.22 -1.2 4,003,362 - Psyr_3347 0.22 -2.1 4,003,362 - Psyr_3347 0.22 -2.9 4,003,362 - Psyr_3347 0.22 -1.9 4,003,362 - Psyr_3347 0.22 -1.8 4,003,362 - Psyr_3347 0.22 +0.8 4,003,362 - Psyr_3347 0.22 -2.8 4,003,362 - Psyr_3347 0.22 -0.4 4,003,362 - Psyr_3347 0.22 -0.8 4,003,363 + Psyr_3347 0.22 -2.9 4,003,363 + Psyr_3347 0.22 -2.4 4,003,364 - Psyr_3347 0.22 +0.1 4,003,364 - Psyr_3347 0.22 -1.4 4,003,364 - Psyr_3347 0.22 +0.8 4,003,364 - Psyr_3347 0.22 +0.1 4,003,364 - Psyr_3347 0.22 -1.3 4,003,364 - Psyr_3347 0.22 -0.1 4,003,364 - Psyr_3347 0.22 -1.2 4,003,364 - Psyr_3347 0.22 -1.9 4,003,364 - Psyr_3347 0.22 -0.1 4,003,364 - Psyr_3347 0.22 -0.2 4,003,364 - Psyr_3347 0.22 -1.3 4,003,367 + Psyr_3347 0.22 +0.3 4,003,367 + Psyr_3347 0.22 -2.2 4,003,367 + Psyr_3347 0.22 -1.9 4,003,367 + Psyr_3347 0.22 -1.2 4,003,368 - Psyr_3347 0.22 -0.8 4,003,368 - Psyr_3347 0.22 -1.2 4,003,368 - Psyr_3347 0.22 -0.7 4,003,368 - Psyr_3347 0.22 -1.7 4,003,368 - Psyr_3347 0.22 -2.0 4,003,368 - Psyr_3347 0.22 -1.0 4,003,368 - Psyr_3347 0.22 +0.1 4,003,368 - Psyr_3347 0.22 +1.1 4,003,525 + Psyr_3347 0.48 -2.0 4,003,525 + Psyr_3347 0.48 +1.3 4,003,553 + Psyr_3347 0.52 -2.4 4,003,605 - Psyr_3347 0.61 -1.6 4,003,605 - Psyr_3347 0.61 -1.9 4,003,639 + Psyr_3347 0.66 -2.0 4,003,640 - Psyr_3347 0.67 -1.5 4,003,640 - Psyr_3347 0.67 -0.5 4,003,640 - Psyr_3347 0.67 +1.2 4,003,886 - +1.6 4,003,886 - +1.0 4,003,959 - -0.9 4,003,974 + +1.1 4,003,974 + +2.5 4,003,974 + -2.3 4,003,975 - -1.0 4,003,975 - +1.2 4,003,975 - +3.9 4,003,975 - -1.0 4,004,274 - Psyr_3348 0.18 +2.8 4,004,575 - Psyr_3348 0.37 +4.4 4,004,609 + Psyr_3348 0.39 +1.5 4,004,609 + Psyr_3348 0.39 +1.5 4,004,610 - Psyr_3348 0.39 +2.2 4,004,610 - Psyr_3348 0.39 -3.0 4,004,610 - Psyr_3348 0.39 +1.5 4,004,610 - Psyr_3348 0.39 -0.0 4,004,610 - Psyr_3348 0.39 -1.0 4,004,622 + Psyr_3348 0.39 -2.4 4,004,623 - Psyr_3348 0.40 -2.7 4,004,623 - Psyr_3348 0.40 -1.5 4,004,651 - Psyr_3348 0.41 +0.6 4,004,917 - Psyr_3348 0.58 +0.4 4,005,063 + Psyr_3348 0.67 -1.9 4,005,063 + Psyr_3348 0.67 +0.5 4,005,064 - Psyr_3348 0.67 +0.6 4,005,064 - Psyr_3348 0.67 -3.1 4,005,064 - Psyr_3348 0.67 -2.9 4,005,064 - Psyr_3348 0.67 -1.3 4,005,064 - Psyr_3348 0.67 +2.6 4,005,064 - Psyr_3348 0.67 -3.1 4,005,064 - Psyr_3348 0.67 +1.6 4,005,097 + Psyr_3348 0.69 +1.1 4,005,097 + Psyr_3348 0.69 -0.1 4,005,606 - +1.9 4,005,727 - +0.7 4,005,727 - +2.7 4,005,745 - +0.6 4,005,917 + Psyr_3349 0.39 +1.4 4,005,917 + Psyr_3349 0.39 +0.8 4,005,917 + Psyr_3349 0.39 +2.9 4,005,917 + Psyr_3349 0.39 -0.5 4,005,918 - Psyr_3349 0.39 -0.5 4,005,918 - Psyr_3349 0.39 -1.7 4,005,918 - Psyr_3349 0.39 -0.3 4,005,918 - Psyr_3349 0.39 -3.5 4,005,918 - Psyr_3349 0.39 -2.2 4,005,918 - Psyr_3349 0.39 -1.3 4,005,918 - Psyr_3349 0.39 -0.2 4,005,918 - Psyr_3349 0.39 -0.4 4,005,918 - Psyr_3349 0.39 +0.8 4,005,918 - Psyr_3349 0.39 +0.6 4,005,918 - Psyr_3349 0.39 -1.9 4,005,937 + Psyr_3349 0.44 -2.7 4,005,937 + Psyr_3349 0.44 +0.8 4,005,938 - Psyr_3349 0.44 -3.1 4,005,938 - Psyr_3349 0.44 -2.1 4,005,938 - Psyr_3349 0.44 -2.0 4,005,938 - Psyr_3349 0.44 -1.6 4,005,938 - Psyr_3349 0.44 -1.9 4,005,938 - Psyr_3349 0.44 -0.1 4,006,237 + -3.1 4,006,405 + Psyr_3350 0.23 +0.4
Or see this region's nucleotide sequence