Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2650 and Psyr_2651 are separated by 11 nucleotides Psyr_2651 and Psyr_2652 overlap by 14 nucleotides Psyr_2652 and Psyr_2653 are separated by 266 nucleotides Psyr_2653 and Psyr_2654 are separated by 29 nucleotides
Psyr_2650: Psyr_2650 - hypothetical protein, at 3,221,945 to 3,222,412
_2650
Psyr_2651: Psyr_2651 - conserved hypothetical protein, at 3,222,424 to 3,223,383
_2651
Psyr_2652: Psyr_2652 - hypothetical protein, at 3,223,370 to 3,224,044
_2652
Psyr_2653: Psyr_2653 - ISPsy8, transposase OrfA, at 3,224,311 to 3,224,826
_2653
Psyr_2654: Psyr_2654 - Integrase, catalytic region, at 3,224,856 to 3,225,662
_2654
Position (kb)
3223
3224
3225 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 3222.387 kb on + strand at 3222.388 kb on - strand at 3222.401 kb on + strand at 3222.401 kb on + strand at 3222.402 kb on - strand at 3222.405 kb on + strand at 3222.405 kb on + strand at 3222.405 kb on + strand at 3222.502 kb on + strand at 3222.502 kb on + strand at 3222.517 kb on - strand at 3222.577 kb on - strand, within Psyr_2651 at 3222.577 kb on - strand, within Psyr_2651 at 3222.625 kb on + strand, within Psyr_2651 at 3222.625 kb on + strand, within Psyr_2651 at 3222.626 kb on - strand, within Psyr_2651 at 3222.626 kb on - strand, within Psyr_2651 at 3222.626 kb on - strand, within Psyr_2651 at 3222.688 kb on + strand at 3222.688 kb on + strand, within Psyr_2651 at 3222.688 kb on + strand, within Psyr_2651 at 3222.688 kb on + strand, within Psyr_2651 at 3222.688 kb on + strand, within Psyr_2651 at 3222.688 kb on + strand, within Psyr_2651 at 3222.688 kb on + strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.689 kb on - strand, within Psyr_2651 at 3222.718 kb on - strand, within Psyr_2651 at 3222.727 kb on + strand, within Psyr_2651 at 3222.728 kb on - strand, within Psyr_2651 at 3222.728 kb on - strand, within Psyr_2651 at 3223.134 kb on + strand, within Psyr_2651 at 3223.135 kb on - strand, within Psyr_2651 at 3223.135 kb on - strand, within Psyr_2651 at 3223.135 kb on - strand, within Psyr_2651 at 3223.165 kb on - strand, within Psyr_2651 at 3223.165 kb on - strand, within Psyr_2651 at 3223.165 kb on - strand, within Psyr_2651 at 3223.220 kb on + strand, within Psyr_2651 at 3223.220 kb on + strand, within Psyr_2651 at 3223.220 kb on + strand, within Psyr_2651 at 3223.221 kb on - strand, within Psyr_2651 at 3223.221 kb on - strand, within Psyr_2651 at 3223.221 kb on - strand at 3223.221 kb on - strand, within Psyr_2651 at 3223.221 kb on - strand, within Psyr_2651 at 3223.221 kb on - strand, within Psyr_2651 at 3223.221 kb on - strand, within Psyr_2651 at 3223.226 kb on - strand, within Psyr_2651 at 3223.392 kb on + strand at 3223.417 kb on + strand at 3223.417 kb on + strand at 3223.417 kb on + strand at 3223.417 kb on + strand at 3223.418 kb on - strand at 3223.418 kb on - strand at 3223.418 kb on - strand at 3223.418 kb on - strand at 3223.418 kb on - strand at 3223.418 kb on - strand at 3223.528 kb on + strand, within Psyr_2652 at 3223.529 kb on - strand, within Psyr_2652 at 3223.532 kb on + strand, within Psyr_2652 at 3223.532 kb on + strand, within Psyr_2652 at 3223.532 kb on + strand, within Psyr_2652 at 3223.532 kb on + strand, within Psyr_2652 at 3223.532 kb on + strand, within Psyr_2652 at 3223.533 kb on - strand, within Psyr_2652 at 3223.533 kb on - strand, within Psyr_2652 at 3223.533 kb on - strand, within Psyr_2652 at 3223.533 kb on - strand, within Psyr_2652 at 3223.533 kb on - strand, within Psyr_2652 at 3223.583 kb on + strand, within Psyr_2652 at 3223.584 kb on - strand, within Psyr_2652 at 3223.584 kb on - strand, within Psyr_2652 at 3223.584 kb on - strand, within Psyr_2652 at 3223.606 kb on + strand, within Psyr_2652 at 3223.606 kb on + strand, within Psyr_2652 at 3223.606 kb on + strand, within Psyr_2652 at 3223.607 kb on - strand, within Psyr_2652 at 3223.609 kb on - strand, within Psyr_2652 at 3223.658 kb on - strand, within Psyr_2652 at 3223.706 kb on + strand, within Psyr_2652 at 3223.706 kb on + strand, within Psyr_2652 at 3223.706 kb on + strand, within Psyr_2652 at 3223.706 kb on + strand, within Psyr_2652 at 3223.706 kb on + strand, within Psyr_2652 at 3223.707 kb on - strand, within Psyr_2652 at 3223.707 kb on - strand, within Psyr_2652 at 3223.707 kb on - strand, within Psyr_2652 at 3223.707 kb on - strand, within Psyr_2652 at 3223.707 kb on - strand, within Psyr_2652 at 3223.707 kb on - strand, within Psyr_2652 at 3223.780 kb on + strand, within Psyr_2652 at 3223.780 kb on + strand, within Psyr_2652 at 3223.781 kb on - strand, within Psyr_2652 at 3223.852 kb on + strand, within Psyr_2652 at 3223.904 kb on + strand, within Psyr_2652 at 3223.905 kb on - strand, within Psyr_2652 at 3223.905 kb on - strand, within Psyr_2652 at 3224.036 kb on + strand at 3224.037 kb on - strand at 3224.037 kb on - strand at 3224.051 kb on + strand at 3224.052 kb on - strand at 3224.052 kb on - strand at 3224.052 kb on - strand at 3224.052 kb on - strand at 3224.052 kb on - strand at 3224.052 kb on - strand at 3224.073 kb on - strand at 3224.115 kb on + strand at 3224.136 kb on - strand at 3224.136 kb on - strand at 3224.226 kb on + strand at 3224.227 kb on - strand at 3224.227 kb on - strand at 3224.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 3,222,387 + +0.9 3,222,388 - +3.2 3,222,401 + +0.1 3,222,401 + -0.0 3,222,402 - -2.3 3,222,405 + +1.2 3,222,405 + +0.4 3,222,405 + -2.3 3,222,502 + -0.9 3,222,502 + +0.1 3,222,517 - +3.3 3,222,577 - Psyr_2651 0.16 -1.3 3,222,577 - Psyr_2651 0.16 +0.0 3,222,625 + Psyr_2651 0.21 +2.9 3,222,625 + Psyr_2651 0.21 +2.9 3,222,626 - Psyr_2651 0.21 -1.6 3,222,626 - Psyr_2651 0.21 +0.6 3,222,626 - Psyr_2651 0.21 -2.1 3,222,688 + -0.1 3,222,688 + Psyr_2651 0.28 -0.7 3,222,688 + Psyr_2651 0.28 +0.4 3,222,688 + Psyr_2651 0.28 -1.7 3,222,688 + Psyr_2651 0.28 +0.1 3,222,688 + Psyr_2651 0.28 -0.8 3,222,688 + Psyr_2651 0.28 +1.3 3,222,689 - Psyr_2651 0.28 -0.4 3,222,689 - Psyr_2651 0.28 -2.1 3,222,689 - Psyr_2651 0.28 -1.0 3,222,689 - Psyr_2651 0.28 -1.0 3,222,689 - Psyr_2651 0.28 -2.2 3,222,689 - Psyr_2651 0.28 -1.3 3,222,689 - Psyr_2651 0.28 -1.8 3,222,689 - Psyr_2651 0.28 -1.3 3,222,689 - Psyr_2651 0.28 +0.0 3,222,718 - Psyr_2651 0.31 -0.5 3,222,727 + Psyr_2651 0.32 +0.9 3,222,728 - Psyr_2651 0.32 +1.1 3,222,728 - Psyr_2651 0.32 -0.9 3,223,134 + Psyr_2651 0.74 +0.3 3,223,135 - Psyr_2651 0.74 +0.8 3,223,135 - Psyr_2651 0.74 -2.6 3,223,135 - Psyr_2651 0.74 -1.3 3,223,165 - Psyr_2651 0.77 +1.9 3,223,165 - Psyr_2651 0.77 -0.6 3,223,165 - Psyr_2651 0.77 +0.9 3,223,220 + Psyr_2651 0.83 -3.5 3,223,220 + Psyr_2651 0.83 +2.2 3,223,220 + Psyr_2651 0.83 -2.1 3,223,221 - Psyr_2651 0.83 -1.9 3,223,221 - Psyr_2651 0.83 +1.2 3,223,221 - +1.9 3,223,221 - Psyr_2651 0.83 +0.1 3,223,221 - Psyr_2651 0.83 -0.1 3,223,221 - Psyr_2651 0.83 -0.3 3,223,221 - Psyr_2651 0.83 +3.3 3,223,226 - Psyr_2651 0.84 -0.4 3,223,392 + +1.7 3,223,417 + +0.9 3,223,417 + +1.9 3,223,417 + -1.1 3,223,417 + -3.0 3,223,418 - +2.9 3,223,418 - +0.3 3,223,418 - -2.0 3,223,418 - -0.3 3,223,418 - +1.9 3,223,418 - +2.5 3,223,528 + Psyr_2652 0.23 -0.3 3,223,529 - Psyr_2652 0.24 +1.3 3,223,532 + Psyr_2652 0.24 -1.8 3,223,532 + Psyr_2652 0.24 -2.0 3,223,532 + Psyr_2652 0.24 +1.0 3,223,532 + Psyr_2652 0.24 +0.7 3,223,532 + Psyr_2652 0.24 -1.3 3,223,533 - Psyr_2652 0.24 +1.9 3,223,533 - Psyr_2652 0.24 +1.9 3,223,533 - Psyr_2652 0.24 -0.3 3,223,533 - Psyr_2652 0.24 +1.9 3,223,533 - Psyr_2652 0.24 +2.1 3,223,583 + Psyr_2652 0.32 +2.8 3,223,584 - Psyr_2652 0.32 +2.9 3,223,584 - Psyr_2652 0.32 +0.3 3,223,584 - Psyr_2652 0.32 +1.9 3,223,606 + Psyr_2652 0.35 +1.2 3,223,606 + Psyr_2652 0.35 +2.2 3,223,606 + Psyr_2652 0.35 -2.4 3,223,607 - Psyr_2652 0.35 +0.6 3,223,609 - Psyr_2652 0.35 -0.4 3,223,658 - Psyr_2652 0.43 +1.3 3,223,706 + Psyr_2652 0.50 -0.1 3,223,706 + Psyr_2652 0.50 +0.4 3,223,706 + Psyr_2652 0.50 -3.2 3,223,706 + Psyr_2652 0.50 +2.5 3,223,706 + Psyr_2652 0.50 +0.3 3,223,707 - Psyr_2652 0.50 +0.9 3,223,707 - Psyr_2652 0.50 +0.9 3,223,707 - Psyr_2652 0.50 +2.1 3,223,707 - Psyr_2652 0.50 -0.9 3,223,707 - Psyr_2652 0.50 -1.8 3,223,707 - Psyr_2652 0.50 -2.1 3,223,780 + Psyr_2652 0.61 -0.1 3,223,780 + Psyr_2652 0.61 -2.5 3,223,781 - Psyr_2652 0.61 +2.5 3,223,852 + Psyr_2652 0.71 +0.6 3,223,904 + Psyr_2652 0.79 -1.6 3,223,905 - Psyr_2652 0.79 -1.0 3,223,905 - Psyr_2652 0.79 +4.1 3,224,036 + +6.3 3,224,037 - -1.1 3,224,037 - -1.3 3,224,051 + -0.3 3,224,052 - -0.8 3,224,052 - +0.9 3,224,052 - -3.5 3,224,052 - -1.8 3,224,052 - -0.1 3,224,052 - +0.5 3,224,073 - -0.2 3,224,115 + -3.9 3,224,136 - +1.0 3,224,136 - -1.7 3,224,226 + -2.0 3,224,227 - +1.4 3,224,227 - -0.3 3,224,296 - +0.3
Or see this region's nucleotide sequence