Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0855 and Psyr_0856 are separated by 144 nucleotides Psyr_0856 and Psyr_0857 overlap by 8 nucleotides Psyr_0857 and Psyr_0858 are separated by 10 nucleotides Psyr_0858 and Psyr_0859 are separated by 131 nucleotides
Psyr_0855: Psyr_0855 - conserved hypothetical protein, at 974,997 to 976,274
_0855
Psyr_0856: Psyr_0856 - conserved hypothetical protein, at 976,419 to 977,225
_0856
Psyr_0857: Psyr_0857 - [SSU ribosomal protein S18P]-alanine acetyltransferase, at 977,218 to 977,670
_0857
Psyr_0858: Psyr_0858 - conserved hypothetical protein, at 977,681 to 978,088
_0858
Psyr_0859: Psyr_0859 - Carbonate dehydratase, at 978,220 to 978,858
_0859
Position (kb) 977
978 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 976.313 kb on + strand at 976.313 kb on + strand at 976.313 kb on + strand at 976.313 kb on + strand at 976.332 kb on + strand at 976.332 kb on + strand at 976.332 kb on + strand at 976.333 kb on - strand at 976.333 kb on - strand at 976.333 kb on - strand at 976.333 kb on - strand at 976.380 kb on + strand at 976.380 kb on + strand at 976.394 kb on + strand at 976.399 kb on + strand at 977.231 kb on + strand at 977.285 kb on - strand, within Psyr_0857 at 977.285 kb on - strand, within Psyr_0857 at 977.566 kb on + strand, within Psyr_0857 at 977.567 kb on - strand, within Psyr_0857 at 977.592 kb on - strand, within Psyr_0857 at 977.609 kb on - strand, within Psyr_0857 at 977.668 kb on + strand at 977.669 kb on - strand at 977.669 kb on - strand at 977.669 kb on - strand at 977.671 kb on + strand at 977.671 kb on + strand at 977.671 kb on + strand at 977.671 kb on + strand at 977.671 kb on + strand at 977.671 kb on + strand at 977.671 kb on + strand at 977.671 kb on + strand at 977.672 kb on - strand at 977.672 kb on - strand at 977.672 kb on - strand at 977.672 kb on - strand at 977.672 kb on - strand at 977.962 kb on + strand, within Psyr_0858 at 977.962 kb on + strand, within Psyr_0858 at 977.963 kb on - strand, within Psyr_0858 at 978.230 kb on + strand at 978.230 kb on + strand at 978.231 kb on - strand at 978.272 kb on + strand at 978.273 kb on - strand at 978.479 kb on + strand, within Psyr_0859 at 978.479 kb on + strand, within Psyr_0859 at 978.479 kb on + strand, within Psyr_0859 at 978.479 kb on + strand, within Psyr_0859 at 978.480 kb on - strand, within Psyr_0859 at 978.480 kb on - strand, within Psyr_0859 at 978.480 kb on - strand, within Psyr_0859 at 978.598 kb on + strand, within Psyr_0859 at 978.598 kb on + strand, within Psyr_0859 at 978.598 kb on + strand, within Psyr_0859 at 978.598 kb on + strand, within Psyr_0859 at 978.598 kb on + strand, within Psyr_0859 at 978.599 kb on - strand, within Psyr_0859 at 978.599 kb on - strand, within Psyr_0859 at 978.599 kb on - strand, within Psyr_0859 at 978.599 kb on - strand, within Psyr_0859 at 978.601 kb on + strand, within Psyr_0859
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 976,313 + -2.2 976,313 + +1.7 976,313 + +0.2 976,313 + +0.2 976,332 + +0.9 976,332 + -1.9 976,332 + -0.3 976,333 - -1.1 976,333 - -2.7 976,333 - -4.1 976,333 - -4.0 976,380 + +0.8 976,380 + -0.7 976,394 + +2.6 976,399 + -2.7 977,231 + -0.1 977,285 - Psyr_0857 0.15 +2.0 977,285 - Psyr_0857 0.15 +1.0 977,566 + Psyr_0857 0.77 +2.9 977,567 - Psyr_0857 0.77 +4.0 977,592 - Psyr_0857 0.83 -0.7 977,609 - Psyr_0857 0.86 -2.6 977,668 + +3.8 977,669 - -0.2 977,669 - -1.4 977,669 - -1.6 977,671 + +1.7 977,671 + +1.5 977,671 + -0.4 977,671 + +1.7 977,671 + +0.9 977,671 + -2.5 977,671 + -0.3 977,671 + -2.4 977,672 - +2.3 977,672 - +2.2 977,672 - +0.5 977,672 - -1.5 977,672 - +1.4 977,962 + Psyr_0858 0.69 -1.8 977,962 + Psyr_0858 0.69 -1.2 977,963 - Psyr_0858 0.69 +0.1 978,230 + +0.6 978,230 + -1.9 978,231 - +0.9 978,272 + -0.2 978,273 - -2.3 978,479 + Psyr_0859 0.41 -0.8 978,479 + Psyr_0859 0.41 -2.5 978,479 + Psyr_0859 0.41 -1.9 978,479 + Psyr_0859 0.41 -1.1 978,480 - Psyr_0859 0.41 -0.3 978,480 - Psyr_0859 0.41 +0.1 978,480 - Psyr_0859 0.41 -1.5 978,598 + Psyr_0859 0.59 -0.5 978,598 + Psyr_0859 0.59 +1.5 978,598 + Psyr_0859 0.59 -2.4 978,598 + Psyr_0859 0.59 -2.6 978,598 + Psyr_0859 0.59 +0.4 978,599 - Psyr_0859 0.59 +1.9 978,599 - Psyr_0859 0.59 -0.7 978,599 - Psyr_0859 0.59 -1.7 978,599 - Psyr_0859 0.59 +1.2 978,601 + Psyr_0859 0.60 -3.0
Or see this region's nucleotide sequence