Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2705 and Psyr_2706 are separated by 64 nucleotides Psyr_2706 and Psyr_2707 are separated by 127 nucleotides Psyr_2707 and Psyr_2708 are separated by 145 nucleotides
Psyr_2705: Psyr_2705 - Helix-turn-helix motif protein, at 3,286,524 to 3,287,135
_2705
Psyr_2706: Psyr_2706 - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 3,287,200 to 3,287,949
_2706
Psyr_2707: Psyr_2707 - transcriptional regulator, LysR family, at 3,288,077 to 3,288,964
_2707
Psyr_2708: Psyr_2708 - Rieske [2Fe-2S] region, at 3,289,110 to 3,290,174
_2708
Position (kb)
3288
3289 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3287.081 kb on + strand at 3287.081 kb on + strand at 3287.081 kb on + strand at 3287.082 kb on - strand at 3287.082 kb on - strand at 3287.082 kb on - strand at 3287.161 kb on + strand at 3287.161 kb on + strand at 3287.162 kb on - strand at 3287.219 kb on + strand at 3287.219 kb on + strand at 3287.219 kb on + strand at 3287.220 kb on - strand at 3287.501 kb on + strand, within Psyr_2706 at 3287.502 kb on - strand, within Psyr_2706 at 3287.502 kb on - strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.689 kb on + strand, within Psyr_2706 at 3287.690 kb on - strand, within Psyr_2706 at 3287.690 kb on - strand, within Psyr_2706 at 3287.690 kb on - strand, within Psyr_2706 at 3287.729 kb on + strand, within Psyr_2706 at 3287.729 kb on + strand, within Psyr_2706 at 3287.729 kb on + strand, within Psyr_2706 at 3287.730 kb on - strand, within Psyr_2706 at 3287.730 kb on - strand, within Psyr_2706 at 3287.730 kb on - strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.867 kb on + strand, within Psyr_2706 at 3287.868 kb on - strand, within Psyr_2706 at 3287.868 kb on - strand, within Psyr_2706 at 3287.868 kb on - strand, within Psyr_2706 at 3287.868 kb on - strand, within Psyr_2706 at 3287.894 kb on + strand at 3287.894 kb on + strand at 3287.894 kb on + strand at 3287.895 kb on - strand at 3287.895 kb on - strand at 3287.895 kb on - strand at 3287.895 kb on - strand at 3287.949 kb on + strand at 3287.949 kb on + strand at 3287.949 kb on + strand at 3287.950 kb on - strand at 3287.950 kb on - strand at 3287.950 kb on - strand at 3287.950 kb on - strand at 3288.174 kb on + strand, within Psyr_2707 at 3288.175 kb on - strand, within Psyr_2707 at 3288.175 kb on - strand at 3288.175 kb on - strand, within Psyr_2707 at 3288.255 kb on + strand, within Psyr_2707 at 3288.398 kb on - strand, within Psyr_2707 at 3288.398 kb on - strand, within Psyr_2707 at 3288.405 kb on + strand, within Psyr_2707 at 3288.405 kb on + strand, within Psyr_2707 at 3288.405 kb on + strand, within Psyr_2707 at 3288.405 kb on + strand, within Psyr_2707 at 3288.406 kb on - strand, within Psyr_2707 at 3288.408 kb on + strand, within Psyr_2707 at 3288.409 kb on - strand, within Psyr_2707 at 3288.437 kb on + strand, within Psyr_2707 at 3288.448 kb on + strand, within Psyr_2707 at 3288.449 kb on - strand, within Psyr_2707 at 3288.449 kb on - strand, within Psyr_2707 at 3288.526 kb on + strand, within Psyr_2707 at 3288.572 kb on + strand, within Psyr_2707 at 3288.572 kb on + strand, within Psyr_2707 at 3288.572 kb on + strand, within Psyr_2707 at 3288.706 kb on - strand, within Psyr_2707 at 3288.706 kb on - strand, within Psyr_2707 at 3288.706 kb on - strand, within Psyr_2707 at 3288.948 kb on + strand at 3289.016 kb on + strand at 3289.016 kb on + strand at 3289.017 kb on - strand at 3289.036 kb on + strand at 3289.132 kb on - strand at 3289.207 kb on - strand at 3289.281 kb on + strand, within Psyr_2708 at 3289.281 kb on + strand, within Psyr_2708 at 3289.281 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.584 kb on + strand, within Psyr_2708 at 3289.585 kb on - strand, within Psyr_2708 at 3289.912 kb on - strand, within Psyr_2708 at 3289.912 kb on - strand, within Psyr_2708
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 3,287,081 + -1.6 3,287,081 + -0.9 3,287,081 + -2.4 3,287,082 - -0.3 3,287,082 - -1.8 3,287,082 - -1.8 3,287,161 + +2.0 3,287,161 + -1.4 3,287,162 - -1.1 3,287,219 + -1.0 3,287,219 + +0.3 3,287,219 + -0.0 3,287,220 - -0.8 3,287,501 + Psyr_2706 0.40 -2.6 3,287,502 - Psyr_2706 0.40 -1.5 3,287,502 - Psyr_2706 0.40 -1.3 3,287,689 + Psyr_2706 0.65 -1.7 3,287,689 + Psyr_2706 0.65 -0.5 3,287,689 + Psyr_2706 0.65 +0.8 3,287,689 + Psyr_2706 0.65 -2.5 3,287,689 + Psyr_2706 0.65 -1.8 3,287,690 - Psyr_2706 0.65 -1.8 3,287,690 - Psyr_2706 0.65 +4.1 3,287,690 - Psyr_2706 0.65 -0.8 3,287,729 + Psyr_2706 0.71 +0.1 3,287,729 + Psyr_2706 0.71 +1.6 3,287,729 + Psyr_2706 0.71 -0.5 3,287,730 - Psyr_2706 0.71 +1.3 3,287,730 - Psyr_2706 0.71 +0.2 3,287,730 - Psyr_2706 0.71 -0.3 3,287,867 + Psyr_2706 0.89 -2.3 3,287,867 + Psyr_2706 0.89 +0.5 3,287,867 + Psyr_2706 0.89 +0.3 3,287,867 + Psyr_2706 0.89 -0.7 3,287,867 + Psyr_2706 0.89 -2.1 3,287,868 - Psyr_2706 0.89 +2.7 3,287,868 - Psyr_2706 0.89 -0.9 3,287,868 - Psyr_2706 0.89 +3.6 3,287,868 - Psyr_2706 0.89 +0.0 3,287,894 + -1.5 3,287,894 + +0.6 3,287,894 + -2.8 3,287,895 - +2.8 3,287,895 - +1.3 3,287,895 - +1.1 3,287,895 - +1.2 3,287,949 + +2.0 3,287,949 + -1.2 3,287,949 + -1.1 3,287,950 - +0.6 3,287,950 - -0.9 3,287,950 - +1.5 3,287,950 - -0.3 3,288,174 + Psyr_2707 0.11 -3.0 3,288,175 - Psyr_2707 0.11 +1.9 3,288,175 - -0.3 3,288,175 - Psyr_2707 0.11 -1.5 3,288,255 + Psyr_2707 0.20 -1.1 3,288,398 - Psyr_2707 0.36 +0.6 3,288,398 - Psyr_2707 0.36 -1.5 3,288,405 + Psyr_2707 0.37 -1.0 3,288,405 + Psyr_2707 0.37 -0.2 3,288,405 + Psyr_2707 0.37 -2.5 3,288,405 + Psyr_2707 0.37 -0.0 3,288,406 - Psyr_2707 0.37 +1.2 3,288,408 + Psyr_2707 0.37 -1.5 3,288,409 - Psyr_2707 0.37 +0.4 3,288,437 + Psyr_2707 0.41 +0.2 3,288,448 + Psyr_2707 0.42 +3.5 3,288,449 - Psyr_2707 0.42 +0.2 3,288,449 - Psyr_2707 0.42 -0.0 3,288,526 + Psyr_2707 0.51 +0.0 3,288,572 + Psyr_2707 0.56 +1.0 3,288,572 + Psyr_2707 0.56 -0.1 3,288,572 + Psyr_2707 0.56 -2.7 3,288,706 - Psyr_2707 0.71 -0.3 3,288,706 - Psyr_2707 0.71 +1.8 3,288,706 - Psyr_2707 0.71 -2.9 3,288,948 + -1.2 3,289,016 + -1.5 3,289,016 + -0.9 3,289,017 - +0.9 3,289,036 + -0.1 3,289,132 - -1.3 3,289,207 - +0.5 3,289,281 + Psyr_2708 0.16 -0.8 3,289,281 + Psyr_2708 0.16 +1.9 3,289,281 + Psyr_2708 0.16 +0.1 3,289,584 + Psyr_2708 0.45 +1.0 3,289,584 + Psyr_2708 0.45 -2.2 3,289,584 + Psyr_2708 0.45 -0.1 3,289,584 + Psyr_2708 0.45 +0.0 3,289,584 + Psyr_2708 0.45 -1.0 3,289,585 - Psyr_2708 0.45 +0.4 3,289,912 - Psyr_2708 0.75 -1.6 3,289,912 - Psyr_2708 0.75 +1.4
Or see this region's nucleotide sequence