Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0792 and Psyr_0793 are separated by 90 nucleotides Psyr_0793 and Psyr_0794 are separated by 126 nucleotides Psyr_0794 and Psyr_0795 overlap by 4 nucleotides Psyr_0795 and Psyr_0796 overlap by 4 nucleotides
Psyr_0792: Psyr_0792 - conserved hypothetical protein, at 899,245 to 899,874
_0792
Psyr_0793: Psyr_0793 - conserved hypothetical protein, at 899,965 to 900,462
_0793
Psyr_0794: Psyr_0794 - Protein of unknown function DUF329, at 900,589 to 900,798
_0794
Psyr_0795: Psyr_0795 - dephospho-CoA kinase, at 900,795 to 901,418
_0795
Psyr_0796: Psyr_0796 - type 4 prepilin peptidase 1, Aspartic peptidase, MEROPS family A24A, at 901,415 to 902,287
_0796
Position (kb)
900
901 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 899.638 kb on - strand, within Psyr_0792 at 899.737 kb on - strand, within Psyr_0792 at 899.858 kb on + strand at 899.858 kb on + strand at 899.886 kb on + strand at 899.886 kb on + strand at 899.929 kb on + strand at 899.929 kb on + strand at 899.929 kb on + strand at 899.929 kb on + strand at 899.929 kb on + strand at 899.930 kb on - strand at 899.930 kb on - strand at 899.962 kb on + strand at 899.986 kb on - strand at 900.100 kb on + strand, within Psyr_0793 at 900.112 kb on + strand, within Psyr_0793 at 900.112 kb on + strand, within Psyr_0793 at 900.112 kb on + strand, within Psyr_0793 at 900.112 kb on + strand, within Psyr_0793 at 900.113 kb on - strand, within Psyr_0793 at 900.302 kb on - strand, within Psyr_0793 at 900.302 kb on - strand, within Psyr_0793 at 900.367 kb on - strand, within Psyr_0793 at 900.380 kb on + strand, within Psyr_0793 at 900.381 kb on - strand, within Psyr_0793 at 900.456 kb on + strand at 900.456 kb on + strand at 900.456 kb on + strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.457 kb on - strand at 900.469 kb on + strand at 900.469 kb on + strand at 900.470 kb on - strand at 900.470 kb on - strand at 900.470 kb on - strand at 900.470 kb on - strand at 900.470 kb on - strand at 900.550 kb on + strand at 900.550 kb on + strand at 900.550 kb on + strand at 900.550 kb on + strand at 900.551 kb on - strand at 900.551 kb on - strand at 900.551 kb on - strand at 900.558 kb on + strand at 900.558 kb on + strand at 900.558 kb on + strand at 900.558 kb on + strand at 900.558 kb on + strand at 900.558 kb on + strand at 900.558 kb on + strand at 900.558 kb on + strand at 900.559 kb on - strand at 900.559 kb on - strand at 900.559 kb on - strand at 900.559 kb on - strand at 900.559 kb on - strand at 900.559 kb on - strand at 900.559 kb on - strand at 900.561 kb on - strand at 900.561 kb on - strand at 900.561 kb on - strand at 900.588 kb on - strand at 900.588 kb on - strand at 900.818 kb on + strand at 900.818 kb on + strand at 900.818 kb on + strand at 900.819 kb on - strand at 901.441 kb on - strand at 901.441 kb on - strand at 901.441 kb on - strand at 901.441 kb on - strand at 901.441 kb on - strand at 901.441 kb on - strand at 901.441 kb on - strand at 901.441 kb on - strand at 901.441 kb on - strand at 901.452 kb on - strand at 901.452 kb on - strand at 901.497 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 899,638 - Psyr_0792 0.62 +2.2 899,737 - Psyr_0792 0.78 +0.0 899,858 + -0.4 899,858 + -3.5 899,886 + -2.5 899,886 + +0.8 899,929 + +0.5 899,929 + +0.2 899,929 + +1.4 899,929 + -0.4 899,929 + +1.9 899,930 - +1.7 899,930 - +0.8 899,962 + -0.4 899,986 - -0.9 900,100 + Psyr_0793 0.27 -2.2 900,112 + Psyr_0793 0.30 -0.5 900,112 + Psyr_0793 0.30 +1.9 900,112 + Psyr_0793 0.30 +1.4 900,112 + Psyr_0793 0.30 +0.0 900,113 - Psyr_0793 0.30 +0.5 900,302 - Psyr_0793 0.68 +0.1 900,302 - Psyr_0793 0.68 +1.5 900,367 - Psyr_0793 0.81 +2.0 900,380 + Psyr_0793 0.83 +0.3 900,381 - Psyr_0793 0.84 +3.0 900,456 + -1.1 900,456 + -3.7 900,456 + -1.2 900,457 - -0.7 900,457 - -0.4 900,457 - -1.7 900,457 - +3.6 900,457 - -3.3 900,457 - -0.8 900,457 - -1.4 900,457 - +0.9 900,457 - +3.1 900,457 - -1.7 900,469 + +1.0 900,469 + +1.8 900,470 - -2.3 900,470 - +1.7 900,470 - -1.5 900,470 - +4.6 900,470 - -2.6 900,550 + +1.4 900,550 + -1.5 900,550 + -1.0 900,550 + -1.8 900,551 - +1.1 900,551 - +0.3 900,551 - -0.3 900,558 + -3.4 900,558 + +0.7 900,558 + -2.3 900,558 + +1.2 900,558 + +2.3 900,558 + -2.2 900,558 + -2.9 900,558 + -0.9 900,559 - +0.5 900,559 - -2.6 900,559 - -2.6 900,559 - +0.6 900,559 - -0.0 900,559 - +1.2 900,559 - +0.9 900,561 - +0.1 900,561 - -1.7 900,561 - +2.1 900,588 - -4.3 900,588 - -1.7 900,818 + -3.7 900,818 + -1.1 900,818 + +2.1 900,819 - +2.2 901,441 - -1.6 901,441 - -1.2 901,441 - -0.1 901,441 - -2.0 901,441 - -0.9 901,441 - -1.5 901,441 - +1.0 901,441 - -1.7 901,441 - -1.1 901,452 - -0.4 901,452 - +0.3 901,497 + +2.4
Or see this region's nucleotide sequence