Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0589 and Psyr_0590 overlap by 1 nucleotides Psyr_0590 and Psyr_0591 are separated by 152 nucleotides Psyr_0591 and Psyr_0592 are separated by 77 nucleotides
Psyr_0589: Psyr_0589 - protein of unknown function DUF403:Protein of unknown function DUF404:Protein of unknown function DUF407, at 676,492 to 678,987
_0589
Psyr_0590: Psyr_0590 - Transglutaminase-like protein, at 678,987 to 679,880
_0590
Psyr_0591: Psyr_0591 - Blue (type 1) copper domain protein, at 680,033 to 680,479
_0591
Psyr_0592: Psyr_0592 - Integrase, catalytic region, at 680,557 to 681,393
_0592
Position (kb) 678
679
680 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 677.989 kb on + strand, within Psyr_0589 at 678.013 kb on + strand, within Psyr_0589 at 678.014 kb on - strand, within Psyr_0589 at 678.112 kb on + strand, within Psyr_0589 at 678.113 kb on - strand, within Psyr_0589 at 678.113 kb on - strand, within Psyr_0589 at 678.312 kb on - strand, within Psyr_0589 at 678.619 kb on + strand, within Psyr_0589 at 678.619 kb on + strand, within Psyr_0589 at 678.619 kb on + strand, within Psyr_0589 at 678.631 kb on + strand, within Psyr_0589 at 678.631 kb on + strand, within Psyr_0589 at 678.632 kb on - strand, within Psyr_0589 at 678.632 kb on - strand, within Psyr_0589 at 678.774 kb on + strand at 678.774 kb on + strand at 678.774 kb on + strand at 678.775 kb on - strand at 679.000 kb on - strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.024 kb on - strand at 679.029 kb on + strand at 679.029 kb on + strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.309 kb on - strand, within Psyr_0590 at 679.309 kb on - strand, within Psyr_0590 at 679.447 kb on + strand, within Psyr_0590 at 679.448 kb on - strand, within Psyr_0590 at 679.611 kb on + strand, within Psyr_0590 at 679.612 kb on - strand, within Psyr_0590 at 679.696 kb on + strand, within Psyr_0590 at 679.697 kb on - strand, within Psyr_0590 at 679.878 kb on + strand at 679.878 kb on + strand at 679.882 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.990 kb on + strand at 679.991 kb on - strand at 679.991 kb on - strand at 679.991 kb on - strand at 679.991 kb on - strand at 679.991 kb on - strand at 679.991 kb on - strand at 679.991 kb on - strand at 679.991 kb on - strand at 679.991 kb on - strand at 680.001 kb on + strand at 680.001 kb on + strand at 680.002 kb on - strand at 680.135 kb on + strand, within Psyr_0591 at 680.135 kb on + strand, within Psyr_0591 at 680.135 kb on + strand, within Psyr_0591 at 680.135 kb on + strand, within Psyr_0591 at 680.135 kb on + strand, within Psyr_0591 at 680.135 kb on + strand, within Psyr_0591 at 680.135 kb on + strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.136 kb on - strand, within Psyr_0591 at 680.230 kb on + strand, within Psyr_0591 at 680.231 kb on - strand, within Psyr_0591 at 680.306 kb on + strand, within Psyr_0591 at 680.306 kb on + strand, within Psyr_0591 at 680.306 kb on + strand, within Psyr_0591 at 680.306 kb on + strand, within Psyr_0591 at 680.307 kb on - strand, within Psyr_0591 at 680.307 kb on - strand, within Psyr_0591 at 680.414 kb on + strand, within Psyr_0591 at 680.415 kb on - strand, within Psyr_0591 at 680.477 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 677,989 + Psyr_0589 0.60 -0.1 678,013 + Psyr_0589 0.61 -0.9 678,014 - Psyr_0589 0.61 -2.9 678,112 + Psyr_0589 0.65 -1.3 678,113 - Psyr_0589 0.65 -1.6 678,113 - Psyr_0589 0.65 +0.9 678,312 - Psyr_0589 0.73 -0.9 678,619 + Psyr_0589 0.85 +0.9 678,619 + Psyr_0589 0.85 +0.1 678,619 + Psyr_0589 0.85 +1.0 678,631 + Psyr_0589 0.86 +2.2 678,631 + Psyr_0589 0.86 -1.4 678,632 - Psyr_0589 0.86 +0.1 678,632 - Psyr_0589 0.86 +1.1 678,774 + -2.1 678,774 + +1.1 678,774 + -0.2 678,775 - -0.0 679,000 - -3.4 679,023 + -2.7 679,023 + -2.2 679,023 + +0.6 679,023 + -2.4 679,023 + +0.2 679,023 + -1.9 679,023 + -0.9 679,024 - +0.5 679,029 + +1.3 679,029 + -4.2 679,030 - +0.7 679,030 - +1.4 679,030 - -0.5 679,030 - -1.2 679,030 - -1.9 679,030 - +1.5 679,308 + Psyr_0590 0.36 -2.4 679,308 + Psyr_0590 0.36 -2.0 679,308 + Psyr_0590 0.36 +0.5 679,308 + Psyr_0590 0.36 +1.1 679,308 + Psyr_0590 0.36 -1.1 679,308 + Psyr_0590 0.36 +7.4 679,309 - Psyr_0590 0.36 +0.7 679,309 - Psyr_0590 0.36 +1.6 679,447 + Psyr_0590 0.51 -2.2 679,448 - Psyr_0590 0.52 -2.0 679,611 + Psyr_0590 0.70 -2.6 679,612 - Psyr_0590 0.70 -3.0 679,696 + Psyr_0590 0.79 +3.0 679,697 - Psyr_0590 0.79 +0.1 679,878 + -2.4 679,878 + +1.7 679,882 + +0.7 679,990 + +1.5 679,990 + +1.4 679,990 + -0.7 679,990 + -1.1 679,990 + -1.9 679,990 + +0.1 679,990 + +0.4 679,990 + +0.6 679,990 + -0.0 679,990 + +0.2 679,990 + -0.3 679,991 - +1.8 679,991 - -1.4 679,991 - -3.3 679,991 - -1.6 679,991 - +0.4 679,991 - +0.8 679,991 - +0.8 679,991 - +0.0 679,991 - +3.1 680,001 + +1.2 680,001 + +0.8 680,002 - +1.1 680,135 + Psyr_0591 0.23 -4.0 680,135 + Psyr_0591 0.23 +0.4 680,135 + Psyr_0591 0.23 -1.2 680,135 + Psyr_0591 0.23 -1.1 680,135 + Psyr_0591 0.23 -1.1 680,135 + Psyr_0591 0.23 +1.1 680,135 + Psyr_0591 0.23 -2.1 680,136 - Psyr_0591 0.23 -3.2 680,136 - Psyr_0591 0.23 -0.4 680,136 - Psyr_0591 0.23 +1.0 680,136 - Psyr_0591 0.23 -1.8 680,136 - Psyr_0591 0.23 -0.1 680,136 - Psyr_0591 0.23 +0.6 680,136 - Psyr_0591 0.23 +2.7 680,136 - Psyr_0591 0.23 -0.9 680,136 - Psyr_0591 0.23 +0.1 680,230 + Psyr_0591 0.44 +1.6 680,231 - Psyr_0591 0.44 -2.0 680,306 + Psyr_0591 0.61 -3.8 680,306 + Psyr_0591 0.61 -0.5 680,306 + Psyr_0591 0.61 -1.2 680,306 + Psyr_0591 0.61 -2.5 680,307 - Psyr_0591 0.61 +1.5 680,307 - Psyr_0591 0.61 +0.8 680,414 + Psyr_0591 0.85 +2.9 680,415 - Psyr_0591 0.85 -1.6 680,477 + -3.0
Or see this region's nucleotide sequence