Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0824 and Psyr_0825 are separated by 107 nucleotides Psyr_0825 and Psyr_0826 are separated by 383 nucleotides
Psyr_0824: Psyr_0824 - acetyl-CoA acetyltransferase, at 932,388 to 933,566
_0824
Psyr_0825: Psyr_0825 - conserved hypothetical protein, at 933,674 to 934,540
_0825
Psyr_0826: Psyr_0826 - glucose-6-phosphate isomerase, at 934,924 to 936,588
_0826
Position (kb) 933
934
935 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 932.783 kb on + strand, within Psyr_0824 at 933.064 kb on + strand, within Psyr_0824 at 933.065 kb on - strand, within Psyr_0824 at 933.065 kb on - strand, within Psyr_0824 at 933.103 kb on + strand, within Psyr_0824 at 933.103 kb on + strand, within Psyr_0824 at 933.103 kb on + strand, within Psyr_0824 at 933.104 kb on - strand, within Psyr_0824 at 933.199 kb on + strand, within Psyr_0824 at 933.199 kb on + strand, within Psyr_0824 at 933.199 kb on + strand, within Psyr_0824 at 933.199 kb on + strand, within Psyr_0824 at 933.200 kb on - strand, within Psyr_0824 at 933.200 kb on - strand, within Psyr_0824 at 933.200 kb on - strand, within Psyr_0824 at 933.200 kb on - strand, within Psyr_0824 at 933.585 kb on + strand at 933.611 kb on - strand at 933.611 kb on - strand at 933.611 kb on - strand at 933.745 kb on + strand at 933.963 kb on + strand, within Psyr_0825 at 933.963 kb on + strand, within Psyr_0825 at 933.963 kb on + strand, within Psyr_0825 at 933.964 kb on - strand, within Psyr_0825 at 934.038 kb on + strand, within Psyr_0825 at 934.038 kb on + strand, within Psyr_0825 at 934.041 kb on + strand, within Psyr_0825 at 934.041 kb on + strand, within Psyr_0825 at 934.041 kb on + strand, within Psyr_0825 at 934.042 kb on - strand, within Psyr_0825 at 934.080 kb on + strand, within Psyr_0825 at 934.118 kb on + strand, within Psyr_0825 at 934.119 kb on - strand, within Psyr_0825 at 934.158 kb on + strand, within Psyr_0825 at 934.241 kb on - strand, within Psyr_0825 at 934.248 kb on + strand, within Psyr_0825 at 934.248 kb on + strand, within Psyr_0825 at 934.248 kb on + strand, within Psyr_0825 at 934.249 kb on - strand, within Psyr_0825 at 934.568 kb on + strand at 934.568 kb on + strand at 934.569 kb on - strand at 934.569 kb on - strand at 934.632 kb on + strand at 934.649 kb on + strand at 934.649 kb on + strand at 934.649 kb on + strand at 934.649 kb on + strand at 934.650 kb on - strand at 934.650 kb on - strand at 934.662 kb on - strand at 934.662 kb on - strand at 934.662 kb on - strand at 934.688 kb on + strand at 934.688 kb on + strand at 934.688 kb on + strand at 934.688 kb on + strand at 934.688 kb on + strand at 934.689 kb on - strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.719 kb on - strand at 934.719 kb on - strand at 934.719 kb on - strand at 934.719 kb on - strand at 934.802 kb on + strand at 934.802 kb on + strand at 934.802 kb on + strand at 934.802 kb on + strand at 934.802 kb on + strand at 934.845 kb on + strand at 934.845 kb on + strand at 934.846 kb on - strand at 934.949 kb on + strand at 934.949 kb on + strand at 934.950 kb on - strand at 934.950 kb on - strand at 935.016 kb on - strand at 935.016 kb on - strand at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.414 kb on + strand, within Psyr_0826 at 935.414 kb on + strand, within Psyr_0826 at 935.415 kb on - strand, within Psyr_0826 at 935.481 kb on + strand, within Psyr_0826
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 932,783 + Psyr_0824 0.34 -0.1 933,064 + Psyr_0824 0.57 +0.4 933,065 - Psyr_0824 0.57 +0.7 933,065 - Psyr_0824 0.57 +3.9 933,103 + Psyr_0824 0.61 -0.7 933,103 + Psyr_0824 0.61 -1.1 933,103 + Psyr_0824 0.61 +1.7 933,104 - Psyr_0824 0.61 -1.8 933,199 + Psyr_0824 0.69 +1.8 933,199 + Psyr_0824 0.69 +1.9 933,199 + Psyr_0824 0.69 +0.4 933,199 + Psyr_0824 0.69 -3.3 933,200 - Psyr_0824 0.69 +0.7 933,200 - Psyr_0824 0.69 +0.5 933,200 - Psyr_0824 0.69 -2.7 933,200 - Psyr_0824 0.69 -2.8 933,585 + -2.2 933,611 - -2.8 933,611 - -2.5 933,611 - +2.0 933,745 + -4.1 933,963 + Psyr_0825 0.33 -0.7 933,963 + Psyr_0825 0.33 -1.7 933,963 + Psyr_0825 0.33 -0.4 933,964 - Psyr_0825 0.33 -0.3 934,038 + Psyr_0825 0.42 -3.7 934,038 + Psyr_0825 0.42 -3.0 934,041 + Psyr_0825 0.42 -1.0 934,041 + Psyr_0825 0.42 -0.1 934,041 + Psyr_0825 0.42 -1.1 934,042 - Psyr_0825 0.42 -1.5 934,080 + Psyr_0825 0.47 -3.6 934,118 + Psyr_0825 0.51 -0.2 934,119 - Psyr_0825 0.51 +0.6 934,158 + Psyr_0825 0.56 -0.8 934,241 - Psyr_0825 0.65 -1.8 934,248 + Psyr_0825 0.66 -3.0 934,248 + Psyr_0825 0.66 -1.4 934,248 + Psyr_0825 0.66 +2.3 934,249 - Psyr_0825 0.66 -2.2 934,568 + -2.8 934,568 + -1.8 934,569 - +1.4 934,569 - +0.9 934,632 + -1.5 934,649 + +0.3 934,649 + -0.2 934,649 + -3.1 934,649 + +0.2 934,650 - +1.4 934,650 - +1.3 934,662 - +3.5 934,662 - -1.6 934,662 - +1.3 934,688 + +0.8 934,688 + -3.8 934,688 + -1.8 934,688 + -3.1 934,688 + +2.1 934,689 - +3.3 934,701 + -2.8 934,701 + -2.3 934,701 + -2.6 934,701 + +1.1 934,701 + -0.2 934,701 + -2.3 934,701 + -2.8 934,701 + +1.4 934,701 + +1.2 934,701 + +0.9 934,702 - +1.2 934,702 - +1.7 934,702 - +2.4 934,702 - +1.1 934,702 - -0.4 934,702 - +0.3 934,702 - +1.7 934,702 - +0.9 934,702 - -0.8 934,718 + -3.4 934,718 + -3.4 934,718 + +0.9 934,718 + -1.6 934,718 + +0.0 934,718 + -0.4 934,718 + -0.3 934,718 + -1.4 934,718 + -0.7 934,718 + -1.6 934,718 + -0.8 934,718 + -1.7 934,718 + +0.5 934,718 + +0.4 934,719 - +0.7 934,719 - -2.0 934,719 - +1.6 934,719 - +0.7 934,802 + +0.5 934,802 + -0.0 934,802 + -2.6 934,802 + -1.0 934,802 + -0.3 934,845 + -0.4 934,845 + -0.4 934,846 - +0.6 934,949 + -2.1 934,949 + +0.5 934,950 - -1.5 934,950 - -1.8 935,016 - -1.5 935,016 - -1.4 935,111 + Psyr_0826 0.11 -2.1 935,111 + Psyr_0826 0.11 -1.0 935,111 + Psyr_0826 0.11 -1.3 935,111 + Psyr_0826 0.11 -2.8 935,111 + Psyr_0826 0.11 -2.1 935,111 + Psyr_0826 0.11 -0.0 935,111 + Psyr_0826 0.11 -1.4 935,111 + Psyr_0826 0.11 -1.7 935,111 + Psyr_0826 0.11 -1.0 935,111 + Psyr_0826 0.11 -1.6 935,111 + Psyr_0826 0.11 -1.5 935,111 + Psyr_0826 0.11 +0.8 935,111 + Psyr_0826 0.11 -1.2 935,111 + Psyr_0826 0.11 +0.1 935,112 - Psyr_0826 0.11 -1.8 935,112 - Psyr_0826 0.11 -1.3 935,112 - Psyr_0826 0.11 +1.8 935,112 - Psyr_0826 0.11 -1.7 935,112 - Psyr_0826 0.11 -2.8 935,112 - Psyr_0826 0.11 +3.1 935,112 - Psyr_0826 0.11 +0.5 935,112 - Psyr_0826 0.11 -1.0 935,414 + Psyr_0826 0.29 -1.5 935,414 + Psyr_0826 0.29 -1.8 935,415 - Psyr_0826 0.29 -2.9 935,481 + Psyr_0826 0.33 -2.9
Or see this region's nucleotide sequence