Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2974 and Psyr_2975 are separated by 124 nucleotides Psyr_2975 and Psyr_2976 are separated by 110 nucleotides Psyr_2976 and Psyr_2977 are separated by 177 nucleotides
Psyr_2974: Psyr_2974 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,566,107 to 3,567,669
_2974
Psyr_2975: Psyr_2975 - Alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen, at 3,567,794 to 3,568,357
_2975
Psyr_2976: Psyr_2976 - Phage integrase:Phage integrase, N-terminal SAM-like protein, at 3,568,468 to 3,569,427
_2976
Psyr_2977: Psyr_2977 - cointegrate resolution protein T, at 3,569,605 to 3,570,615
_2977
Position (kb)
3568
3569
3570 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3567.489 kb on + strand, within Psyr_2974 at 3567.489 kb on + strand, within Psyr_2974 at 3567.490 kb on - strand, within Psyr_2974 at 3567.490 kb on - strand, within Psyr_2974 at 3567.686 kb on + strand at 3567.686 kb on + strand at 3567.695 kb on + strand at 3567.701 kb on + strand at 3567.702 kb on - strand at 3567.753 kb on - strand at 3567.832 kb on + strand at 3567.832 kb on + strand at 3567.833 kb on - strand at 3567.888 kb on + strand, within Psyr_2975 at 3567.889 kb on - strand, within Psyr_2975 at 3568.028 kb on + strand, within Psyr_2975 at 3568.028 kb on + strand, within Psyr_2975 at 3568.029 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.078 kb on - strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.152 kb on + strand, within Psyr_2975 at 3568.153 kb on - strand, within Psyr_2975 at 3568.153 kb on - strand, within Psyr_2975 at 3568.153 kb on - strand, within Psyr_2975 at 3568.153 kb on - strand, within Psyr_2975 at 3568.185 kb on + strand, within Psyr_2975 at 3568.185 kb on + strand, within Psyr_2975 at 3568.185 kb on + strand, within Psyr_2975 at 3568.185 kb on + strand, within Psyr_2975 at 3568.186 kb on - strand, within Psyr_2975 at 3568.220 kb on + strand, within Psyr_2975 at 3568.250 kb on + strand, within Psyr_2975 at 3568.251 kb on - strand, within Psyr_2975 at 3568.335 kb on - strand at 3568.335 kb on - strand at 3568.351 kb on + strand at 3568.361 kb on + strand at 3568.361 kb on + strand at 3568.361 kb on + strand at 3568.362 kb on - strand at 3568.362 kb on - strand at 3568.362 kb on - strand at 3568.362 kb on - strand at 3568.362 kb on - strand at 3568.363 kb on + strand at 3568.364 kb on - strand at 3568.364 kb on - strand at 3568.364 kb on - strand at 3568.410 kb on + strand at 3568.410 kb on + strand at 3568.411 kb on - strand at 3568.411 kb on - strand at 3568.482 kb on + strand at 3568.551 kb on - strand at 3568.551 kb on - strand at 3568.582 kb on + strand, within Psyr_2976 at 3568.582 kb on + strand, within Psyr_2976 at 3568.583 kb on - strand, within Psyr_2976 at 3568.583 kb on - strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.586 kb on + strand, within Psyr_2976 at 3568.587 kb on - strand, within Psyr_2976 at 3568.587 kb on - strand, within Psyr_2976 at 3568.587 kb on - strand, within Psyr_2976 at 3568.587 kb on - strand, within Psyr_2976 at 3568.664 kb on + strand, within Psyr_2976 at 3568.664 kb on + strand, within Psyr_2976 at 3568.665 kb on - strand, within Psyr_2976 at 3568.747 kb on + strand, within Psyr_2976 at 3568.747 kb on + strand, within Psyr_2976 at 3568.748 kb on - strand, within Psyr_2976 at 3568.748 kb on - strand, within Psyr_2976 at 3568.748 kb on - strand, within Psyr_2976 at 3568.752 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.816 kb on + strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.817 kb on - strand, within Psyr_2976 at 3568.818 kb on + strand, within Psyr_2976 at 3568.819 kb on - strand, within Psyr_2976 at 3568.819 kb on - strand, within Psyr_2976 at 3568.820 kb on + strand, within Psyr_2976 at 3568.820 kb on + strand, within Psyr_2976 at 3568.820 kb on + strand, within Psyr_2976 at 3568.820 kb on + strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.821 kb on - strand, within Psyr_2976 at 3568.863 kb on - strand, within Psyr_2976 at 3568.907 kb on + strand, within Psyr_2976 at 3568.907 kb on + strand, within Psyr_2976 at 3568.907 kb on + strand, within Psyr_2976 at 3568.908 kb on - strand, within Psyr_2976 at 3568.908 kb on - strand, within Psyr_2976 at 3568.908 kb on - strand, within Psyr_2976 at 3568.908 kb on - strand, within Psyr_2976 at 3568.948 kb on + strand, within Psyr_2976 at 3568.949 kb on - strand, within Psyr_2976 at 3568.949 kb on - strand, within Psyr_2976 at 3569.111 kb on - strand, within Psyr_2976 at 3569.408 kb on + strand at 3569.499 kb on - strand at 3569.521 kb on - strand at 3569.561 kb on + strand at 3569.595 kb on - strand at 3569.621 kb on + strand at 3569.633 kb on + strand at 3569.634 kb on - strand at 3569.693 kb on + strand at 3569.710 kb on - strand, within Psyr_2977 at 3569.713 kb on - strand, within Psyr_2977 at 3569.740 kb on + strand, within Psyr_2977 at 3569.851 kb on - strand, within Psyr_2977 at 3570.020 kb on + strand, within Psyr_2977 at 3570.020 kb on + strand, within Psyr_2977 at 3570.021 kb on - strand, within Psyr_2977 at 3570.021 kb on - strand, within Psyr_2977 at 3570.021 kb on - strand, within Psyr_2977
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 3,567,489 + Psyr_2974 0.88 -0.2 3,567,489 + Psyr_2974 0.88 -0.8 3,567,490 - Psyr_2974 0.88 -1.1 3,567,490 - Psyr_2974 0.88 +2.4 3,567,686 + -1.2 3,567,686 + -2.9 3,567,695 + +2.4 3,567,701 + -1.6 3,567,702 - -0.4 3,567,753 - -1.0 3,567,832 + +2.9 3,567,832 + +2.9 3,567,833 - -0.2 3,567,888 + Psyr_2975 0.17 +0.9 3,567,889 - Psyr_2975 0.17 +0.4 3,568,028 + Psyr_2975 0.41 +0.4 3,568,028 + Psyr_2975 0.41 +0.4 3,568,029 - Psyr_2975 0.42 +3.3 3,568,078 - Psyr_2975 0.50 -1.4 3,568,078 - Psyr_2975 0.50 +1.4 3,568,078 - Psyr_2975 0.50 -1.4 3,568,078 - Psyr_2975 0.50 +1.6 3,568,078 - Psyr_2975 0.50 +1.7 3,568,152 + Psyr_2975 0.63 +0.1 3,568,152 + Psyr_2975 0.63 -0.5 3,568,152 + Psyr_2975 0.63 +2.9 3,568,152 + Psyr_2975 0.63 -0.1 3,568,152 + Psyr_2975 0.63 +2.9 3,568,152 + Psyr_2975 0.63 +0.6 3,568,153 - Psyr_2975 0.64 -1.2 3,568,153 - Psyr_2975 0.64 -0.6 3,568,153 - Psyr_2975 0.64 -0.9 3,568,153 - Psyr_2975 0.64 +1.2 3,568,185 + Psyr_2975 0.69 -0.1 3,568,185 + Psyr_2975 0.69 -1.3 3,568,185 + Psyr_2975 0.69 +0.1 3,568,185 + Psyr_2975 0.69 +0.4 3,568,186 - Psyr_2975 0.70 -1.0 3,568,220 + Psyr_2975 0.76 +0.4 3,568,250 + Psyr_2975 0.81 +0.4 3,568,251 - Psyr_2975 0.81 -0.4 3,568,335 - +0.3 3,568,335 - -2.9 3,568,351 + +1.2 3,568,361 + -1.7 3,568,361 + +2.2 3,568,361 + +0.9 3,568,362 - +0.2 3,568,362 - +1.5 3,568,362 - -2.8 3,568,362 - -3.1 3,568,362 - -2.4 3,568,363 + -0.2 3,568,364 - +1.4 3,568,364 - -2.2 3,568,364 - -2.7 3,568,410 + -0.2 3,568,410 + -2.9 3,568,411 - -2.4 3,568,411 - -2.1 3,568,482 + -2.3 3,568,551 - +1.1 3,568,551 - -3.0 3,568,582 + Psyr_2976 0.12 -2.2 3,568,582 + Psyr_2976 0.12 +0.8 3,568,583 - Psyr_2976 0.12 -2.7 3,568,583 - Psyr_2976 0.12 +0.7 3,568,586 + Psyr_2976 0.12 -0.8 3,568,586 + Psyr_2976 0.12 -0.3 3,568,586 + Psyr_2976 0.12 -0.5 3,568,586 + Psyr_2976 0.12 +0.7 3,568,586 + Psyr_2976 0.12 -2.1 3,568,586 + Psyr_2976 0.12 +1.7 3,568,586 + Psyr_2976 0.12 -1.2 3,568,586 + Psyr_2976 0.12 -2.5 3,568,586 + Psyr_2976 0.12 -2.6 3,568,586 + Psyr_2976 0.12 -0.5 3,568,587 - Psyr_2976 0.12 -0.4 3,568,587 - Psyr_2976 0.12 -1.0 3,568,587 - Psyr_2976 0.12 -2.6 3,568,587 - Psyr_2976 0.12 -1.5 3,568,664 + Psyr_2976 0.20 -2.4 3,568,664 + Psyr_2976 0.20 -2.8 3,568,665 - Psyr_2976 0.21 -2.6 3,568,747 + Psyr_2976 0.29 -1.6 3,568,747 + Psyr_2976 0.29 +1.9 3,568,748 - Psyr_2976 0.29 -0.5 3,568,748 - Psyr_2976 0.29 -2.0 3,568,748 - Psyr_2976 0.29 -0.3 3,568,752 + Psyr_2976 0.30 -1.2 3,568,816 + Psyr_2976 0.36 -0.9 3,568,816 + Psyr_2976 0.36 -2.9 3,568,816 + Psyr_2976 0.36 -2.3 3,568,816 + Psyr_2976 0.36 -1.6 3,568,816 + Psyr_2976 0.36 +0.1 3,568,816 + Psyr_2976 0.36 -2.5 3,568,816 + Psyr_2976 0.36 -1.0 3,568,816 + Psyr_2976 0.36 +1.2 3,568,816 + Psyr_2976 0.36 -1.9 3,568,816 + Psyr_2976 0.36 -1.1 3,568,816 + Psyr_2976 0.36 +1.1 3,568,816 + Psyr_2976 0.36 -0.9 3,568,816 + Psyr_2976 0.36 -0.8 3,568,816 + Psyr_2976 0.36 +0.3 3,568,817 - Psyr_2976 0.36 -1.5 3,568,817 - Psyr_2976 0.36 -1.8 3,568,817 - Psyr_2976 0.36 -2.3 3,568,817 - Psyr_2976 0.36 -3.7 3,568,817 - Psyr_2976 0.36 -2.0 3,568,817 - Psyr_2976 0.36 +0.1 3,568,817 - Psyr_2976 0.36 -2.1 3,568,817 - Psyr_2976 0.36 -0.0 3,568,817 - Psyr_2976 0.36 -1.3 3,568,817 - Psyr_2976 0.36 -2.2 3,568,817 - Psyr_2976 0.36 +0.9 3,568,817 - Psyr_2976 0.36 -1.2 3,568,817 - Psyr_2976 0.36 -1.8 3,568,817 - Psyr_2976 0.36 -2.6 3,568,818 + Psyr_2976 0.36 -1.0 3,568,819 - Psyr_2976 0.37 +1.2 3,568,819 - Psyr_2976 0.37 -2.7 3,568,820 + Psyr_2976 0.37 -1.2 3,568,820 + Psyr_2976 0.37 -0.1 3,568,820 + Psyr_2976 0.37 +0.3 3,568,820 + Psyr_2976 0.37 -1.0 3,568,821 - Psyr_2976 0.37 +0.6 3,568,821 - Psyr_2976 0.37 -1.6 3,568,821 - Psyr_2976 0.37 -0.4 3,568,821 - Psyr_2976 0.37 -0.5 3,568,821 - Psyr_2976 0.37 -0.9 3,568,821 - Psyr_2976 0.37 -1.7 3,568,821 - Psyr_2976 0.37 -2.5 3,568,821 - Psyr_2976 0.37 -2.8 3,568,863 - Psyr_2976 0.41 -2.5 3,568,907 + Psyr_2976 0.46 -0.8 3,568,907 + Psyr_2976 0.46 +0.8 3,568,907 + Psyr_2976 0.46 -2.9 3,568,908 - Psyr_2976 0.46 -0.1 3,568,908 - Psyr_2976 0.46 -2.8 3,568,908 - Psyr_2976 0.46 +0.1 3,568,908 - Psyr_2976 0.46 -0.7 3,568,948 + Psyr_2976 0.50 +0.1 3,568,949 - Psyr_2976 0.50 -4.2 3,568,949 - Psyr_2976 0.50 -0.9 3,569,111 - Psyr_2976 0.67 -2.3 3,569,408 + -2.2 3,569,499 - +4.0 3,569,521 - -1.2 3,569,561 + -2.0 3,569,595 - -2.0 3,569,621 + -0.4 3,569,633 + -0.1 3,569,634 - +1.3 3,569,693 + +0.1 3,569,710 - Psyr_2977 0.10 +1.3 3,569,713 - Psyr_2977 0.11 -1.7 3,569,740 + Psyr_2977 0.13 -0.6 3,569,851 - Psyr_2977 0.24 -0.5 3,570,020 + Psyr_2977 0.41 +4.2 3,570,020 + Psyr_2977 0.41 +2.4 3,570,021 - Psyr_2977 0.41 -0.3 3,570,021 - Psyr_2977 0.41 -2.7 3,570,021 - Psyr_2977 0.41 +1.7
Or see this region's nucleotide sequence