Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0580 and Psyr_0581 are separated by 257 nucleotides Psyr_0581 and Psyr_0582 are separated by 28 nucleotides Psyr_0582 and Psyr_0583 are separated by 39 nucleotides Psyr_0583 and Psyr_0584 are separated by 19 nucleotides Psyr_0584 and Psyr_0585 are separated by 123 nucleotides
Psyr_0580: Psyr_0580 - 23S rRNA Gm-2251 2'-O-methyltransferase, at 662,568 to 663,323
_0580
Psyr_0581: Psyr_0581 - SSU ribosomal protein S6P, at 663,581 to 664,006
_0581
Psyr_0582: Psyr_0582 - SSU ribosomal protein S18P, at 664,035 to 664,265
_0582
Psyr_0583: Psyr_0583 - membrane protein, putative, at 664,305 to 665,201
_0583
Psyr_0584: Psyr_0584 - LSU ribosomal protein L9P, at 665,221 to 665,667
_0584
Psyr_0585: Psyr_0585 - primary replicative DNA helicase, at 665,791 to 667,185
_0585
Position (kb) 664
665
666 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 663.321 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.376 kb on + strand at 663.376 kb on + strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.382 kb on + strand at 663.383 kb on - strand at 663.383 kb on - strand at 663.421 kb on + strand at 663.422 kb on - strand at 663.422 kb on - strand at 664.614 kb on + strand, within Psyr_0583 at 664.614 kb on + strand, within Psyr_0583 at 664.614 kb on + strand, within Psyr_0583 at 664.621 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.964 kb on + strand, within Psyr_0583 at 664.964 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.113 kb on + strand at 665.206 kb on + strand at 665.301 kb on + strand, within Psyr_0584 at 665.305 kb on + strand, within Psyr_0584 at 665.454 kb on + strand, within Psyr_0584 at 665.630 kb on + strand at 665.665 kb on + strand at 665.676 kb on + strand at 665.729 kb on + strand at 665.729 kb on + strand at 665.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 663,321 + -1.2 663,324 + -2.2 663,324 + +8.0 663,324 + +2.7 663,324 + +2.1 663,352 + +0.7 663,352 + -2.1 663,352 + +0.2 663,352 + -2.8 663,352 + -1.2 663,352 + -1.8 663,353 - -3.2 663,353 - -2.3 663,353 - +0.8 663,353 - +1.8 663,359 + +0.0 663,359 + -2.8 663,359 + +0.9 663,359 + -2.1 663,360 - +2.0 663,360 - +0.8 663,360 - -1.7 663,360 - -2.3 663,360 - -2.1 663,360 - -2.1 663,360 - -1.6 663,376 + +0.6 663,376 + +1.0 663,377 - +0.5 663,377 - -2.6 663,377 - -0.2 663,377 - +0.1 663,377 - +1.7 663,382 + +1.4 663,383 - +0.4 663,383 - -3.3 663,421 + +1.9 663,422 - +0.8 663,422 - +0.1 664,614 + Psyr_0583 0.34 +2.7 664,614 + Psyr_0583 0.34 -0.5 664,614 + Psyr_0583 0.34 +1.4 664,621 + Psyr_0583 0.35 -1.5 664,723 + Psyr_0583 0.47 -0.9 664,723 + Psyr_0583 0.47 -2.1 664,723 + Psyr_0583 0.47 -1.6 664,845 + Psyr_0583 0.60 -1.2 664,845 + Psyr_0583 0.60 -0.5 664,845 + Psyr_0583 0.60 +0.5 664,845 + Psyr_0583 0.60 +0.9 664,845 + Psyr_0583 0.60 -1.9 664,845 + Psyr_0583 0.60 -1.9 664,964 + Psyr_0583 0.73 +1.8 664,964 + Psyr_0583 0.73 +1.4 665,064 + Psyr_0583 0.85 +1.0 665,064 + Psyr_0583 0.85 +1.6 665,064 + Psyr_0583 0.85 +1.3 665,064 + Psyr_0583 0.85 -1.3 665,064 + Psyr_0583 0.85 +0.2 665,064 + Psyr_0583 0.85 -0.5 665,064 + Psyr_0583 0.85 +0.2 665,064 + Psyr_0583 0.85 +1.9 665,064 + Psyr_0583 0.85 -0.9 665,064 + Psyr_0583 0.85 -2.2 665,064 + Psyr_0583 0.85 +0.8 665,064 + Psyr_0583 0.85 -1.4 665,064 + Psyr_0583 0.85 -0.1 665,064 + Psyr_0583 0.85 +1.1 665,064 + Psyr_0583 0.85 +2.5 665,064 + Psyr_0583 0.85 +0.4 665,113 + +0.9 665,206 + +0.4 665,301 + Psyr_0584 0.18 -1.0 665,305 + Psyr_0584 0.19 -1.3 665,454 + Psyr_0584 0.52 -0.7 665,630 + -1.1 665,665 + +0.5 665,676 + -2.6 665,729 + +0.5 665,729 + +1.2 665,729 + +2.8
Or see this region's nucleotide sequence