Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2277 and Psyr_2278 are separated by 546 nucleotides Psyr_2278 and Psyr_2279 are separated by 206 nucleotides Psyr_2279 and Psyr_2280 are separated by 323 nucleotides
Psyr_2277: Psyr_2277 - ammonium transporter, at 2,643,672 to 2,644,997
_2277
Psyr_2278: Psyr_2278 - luciferase, at 2,645,544 to 2,646,581
_2278
Psyr_2279: Psyr_2279 - conserved hypothetical protein, at 2,646,788 to 2,647,123
_2279
Psyr_2280: Psyr_2280 - Alkanesulfonate monooxygenase, at 2,647,447 to 2,648,547
_2280
Position (kb)
2645
2646
2647 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2644.704 kb on + strand, within Psyr_2277 at 2644.763 kb on - strand, within Psyr_2277 at 2644.986 kb on + strand at 2644.987 kb on - strand at 2645.041 kb on + strand at 2645.041 kb on + strand at 2645.042 kb on - strand at 2645.042 kb on - strand at 2645.042 kb on - strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.043 kb on + strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.044 kb on - strand at 2645.272 kb on + strand at 2645.318 kb on - strand at 2645.367 kb on + strand at 2645.494 kb on + strand at 2645.494 kb on + strand at 2645.660 kb on - strand, within Psyr_2278 at 2645.702 kb on + strand, within Psyr_2278 at 2645.703 kb on - strand, within Psyr_2278 at 2645.703 kb on - strand, within Psyr_2278 at 2645.703 kb on - strand, within Psyr_2278 at 2645.828 kb on - strand, within Psyr_2278 at 2646.146 kb on - strand, within Psyr_2278 at 2646.169 kb on - strand, within Psyr_2278 at 2646.169 kb on - strand, within Psyr_2278 at 2646.397 kb on + strand, within Psyr_2278 at 2646.434 kb on - strand, within Psyr_2278 at 2646.434 kb on - strand, within Psyr_2278 at 2646.434 kb on - strand, within Psyr_2278 at 2646.588 kb on + strand at 2646.588 kb on + strand at 2646.588 kb on + strand at 2646.588 kb on + strand at 2646.589 kb on - strand at 2646.597 kb on + strand at 2646.646 kb on - strand at 2646.812 kb on - strand at 2646.812 kb on - strand at 2646.813 kb on + strand at 2646.813 kb on + strand at 2646.814 kb on - strand at 2646.814 kb on - strand at 2646.814 kb on - strand at 2646.814 kb on - strand at 2646.862 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.928 kb on + strand, within Psyr_2279 at 2646.929 kb on - strand, within Psyr_2279 at 2646.930 kb on + strand, within Psyr_2279 at 2646.930 kb on + strand, within Psyr_2279 at 2646.930 kb on + strand, within Psyr_2279 at 2646.931 kb on - strand, within Psyr_2279 at 2646.931 kb on - strand, within Psyr_2279 at 2646.931 kb on - strand, within Psyr_2279 at 2646.945 kb on + strand, within Psyr_2279 at 2646.945 kb on + strand, within Psyr_2279 at 2646.945 kb on + strand, within Psyr_2279 at 2646.946 kb on - strand, within Psyr_2279 at 2646.946 kb on - strand, within Psyr_2279 at 2646.946 kb on - strand, within Psyr_2279 at 2646.946 kb on - strand, within Psyr_2279 at 2647.052 kb on + strand, within Psyr_2279 at 2647.194 kb on - strand at 2647.408 kb on + strand at 2647.447 kb on - strand at 2647.546 kb on + strand at 2647.547 kb on - strand at 2647.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 2,644,704 + Psyr_2277 0.78 +1.8 2,644,763 - Psyr_2277 0.82 +1.4 2,644,986 + -2.1 2,644,987 - -1.0 2,645,041 + +1.9 2,645,041 + -2.0 2,645,042 - -2.3 2,645,042 - -1.7 2,645,042 - -0.8 2,645,043 + -1.5 2,645,043 + -0.9 2,645,043 + +1.4 2,645,043 + +0.4 2,645,043 + -2.5 2,645,043 + -1.7 2,645,043 + -0.8 2,645,043 + -0.4 2,645,043 + -1.5 2,645,043 + +1.2 2,645,044 - +0.3 2,645,044 - +0.4 2,645,044 - -3.1 2,645,044 - +2.1 2,645,044 - -0.1 2,645,044 - +2.3 2,645,044 - +2.4 2,645,044 - +2.4 2,645,272 + +3.5 2,645,318 - -0.7 2,645,367 + -1.4 2,645,494 + -1.7 2,645,494 + -0.3 2,645,660 - Psyr_2278 0.11 -3.2 2,645,702 + Psyr_2278 0.15 +2.9 2,645,703 - Psyr_2278 0.15 -2.5 2,645,703 - Psyr_2278 0.15 -1.5 2,645,703 - Psyr_2278 0.15 -1.1 2,645,828 - Psyr_2278 0.27 -0.8 2,646,146 - Psyr_2278 0.58 -2.6 2,646,169 - Psyr_2278 0.60 +1.9 2,646,169 - Psyr_2278 0.60 -1.5 2,646,397 + Psyr_2278 0.82 -1.1 2,646,434 - Psyr_2278 0.86 +0.3 2,646,434 - Psyr_2278 0.86 +0.7 2,646,434 - Psyr_2278 0.86 -1.1 2,646,588 + -2.3 2,646,588 + +0.4 2,646,588 + +0.3 2,646,588 + +0.3 2,646,589 - -1.6 2,646,597 + +1.6 2,646,646 - +1.5 2,646,812 - -1.3 2,646,812 - -2.9 2,646,813 + +3.9 2,646,813 + -1.2 2,646,814 - -1.6 2,646,814 - +0.2 2,646,814 - -3.3 2,646,814 - +1.6 2,646,862 + Psyr_2279 0.22 +1.0 2,646,928 + Psyr_2279 0.42 +0.4 2,646,928 + Psyr_2279 0.42 +2.4 2,646,928 + Psyr_2279 0.42 -3.0 2,646,928 + Psyr_2279 0.42 +0.9 2,646,928 + Psyr_2279 0.42 +1.1 2,646,929 - Psyr_2279 0.42 +0.1 2,646,930 + Psyr_2279 0.42 +1.1 2,646,930 + Psyr_2279 0.42 +1.5 2,646,930 + Psyr_2279 0.42 -1.9 2,646,931 - Psyr_2279 0.43 +1.6 2,646,931 - Psyr_2279 0.43 +0.6 2,646,931 - Psyr_2279 0.43 +2.6 2,646,945 + Psyr_2279 0.47 +0.2 2,646,945 + Psyr_2279 0.47 -2.3 2,646,945 + Psyr_2279 0.47 +0.4 2,646,946 - Psyr_2279 0.47 +1.6 2,646,946 - Psyr_2279 0.47 +1.6 2,646,946 - Psyr_2279 0.47 +0.5 2,646,946 - Psyr_2279 0.47 -1.2 2,647,052 + Psyr_2279 0.79 +0.2 2,647,194 - +1.6 2,647,408 + -2.0 2,647,447 - +0.6 2,647,546 + +1.0 2,647,547 - -0.3 2,647,547 - -1.9
Or see this region's nucleotide sequence