Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1971 and Psyr_1972 are separated by 52 nucleotides Psyr_1972 and Psyr_1973 are separated by 271 nucleotides
Psyr_1971: Psyr_1971 - Peptidase S45, penicillin amidase, at 2,285,834 to 2,288,173
_1971
Psyr_1972: Psyr_1972 - conserved hypothetical protein, at 2,288,226 to 2,288,537
_1972
Psyr_1973: Psyr_1973 - Aspartate transaminase, at 2,288,809 to 2,290,005
_1973
Position (kb)
2288
2289 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2287.250 kb on + strand, within Psyr_1971 at 2287.250 kb on + strand, within Psyr_1971 at 2287.487 kb on + strand, within Psyr_1971 at 2287.632 kb on - strand, within Psyr_1971 at 2287.819 kb on + strand, within Psyr_1971 at 2287.898 kb on + strand, within Psyr_1971 at 2287.898 kb on + strand, within Psyr_1971 at 2287.898 kb on + strand, within Psyr_1971 at 2288.136 kb on - strand at 2288.283 kb on + strand, within Psyr_1972 at 2288.284 kb on - strand, within Psyr_1972 at 2288.284 kb on - strand, within Psyr_1972 at 2288.284 kb on - strand, within Psyr_1972 at 2288.323 kb on + strand, within Psyr_1972 at 2288.324 kb on - strand, within Psyr_1972 at 2288.532 kb on - strand at 2288.541 kb on + strand at 2288.542 kb on - strand at 2288.542 kb on - strand at 2288.542 kb on - strand at 2288.542 kb on - strand at 2288.657 kb on - strand at 2288.664 kb on - strand at 2288.664 kb on - strand at 2288.664 kb on - strand at 2288.671 kb on - strand at 2288.671 kb on - strand at 2288.671 kb on - strand at 2288.677 kb on + strand at 2288.677 kb on + strand at 2288.677 kb on + strand at 2288.678 kb on - strand at 2288.678 kb on - strand at 2288.684 kb on + strand at 2288.684 kb on + strand at 2288.691 kb on + strand at 2288.691 kb on + strand at 2288.692 kb on - strand at 2288.692 kb on - strand at 2288.705 kb on + strand at 2288.705 kb on + strand at 2288.705 kb on + strand at 2288.705 kb on + strand at 2288.839 kb on + strand at 2288.839 kb on + strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.840 kb on - strand at 2288.878 kb on - strand at 2288.897 kb on + strand at 2288.897 kb on + strand at 2288.898 kb on - strand at 2289.152 kb on + strand, within Psyr_1973 at 2289.152 kb on + strand, within Psyr_1973 at 2289.152 kb on + strand, within Psyr_1973 at 2289.249 kb on - strand, within Psyr_1973
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 2,287,250 + Psyr_1971 0.61 -3.3 2,287,250 + Psyr_1971 0.61 -0.9 2,287,487 + Psyr_1971 0.71 +0.8 2,287,632 - Psyr_1971 0.77 +2.3 2,287,819 + Psyr_1971 0.85 -1.0 2,287,898 + Psyr_1971 0.88 -0.1 2,287,898 + Psyr_1971 0.88 +1.2 2,287,898 + Psyr_1971 0.88 -0.6 2,288,136 - -2.4 2,288,283 + Psyr_1972 0.18 +0.2 2,288,284 - Psyr_1972 0.19 +0.3 2,288,284 - Psyr_1972 0.19 -2.8 2,288,284 - Psyr_1972 0.19 +1.5 2,288,323 + Psyr_1972 0.31 +0.1 2,288,324 - Psyr_1972 0.31 +1.0 2,288,532 - +0.3 2,288,541 + -1.0 2,288,542 - -0.8 2,288,542 - -0.4 2,288,542 - -1.6 2,288,542 - +0.6 2,288,657 - -3.0 2,288,664 - +0.1 2,288,664 - -0.6 2,288,664 - +0.4 2,288,671 - +0.1 2,288,671 - -0.6 2,288,671 - -0.1 2,288,677 + -0.8 2,288,677 + -0.2 2,288,677 + -2.3 2,288,678 - -1.3 2,288,678 - +0.3 2,288,684 + +1.8 2,288,684 + -0.3 2,288,691 + +0.7 2,288,691 + +0.1 2,288,692 - -0.4 2,288,692 - +0.3 2,288,705 + +1.0 2,288,705 + +2.3 2,288,705 + -2.7 2,288,705 + -2.0 2,288,839 + +1.2 2,288,839 + +2.3 2,288,840 - -1.2 2,288,840 - +0.6 2,288,840 - -3.0 2,288,840 - -2.2 2,288,840 - -0.5 2,288,878 - -0.3 2,288,897 + -3.2 2,288,897 + +0.8 2,288,898 - +2.3 2,289,152 + Psyr_1973 0.29 -2.8 2,289,152 + Psyr_1973 0.29 -3.0 2,289,152 + Psyr_1973 0.29 +2.7 2,289,249 - Psyr_1973 0.37 +1.3
Or see this region's nucleotide sequence