Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0967 and Psyr_0968 are separated by 177 nucleotides Psyr_0968 and Psyr_0969 are separated by 4 nucleotides Psyr_0969 and Psyr_0970 are separated by 207 nucleotides
Psyr_0967: Psyr_0967 - ferrochelatase, at 1,100,499 to 1,101,521
_0967
Psyr_0968: Psyr_0968 - Xanthine/uracil permease family, at 1,101,699 to 1,102,976
_0968
Psyr_0969: Psyr_0969 - uracil phosphoribosyltransferase, at 1,102,981 to 1,103,619
_0969
Psyr_0970: Psyr_0970 - Phosphoribosyltransferase, at 1,103,827 to 1,104,384
_0970
Position (kb)
1101
1102
1103 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.555 kb on + strand at 1101.840 kb on + strand, within Psyr_0968 at 1101.841 kb on - strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.079 kb on - strand, within Psyr_0968 at 1102.079 kb on - strand, within Psyr_0968 at 1102.117 kb on + strand, within Psyr_0968 at 1102.118 kb on - strand, within Psyr_0968 at 1102.118 kb on - strand, within Psyr_0968 at 1102.124 kb on + strand, within Psyr_0968 at 1102.387 kb on + strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.682 kb on + strand, within Psyr_0968 at 1102.683 kb on - strand, within Psyr_0968 at 1102.683 kb on - strand, within Psyr_0968 at 1102.683 kb on - strand, within Psyr_0968 at 1102.683 kb on - strand, within Psyr_0968 at 1102.684 kb on + strand, within Psyr_0968 at 1102.684 kb on + strand, within Psyr_0968 at 1102.684 kb on + strand, within Psyr_0968 at 1102.684 kb on + strand, within Psyr_0968 at 1102.685 kb on - strand, within Psyr_0968 at 1102.685 kb on - strand, within Psyr_0968 at 1103.045 kb on + strand, within Psyr_0969 at 1103.046 kb on - strand, within Psyr_0969 at 1103.046 kb on - strand, within Psyr_0969 at 1103.046 kb on - strand, within Psyr_0969 at 1103.084 kb on + strand, within Psyr_0969 at 1103.084 kb on + strand, within Psyr_0969 at 1103.085 kb on - strand, within Psyr_0969 at 1103.085 kb on - strand, within Psyr_0969 at 1103.282 kb on + strand, within Psyr_0969 at 1103.282 kb on + strand, within Psyr_0969 at 1103.465 kb on + strand, within Psyr_0969 at 1103.466 kb on - strand, within Psyr_0969 at 1103.466 kb on - strand, within Psyr_0969 at 1103.466 kb on - strand, within Psyr_0969 at 1103.466 kb on - strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.498 kb on + strand, within Psyr_0969 at 1103.499 kb on - strand, within Psyr_0969 at 1103.499 kb on - strand, within Psyr_0969 at 1103.499 kb on - strand, within Psyr_0969 at 1103.499 kb on - strand, within Psyr_0969 at 1103.569 kb on - strand at 1103.587 kb on + strand at 1103.588 kb on - strand at 1103.652 kb on + strand at 1103.652 kb on + strand at 1103.652 kb on + strand at 1103.652 kb on + strand at 1103.653 kb on - strand at 1103.653 kb on - strand at 1103.653 kb on - strand at 1103.653 kb on - strand at 1103.662 kb on + strand at 1103.663 kb on - strand at 1103.681 kb on + strand at 1103.682 kb on - strand at 1103.746 kb on + strand at 1103.783 kb on + strand at 1103.784 kb on - strand at 1103.812 kb on + strand at 1103.881 kb on + strand at 1103.882 kb on - strand at 1103.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 1,101,519 + +0.9 1,101,519 + -1.8 1,101,519 + +0.6 1,101,519 + -2.0 1,101,519 + +2.5 1,101,519 + +1.2 1,101,519 + -0.0 1,101,519 + +0.2 1,101,520 - +0.2 1,101,520 - -0.5 1,101,520 - -2.1 1,101,520 - +1.6 1,101,555 + -1.3 1,101,840 + Psyr_0968 0.11 +0.3 1,101,841 - Psyr_0968 0.11 +1.5 1,102,078 + Psyr_0968 0.30 -0.1 1,102,078 + Psyr_0968 0.30 -2.8 1,102,078 + Psyr_0968 0.30 -0.6 1,102,078 + Psyr_0968 0.30 -0.5 1,102,078 + Psyr_0968 0.30 -1.4 1,102,078 + Psyr_0968 0.30 +3.3 1,102,079 - Psyr_0968 0.30 +6.6 1,102,079 - Psyr_0968 0.30 -1.3 1,102,117 + Psyr_0968 0.33 -1.2 1,102,118 - Psyr_0968 0.33 -0.6 1,102,118 - Psyr_0968 0.33 +1.0 1,102,124 + Psyr_0968 0.33 +2.1 1,102,387 + Psyr_0968 0.54 -2.6 1,102,388 - Psyr_0968 0.54 -0.6 1,102,388 - Psyr_0968 0.54 +2.4 1,102,388 - Psyr_0968 0.54 -1.5 1,102,388 - Psyr_0968 0.54 +0.9 1,102,388 - Psyr_0968 0.54 +1.4 1,102,682 + Psyr_0968 0.77 +0.7 1,102,682 + Psyr_0968 0.77 +0.2 1,102,682 + Psyr_0968 0.77 -1.5 1,102,682 + Psyr_0968 0.77 -2.9 1,102,682 + Psyr_0968 0.77 -0.7 1,102,683 - Psyr_0968 0.77 -2.5 1,102,683 - Psyr_0968 0.77 -1.3 1,102,683 - Psyr_0968 0.77 +0.1 1,102,683 - Psyr_0968 0.77 -2.5 1,102,684 + Psyr_0968 0.77 -1.1 1,102,684 + Psyr_0968 0.77 +1.9 1,102,684 + Psyr_0968 0.77 +1.9 1,102,684 + Psyr_0968 0.77 +1.9 1,102,685 - Psyr_0968 0.77 -3.0 1,102,685 - Psyr_0968 0.77 -1.6 1,103,045 + Psyr_0969 0.10 +0.4 1,103,046 - Psyr_0969 0.10 -3.0 1,103,046 - Psyr_0969 0.10 +1.6 1,103,046 - Psyr_0969 0.10 -0.6 1,103,084 + Psyr_0969 0.16 -0.1 1,103,084 + Psyr_0969 0.16 -0.6 1,103,085 - Psyr_0969 0.16 -2.7 1,103,085 - Psyr_0969 0.16 -1.0 1,103,282 + Psyr_0969 0.47 +0.0 1,103,282 + Psyr_0969 0.47 +0.4 1,103,465 + Psyr_0969 0.76 -3.6 1,103,466 - Psyr_0969 0.76 +0.6 1,103,466 - Psyr_0969 0.76 -1.2 1,103,466 - Psyr_0969 0.76 +1.5 1,103,466 - Psyr_0969 0.76 -2.6 1,103,498 + Psyr_0969 0.81 +0.3 1,103,498 + Psyr_0969 0.81 +0.4 1,103,498 + Psyr_0969 0.81 -2.7 1,103,498 + Psyr_0969 0.81 +0.7 1,103,498 + Psyr_0969 0.81 +2.2 1,103,498 + Psyr_0969 0.81 +0.7 1,103,498 + Psyr_0969 0.81 -0.1 1,103,498 + Psyr_0969 0.81 +1.1 1,103,498 + Psyr_0969 0.81 +0.9 1,103,498 + Psyr_0969 0.81 -3.2 1,103,498 + Psyr_0969 0.81 -3.1 1,103,499 - Psyr_0969 0.81 +0.7 1,103,499 - Psyr_0969 0.81 -0.8 1,103,499 - Psyr_0969 0.81 +3.0 1,103,499 - Psyr_0969 0.81 -1.5 1,103,569 - -1.0 1,103,587 + -1.3 1,103,588 - +0.2 1,103,652 + -1.0 1,103,652 + -1.9 1,103,652 + -0.5 1,103,652 + -0.1 1,103,653 - +1.5 1,103,653 - -1.2 1,103,653 - -1.5 1,103,653 - +0.7 1,103,662 + -2.6 1,103,663 - -0.4 1,103,681 + -2.7 1,103,682 - +0.5 1,103,746 + -2.9 1,103,783 + -0.3 1,103,784 - +1.2 1,103,812 + +2.1 1,103,881 + +0.6 1,103,882 - +0.7 1,103,882 - +0.9
Or see this region's nucleotide sequence